Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol:  LOC100136364
Gene Name:  cystic fibrosis transmembrane conductance regulator I
Original Clone ID:
5806a39BSt2p1
Keyword:
Partial Protein, NBD1 domain
Species:
Salmo salar
Type:
cDNA
Vector Name:
pSGX4
Format: 
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD1 domain and corresponds to region 394-662 on full length protein AAK07405.1.
Comments:
N-terminal 6-His and Smt3, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
No / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
SsCD00287506
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Insert sequence: 810nts
Open reading frame: 1 to 810

GAACTCGTGAACGTGACGGCCAGCTGGGATGAGGGCATTGGCGAACTTTTCGAAAAAATC
AAACAGGAAAATAAAGCGAACGGCCAGCCAAACGGCGACGCGGGTCTGTTTTTTACCAAC
TTGTACGTCACCCCGGTGCTGAAAAATATTAGCCTTTACCTCGAGAAAGGGCAGATGCTG
GCGGTGGCAGGGTCAACGGGCAGCGGCAAATCCTCCTTACTGATGATGATTCTGGGTGAG
CTTGTGCCTTCCGAGGGCAAGATTCGTCATTCGGGCCGGATCAGCTTTTCCCCGCAGACT
TCGTGGATTATGCCAGGCACCATTCGCGATAACATCCTTTTTGGTCTTACGTATGATGAA
TTTCGTTACACCAGTGTCATTAAGGCCTGCCAGCTGGAAGAGGATCTGGCCTTACTGCCG
GAAAAAGACAAGACCCCGCTTATGGAAGGTGGCGTCACGTTGAGCGGCGGTCAACGCGCG
CGCATCGGTCTCGCTCGTGCTGTGTACAAAGACGCAGACCTGTATCTGCTCGACAGTCCA
TTCACGCATCTGGATATTGTTACCGAAAAGGAAATCTTTGAACGCTGTCTGTGCAAACTC
CTTTCTAGCAAAACGCGTATCGTCGTCACCTCGAAACTGGAACACCTGAAACGCGCGGAC
AAGATCCTGTTACTGCACAACGGGGTCTGCTATTTTTACGGTTCTTTCTCGGAACTGCAG
GCCAAACGCCCGGATTTTTCTTCATTACTGTTGGGGTTAGAAGCCTACGACAACATCAAA
GCAGAACGCCGTTCATCCATTTTGACCTAA

Protein sequence predicted based on nucleotide sequence

ELVNVTASWDEGIGELFEKIKQENKANGQPNGDAGLFFTNLYVTPVLKNISLYLEKGQML
AVAGSTGSGKSSLLMMILGELVPSEGKIRHSGRISFSPQTSWIMPGTIRDNILFGLTYDE
FRYTSVIKACQLEEDLALLPEKDKTPLMEGGVTLSGGQRARIGLARAVYKDADLYLLDSP
FTHLDIVTEKEIFERCLCKLLSSKTRIVVTSKLEHLKRADKILLLHNGVCYFYGSFSELQ
AKRPDFSSLLLGLEAYDNIKAERRSSILT*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 394
End on protein reference sequence 662
Mutations:
None
Discrepancy:
None
Others:
Termini 394-662
Domain NBD1
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 5806a
Recommended expression in:
Not Applicable