DNASU Plasmid | DUSP24 (Homo sapiens) in pCR4zeroBLUNT-TOPO (TOPO cloning vector)

Explanation of Terms

Gene:

Gene Symbol: STYXL1

Gene Name: serine/threonine/tyrosine interacting-like 1

Original Clone ID:

8670a1ZBt31

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pCR4zeroBLUNT-TOPO

Format:

FUSION

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

"Invitrogen Zero Blunt, TOPO"

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00285713

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 936nts

Open reading frame: 12 to 947

Reference Sequence Alignment

GAATCGCCCTTCCTGGTTTGCTTTTATGTGAACCAACAGAGCTTTACAACATCCTGAATC
AGGCCACAAAACTCTCCAGATTAACAGACCCCAACTATCTCTGTTTATTGGATGTCCGTT
CCAAATGGGAGTATGACGAAAGCCATGTGATCACTGCCCTTCGAGTGAAGAAGAAAAATA
ATGAATATCTTCTCCCGGAGTCTGTGGACCTGGAGTGTGTGAAGTACTGCGTGGTGTATG
ATAACAACAGCAGCACCCTGGAGATACTCTTAAAAGATGATGATGATGATTCAGACTCTG
ATGGTGATGGCAAAGATCTTGTGCCTCAAGCAGCCATTGAGTATGGCAGGATCCTGACCC
GCCTCACCCACCACCCCGTCTACATCCTGAAAGGGGGCTATGAGCGCTTCTCAGGCACGT
ACCACTTTCTCCGGACCCAGAAGATCATCTGGATGCCTCAGGAACTGGATGCATTTCAGC
CATACCCCATTGAAATCGTGCCAGGGAAGGTCTTCGTTGGCAATTTCAGTCAAGCCTGTG
ACCCCAAGATTCAGAAGGACTTGAAAATCAAAGCCCATGTCAATGTCTCCATGGATACAG
GGCCCTTTTTTGCAGGCGATGCTGACAAGCTTCTGCACATCCGGATAGAAGATTCCCCGG
AAGCCCAGATTCTTCCCTTCTTACGCCACATGTGTCACTTCATTGAAATTCACCATCACC
TTGGCTCTGTCATTCTGATCTTTTCCACCCAAGGTATCAGCCGCAGTTGTGCCGCCATCA
TAGCCTACCTCATGCATAGTAACGAGCAGACCTTGCAGAGGTCCTGGGCCTATGTCAAGA
AGTGCAAAAACAACATGTGTCCAAATCGGGGATTGGTGAGCCAGCTGCTGGAATGGGAGA
AGACTATCCTTGGAGATTCCATCACAAACATCATGGATCCGCTCTACAAGGGGCGAATT

Protein sequence predicted based on nucleotide sequence

PGLLLCEPTELYNILNQATKLSRLTDPNYLCLLDVRSKWEYDESHVITALRVKKKNNEYL
LPESVDLECVKYCVVYDNNSSTLEILLKDDDDDSDSDGDGKDLVPQAAIEYGRILTRLTH
HPVYILKGGYERFSGTYHFLRTQKIIWMPQELDAFQPYPIEIVPGKVFVGNFSQACDPKI
QKDLKIKAHVNVSMDTGPFFAGDADKLLHIRIEDSPEAQILPFLRHMCHFIEIHHHLGSV
ILIFSTQGISRSCAAIIAYLMHSNEQTLQRSWAYVKKCKNNMCPNRGLVSQLLEWEKTIL
GDSITNIMDPLY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 1

End on reference sequence 936

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50 ug/mL ampicillin

Growth Condition: Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL)

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in NYSGXRC-8670a

Recommended expression in:

Not Applicable

Vector Name:

pCR4zeroBLUNT-TOPO

Synonyms:

pCR-4, pCR-IV, pCR4, pCR4-TOPO

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

TOPO cloning vector with M13 forward/reverse, T3, and T7 primers. Ampicillin and kanamycin resistance bacteria.

Comments:

Empty vector is a product available from Invitrogen.

Size (bp):

3956

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTAAGGGCGAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAACGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC bacterial origin of replication

3161

3834

primer site

M13 reverse

M13 reverse priming site

205

221

primer site

T3 priming site

243

262

primer site

M13 forward

M13 forward priming site

355

370

primer site

T7 priming site

328

347

promoter

lac

lac promoter region

216

selectable marker

AmpR

Ampicillin resistance gene

2203

3063

selectable marker

KanR

Kanamycin resistance gene

1159

1953

Gene

Gene Symbol :

STYXL1

Symbol Nomenclature :

STYXL1

SYNONYM :

DUSP24; MK-STYX

Designation :

dual specificity phosphatase 24 (putative)|dual specificity phosphatase inhibitor MK-STYX|dual specificity protein phosphatase 24|map kinase phosphatase-like protein MK-STYX|serine/threonine/tyrosine-interacting-like protein 1

Full Nomenclature :

serine/threonine/tyrosine interacting-like 1

GENEID :

51657

HGNC :

18165

Vega :

OTTHUMG00000130459

Target GenBank:

NP_057170

Reference Sequence Alignment

  

HsCD00285713      GAATCGCCCTTCCTGGTTTGCTTTTATGTGAACCAACAGAGCTTTACAACATCCTGAATC
NM_016086.2       --------ATGCCTGGTTTGCTTTTATGTGAACCAACAGAGCTTTACAACATCCTGAATC
                          .* *************************************************

HsCD00285713      AGGCCACAAAACTCTCCAGATTAACAGACCCCAACTATCTCTGTTTATTGGATGTCCGTT
NM_016086.2       AGGCCACAAAACTCTCCAGATTAACAGACCCCAACTATCTCTGTTTATTGGATGTCCGTT
                  ************************************************************

HsCD00285713      CCAAATGGGAGTATGACGAAAGCCATGTGATCACTGCCCTTCGAGTGAAGAAGAAAAATA
NM_016086.2       CCAAATGGGAGTATGACGAAAGCCATGTGATCACTGCCCTTCGAGTGAAGAAGAAAAATA
                  ************************************************************

HsCD00285713      ATGAATATCTTCTCCCGGAGTCTGTGGACCTGGAGTGTGTGAAGTACTGCGTGGTGTATG
NM_016086.2       ATGAATATCTTCTCCCGGAGTCTGTGGACCTGGAGTGTGTGAAGTACTGCGTGGTGTATG
                  ************************************************************

HsCD00285713      ATAACAACAGCAGCACCCTGGAGATACTCTTAAAAGATGATGATGATGATTCAGACTCTG
NM_016086.2       ATAACAACAGCAGCACCCTGGAGATACTCTTAAAAGATGATGATGATGATTCAGACTCTG
                  ************************************************************

HsCD00285713      ATGGTGATGGCAAAGATCTTGTGCCTCAAGCAGCCATTGAGTATGGCAGGATCCTGACCC
NM_016086.2       ATGGTGATGGCAAAGATCTTGTGCCTCAAGCAGCCATTGAGTATGGCAGGATCCTGACCC
                  ************************************************************

HsCD00285713      GCCTCACCCACCACCCCGTCTACATCCTGAAAGGGGGCTATGAGCGCTTCTCAGGCACGT
NM_016086.2       GCCTCACCCACCACCCCGTCTACATCCTGAAAGGGGGCTATGAGCGCTTCTCAGGCACGT
                  ************************************************************

HsCD00285713      ACCACTTTCTCCGGACCCAGAAGATCATCTGGATGCCTCAGGAACTGGATGCATTTCAGC
NM_016086.2       ACCACTTTCTCCGGACCCAGAAGATCATCTGGATGCCTCAGGAACTGGATGCATTTCAGC
                  ************************************************************

HsCD00285713      CATACCCCATTGAAATCGTGCCAGGGAAGGTCTTCGTTGGCAATTTCAGTCAAGCCTGTG
NM_016086.2       CATACCCCATTGAAATCGTGCCAGGGAAGGTCTTCGTTGGCAATTTCAGTCAAGCCTGTG
                  ************************************************************

HsCD00285713      ACCCCAAGATTCAGAAGGACTTGAAAATCAAAGCCCATGTCAATGTCTCCATGGATACAG
NM_016086.2       ACCCCAAGATTCAGAAGGACTTGAAAATCAAAGCCCATGTCAATGTCTCCATGGATACAG
                  ************************************************************

HsCD00285713      GGCCCTTTTTTGCAGGCGATGCTGACAAGCTTCTGCACATCCGGATAGAAGATTCCCCGG
NM_016086.2       GGCCCTTTTTTGCAGGCGATGCTGACAAGCTTCTGCACATCCGGATAGAAGATTCCCCGG
                  ************************************************************

HsCD00285713      AAGCCCAGATTCTTCCCTTCTTACGCCACATGTGTCACTTCATTGAAATTCACCATCACC
NM_016086.2       AAGCCCAGATTCTTCCCTTCTTACGCCACATGTGTCACTTCATTGAAATTCACCATCACC
                  ************************************************************

HsCD00285713      TTGGCTCTGTCATTCTGATCTTTTCCACCCAAGGTATCAGCCGCAGTTGTGCCGCCATCA
NM_016086.2       TTGGCTCTGTCATTCTGATCTTTTCCACCCAAGGTATCAGCCGCAGTTGTGCCGCCATCA
                  ************************************************************

HsCD00285713      TAGCCTACCTCATGCATAGTAACGAGCAGACCTTGCAGAGGTCCTGGGCCTATGTCAAGA
NM_016086.2       TAGCCTACCTCATGCATAGTAACGAGCAGACCTTGCAGAGGTCCTGGGCCTATGTCAAGA
                  ************************************************************

HsCD00285713      AGTGCAAAAACAACATGTGTCCAAATCGGGGATTGGTGAGCCAGCTGCTGGAATGGGAGA
NM_016086.2       AGTGCAAAAACAACATGTGTCCAAATCGGGGATTGGTGAGCCAGCTGCTGGAATGGGAGA
                  ************************************************************

HsCD00285713      AGACTATCCTTGGAGATTCCATCACAAACATCATGGATCCGCTCTACAAGGGGCGAATT
NM_016086.2       AGACTATCCTTGGAGATTCCATCACAAACATCATGGATCCGCTCTACTGA---------
                  ***********************************************:..