CFTR (Homo sapiens) in pSGX5

Explanation of Terms

Gene:
Gene Symbol:  CFTR
Gene Name:  cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
Original Clone ID:
5832c337CLt1pi1
Keyword:
Partial Protein, NBD2 domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSGX5
Format: 
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD2 domain and corresponds to region 1201-1446 on full length protein NP_000483.3.
Comments:
N-terminal 6-His-tag/SMT. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
No / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
HsCD00287361
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Insert sequence: 1107nts
Open reading frame: 1 to 1107

ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCAT
ATGGCTAGCATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAA
GTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTC
AAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGT
AAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGACC
CCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGT
GGATCAGATGACATCTGGCCCTCAGGGGGCCAAATGACTGTCAAAGATCTCACAGCAAAA
TACACAGAAGGTGGAAATGCCATATTAGAGAACATTTCCTTCTCAATAAGTCCTGGCCAG
AGGGTGGGCCTCTTGGGAAGAACTGGATCAGGGAAGAGTACTTTGTTATCAGCTTTTTTG
AGACTACTGAACACTGAAGGAGAAATCCAGATCGATGGTGTGTCTTGGGATTCAATAACT
TTGCAACAGTGGAGGAAAGCCTTTGGAGTGATACCACAGAAAGTATTTATTTTTTCTGGA
ACATTTAGAAAAAACTTGGATCCCTATGAACAGTGGAGTGACCAAGAAATATGGAAAGTT
GCAGATGAGGTTGGGCTCAGATCTGTGATAGAACAGTTTCCTGGGAAGCTTGACTTTGTC
CTTGTGGATGGGGGCTGTGTCCTAAGCCATGGCCACAAGCAGTTGATGTGCTTGGCTAGA
TCTGTTCTCAGTAAGGCGAAGATCTTGCTGCTTGATGAACCCAGTGCTCATTTGGATCCA
GTAACATACCAAATAATTAGAAGAACTCTAAAACAAGCATTTGCTGATTGCACAGTAATT
CTCTGTGAACACAGGATAGAAGCAATGCTGGAATGCCAACAATTTTTGGTCATAGAAGAG
AACAAAGTGCGGCAGTACGATTCCATCCAGAAACTGCTGAACGAGAGGAGCCTCTTCCGG
CAAGCCATCAGCCCCTCCGACAGGTAA

Protein sequence predicted based on nucleotide sequence

MGSSHHHHHHSSGLVPRGSHMASMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFF
KIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG
GSDDIWPSGGQMTVKDLTAKYTEGGNAILENISFSISPGQRVGLLGRTGSGKSTLLSAFL
RLLNTEGEIQIDGVSWDSITLQQWRKAFGVIPQKVFIFSGTFRKNLDPYEQWSDQEIWKV
ADEVGLRSVIEQFPGKLDFVLVDGGCVLSHGHKQLMCLARSVLSKAKILLLDEPSAHLDP
VTYQIIRRTLKQAFADCTVILCEHRIEAMLECQQFLVIEENKVRQYDSIQKLLNERSLFR
QAISPSDR*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 1201
End on protein reference sequence 1446
Mutations:
None
Discrepancy:
None
Others:
Termini 1201-1446
Domain NBD2
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Tested
SolubleProtein: Not_Tested
ProteinPurified: Protein_Purified
Find experimental protocols in 5832c
Recommended expression in:
Not Applicable