Gene:
Gene Symbol:
CFTR
Gene Name:
cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Original Clone ID:
5762c41KWg2h1
Keyword:
Partial Protein, NBD1 linked to NBD2 domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pFASTBAC1-C-His-TOPO
Format:
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD1 linked to NBD2 domain with other intended mutations and corresponds to region 388-1445 on full length protein NP_000483.3.
Comments:
C-terminal 6-His, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
Yes / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
HsCD00287211
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
ATGGCCCTTACGACTACAGAAGTAGTGATGGAGAATGTAACAGCCTTCTGGGAGGAGGGA
GGTACTCCTGTCCTGAAAGATATTAATTTCAAGATAGAAAGAGGACAGTTGTTGGCGGTT
GCTGGATCCACTGGAGCAGGCAAGACTTCACTTCTAATGATGATTATGGGAGAACTGGAG
CCTTCAGAGGGTAAAATTAAGCACAGTGGAAGAATTTCATTCTGTTCTCAGTTTTCCTGG
ATTATGCCTGGCACCATTAAAGAAAATATCATCTTTGGTGTTTCCTATGATGAATATAGA
TACAGAAGCGTCATCAAAGCATGCCAACTAGAAGAAGACATCTCCAAGTTTGCAGAGAAA
GACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGTGGAGGTCAACGAGCAAGAATT
TCTTTAGCAAGAGCAGTATACAAAGATGCTGATTTGTATTTATTAGACTCTCCTTTTGGA
TACCTAGATGTTTTAACAGAAAAAGAAATATTTGAAAGCTGTGTCTGTAAACTGATGGCT
AACAAAACTAGGATTTTGGTCACTTCTAAAATGGAACATTTAAAGAAAGCTGACAAAATA
TTAATTTTGCATGAAGGTAGCAGCTATTTTTATGGGACATTTTCAGAACTCCAAAATCTA
CAGCCAGACTTTAGCTCAAAACTCATGGGAGGTGGTGGTGGTAGCGGCGGCGGCGGCTCT
ATTGAGAATTCACACGTGAAGAAAGATGACATCTGGCCCTCAGGGGGCCAAATGACTGTC
AAAGATCTCACAGCAAAATACACAGAAGGTGGAAATGCCATATTAGAGAACATTTCCTTC
TCAATAAGTCCTGGCCAGAGGGTGGGCCTCTTGGGAAGAACTGGATCAGGGAAGAGTACT
TTGTTATCAGCTTTTTTGAGACTACTGAACACTGAAGGAGAAATCCAGATCGATGGTGTG
TCTTGGGATTCAATAACTTTGGAACAGTGGAGGAAAGCCTTTGGAGTGATACCACAGAAA
GTATTTATTTTTTCTGGAACATTTAGAAAAAACTTGGATCCCTATGAACAGTGGAGTGAC
CAAGAAATATGGAAAGTTGCAGATGAGGTTGGGCTCAGATCTGTGATAGAACAGTTTCCT
GGGAAGCTTGACTTTGTCCTTGTGGATGGGGGCTGTGTCCTAAGCCATGGCCACAAGCAG
TTGATGTGCTTGGCTAGATCTGTTCTCAGTAAGGCGAAGATCTTGCTGCTTGATGAACCC
AGTGCTCATTTGGATCCAGTAACATACCAAATAATTAGAAGAACTCTAAAACAAGCATTT
GCTGATTGCACAGTAATTCTCTGTGAAGCCAGGATAGAAGCAATGCTGGAATGCGATCAA
TTTTTGGTCATAGAAGAGAACAAAGTGCGGCAGTACGATTCCATCCAGAAACTGCTGAAC
GAGAGGAGCGACTTCCGGCAAGCCATCAGCCCCTCCGACGAAGGGCATCATCACCATCAC
CACTGATAGTAA
Protein sequence predicted based on nucleotide sequence
MALTTTEVVMENVTAFWEEGGTPVLKDINFKIERGQLLAVAGSTGAGKTSLLMMIMGELE PSEGKIKHSGRISFCSQFSWIMPGTIKENIIFGVSYDEYRYRSVIKACQLEEDISKFAEK DNIVLGEGGITLSGGQRARISLARAVYKDADLYLLDSPFGYLDVLTEKEIFESCVCKLMA NKTRILVTSKMEHLKKADKILILHEGSSYFYGTFSELQNLQPDFSSKLMGGGGGSGGGGS IENSHVKKDDIWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSGKST LLSAFLRLLNTEGEIQIDGVSWDSITLEQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSD QEIWKVADEVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEP SAHLDPVTYQIIRRTLKQAFADCTVILCEARIEAMLECDQFLVIEENKVRQYDSIQKLLN ERSDFRQAISPSDEGHHHHHH***
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
End on protein reference sequence
1445
Start on protein reference sequence
388
Mutations:
del@647,545
Discrepancy:
None
Others:
Termini
388-1445
Domain NBD1 linked to NBD2
Domain NBD1 linked to NBD2
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Host Type: insect cells Marker: gentamicin
Host Type: insect cells Marker: gentamicin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 5762c
Recommended expression in:
Not Applicable
Vector Name:
pFASTBAC1-C-His-TOPO
Synonyms:
pFB1-C-His-TOPO, pFB1-C-His-TOPO_KW
Sequencing Primer:
Forward:
pFB forward
Reverse:
pFB reverse
Description:
Baculovirus vector, adds C-terminal 6xHis tag and TEV protease site; amp resistance; TOPO cloning.
Comments:
None
Size (bp):
4763
Parent Vector:
None
Properties:
TOPO Cloning, baculovirus/insect cell expression, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation ATG (Met, Ala, Leu)
4065
4078
TOPO recognition site
TOPO
TOPO cloning site
4069
4078
bacterial origin
ori
pUC-type bacterial origin of replication
1544
2226
poly-A signal
polyA
SV40 polyA signal sequence
4189
4380
primer
pFB foward
forward sequencing primer: TATTCCGGATTATTCATACC
3995
4014
primer
pFB reverse
reverse sequencing primer: ACAAATGTGGTATGGCTGATT
4150
4170
promoter
PH
polyhedrin promoter for insect cell expression
3904
4032
selectable marker
GenR
gentamycin resistance gene
3000
3533
selectable marker
AmpR
ampicillin resistance gene
787
1446
ssDNA origin
f1 ori
f1 origin
19
325
tag
His
C-terminal 6xHis and stop
4079
4096
transposon fragment
Tn7L
Tn7L transposon site
1466
12
transposon fragment
Tn7R
Tn7R transposon site
2709
2933
