DNASU Plasmid | RBM11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RBM11

Gene Name: RNA binding motif protein 11

Original Clone ID:

2125

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287690

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 846nts

Open reading frame: 1 to 846

Reference Sequence Alignment

ATGTTCCCTGCTCAGGAGGAGGCCGACAGGACCGTGTTTGTTGGGAATTTAGAGGCCCGA
GTTCGGGAAGAGATTCTGTACGAGCTGTTCCTTCAGGCGGGGCCACTAACCAAAGTGACT
ATATGCAAAGACAGAGAAGGAAAGCCAAAGTCTTTTGGATTTGTCTGCTTTAAACACCCA
GAATCGGTGTCTTATGCCATAGCTTTGCTGAATGGAATTCGTTTATATGGAAGACCAATT
AACGTGCAGTATCGATTTGGGAGTTCTCGCTCTTCTGAACCAGCTAACCAAAGTTTTGAG
AGCTGTGTTAAGATAAATTCACACAACTACAGGAATGAAGAAATGTTGGTGGGCAGATCT
TCCTTTCCCATGCAGTATTTTCCAATTAATAATACTTCTTTACCTCAAGAATATTTTCTC
TTTCAGAAGATGCAGTGGCATGTGTATAATCCAGTGCTGCAGCTTCCTTACTATGAAATG
ACAGCTCCACTTCCTAATAGTGCATCCGTGTCTTCCTCACTGAATCATGTTCCAGATCTT
GAGGCTGGACCCAGCTCATATAAATGGACTCACCAACAACCAAGTGACTCTGACCTTTAT
CAGATGACAGCTCCACTTCCTAATAGTGCATCCGTGTCTTCCTCACTGAATCATGTTCCA
GATCTTGAGGCTGGACCCAGCTCATATAAATGGACTCACCAACAACCAAGTGACTCTGAC
CTTTATCAGATGAATAAACGAAAGAGACAAAAGCAAACAAGTGATAGTGATAGTAGCACA
GACAACAACAGAGGCAACGAATGTAGCCAAAAGTTCCGAAAGTCTAAGAAGAAGAAAAGA
TACTAC

Protein sequence predicted based on nucleotide sequence

MFPAQEEADRTVFVGNLEARVREEILYELFLQAGPLTKVTICKDREGKPKSFGFVCFKHP
ESVSYAIALLNGIRLYGRPINVQYRFGSSRSSEPANQSFESCVKINSHNYRNEEMLVGRS
SFPMQYFPINNTSLPQEYFLFQKMQWHVYNPVLQLPYYEMTAPLPNSASVSSSLNHVPDL
EAGPSSYKWTHQQPSDSDLYQMTAPLPNSASVSSSLNHVPDLEAGPSSYKWTHQQPSDSD
LYQMNKRKRQKQTSDSDSSTDNNRGNECSQKFRKSKKKKRYY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 10

End on reference sequence 855

Mutations:

None

Discrepancy:

ins@1,342

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

RBM11

Symbol Nomenclature :

RBM11

Designation :

RNA-binding motif protein 11|putative RNA-binding protein 11|splicing regulator RBM11

Full Nomenclature :

RNA binding motif protein 11

GENEID :

54033

HGNC :

9897

Vega :

OTTHUMG00000074263

Target GenBank:

BC030196.1

Reference Sequence Alignment

  

HsCD00287690        ATGTTCCCTGCTCAGGAGGAGGCCGACAGGACCGTGTTTGTTGGGAATTTAGAGGCCCGA
XM_005260996.1      ATGTTCCCTGCTCAGGAGGAGGCCGACAGGACCGTGTTTGTTGGGAATTTAGAGGCCCGA
NM_144770.3         ATGTTCCCTGCTCAGGAGGAGGCCGACAGGACCGTGTTTGTTGGGAATTTAGAGGCCCGA
                    ************************************************************

HsCD00287690        GTTCGGGAAGAGATTCTGTACGAGCTGTTCCTTCAGGCGGGGCCACTAACCAAAGTGACT
XM_005260996.1      GTTCGGGAAGAGATTCTGTACGAGCTGTTCCTTCAGGCGGGGCCACTAACCAAAGTGACT
NM_144770.3         GTTCGGGAAGAGATTCTGTACGAGCTGTTCCTTCAGGCGGGGCCACTAACCAAAGTGACT
                    ************************************************************

HsCD00287690        ATATGCAAAGACAGAGAAGGAAAGCCAAAGTCTTTTGGATTTGTCTGCTTTAAACACCCA
XM_005260996.1      ATATGCAAAGACAGAGAAGGAAAGCCAAAGTCTTTTGGATTTGTCTGCTTTAAACACCCA
NM_144770.3         ATATGCAAAGACAGAGAAGGAAAGCCAAAGTCTTTTGGATTTGTCTGCTTTAAACACCCA
                    ************************************************************

HsCD00287690        GAATCGGTGTCTTATGCCATAGCTTTGCTGAATGGAATTCGTTTATATGGAAGACCAATT
XM_005260996.1      GAATCGGTGTCTTATGCCATAGCTTTGCTGAATGGAATTCGTTTATATGGAAGACCAATT
NM_144770.3         GAATCGGTGTCTTATGCCATAGCTTTGCTGAATGGAATTCGTTTATATGGAAGACCAATT
                    ************************************************************

HsCD00287690        AACGTGCAGTATCGATTTGGGAGTTCTCGCTCTTCTGAACCAGCTAACCAAAGTTTTGAG
XM_005260996.1      AACGTGCAGTATCGATTTGGGAGTTCTCGCTCTTCTGAACCAGCTAACCAAAGTTTTGAG
NM_144770.3         AACGTGCAGTATCGATTTGGGAGTTCTCGCTCTTCTGAACCAGCTAACCAAAGTTTTGAG
                    ************************************************************

HsCD00287690        AGCTGTGTTAAGATAAATTCACACAACTACAGGAATGAAGAAATGTTGGTGGGCAGATCT
XM_005260996.1      AGCTGTGTTAAGATAAATTCACACAACTACAGGAATGAAGAAATGTTGGTGGGCAGATCT
NM_144770.3         AGCTGTGTTAAGATAAATTCACACAACTACAGGAATGAAGAAATGTTGGTGGGCAGATCT
                    ************************************************************

HsCD00287690        TCCTTTCCCATGCAGTATTTTCCAATTAATAATACTTCTTTACCTCAAGAATATTTTCTC
XM_005260996.1      TCCTTTCCCATGCAGTATTTTCCAATTAATAATACTTCTTTACCTCAAGAATATTTTCTC
NM_144770.3         TCCTTTCCCATGCAGTATTTTCCAATTAATAATACTTCTTTACCTCAAGAATATTTTCTC
                    ************************************************************

HsCD00287690        TTTCAGAAGATG---------------------CAGTGGCATGTGTATAATCCAGTGCTG
XM_005260996.1      TTTCAGAAGATGATGAATGTCTTGTTTTCACAGCAGTGGCATGTGTATAATCCAGTGCTG
NM_144770.3         TTTCAGAAGAT---------------------GCAGTGGCATGTGTATAATCCAGTGCTG
                    ***********                      ***************************

HsCD00287690        CAGCTTCCTTACTATGAAATGACAGCTCCACTTCCTAATAGTGCATCCGTGTCTTCCTCA
XM_005260996.1      CAGCTTCCTTACTATGAAATGACAGCTCCACTTCCTAATAGTGCATCCGTGTCTTCCTCA
NM_144770.3         CAGCTTCCTTACTATGAAATGACAGCTCCACTTCCTAATAGTGCATCCGTGTCTTCCTCA
                    ************************************************************

HsCD00287690        CTGAATCATGTTCCAGATCTTGAGGCTGGACCCAGCTCATATAAATGGACTCACCAACAA
XM_005260996.1      CTGAATCATGTTCCAGATCTTGAGGCTGGACCCAGCTCATATAAATGGACTCACCAACAA
NM_144770.3         CTGAATCATGTTCCAGATCTTGAGGCTGGACCCAGCTCATATAAATGGACTCACCAACAA
                    ************************************************************

HsCD00287690        CCAAGTGACTCTGACCTTTATCAGATGACAGCTCCACTTCCTAATAGTGCATCCGTGTCT
XM_005260996.1      CCAAGTGACTCTGACCTTTATCAGATGACAGCTCCACTTCCTAATAGTGCATCCGTGTCT
NM_144770.3         CCAAGTGACTCTGACCTTTATCAGATGACAGCTCCACTTCCTAATAGTGCATCCGTGTCT
                    ************************************************************

HsCD00287690        TCCTCACTGAATCATGTTCCAGATCTTGAGGCTGGACCCAGCTCATATAAATGGACTCAC
XM_005260996.1      TCCTCACTGAATCATGTTCCAGATCTTGAGGCTGGACCCAGCTCATATAAATGGACTCAC
NM_144770.3         TCCTCACTGAATCATGTTCCAGATCTTGAGGCTGGACCCAGCTCATATAAATGGACTCAC
                    ************************************************************

HsCD00287690        CAACAACCAAGTGACTCTGACCTTTATCAGATGAATAAACGAAAGAGACAAAAGCAAACA
XM_005260996.1      CAACAACCAAGTGACTCTGACCTTTATCAGATGAATAAACGAAAGAGACAAAAGCAAACA
NM_144770.3         CAACAACCAAGTGACTCTGACCTTTATCAGATGAATAAACGAAAGAGACAAAAGCAAACA
                    ************************************************************

HsCD00287690        AGTGATAGTGATAGTAGCACAGACAACAACAGAGGCAACGAATGTAGCCAAAAGTTCCGA
XM_005260996.1      AGTGATAGTGATAGTAGCACAGACAACAACAGAGGCAACGAATGTAGCCAAAAGTTCCGA
NM_144770.3         AGTGATAGTGATAGTAGCACAGACAACAACAGAGGCAACGAATGTAGCCAAAAGTTCCGA
                    ************************************************************

HsCD00287690        AAGTCTAAGAAGAAGAAAAGATACTAC
XM_005260996.1      AAGTCTAAGAAGAAGAAAAGATACTAG
NM_144770.3         AAGTCTAAGAAGAAGAAAAGATACTAG
                    **************************