CFTR (H. sapiens )
In pFASTBAC1-C-His-TOPO (His-tagged baculovirus expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CFTR
Gene Name:  cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Original Clone ID:
2935c552KWg2h2
Keyword:
Partial Protein, NBD1 domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pFASTBAC1-C-His-TOPO
Format: 
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD1 domain with other intended mutations and corresponds to region 388-646 on full length protein NP_000483.3.
Comments:
C-terminal 6-His, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
Yes / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
HsCD00287209
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 816nts
Open reading frame: 1 to 816

ATGGCCCTTACAAGCTTAAAAGGACTATGGACACTTCGTGCCTTCGGACGGCAGCCTTCC
GGTGGTGGTAGCGGAGGAGGATCTGGCGGCGGCTCAGGGGGCGGGACGACTACAGAAGTA
GTGATGGAGAATGTAACAGCCTTCTGGGAGGAGGGAGGTACTCCTGTCCTGAAAGATATT
AATTTCAAGATAGAAAGAGGACAGTTGTTGGCGGTTGCTGGATCCACTGGAGCAGGCAAG
ACTTCACTTCTAATGATGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAAATTAAGCAC
AGTGGAAGAATTTCATTCTGTTCTCAGTTTTCCTGGATTATGCCTGGCACCATTAAAGAA
AATATCATCTTTGGTGTTTCCTATGATGAATATAGATACAGAAGCGTCATCAAAGCATGC
CAACTAGAAGAAGACATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGT
GGAATCACACTGAGTGGAGGTCAACGAGCAAGAATTTCTTTAGCAAGAGCAGTATACAAA
GATGCTGATTTGTATTTATTAGACTCTCCTTTTGGATACCTAGATGTTTTAACAGAAAAA
GAAATATTTGAAAGCTGTGTCTGTAAACTGATGGCTAACAAAACTAGGATTTTGGTCACT
TCTAAAATGGAACATTTAAAGAAAGCTGACAAAATATTAATTTTGCATGAAGGTAGCAGC
TATTTTTATGGGACATTTTCAGAACTCCAAAATCTACAGCCAGACTTTAGCTCAAAACTC
ATGGGAGAAGGGCATCATCACCATCACCACTGATAA

Protein sequence predicted based on nucleotide sequence

MALTSLKGLWTLRAFGRQPSGGGSGGGSGGGSGGGTTTEVVMENVTAFWEEGGTPVLKDI
NFKIERGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQFSWIMPGTIKE
NIIFGVSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYK
DADLYLLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSS
YFYGTFSELQNLQPDFSSKLMGEGHHHHHH**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 388
End on protein reference sequence 646
Mutations:
387ins31, del405-436, N620H*

ins@388,31

Discrepancy:
None
Others:
Termini 388-646
Domain NBD1
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Host Type:  insect cells    Marker:  gentamicin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 2935c
Recommended expression in:
Not Applicable