DNASU Plasmid | DECR2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: DECR2

Gene Name: 2,4-dienoyl CoA reductase 2, peroxisomal

Original Clone ID:

2146

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287688

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 879nts

Open reading frame: 1 to 879

Reference Sequence Alignment

ATGGCCCAGCCGCCGCCCGACGTGGAGGGGGACGACTGTCTCCCCGCGTACCGCCACCTC
TTCTGCCCGGACCTGCTGCGGGACAAAGTGGCCTTCATCACAGGAGGCGGCTCTGGGATT
GGGTTCCGGATTGCTGAGATTTTCATGCGGCACGGCTGCCATACGGTGATTGCCAGTAGG
AGCCTGCCGCGAGTGCTGACGGCCGCCAGGAAGCTGGCTGGGGCCACCGGCCGGCGCTGC
CTCCCTCTCTCTATGGACGTCCGAGCGCCCCCAGCTGTCATGGCCGCCGTGGACCAGGCT
CTGAAGGAGTTTGGCAGAATCGACATTCTCATTAACTGTGCGGCCGGGAACTTCCTGTGC
CCCGCTGGCGCCTTGTCCTTCAACGCCTTCAAGACCGTGATGGACATCGATACCAGCGGC
ACCTTCAATGTGTCTCGTGTGCTCTATGAGAAGTTCTTCCGGGACCACGGAGGGGTGATC
GTGAACATCACTGCCACCCTGGGGAACCGGGGGCAGGCGCTCCAGGTGCATGCAGGCTCC
GCCAAGGCCGCTGTGGACGCGATGACGCGGCACTTGGCTGTGGAGTGGGGTCCCCAAAAC
ATCCGCGTCAACAGCCTCGCCCCTGGCCCCATCAGTGGCACAGAGGGGCTCCGGCGACTG
GGTGGCCCTCAGGCCAGCCTGAGCACCAAGGTCACTGCCAGCCCGCTGCAGAGGCTGGGG
AACAAGACCGAGATCGCCCACAGCGTGCTCTACCTGGCCAGCCCTCTGGCTTCCTACGTG
ACGGGGGCCGTGCTGGTGGCCGATGGCGGGGCATGGTTGACGTTCCCAAACGGTGTCAAA
GGGCTGCCGGATTTCGCATCCTTCTCTGCTAAGCTCTAC

Protein sequence predicted based on nucleotide sequence

MAQPPPDVEGDDCLPAYRHLFCPDLLRDKVAFITGGGSGIGFRIAEIFMRHGCHTVIASR
SLPRVLTAARKLAGATGRRCLPLSMDVRAPPAVMAAVDQALKEFGRIDILINCAAGNFLC
PAGALSFNAFKTVMDIDTSGTFNVSRVLYEKFFRDHGGVIVNITATLGNRGQALQVHAGS
AKAAVDAMTRHLAVEWGPQNIRVNSLAPGPISGTEGLRRLGGPQASLSTKVTASPLQRLG
NKTEIAHSVLYLASPLASYVTGAVLVADGGAWLTFPNGVKGLPDFASFSAKLY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 93

End on reference sequence 971

Mutations:

None

Discrepancy:

a672g

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

DECR2

Symbol Nomenclature :

DECR2

SYNONYM :

PDCR; SDR17C1

Designation :

2,4-dienoyl-CoA reductase 2|peroxisomal 2,4-dienoyl-CoA reductase|short chain dehydrogenase/reductase family 17C, member 1

Full Nomenclature :

2,4-dienoyl CoA reductase 2, peroxisomal

GENEID :

26063

Locus Tag :

AL023881.1

HGNC :

2754

Vega :

OTTHUMG00000047846

Target GenBank:

BC010740.1

Reference Sequence Alignment

  

HsCD00287688      ATGGCCCAGCCGCCGCCCGACGTGGAGGGGGACGACTGTCTCCCCGCGTACCGCCACCTC
NM_020664.3       ATGGCCCAGCCGCCGCCCGACGTGGAGGGGGACGACTGTCTCCCCGCGTACCGCCACCTC
                  ************************************************************

HsCD00287688      TTCTGCCCGGACCTGCTGCGGGACAAAGTGGCCTTCATCACAGGAGGCGGCTCTGGGATT
NM_020664.3       TTCTGCCCGGACCTGCTGCGGGACAAAGTGGCCTTCATCACAGGAGGCGGCTCTGGGATT
                  ************************************************************

HsCD00287688      GGGTTCCGGATTGCTGAGATTTTCATGCGGCACGGCTGCCATACGGTGATTGCCAGTAGG
NM_020664.3       GGGTTCCGGATTGCTGAGATTTTCATGCGGCACGGCTGCCATACGGTGATTGCCAGTAGG
                  ************************************************************

HsCD00287688      AGCCTGCCGCGAGTGCTGACGGCCGCCAGGAAGCTGGCTGGGGCCACCGGCCGGCGCTGC
NM_020664.3       AGCCTGCCGCGAGTGCTGACGGCCGCCAGGAAGCTGGCTGGGGCCACCGGCCGGCGCTGC
                  ************************************************************

HsCD00287688      CTCCCTCTCTCTATGGACGTCCGAGCGCCCCCAGCTGTCATGGCCGCCGTGGACCAGGCT
NM_020664.3       CTCCCTCTCTCTATGGACGTCCGAGCGCCCCCAGCTGTCATGGCCGCCGTGGACCAGGCT
                  ************************************************************

HsCD00287688      CTGAAGGAGTTTGGCAGAATCGACATTCTCATTAACTGTGCGGCCGGGAACTTCCTGTGC
NM_020664.3       CTGAAGGAGTTTGGCAGAATCGACATTCTCATTAACTGTGCGGCCGGGAACTTCCTGTGC
                  ************************************************************

HsCD00287688      CCCGCTGGCGCCTTGTCCTTCAACGCCTTCAAGACCGTGATGGACATCGATACCAGCGGC
NM_020664.3       CCCGCTGGCGCCTTGTCCTTCAACGCCTTCAAGACCGTGATGGACATCGATACCAGCGGC
                  ************************************************************

HsCD00287688      ACCTTCAATGTGTCTCGTGTGCTCTATGAGAAGTTCTTCCGGGACCACGGAGGGGTGATC
NM_020664.3       ACCTTCAATGTGTCTCGTGTGCTCTATGAGAAGTTCTTCCGGGACCACGGAGGGGTGATC
                  ************************************************************

HsCD00287688      GTGAACATCACTGCCACCCTGGGGAACCGGGGGCAGGCGCTCCAGGTGCATGCAGGCTCC
NM_020664.3       GTGAACATCACTGCCACCCTGGGGAACCGGGGGCAGGCGCTCCAGGTGCATGCAGGCTCC
                  ************************************************************

HsCD00287688      GCCAAGGCCGCTGTGGACGCGATGACGCGGCACTTGGCTGTGGAGTGGGGTCCCCAAAAC
NM_020664.3       GCCAAGGCCGCTGTGGACGCGATGACGCGGCACTTGGCTGTGGAGTGGGGTCCCCAAAAC
                  ************************************************************

HsCD00287688      ATCCGCGTCAACAGCCTCGCCCCTGGCCCCATCAGTGGCACAGAGGGGCTCCGGCGACTG
NM_020664.3       ATCCGCGTCAACAGCCTCGCCCCTGGCCCCATCAGTGGCACAGAGGGGCTCCGGCGACTG
                  ************************************************************

HsCD00287688      GGTGGCCCTCAGGCCAGCCTGAGCACCAAGGTCACTGCCAGCCCGCTGCAGAGGCTGGGG
NM_020664.3       GGTGGCCCTCAGGCCAGCCTGAGCACCAAGGTCACTGCCAGCCCGCTGCAGAGGCTGGGG
                  ************************************************************

HsCD00287688      AACAAGACCGAGATCGCCCACAGCGTGCTCTACCTGGCCAGCCCTCTGGCTTCCTACGTG
NM_020664.3       AACAAGACCGAGATCGCCCACAGCGTGCTCTACCTGGCCAGCCCTCTGGCTTCCTACGTG
                  ************************************************************

HsCD00287688      ACGGGGGCCGTGCTGGTGGCCGATGGCGGGGCATGGTTGACGTTCCCAAACGGTGTCAAA
NM_020664.3       ACGGGGGCCGTGCTGGTGGCCGATGGCGGGGCATGGTTGACGTTCCCAAACGGTGTCAAA
                  ************************************************************

HsCD00287688      GGGCTGCCGGATTTCGCATCCTTCTCTGCTAAGCTCTAC
NM_020664.3       GGGCTGCCGGATTTCGCATCCTTCTCTGCTAAGCTCTAG
                  **************************************