Gene:
Gene Symbol:
Cftr
Gene Name:
cystic fibrosis transmembrane conductance regulator
Original Clone ID:
5832f5CLt1p1
Keyword:
Partial Protein, NBD2 domain
Species:
Mus musculus
Type:
cDNA
Vector Name:
pSGX5
Format:
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD2 domain and corresponds to region 1166-1447 on full length protein NP_066388.1.
Comments:
N-terminal 6-His-tag/SMT. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
No / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
MmCD00287501
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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Special MTA: PSI/Smt3/SUMO
Insert sequence: 1215nts
Open reading frame: 1 to 1215
ATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCAT
ATGGCTAGCATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAA
GTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTC
AAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGT
AAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGACC
CCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGT
GGATCAACAGAAGAAAGTATGTACACACAGATAATTAAAGAACTACCTAGAGAAGGATCA
TCTGACGTTTTAGTCATTAAGAATGAGCATGTGAAGAAAAGTGATATCTGGCCCTCTGGA
GGCGAAATGGTTGTCAAAGACCTTACTGTGAAATACATGGATGATGGAAATGCCGTATTA
GAGAACATTTCTTTTTCAATAAGTCCTGGACAGAGGGTGGGGCTCTTAGGAAGAACTGGA
TCAGGAAAAAGTACTTTGCTTTCAGCATTTTTACGAATGTTGAACATTAAAGGTGATATA
GAGATTGATGGTGTCTCATGGAATTCAGTGACCTTACAAGAATGGAGGAAAGCTTTCGGA
GTGATAACACAGAAAGTATTTATCTTTTCTGGAACATTCAGACAAAACCTGGATCCCAAT
GGAAAATGGAAAGATGAAGAAATATGGAAAGTTGCAGATGAGGTTGGACTCAAGTCTGTA
ATAGAGCAGTTTCCTGGACAGCTCAACTTTACCCTTGTGGATGGGGGTTATGTGCTAAGC
CATGGCCATAAGCAATTAATGTGCTTGGCCCGATCAGTTCTCAGTAAGGCCAAGATCATA
CTGCTTGATGAGCCCAGTGCCCATCTAGACCCCATAACATACCAAGTCATTCGACGAGTT
CTAAAACAAGCCTTCGCTGGTTGCACAGTCATCCTCTGTGAACACAGGATAGAAGCGATG
TTGGATTGCCAGCGATTTTTGGTCATAGAAGAGAGCAATGTCTGGCAGTACGACTCCCTT
CAGGCACTTCTGAGTGAGAAGAGTATCTTCCAGCAGGCCATTAGCTCCTCGGAAAAGATG
AGGTTCTTCCAGTAA
Protein sequence predicted based on nucleotide sequence
MGSSHHHHHHSSGLVPRGSHMASMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFF KIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG GSTEESMYTQIIKELPREGSSDVLVIKNEHVKKSDIWPSGGEMVVKDLTVKYMDDGNAVL ENISFSISPGQRVGLLGRTGSGKSTLLSAFLRMLNIKGDIEIDGVSWNSVTLQEWRKAFG VITQKVFIFSGTFRQNLDPNGKWKDEEIWKVADEVGLKSVIEQFPGQLNFTLVDGGYVLS HGHKQLMCLARSVLSKAKIILLDEPSAHLDPITYQVIRRVLKQAFAGCTVILCEHRIEAM LDCQRFLVIEESNVWQYDSLQALLSEKSIFQQAISSSEKMRFFQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence
1166
End on protein reference sequence
1447
Mutations:
None
Discrepancy:
None
Others:
Termini
1166-1447
Domain NBD2
Domain NBD2
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Tested
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
Find experimental protocols in 5832f
Recommended expression in:
Not Applicable
Vector Name:
pSGX5
Synonyms:
pSGX5
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, ULP-1 cleavage site; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen. Smt3 fusion at N-terminus. Clone using BamHI and either HindIII, XhoI or NotI
Size (bp):
5633
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
start codon
5071
5073
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
primer site
T7
T7 sequencing primers
4985
5000
promoter
T7
T7 promoter
4984
5003
protease cleavage site
ULP site
ULP cleavage site cds
5242
5242
repressor protein gene
LacI
LacI gene
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
Smt3
N-terminal Smt3 tag
5140
5242
tag
His
6xHis tag
5083
5100
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 termination sequence
5562
5608
