Cftr (Mus musculus) in pSGX4

Explanation of Terms

Gene:
Gene Symbol:  Cftr
Gene Name:  cystic fibrosis transmembrane conductance regulator
Original Clone ID:
5811f26BSt3p1
Keyword:
Partial Protein, NBD1 domain
Species:
Mus musculus
Type:
cDNA
Vector Name:
pSGX4
Format: 
FUSION
Source:
SGX Pharmaceuticals, Inc.
Description:
This clone encodes the NBD1 domain with other intended mutations and corresponds to region 393-670 on full length protein NP_066388.1.
Comments:
N-terminal 6-His and Smt3, cleavable, TOPO. Insert sequence of this clone was provided by clone submitter and was not fully sequence verified.
Mutation / Discrepancy:
Yes / Unknown
Publications:
None
Authors:
SGX Pharmaceuticals, Inc.
MmCD00287561
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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Insert sequence: 837nts
Open reading frame: 1 to 837

ATCATGGAAAATGTAACAGCATTTTGGGAGGAGGGATTTGGGGAATTACTGGAGAAAGTA
CAACAAAGCAATGGTGACAGAAAACATTCCAGTGATGAGAACAATGTCAGTTTCAGTCAT
CTCTGCCTTGTGGGAAATCCTGTGCTGAAAAACATCAATTTGAATATAGAGAAAGGAGAG
ATGTTGGCTATTACTGGATCTACTGGATCAGGAAAGACATCACTCCTGATGTTGATTTTG
GGAGAACTGGAAGCTTCAGAGGGAATTATTAAGCACAGTGGAAGAGTTTCATTCTGCTCT
CAATTTTCTTGGATTATGCCGGGTACTATCAAAGAAAATATCATCTTTGGTGTTTCCTAT
GATGAGTACAGATATAAGAGTGTTGTCAAAGCTTGCCAACTACAGCAGGACATCACCAAG
TTTGCAGAACAAGACAACACAGTTCTTGGAGAAGGTGGAGTCACACTGAGTGGAGGTCAG
CGTGCAAGGATTTCTTTAGCAAGAGCAGTATATAAAGATGCTGATTTGTACCTATTAGAT
TCCCCTTTTGGGTATCTAGATGTTTTTACTGGAGAACAAGTATTTGAAAGCTGTGTTTGT
AAATTGATGGCCAACAAAACTAGGATTTTGGTTACATCTAAAATGGAACACTTAAGGAAA
GCTGACAAAATACTAATTTTGCATCAGGGCAGTAGCTATTTTTATGGGACATTTTCTGAG
CTACAAAGTCTACGTCCAGACTTCAGTTCGAAACTCATGGGGTATGATACTTTTGACCAG
TTTACTGAGGAAAGAAGAAGTTCAATTCTAACTGAGACCTTACGCAGGTTCTCATAA

Protein sequence predicted based on nucleotide sequence

IMENVTAFWEEGFGELLEKVQQSNGDRKHSSDENNVSFSHLCLVGNPVLKNINLNIEKGE
MLAITGSTGSGKTSLLMLILGELEASEGIIKHSGRVSFCSQFSWIMPGTIKENIIFGVSY
DEYRYKSVVKACQLQQDITKFAEQDNTVLGEGGVTLSGGQRARISLARAVYKDADLYLLD
SPFGYLDVFTGEQVFESCVCKLMANKTRILVTSKMEHLRKADKILILHQGSSYFYGTFSE
LQSLRPDFSSKLMGYDTFDQFTEERRSSILTETLRRFS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing of cDNA insert
Reference Sequence Annotations:
Start on protein reference sequence 393
End on protein reference sequence 670
Mutations:
E583G

Q410E; A412V; L462S; E583G; T623S; S639D

Discrepancy:
None
Others:
Termini 393-670
Domain NBD1
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 5811f
Recommended expression in:
Not Applicable