Cftr (M. musculus )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  Cftr
Gene Name:  cystic fibrosis transmembrane conductance regulator
Original Clone ID:
5784f3BCt2p1
Keyword:
Partial Protein, NBD1 domain
Species:
Mus musculus
Type:
cDNA
Vector Name:
pSGX3
Format: 
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD1 domain with other intended mutations and corresponds to region 388-673 on full length protein NP_066388.1.
Comments:
C-terminal 6-His, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
Yes / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
MmCD00287533
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 911nts
Open reading frame: 1 to 911

ATGACCACAGGCATAATCATGGAAAATGTAACAGCATTTTGGGAGGAGGGATTTGGGGAA
TTACTGGAGAAAGTACAACAAAGCAATGGTGACAGAAAACATTCCAGTGATGAGAACAAT
GTCAGTTTCAGTCATCTCTGCCTTGTGGGAAATCCTGTGCTGAAAAACATCAATTTGAAT
ATAGAGAAAGGAGAGATGTTGGCTATTACTGGATCTACTGGATCAGGAAAGACATCACTC
CTGATGTTGATTTTGGGAGAACTGGAAGCTTCAGAGGGAATTATTAAGCACAGTGGAAGA
GTTTCATTCTGCTCTCAATTTTCTTGGATTATGCCGGGTACTATCAAAGAAAATATCATC
GGTGGTGTTTCCTATGATGAGTACAGATATAAGAGTGTTGTCAAAGCTTGCCAACTACAG
CAGGACATCACCAAGTTTGCAGAACAAGACAACACAGTTCTTGGAGAAGGTGGAGTCACA
CTGAGTGGAGGTCAGCGTGCAAGGATTTCTTTAGCAAGAGCAGTATATAAAGATGCTGAT
TTGTACCTATTAGATTCCCCTTTTGGGTATCTAGATGTTTTTACTGGAGAACAAGTATTT
GAAAGCTGTGTTTGTAAATTGATGGCCAACAAAACTAGGATTTTGGTTACATCTAAAATG
GAACACTTAAGGAAAGCTGACAAAATACTAATTTTGCATCAGGGCAGTAGCTATTTTTAT
GGGACATTTTCTGAGCTACAAAGTCTACGTCCAGACTTCAGTTCGAAACTCATGGGGTAT
GATACTTTTGACCAGTTTACTGAGGAAAGAAGAAGTTCAATTCTAACTGAGACCTTACGC
AGGTTCTCAGTAGACGATGAAGGGCATCATCACCATCACCACTGAAAGGGCATCATCACC
ATCACCACTGA

Protein sequence predicted based on nucleotide sequence

MTTGIIMENVTAFWEEGFGELLEKVQQSNGDRKHSSDENNVSFSHLCLVGNPVLKNINLN
IEKGEMLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENII
GGVSYDEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDAD
LYLLDSPFGYLDVFTGEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFY
GTFSELQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFSVDDEGHHHHHH*KGIIT
ITT

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 388
End on protein reference sequence 673
Mutations:
F408G; Q410E; A412V; L462S; E583G; T623S

F508G, E583G

Discrepancy:
None
Others:
Termini 388-673
Domain NBD1
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in 5784f
Recommended expression in:
Not Applicable