Detailed Vector Information: pSpeedET

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pSpeedET
Source:
Joint Center for Structural Genomics
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments:
Empty vector contains ccdB death cassette.
Publications:
PMID: 26626129
Title: Molecular insights into the enzymatic diversity of flavin-trafficking protein (Ftp; formerly ApbE) in flavoprotein biogenesis in the bacterial periplasm
Authors:
PSI
Joint Center for Structural Genomics
Genomics Institute of the Novartis Research Foundation
EvNO00285791
Price: 
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No restriction
Special MTA: PSI

Recommended Growth Condition:

Distributed in bacterial strain:
DB3.1 T1 phage resistant cells
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.