DNASU Plasmid | ATP6V1E2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ATP6V1E2

Gene Name: ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2

Original Clone ID:

1570

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287653

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 681nts

Open reading frame: 1 to 681

Reference Sequence Alignment

ATGGCCCTGCGTGATGTCGATGTGAAAAAGCAGATTAAGCACATGATGGCTTTCATTGAG
CAGGAAGCCAATGAGAAAGCAGAGGAAATCGATGCCAAGGCTGAGGAAGAGTTTAACATT
GAGAAAGGACGCCTCGTGCAAACCCAACGACTGAAGATTATGGAGTATTATGAGAAAAAG
GAGAAGCAGATAGAGCAGCAGAAGAAAATCCTGATGTCCACCATGAGGAATCAGGCGAGG
CTGAAAGTCCTGAGAGCCCGAAATGACCTCATCTCAGATTTGCTCAGTGAGGCGAAGCTG
AGACTCAGCAGGATTGTGGAGGACCCAGAGGTCTACCAGGGGCTGCTGGATAAACTGGTG
CTCCAGGGTCTGCTCCGACTGCTGGAACCTGTGATGATTGTACGCTGCCGGCCACAAGAC
CTCCTCCTGGTGGAGGCTGCTGTACAAAAAGCCATCCCCGAGTACATGACAATTTCCCAG
AAACATGTGGAGGTCCAGATTGATAAAGAGGCATACCTGGCTGTGAATGCAGCTGGAGGT
GTGGAGGTCTACAGTGGCAATCAGAGAATAAAGGTTTCAAATACCTTGGAAAGCCGACTG
GATCTCTCAGCCAAGCAAAAGATGCCAGAAATACGAATGGCCTTGTTTGGTGCTAACACC
AACAGAAAGTTCTTTATATAC

Protein sequence predicted based on nucleotide sequence

MALRDVDVKKQIKHMMAFIEQEANEKAEEIDAKAEEEFNIEKGRLVQTQRLKIMEYYEKK
EKQIEQQKKILMSTMRNQARLKVLRARNDLISDLLSEAKLRLSRIVEDPEVYQGLLDKLV
LQGLLRLLEPVMIVRCRPQDLLLVEAAVQKAIPEYMTISQKHVEVQIDKEAYLAVNAAGG
VEVYSGNQRIKVSNTLESRLDLSAKQKMPEIRMALFGANTNRKFFIY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1056

Start on reference sequence 376

Mutations:

None

Discrepancy:

a10c,S4R

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ATP6V1E2

Symbol Nomenclature :

ATP6V1E2

SYNONYM :

ATP6E1; ATP6EL2; ATP6V1EL2; VMA4

Designation :

V-ATPase subunit E 2|V-type proton ATPase subunit E 2|vacuolar proton pump subunit E 2|vacuolar-type proton-translocating ATPase subunit E1

Full Nomenclature :

ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E2

GENEID :

90423

HGNC :

18125

Vega :

OTTHUMG00000128819

Target GenBank:

BC008981.1

Reference Sequence Alignment

  

HsCD00287653        ATGGCCCTGCGTGATGTCGATGTGAAAAAGCAGATTAAGCACATGATGGCTTTCATTGAG
XM_005264634.1      ATGGCCCTGAGTGATGTCGATGTGAAAAAGCAGATTAAGCACATGATGGCTTTCATTGAG
NM_080653.3         ATGGCCCTGAGTGATGTCGATGTGAAAAAGCAGATTAAGCACATGATGGCTTTCATTGAG
XM_005264633.1      ATGGCCCTGAGTGATGTCGATGTGAAAAAGCAGATTAAGCACATGATGGCTTTCATTGAG
                    *********.**************************************************

HsCD00287653        CAGGAAGCCAATGAGAAAGCAGAGGAAATCGATGCCAAGGCTGAGGAAGAGTTTAACATT
XM_005264634.1      CAGGAAGCCAATGAGAAAGCAGAGGAAATCGATGCCAAGGCTGAGGAAGAGTTTAACATT
NM_080653.3         CAGGAAGCCAATGAGAAAGCAGAGGAAATCGATGCCAAGGCTGAGGAAGAGTTTAACATT
XM_005264633.1      CAGGAAGCCAATGAGAAAGCAGAGGAAATCGATGCCAAGGCTGAGGAAGAGTTTAACATT
                    ************************************************************

HsCD00287653        GAGAAAGGACGCCTCGTGCAAACCCAACGACTGAAGATTATGGAGTATTATGAGAAAAAG
XM_005264634.1      GAGAAAGGACGCCTCGTGCAAACCCAACGACTGAAGATTATGGAGTATTATGAGAAAAAG
NM_080653.3         GAGAAAGGACGCCTCGTGCAAACCCAACGACTGAAGATTATGGAGTATTATGAGAAAAAG
XM_005264633.1      GAGAAAGGACGCCTCGTGCAAACCCAACGACTGAAGATTATGGAGTATTATGAGAAAAAG
                    ************************************************************

HsCD00287653        GAGAAGCAGATAGAGCAGCAGAAGAAAATCCTGATGTCCACCATGAGGAATCAGGCGAGG
XM_005264634.1      GAGAAGCAGATAGAGCAGCAGAAGAAAATCCTGATGTCCACCATGAGGAATCAGGCGAGG
NM_080653.3         GAGAAGCAGATAGAGCAGCAGAAGAAAATCCTGATGTCCACCATGAGGAATCAGGCGAGG
XM_005264633.1      GAGAAGCAGATAGAGCAGCAGAAGAAAATCCTGATGTCCACCATGAGGAATCAGGCGAGG
                    ************************************************************

HsCD00287653        CTGAAAGTCCTGAGAGCCCGAAATGACCTCATCTCAGATTTGCTCAGTGAGGCGAAGCTG
XM_005264634.1      CTGAAAGTCCTGAGAGCCCGAAATGACCTCATCTCAGATTTGCTCAGTGAGGCGAAGCTG
NM_080653.3         CTGAAAGTCCTGAGAGCCCGAAATGACCTCATCTCAGATTTGCTCAGTGAGGCGAAGCTG
XM_005264633.1      CTGAAAGTCCTGAGAGCCCGAAATGACCTCATCTCAGATTTGCTCAGTGAGGCGAAGCTG
                    ************************************************************

HsCD00287653        AGACTCAGCAGGATTGTGGAGGACCCAGAGGTCTACCAGGGGCTGCTGGATAAACTGGTG
XM_005264634.1      AGACTCAGCAGGATTGTGGAGGACCCAGAGGTCTACCAGGGGCTGCTGGATAAACTGGTG
NM_080653.3         AGACTCAGCAGGATTGTGGAGGACCCAGAGGTCTACCAGGGGCTGCTGGATAAACTGGTG
XM_005264633.1      AGACTCAGCAGGATTGTGGAGGACCCAGAGGTCTACCAGGGGCTGCTGGATAAACTGGTG
                    ************************************************************

HsCD00287653        CTCCAGGGTCTGCTCCGACTGCTGGAACCTGTGATGATTGTACGCTGCCGGCCACAAGAC
XM_005264634.1      CTCCAGGGTCTGCTCCGACTGCTGGAACCTGTGATGATTGTACGCTGCCGGCCACAAGAC
NM_080653.3         CTCCAGGGTCTGCTCCGACTGCTGGAACCTGTGATGATTGTACGCTGCCGGCCACAAGAC
XM_005264633.1      CTCCAGGGTCTGCTCCGACTGCTGGAACCTGTGATGATTGTACGCTGCCGGCCACAAGAC
                    ************************************************************

HsCD00287653        CTCCTCCTGGTGGAGGCTGCTGTACAAAAAGCCATCCCCGAGTACATGACAATTTCCCAG
XM_005264634.1      CTCCTCCTGGTGGAGGCTGCTGTACAAAAAGCCATCCCCGAGTACATGACAATTTCCCAG
NM_080653.3         CTCCTCCTGGTGGAGGCTGCTGTACAAAAAGCCATCCCCGAGTACATGACAATTTCCCAG
XM_005264633.1      CTCCTCCTGGTGGAGGCTGCTGTACAAAAAGCCATCCCCGAGTACATGACAATTTCCCAG
                    ************************************************************

HsCD00287653        AAACATGTGGAGGTCCAGATTGATAAAGAGGCATACCTGGCTGTGAATGCAGCTGGAGGT
XM_005264634.1      AAACATGTGGAGGTCCAGATTGATAAAGAGGCATACCTGGCTGTGAATGCAGCTGGAGGT
NM_080653.3         AAACATGTGGAGGTCCAGATTGATAAAGAGGCATACCTGGCTGTGAATGCAGCTGGAGGT
XM_005264633.1      AAACATGTGGAGGTCCAGATTGATAAAGAGGCATACCTGGCTGTGAATGCAGCTGGAGGT
                    ************************************************************

HsCD00287653        GTGGAGGTCTACAGTGGCAATCAGAGAATAAAGGTTTCAAATACCTTGGAAAGCCGACTG
XM_005264634.1      GTGGAGGTCTACAGTGGCAATCAGAGAATAAAGGTTTCAAATACCTTGGAAAGCCGACTG
NM_080653.3         GTGGAGGTCTACAGTGGCAATCAGAGAATAAAGGTTTCAAATACCTTGGAAAGCCGACTG
XM_005264633.1      GTGGAGGTCTACAGTGGCAATCAGAGAATAAAGGTTTCAAATACCTTGGAAAGCCGACTG
                    ************************************************************

HsCD00287653        GATCTCTCAGCCAAGCAAAAGATGCCAGAAATACGAATGGCCTTGTTTGGTGCTAACACC
XM_005264634.1      GATCTCTCAGCCAAGCAAAAGATGCCAGAAATACGAATGGCCTTGTTTGGTGCTAACACC
NM_080653.3         GATCTCTCAGCCAAGCAAAAGATGCCAGAAATACGAATGGCCTTGTTTGGTGCTAACACC
XM_005264633.1      GATCTCTCAGCCAAGCAAAAGATGCCAGAAATACGAATGGCCTTGTTTGGTGCTAACACC
                    ************************************************************

HsCD00287653        AACAGAAAGTTCTTTATATAC
XM_005264634.1      AACAGAAAGTTCTTTATATAA
NM_080653.3         AACAGAAAGTTCTTTATATAA
XM_005264633.1      AACAGAAAGTTCTTTATATAA
                    ********************.

Pathways

Reactome :

Disease

Reactome :

Insulin receptor recycling

Reactome :

Iron uptake and transport

Reactome :

Latent infection of Homo sapiens with Mycobacterium tuberculosis

Reactome :

Phagosomal maturation (early endosomal stage)

Reactome :

Signal Transduction

Reactome :

Signaling by Insulin receptor

Reactome :

Transferrin endocytosis and recycling

Reactome :

Transmembrane transport of small molecules

Protein

UniProt :

Q96A05

HPRD :

16525

Clone

Cloning Information :

470890

HIP Master Clone ID :

393808

Original Clone ID :

1570

Organism

Species Specific ID:

90423

Genome

Chromosome :

Map Location :

2p21

Ensembl :

ENSG00000250565

Reagents

Labome :

ATP6V1E2-antibody