DNASU Plasmid | INTS12 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: INTS12

Gene Name: integrator complex subunit 12

Original Clone ID:

34992

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287856

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1389nts

Open reading frame: 1 to 1389

Reference Sequence Alignment

ATGGCTGCTACTGTGAACTTGGAACTTGATCCCATTTTTTTGAAAGCACTAGGTTTCTTG
CATTCAAAGAGTAAAGATTCTGCTGAAAAGCTAAAAGCACTGCTTGATGAATCTTTGGCT
CGGGGCATTGATTCCAGTTACCGTCCATCTCAAAAGGATGTGGAGCCACCCAAAATTTCA
AGCACAAAAAACATTTCCATTAAGCAAGAGCCCAAAATATCATCCAGTCTTCCTTCTGGT
AATAATAATGGCAAGGTCCTCACAACTGAAAAGGTAAAGAAGGAAGCTGAAAAGAGACCT
GCTGATAAAATGAAATCAGACATCACTGAAGGAGTTGATATTCCAAAGAAACCTAGATTG
GAGAAACCAGAAACACAGTCATCTCCCATTACTGTCCAAAGTAGCAAGGATTTACCTATG
GCTGACCTTTCCAGTTTTGAGGAGACCAGTGCTGATGATTTTGCCATGGAGATGGGATTG
GCCTGCGTTGTTTGTAGGCAAATGATGGTGGCATCTGGCAATCAATTAGTAGAATGTCAG
GAGTGCCATAATCTCTACCACCGAGATTGTCATAAACCCCAGGTGACAGACAAGGAAGCG
AATGACCCTCGCCTGGTGTGGTATTGTGCCCGATGTACCAGACAAATGAAAAGAATGGCT
CAAAAAACTCAGAAACCACCGCAGAAACCAGCCCCTGCAGTTGTTTCTGTAACTCCAGCT
GTCAAAGATCCATTGGTTAAGAAACCAGAAACTAAACTGAAACAAGAGACACTTTTTCTA
GCGTTTAAGAGAACAGAAGTCAAGACATCCACAGTTATTTCAGGAAATTCTTCTAGTGCC
AGCGTTTCCTCGTCAGTAACTAGTGGCTTAACTGGATGGGCAGCTTTTGCAGCCAAAACT
TCCTCTGCTGGTCCTTCAACAGCAAAATTGAGTTCAACAACACAAAACAATACTGGGAAA
CCTGCTACTTCGTCAGCTAACCAGAAACCTGTGGGTTTGACTGGTCTGGCAACATCATCC
AAAGGTGGAATAGGTTCCAAAATAGGTTCCAATAACAGCACTACGCCCACTGTACCTTTA
AAACCACCTCCACCTCTAACCTTGGGTAAAACTGGCCTTAGTCGCTCAGTTAGTTGTGAC
AATGTCAGCAAAGTAGGTCTTCCTAGTCCAAGTAGTTTAGTTCCAGGAAGCAGCAGCCAA
CTAAGTGGGAATGGAAATAGTGGAACATCAGGACCTAGTGGAAGTACTACCAGCAAAACT
ACTTCAGAATCCAGCAGCTCTCCCTCAGCATCCCTTAAAGGCCCAACTTCACAAGAATCA
CAGCTCAATGCTATGAAGCGATTACAGATGGTCAAGAAGAAAGCTGCCCAAAAGAAACTC
AAGAAGTAC

Protein sequence predicted based on nucleotide sequence

MAATVNLELDPIFLKALGFLHSKSKDSAEKLKALLDESLARGIDSSYRPSQKDVEPPKIS
STKNISIKQEPKISSSLPSGNNNGKVLTTEKVKKEAEKRPADKMKSDITEGVDIPKKPRL
EKPETQSSPITVQSSKDLPMADLSSFEETSADDFAMEMGLACVVCRQMMVASGNQLVECQ
ECHNLYHRDCHKPQVTDKEANDPRLVWYCARCTRQMKRMAQKTQKPPQKPAPAVVSVTPA
VKDPLVKKPETKLKQETLFLAFKRTEVKTSTVISGNSSSASVSSSVTSGLTGWAAFAAKT
SSAGPSTAKLSSTTQNNTGKPATSSANQKPVGLTGLATSSKGGIGSKIGSNNSTTPTVPL
KPPPPLTLGKTGLSRSVSCDNVSKVGLPSPSSLVPGSSSQLSGNGNSGTSGPSGSTTSKT
TSESSSSPSASLKGPTSQESQLNAMKRLQMVKKKAAQKKLKKY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1595

Start on reference sequence 207

Mutations:

None

Discrepancy:

a772c,c773t,T258L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

INTS12

Symbol Nomenclature :

INTS12

SYNONYM :

INT12; PHF22

Designation :

PHD finger protein 22|hypothetical nuclear factor SBBI22

Full Nomenclature :

integrator complex subunit 12

GENEID :

57117

Locus Tag :

SBBI22

HGNC :

25067

MIM :

611355

Vega :

OTTHUMG00000131215

Target GenBank:

BC014442.1

Reference Sequence Alignment

  

HsCD00287856        ATGGCTGCTACTGTGAACTTGGAACTTGATCCCATTTTTTTGAAAGCACTAGGTTTCTTG
NM_020395.3         ATGGCTGCTACTGTGAACTTGGAACTTGATCCCATTTTTTTGAAAGCACTAGGTTTCTTG
NM_001142471.1      ATGGCTGCTACTGTGAACTTGGAACTTGATCCCATTTTTTTGAAAGCACTAGGTTTCTTG
                    ************************************************************

HsCD00287856        CATTCAAAGAGTAAAGATTCTGCTGAAAAGCTAAAAGCACTGCTTGATGAATCTTTGGCT
NM_020395.3         CATTCAAAGAGTAAAGATTCTGCTGAAAAGCTAAAAGCACTGCTTGATGAATCTTTGGCT
NM_001142471.1      CATTCAAAGAGTAAAGATTCTGCTGAAAAGCTAAAAGCACTGCTTGATGAATCTTTGGCT
                    ************************************************************

HsCD00287856        CGGGGCATTGATTCCAGTTACCGTCCATCTCAAAAGGATGTGGAGCCACCCAAAATTTCA
NM_020395.3         CGGGGCATTGATTCCAGTTACCGTCCATCTCAAAAGGATGTGGAGCCACCCAAAATTTCA
NM_001142471.1      CGGGGCATTGATTCCAGTTACCGTCCATCTCAAAAGGATGTGGAGCCACCCAAAATTTCA
                    ************************************************************

HsCD00287856        AGCACAAAAAACATTTCCATTAAGCAAGAGCCCAAAATATCATCCAGTCTTCCTTCTGGT
NM_020395.3         AGCACAAAAAACATTTCCATTAAGCAAGAGCCCAAAATATCATCCAGTCTTCCTTCTGGT
NM_001142471.1      AGCACAAAAAACATTTCCATTAAGCAAGAGCCCAAAATATCATCCAGTCTTCCTTCTGGT
                    ************************************************************

HsCD00287856        AATAATAATGGCAAGGTCCTCACAACTGAAAAGGTAAAGAAGGAAGCTGAAAAGAGACCT
NM_020395.3         AATAATAATGGCAAGGTCCTCACAACTGAAAAGGTAAAGAAGGAAGCTGAAAAGAGACCT
NM_001142471.1      AATAATAATGGCAAGGTCCTCACAACTGAAAAGGTAAAGAAGGAAGCTGAAAAGAGACCT
                    ************************************************************

HsCD00287856        GCTGATAAAATGAAATCAGACATCACTGAAGGAGTTGATATTCCAAAGAAACCTAGATTG
NM_020395.3         GCTGATAAAATGAAATCAGACATCACTGAAGGAGTTGATATTCCAAAGAAACCTAGATTG
NM_001142471.1      GCTGATAAAATGAAATCAGACATCACTGAAGGAGTTGATATTCCAAAGAAACCTAGATTG
                    ************************************************************

HsCD00287856        GAGAAACCAGAAACACAGTCATCTCCCATTACTGTCCAAAGTAGCAAGGATTTACCTATG
NM_020395.3         GAGAAACCAGAAACACAGTCATCTCCCATTACTGTCCAAAGTAGCAAGGATTTACCTATG
NM_001142471.1      GAGAAACCAGAAACACAGTCATCTCCCATTACTGTCCAAAGTAGCAAGGATTTACCTATG
                    ************************************************************

HsCD00287856        GCTGACCTTTCCAGTTTTGAGGAGACCAGTGCTGATGATTTTGCCATGGAGATGGGATTG
NM_020395.3         GCTGACCTTTCCAGTTTTGAGGAGACCAGTGCTGATGATTTTGCCATGGAGATGGGATTG
NM_001142471.1      GCTGACCTTTCCAGTTTTGAGGAGACCAGTGCTGATGATTTTGCCATGGAGATGGGATTG
                    ************************************************************

HsCD00287856        GCCTGCGTTGTTTGTAGGCAAATGATGGTGGCATCTGGCAATCAATTAGTAGAATGTCAG
NM_020395.3         GCCTGCGTTGTTTGTAGGCAAATGATGGTGGCATCTGGCAATCAATTAGTAGAATGTCAG
NM_001142471.1      GCCTGCGTTGTTTGTAGGCAAATGATGGTGGCATCTGGCAATCAATTAGTAGAATGTCAG
                    ************************************************************

HsCD00287856        GAGTGCCATAATCTCTACCACCGAGATTGTCATAAACCCCAGGTGACAGACAAGGAAGCG
NM_020395.3         GAGTGCCATAATCTCTACCACCGAGATTGTCATAAACCCCAGGTGACAGACAAGGAAGCG
NM_001142471.1      GAGTGCCATAATCTCTACCACCGAGATTGTCATAAACCCCAGGTGACAGACAAGGAAGCG
                    ************************************************************

HsCD00287856        AATGACCCTCGCCTGGTGTGGTATTGTGCCCGATGTACCAGACAAATGAAAAGAATGGCT
NM_020395.3         AATGACCCTCGCCTGGTGTGGTATTGTGCCCGATGTACCAGACAAATGAAAAGAATGGCT
NM_001142471.1      AATGACCCTCGCCTGGTGTGGTATTGTGCCCGATGTACCAGACAAATGAAAAGAATGGCT
                    ************************************************************

HsCD00287856        CAAAAAACTCAGAAACCACCGCAGAAACCAGCCCCTGCAGTTGTTTCTGTAACTCCAGCT
NM_020395.3         CAAAAAACTCAGAAACCACCGCAGAAACCAGCCCCTGCAGTTGTTTCTGTAACTCCAGCT
NM_001142471.1      CAAAAAACTCAGAAACCACCGCAGAAACCAGCCCCTGCAGTTGTTTCTGTAACTCCAGCT
                    ************************************************************

HsCD00287856        GTCAAAGATCCATTGGTTAAGAAACCAGAAACTAAACTGAAACAAGAGACACTTTTTCTA
NM_020395.3         GTCAAAGATCCATTGGTTAAGAAACCAGAAACTAAACTGAAACAAGAGACAACTTTTCTA
NM_001142471.1      GTCAAAGATCCATTGGTTAAGAAACCAGAAACTAAACTGAAACAAGAGACAACTTTTCTA
                    ***************************************************. *******

HsCD00287856        GCGTTTAAGAGAACAGAAGTCAAGACATCCACAGTTATTTCAGGAAATTCTTCTAGTGCC
NM_020395.3         GCGTTTAAGAGAACAGAAGTCAAGACATCCACAGTTATTTCAGGAAATTCTTCTAGTGCC
NM_001142471.1      GCGTTTAAGAGAACAGAAGTCAAGACATCCACAGTTATTTCAGGAAATTCTTCTAGTGCC
                    ************************************************************

HsCD00287856        AGCGTTTCCTCGTCAGTAACTAGTGGCTTAACTGGATGGGCAGCTTTTGCAGCCAAAACT
NM_020395.3         AGCGTTTCCTCGTCAGTAACTAGTGGCTTAACTGGATGGGCAGCTTTTGCAGCCAAAACT
NM_001142471.1      AGCGTTTCCTCGTCAGTAACTAGTGGCTTAACTGGATGGGCAGCTTTTGCAGCCAAAACT
                    ************************************************************

HsCD00287856        TCCTCTGCTGGTCCTTCAACAGCAAAATTGAGTTCAACAACACAAAACAATACTGGGAAA
NM_020395.3         TCCTCTGCTGGTCCTTCAACAGCAAAATTGAGTTCAACAACACAAAACAATACTGGGAAA
NM_001142471.1      TCCTCTGCTGGTCCTTCAACAGCAAAATTGAGTTCAACAACACAAAACAATACTGGGAAA
                    ************************************************************

HsCD00287856        CCTGCTACTTCGTCAGCTAACCAGAAACCTGTGGGTTTGACTGGTCTGGCAACATCATCC
NM_020395.3         CCTGCTACTTCGTCAGCTAACCAGAAACCTGTGGGTTTGACTGGTCTGGCAACATCATCC
NM_001142471.1      CCTGCTACTTCGTCAGCTAACCAGAAACCTGTGGGTTTGACTGGTCTGGCAACATCATCC
                    ************************************************************

HsCD00287856        AAAGGTGGAATAGGTTCCAAAATAGGTTCCAATAACAGCACTACGCCCACTGTACCTTTA
NM_020395.3         AAAGGTGGAATAGGTTCCAAAATAGGTTCCAATAACAGCACTACGCCCACTGTACCTTTA
NM_001142471.1      AAAGGTGGAATAGGTTCCAAAATAGGTTCCAATAACAGCACTACGCCCACTGTACCTTTA
                    ************************************************************

HsCD00287856        AAACCACCTCCACCTCTAACCTTGGGTAAAACTGGCCTTAGTCGCTCAGTTAGTTGTGAC
NM_020395.3         AAACCACCTCCACCTCTAACCTTGGGTAAAACTGGCCTTAGTCGCTCAGTTAGTTGTGAC
NM_001142471.1      AAACCACCTCCACCTCTAACCTTGGGTAAAACTGGCCTTAGTCGCTCAGTTAGTTGTGAC
                    ************************************************************

HsCD00287856        AATGTCAGCAAAGTAGGTCTTCCTAGTCCAAGTAGTTTAGTTCCAGGAAGCAGCAGCCAA
NM_020395.3         AATGTCAGCAAAGTAGGTCTTCCTAGTCCAAGTAGTTTAGTTCCAGGAAGCAGCAGCCAA
NM_001142471.1      AATGTCAGCAAAGTAGGTCTTCCTAGTCCAAGTAGTTTAGTTCCAGGAAGCAGCAGCCAA
                    ************************************************************

HsCD00287856        CTAAGTGGGAATGGAAATAGTGGAACATCAGGACCTAGTGGAAGTACTACCAGCAAAACT
NM_020395.3         CTAAGTGGGAATGGAAATAGTGGAACATCAGGACCTAGTGGAAGTACTACCAGCAAAACT
NM_001142471.1      CTAAGTGGGAATGGAAATAGTGGAACATCAGGACCTAGTGGAAGTACTACCAGCAAAACT
                    ************************************************************

HsCD00287856        ACTTCAGAATCCAGCAGCTCTCCCTCAGCATCCCTTAAAGGCCCAACTTCACAAGAATCA
NM_020395.3         ACTTCAGAATCCAGCAGCTCTCCCTCAGCATCCCTTAAAGGCCCAACTTCACAAGAATCA
NM_001142471.1      ACTTCAGAATCCAGCAGCTCTCCCTCAGCATCCCTTAAAGGCCCAACTTCACAAGAATCA
                    ************************************************************

HsCD00287856        CAGCTCAATGCTATGAAGCGATTACAGATGGTCAAGAAGAAAGCTGCCCAAAAGAAACTC
NM_020395.3         CAGCTCAATGCTATGAAGCGATTACAGATGGTCAAGAAGAAAGCTGCCCAAAAGAAACTC
NM_001142471.1      CAGCTCAATGCTATGAAGCGATTACAGATGGTCAAGAAGAAAGCTGCCCAAAAGAAACTC
                    ************************************************************

HsCD00287856        AAGAAGTAC
NM_020395.3         AAGAAGTAA
NM_001142471.1      AAGAAGTAA
                    ********.