smC (B. anthracis str. Ames )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
FLH263088.01L
Keyword:
None
Species:
Bacillus anthracis str. Ames
Type:
cDNA
Vector Name:
pDONR221
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HIP
BaCD00286748
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3570nts
Open reading frame: 1 to 3570

ATGTTTTTAAAAAGATTAGAAATAGCAGTATTTAAGTCTTTTGCTGAACGTGTATCTGTC
GATTTCGTCCCAGGTGTAACGTCTGTAGTAGGACCTAATGGAAGCGGGAAAAGTAATATT
ACGGATGCCATTCGCTGGGTACTTGGTGAACAATCGGCAAAATCATTACGTGGTGCAAAG
ATGGAAGATATTATTTTTGCAGGTAGTGATACGAGAAGAGCGGTTAATGTTGCTGAAGTA
ACAATAACTTTAAATAATGAAGATCAACGTTTACCAATTGAATATAATGAGGTGTGTGTA
ACGCGTCGTGTATCTCGTTCTGGTGATAGTGATTTTTATATTAATAAACAATCATGCAGG
TTGAAAGATATTATCGATTTATTTATGGACTCTGGTATGGGAAGAGAAGCTTTTTCGATT
ATTAGCCAGGGGAAAGTTGAAGAGATTTTAAGTAGTAAATCAGAAGAACGTCGTGGTGTA
TTTGAAGAAGCGGCGGGAGTGCTGAAATACAAACTTCGTAAAAAGAAAGCTGAAAGTAAA
TTAGCAGACACACAAGAAAACTTAAATCGTGTACAAGATATAATTCACGAATTAAGTAGT
CAAGTAGAACCTTTAGAAAGACAAGCTTCTATTGCAAAGGATTATCTTGAGAAAAAAGAA
GAATTAGAGAAAGTAGAAGCAGCGCTTATTGTACATGAAATTGAAGAATTGCATGAGAAA
TGGGAAGCGCTTCGAAATCAGTTTGGGCATAATAAAAACGAAGAAGCTAAAATGTCAACG
CATTTACAAAAAGGTGAAGAAGAGTTAGAGGAATTACGAGGCCAATTACAAGCGGTAGAT
GAGTCGGTAGATTCCCTGCAAGAAGTACTTTTACTTTCTAGTAAAGAGCTAGAAAAGTTA
GAAGGGCAGCGTGAGCTATTAAAAGAGAGAAAGCAAAATGCAACGACGCATTGTGCGCAA
CTTGAACAGTTAATTGTTGAATTAACAGAGAAAGCTACAAGTTATGATGGTGAAATCGAA
TCGAGTACAGAAGTGTTGATGCAATTTGTAAATCATGTAAAAGAGTTGGAGATGAAATTG
CATGATAATGAGCAATTGCTTGCAACGTTTGCTGATAATTTAGAGGAACAAATTGAGAAT
TTAAAAGGTGATTATATTGAACTTTTAAATCAACAAGCTAGTCATCGTAATGAGTTATCT
ATGATTGAAGAACAATCTAAACAACAAAATTCAAAAAATGAACGCCTTGATGAAGAAAAT
GCGAAATATGTAGAAATGCGTATGGAAATTACAGCGAAAAAGACAAAACTTGTGGAAAGT
TATGAACAAGTAAAAGAAAAAGTAGCTGGAATCCTTTCGAATATACAAAAGACAGAAGCG
GCACTTGGGAAATGCAAGGCGCAGTATAGTGAAAATGAAACAAAACTATACCAAGCGTAT
CAATTTGTACAACAGGCACGTTCTCGAAAAGAAATGCTAGAAGAGATGCAAGAAGATTAC
TCTGGTTTCTATCAAGGGGTACGTGAAGTATTAAAAGCTAGAGAAAATAGGTTGCAAGGT
ATCGAGGGGGCTGTTGCAGAATTGCTAACGGTACCGAAAGAATATGAAATTGCAATGGAA
ATTGCTCTAGGTGCAGCGATGCAGCATATCGTTGTACAAAAAGAAGAACATGCTCGTAAT
GCAATTGCGTTTTTAAAACAAAATAAACATGGACGAGCAACGTTTTTACCGCAGGCTGTT
ATGAAAGGTAGATCGTTATCATTTGAGCAATTACGTATTGTAAATCAACATCCATCGTTT
GTAGGTGTGGCAGCAGAACTTGTGCAATATAACAATAAATATGAAAATGTAGTTTCCAAT
TTATTAGGTACTGTTATTGTTGCAAAAGATTTACGCGGGGCGAATGAGTTGGCGAAACAA
TTGCAATACCGCTATCGCATTGTAACAATCGAAGGTGATGTAGTGAACCCTGGCGGTTCT
ATGACAGGTGGCGCAGTAAAACAGGCGAAATCTTCTTTATTAGGACGTCAACGTGAGCTA
GAAGAGTGGACGAACAAGTTAACTGATATGGAAGAAAAAACGACGAAGTTAGAAAACTTT
GTTAAAGCAGTAAAACAAGAGATTCAAGAAAAAGAAGTGCAAATACGCGAACTAAGGAAA
AGTGTAGAAGCAGAGCGTGTAGATGAACAGAAATTAAGAGAAGAAATTAATCGTTTAGAA
TTAGAAGAACATCGTATCAATGATCGTTTATCTATTTACGATTTAGAGATTGAAGGGTTC
TTACAAGATCAAGTGAAGATACAAGGGCGTAAAGAAGAGCTAGAAAAGATTTTAGCGACC
CTTCAAGCGGAGATTACGGGATTAGATAGCAAAATTGTCGCTTTAACACAACAAAAAAGT
GAGCAACATACGTCGAAAGAAAAAGTTCAAAAAGAAATGACTGAGTTAAAAGTGTTGGCG
GCTGAAAAGCAACAACGCTTATCTAATCAAAAAGAAAAAGTTGAACGATTGACGAAGGAA
AAAGAAGAAACGGATGCAACGCTTGTCAAAACAAAAGAGGATTTAGCATTCTTAAAACAA
GAAATGACATCGAATTCAAGTGGAGAAGAGCAAATTACGAATATGATTGAGAAGAAAGCG
TATGATCGTAATCAAACTTCGGAGTTAATTCGCTCTCGTCGAGAACAACGTGTATCGTTA
CAAGAGAGAGTAGAACAGTTAGAGCGTAATCTGAAAGAAACAACGGGTAAACATAAATAC
ATTCTTGAGATGTTGAAAGATCAAGAAGTAAAAATAAACCGACTTGATGTAGAGTTAGAA
AATAGATTACAACATTTACGTGAAACATATACGATTTCATTTGAAGCGGCAAAACTGAAG
TATACAATGATGATGCCTGCTGAGGATGCACGTAAAAAAGTGAAACTAATTAAACTATCC
ATCGAAGAGCTAGGCACAGTAAACTTAGGGGCAATTGATGAGTATGAGCGTGTAGCGGAG
CGTCATACATTCTTACTTGAGCAAAAAGATGATCTAGAAGAAGCAAAAACGACATTGCAC
CAACTTATTACTGAAATGGATGAAGAAATGAAAAAACGCTTTTCTACTACGTTTGAAGGG
ATTCGAATGGAGTTTCAATCGGTGTTCTCTGAATTGTTTGGAGGCGGTAGAGCGGATTTA
GTCATGACGAATCCAGAGGATTTATTAAATACGGGTATTGATATTGTAGCGCAACCGCCA
GGGAAGAAACTGCAAAACTTAGGTTTACTTTCAGGTGGAGAGCGTGCTTTAACGGCAATT
GCGCTATTATTTGGTATTTTAAAAGTGTGCCCAGTCCCATTCTGTGTATTAGATGAGGTC
GAGGCCGCTCTTGATGAGGCAAACGTTGCCCGTTTTGCCCAGTATTTAAAGAAATTTAGT
GATGAGACACAGTTTATTGTAATTACACATAGAAAAGGTACAATGGAAGAGTCTGACGTA
TTGTACGGTGTAACAATGCAAGAGTCAGGGGTATCTAAACTTGTCTCGGTTCGTTTAGAT
GATGGAGAAGAACTAGTCGCAAGCAAATTG

Protein sequence predicted based on nucleotide sequence

MFLKRLEIAVFKSFAERVSVDFVPGVTSVVGPNGSGKSNITDAIRWVLGEQSAKSLRGAK
MEDIIFAGSDTRRAVNVAEVTITLNNEDQRLPIEYNEVCVTRRVSRSGDSDFYINKQSCR
LKDIIDLFMDSGMGREAFSIISQGKVEEILSSKSEERRGVFEEAAGVLKYKLRKKKAESK
LADTQENLNRVQDIIHELSSQVEPLERQASIAKDYLEKKEELEKVEAALIVHEIEELHEK
WEALRNQFGHNKNEEAKMSTHLQKGEEELEELRGQLQAVDESVDSLQEVLLLSSKELEKL
EGQRELLKERKQNATTHCAQLEQLIVELTEKATSYDGEIESSTEVLMQFVNHVKELEMKL
HDNEQLLATFADNLEEQIENLKGDYIELLNQQASHRNELSMIEEQSKQQNSKNERLDEEN
AKYVEMRMEITAKKTKLVESYEQVKEKVAGILSNIQKTEAALGKCKAQYSENETKLYQAY
QFVQQARSRKEMLEEMQEDYSGFYQGVREVLKARENRLQGIEGAVAELLTVPKEYEIAME
IALGAAMQHIVVQKEEHARNAIAFLKQNKHGRATFLPQAVMKGRSLSFEQLRIVNQHPSF
VGVAAELVQYNNKYENVVSNLLGTVIVAKDLRGANELAKQLQYRYRIVTIEGDVVNPGGS
MTGGAVKQAKSSLLGRQRELEEWTNKLTDMEEKTTKLENFVKAVKQEIQEKEVQIRELRK
SVEAERVDEQKLREEINRLELEEHRINDRLSIYDLEIEGFLQDQVKIQGRKEELEKILAT
LQAEITGLDSKIVALTQQKSEQHTSKEKVQKEMTELKVLAAEKQQRLSNQKEKVERLTKE
KEETDATLVKTKEDLAFLKQEMTSNSSGEEQITNMIEKKAYDRNQTSELIRSRREQRVSL
QERVEQLERNLKETTGKHKYILEMLKDQEVKINRLDVELENRLQHLRETYTISFEAAKLK
YTMMMPAEDARKKVKLIKLSIEELGTVNLGAIDEYERVAERHTFLLEQKDDLEEAKTTLH
QLITEMDEEMKKRFSTTFEGIRMEFQSVFSELFGGGRADLVMTNPEDLLNTGIDIVAQPP
GKKLQNLGLLSGGERALTAIALLFGILKVCPVPFCVLDEVEAALDEANVARFAQYLKKFS
DETQFIVITHRKGTMEESDVLYGVTMQESGVSKLVSVRLDDGEELVASKL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3570
Mutations:
None
Discrepancy:
g29t,G10V; a2277g;
 c3328t,R1110C
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable