CFTR (Homo sapiens) in pSGX4

Explanation of Terms

Gene:
Gene Symbol:  CFTR
Gene Name:  cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Original Clone ID:
5762c54BSt1pi1
Keyword:
Partial Protein, NBD2 linked to NBD1 domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSGX4
Format: 
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD2 linked to NBD1 domain with other intended mutations and corresponds to region 1193-646 on full length protein NP_000483.3.
Comments:
N-terminal 6-His and Smt3, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
Yes / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
HsCD00287232
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Insert sequence: 1497nts
Open reading frame: 1 to 1497

ATTGAGAATTCACACGTGAAGAAAGATGACATCTGGCCCTCAGGGGGCCAAATGACTGTC
AAAGATCTCACAGCAAAATACACAGAAGGTGGAAATGCCATATTAGAGAACATTTCCTTC
TCAATAAGTCCTGGCCAGAGGGTGGGCCTCTTGGGAAGAACTGGATCAGGGAAGAGTACT
TTGTTATCAGCTTTTTTGAGACTACTGAATACTGAAGGAGAAATCCAGATCGATGGTGTG
TCTTGGGATTCAATAACTTTGGAACAGTGGAGGAAAGCCTTTGGAGTGATACCACAGAAA
GTATTTATTTTTTCTGGAACATTTAGAAAAAACTTGGATCCCAATGCAGCACATAGTGAC
CAAGAAATATGGAAAGTTGCAGATGAGGTTGGGCTCAGATCTGTGATAGAACAGTTTCCT
GGGAAGCTTGACTTTGTCCTTGTGGATGGGGGCTGTGTCCTAAGCCATGGCCACAAGCAG
TTGATGTGCTTGGCTAGATCTGTTCTCAGTAAGGCGAAGATCTTGCTGCTTGATGAACCC
AGTGCTCATTTGGATCCAGTAACATACCAAATAATTAGAAGAACTCTAAAACAAGCATTT
GCTGATTGCACAGTAATTCTCTGTGAAGCCAGGATAGAAGCAATGCTGGAATGCGATCAA
TTTTTGGTCATAGAAGAGAACAAAGTGCGGCAGTACGATTCCATCCAGAAACTGCTGAAC
GAGAGGAGCCTCTTCCGGCAAGCCATCAGCCCCTCCGACAGGCCGTATGAAATTAAATTT
ACCAAAACGGGCGAACGCGTGGAAATTGAACGCGAAACCCTGGTGACCATGGAGAATGTA
ACAGCCTTCTGGGAGGAGGGAGGTACTCCTGTCCTGAAAGATATTAATTTCAAGATAGAA
AGAGGACAGTTGTTGGCGGTTGCTGGATCCACTGGAGCAGGCAAGACTTCACTTCTAATG
ATGATTATGGGAGAACTGGAGCCTTCAGAGGGTAAAATTAAGCACAGTGGAAGAATTTCA
TTCTGTTCTCAGAATTCCTGGATTATGCCTGGCACCATTAAAGAAAATATCATCTTTGGT
GTTTCCTATGATGAATATAGATACAGAAGCGTCATCAAAGCATGCCAACTAGAAGAAGAC
ATCTCCAAGTTTGCAGAGAAAGACAATATAGTTCTTGGAGAAGGTGGAATCACACTGAGT
GGAGGTCAACGAGCAAGAATTTCTTTAGCAAGAGCAGTATACAAAGATGCTGATTTGTAT
TTATTAGACTCTCCTTTTGGATACCTAGATGTTTTAACAGAAAAAGAAATATTTGAAAGC
TGTGTCTGTAAACTGATGGCTAACAAAACTAGGATTTTGGTCACTTCTAAAATGGAACAT
TTAAAGAAAGCTGACAAAATATTAATTTTGCATGAAGGTAGCAGCTATTTTTATGGGACA
TTTTCAGAACTCCAAAATCTACGTCCAGACTTTAGCTCAAAACTCATGGGATGATAA

Protein sequence predicted based on nucleotide sequence

IENSHVKKDDIWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSGKST
LLSAFLRLLNTEGEIQIDGVSWDSITLEQWRKAFGVIPQKVFIFSGTFRKNLDPNAAHSD
QEIWKVADEVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEP
SAHLDPVTYQIIRRTLKQAFADCTVILCEARIEAMLECDQFLVIEENKVRQYDSIQKLLN
ERSLFRQAISPSDRPYEIKFTKTGERVEIERETLVTMENVTAFWEEGGTPVLKDINFKIE
RGQLLAVAGSTGAGKTSLLMMIMGELEPSEGKIKHSGRISFCSQNSWIMPGTIKENIIFG
VSYDEYRYRSVIKACQLEEDISKFAEKDNIVLGEGGITLSGGQRARISLARAVYKDADLY
LLDSPFGYLDVLTEKEIFESCVCKLMANKTRILVTSKMEHLKKADKILILHEGSSYFYGT
FSELQNLRPDFSSKLMG**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 1193
End on protein reference sequence 646
Mutations:


ins@1446,22

Discrepancy:
None
Others:
Termini 1193-646
Domain NBD2 linked to NBD1
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
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Recommended expression in:
Not Applicable