DNASU Plasmid | ZFAND2B (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ZFAND2B

Gene Name: zinc finger, AN1-type domain 2B

Original Clone ID:

27864

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287914

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 774nts

Open reading frame: 1 to 774

Reference Sequence Alignment

ATGGAGTTTCCGGACCTCGGCGCTCACTGTTCGGAGCCGAGCTGTCAGCGCTTGGATTTT
CTGCCGCTTAAGTGTGATGCCTGCTCAGGCATCTTCTGCGCAGACCATGTGGCCTACGCC
CAGCATCACTGTGGATCTGCTTACCAAAAGGATATCCAGGTACCTGTGTGCCCTCTCTGT
AATGTGCCTGTGCCTGTGGCCAGAGGGGAGCCCCCTGACCGTGCTGTGGGAGAGCACATT
GACAGAGACTGTCGCTCTGATCCAGCACAGCAAAAACGTAAGATCTTCACCAATAAGTGT
GAACGCGCTGGCTGCCGGCAGCGAGAAATGATGAAACTGACCTGTGAACGCTGTAGCCGA
AACTTCTGCATCAAGCACCGGCATCCACTGGACCATGATTGCTCTGGGGAGGGGCACCCA
ACCAGCCGGGCAGGACTTGCTGCCATCTCCAGAGCACAAGCTGTGGCTTCTACAAGCACT
GTCCCCAGCCCAAGTCAAACCATGCCTTCCTGTACCTCTCCCAGCAGAGCCACAACCCGA
TCTCCGTCCTGGACAGCCCCTCCAGTGATTGCTTTGCAGAATGGCCTGAGTGAGGATGAA
GCTCTGCAGCGGGCCCTGGAAATGTCCCTGGCAGAAACCAAACCCCAGGTTCCAAGTTGT
CAGGAGGAAGAAGACCTAGCTTTAGCACAAGCACTGTCAGCCAGTGAGGCAGAATACCAG
CGGCAGCAGGCCCAGAGCCGCAGCTCGAAGCCGTCCAACTGCAGCCAGTGCTAC

Protein sequence predicted based on nucleotide sequence

MEFPDLGAHCSEPSCQRLDFLPLKCDACSGIFCADHVAYAQHHCGSAYQKDIQVPVCPLC
NVPVPVARGEPPDRAVGEHIDRDCRSDPAQQKRKIFTNKCERAGCRQREMMKLTCERCSR
NFCIKHRHPLDHDCSGEGHPTSRAGLAAISRAQAVASTSTVPSPSQTMPSCTSPSRATTR
SPSWTAPPVIALQNGLSEDEALQRALEMSLAETKPQVPSCQEEEDLALAQALSASEAEYQ
RQQAQSRSSKPSNCSQCY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 173

End on reference sequence 946

Mutations:

None

Discrepancy:

t767a,L256Q

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

ZFAND2B

Symbol Nomenclature :

ZFAND2B

SYNONYM :

AIRAPL

Designation :

AIRAP-like protein|AN1-type zinc finger protein 2B|arsenite-inducible RNA-associated protein-like protein

Full Nomenclature :

zinc finger, AN1-type domain 2B

GENEID :

130617

HGNC :

25206

MIM :

613474

Vega :

OTTHUMG00000133135

Target GenBank:

BC018415.1

Reference Sequence Alignment

  

HsCD00287914        ATGGAGTTTCCGGACCTCGGCGCTCACTGTTCGGAGCCGAGCTGTCAGCGCTTGGATTTT
NM_138802.2         ATGGAGTTTCCGGACCTCGGCGCTCACTGTTCGGAGCCGAGCTGTCAGCGCTTGGATTTT
NM_001270998.1      ATGGAGTTTCCGGACCTCGGCGCTCACTGTTCGGAGCCGAGCTGTCAGCGCTTGGATTTT
                    ************************************************************

HsCD00287914        CTGCCGCTTAAGTGTGATGCCTGCTCAGGCATCTTCTGCGCAGACCATGTGGCCTACGCC
NM_138802.2         CTGCCGCTTAAGTGTGATGCCTGCTCAGGCATCTTCTGCGCAGACCATGTGGCCTACGCC
NM_001270998.1      CTGCCGCTTAAGTGTGATGCCTGCTCAGGCATCTTCTGCGCAGACCATGTGGCCTACGCC
                    ************************************************************

HsCD00287914        CAGCATCACTGTGGATCTGCTTACCAAAAGGATATCCAGGTACCTGTGTGCCCTCTCTGT
NM_138802.2         CAGCATCACTGTGGATCTGCTTACCAAAAGGATATCCAGGTACCTGTGTGCCCTCTCTGT
NM_001270998.1      CAGCATCACTGTGGATCTGCTTACCAAAAGGATATCCAGGTACCTGTGTGCCCTCTCTGT
                    ************************************************************

HsCD00287914        AATGTGCCTGTGCCTGTGGCCAGAGGGGAGCCCCCTGACCGTGCTGTGGGAGAGCACATT
NM_138802.2         AATGTGCCTGTGCCTGTGGCCAGAGGGGAGCCCCCTGACCGTGCTGTGGGAGAGCACATT
NM_001270998.1      AATGTGCCTGTGCCTGTGGCCAGAGGGGAGCCCCCTGACCGTGCTGTGGGAGAGCACATT
                    ************************************************************

HsCD00287914        GACAGAGACTGTCGCTCTGATCCAGCACAGCAAAAACGTAAGATCTTCACCAATAAGTGT
NM_138802.2         GACAGAGACTGTCGCTCTGATCCAGCACAGCAAAAACGTAAGATCTTCACCAATAAGTGT
NM_001270998.1      GACAGAGACTGTCGCTCTGATCCAGCACAGCAAAAACGTAAGATCTTCACCAATAAGTGT
                    ************************************************************

HsCD00287914        GAACGCGCTGGCTGCCGGCAGCGAGAAATGATGAAACTGACCTGTGAACGCTGTAGCCGA
NM_138802.2         GAACGCGCTGGCTGCCGGCAGCGAGAAATGATGAAACTGACCTGTGAACGCTGTAGCCGA
NM_001270998.1      GAACGCGCTGGCTGCCGGCAGCGAGAAATGATGAAACTGACCTGTGAACGCTGTAGCCGA
                    ************************************************************

HsCD00287914        AACTTCTGCATCAAGCACCGGCATCCACTGGACCATGATTGCTCTGGGGAGGGGCACCCA
NM_138802.2         AACTTCTGCATCAAGCACCGGCATCCACTGGACCATGATTGCTCTGGGGAGGGGCACCCA
NM_001270998.1      AACTTCTGCATCAAGCACCGGCATCCACTGGACCATGATTGCTCTGGGGAGGGGCACCCA
                    ************************************************************

HsCD00287914        ACCAGCCGGGCAGGACTTGCTGCCATCTCCAGAGCACAAGCTGTGGCTTCTACAAGCACT
NM_138802.2         ACCAGCCGGGCAGGACTTGCTGCCATCTCCAGAGCACAAGCTGTGGCTTCTACAAGCACT
NM_001270998.1      ACCAGCCGGGCAGGACTTGCTGCCATCTCCAGAGCACAAGCTGTGGCTTCTACAAGCACT
                    ************************************************************

HsCD00287914        GTCCCCAGCCCAAGTCAAACCATGCCTTCCTGTACCTCTCCCAGCAGAGCCACAACCCGA
NM_138802.2         GTCCCCAGCCCAAGTCAAACCATGCCTTCCTGTACCTCTCCCAGCAGAGCCACAACCCGA
NM_001270998.1      GTCCCCAGCCCAAGTCAAACCATGCCTTCCTGTACCTCTCCCAGCAGAGCCACAACCCGA
                    ************************************************************

HsCD00287914        TCTCCGTCCTGGACAGCCCCTCCAGTGATTGCTTTGCAGAATGGCCTGAGTGAGGATGAA
NM_138802.2         TCTCCGTCCTGGACAGCCCCTCCAGTGATTGCTTTGCAGAATGGCCTGAGTGAGGATGAA
NM_001270998.1      TCTCCGTCCTGGACAGCCCCTCCAGTGATTGCTTTGCAGAATGGCCTGAGTGAGGATGAA
                    ************************************************************

HsCD00287914        GCTCTGCAGCGGGCCCTGGAAATGTCCCTGGCAGAAACCAAACCCCAGGTTCCAAGTTGT
NM_138802.2         GCTCTGCAGCGGGCCCTGGAAATGTCCCTGGCAGAAACCAAACCCCAGGTTCCAAGTTGT
NM_001270998.1      GCTCTGCAGCGGGCCCTGGAAATGTCCCTGGCAGAAACCAAACCCCAGGTTCCAAGTTGT
                    ************************************************************

HsCD00287914        CAGGAGGAAGAAGACCTAGCTTTAGCACAAGCACTGTCAGCCAGTGAGGCAGAATACCAG
NM_138802.2         CAGGAGGAAGAAGACCTAGCTTTAGCACAAGCACTGTCAGCCAGTGAGGCAGAATACCAG
NM_001270998.1      CAGGAGGAAGAAGACCTAGCTTTAGCACAAGCACTGTCAGCCAGTGAGGCAGAATACCAG
                    ************************************************************

HsCD00287914        CGGCAGCAGGCCCAGAGCCGCAGCTCGAAGCCGTCCAACTGCAGCCAGTGCTAC
NM_138802.2         CGGCAGCAGGCCCAGAGCCGCAGCTCGAAGCCGTCCAACTGCAGCCTGTGCTAG
NM_001270998.1      CGGCAGCAGGCCCAGAGCCGCAGCTCGAAGCCGTCCAACTGCAGCCTGTGCTAG
                    **********************************************:******