DNASU Plasmid | C18orf1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: LDLRAD4

Gene Name: low density lipoprotein receptor class A domain containing 4

Original Clone ID:

43988

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00287996

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 867nts

Open reading frame: 1 to 867

Reference Sequence Alignment

ATGCCGGAAGCTGGTTTTCAGGCCACAAATGCTTTCACAGAGTGCAAATTCACCTGCACC
AGTGGTAAATGCTTGTATCTTGGTTCGCTGGTCTGTAACCAACAGAACGACTGTGGGGAC
AACAGTGACGAAGAGAACTGTCTCCTGGTGACCGAGCACCCGCCTCCGGGCATCTTCAAC
TCGGAGCTGGAGTTCGCCCAAATCATCATCATCGTCGTGGTGGTCACGGTGATGGTGGTG
GTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGCGGTCCTTCATCAACCGCCCG
AACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAGATCATGCATGCCCCGCGGTCCAGG
GACAGGTTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTCCAGCCCACC
TACCCCTATGTGCAGCACGAGATTGATCTTCCTCCCACCATCTCCCTGTCCGACGGTGAA
GAGCCACCTCCTTACCAGGGGCCCTGCACCCTGCAGCTCCGGGACCCTGAACAGCAGATG
GAACTCAACCGAGAGTCCGTGAGGGCCCCACCCAACCGAACCATATTTGACAGTGATTTA
ATAGACATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCAACTCGGGCATCAGT
GCAAGCACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATACAGCGAGGTG
ATGGGCCACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCACACAGGGGC
AGCAGACTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATCAAAGGCAAA
GATAGGAAGCCTGGGAACCTGGTCTGC

Protein sequence predicted based on nucleotide sequence

MPEAGFQATNAFTECKFTCTSGKCLYLGSLVCNQQNDCGDNSDEENCLLVTEHPPPGIFN
SELEFAQIIIIVVVVTVMVVVIVCLLNHYKVSTRSFINRPNQSRRREDGLPQIMHAPRSR
DRFTAPSFIQRDRFSRFQPTYPYVQHEIDLPPTISLSDGEEPPPYQGPCTLQLRDPEQQM
ELNRESVRAPPNRTIFDSDLIDIAMYSGGPCPPSSNSGISASTCSSNGRMEGPPPTYSEV
MGHHPGASFLHHQRSNAHRGSRLQFQQNNAESTIVPIKGKDRKPGNLVC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1526

Start on reference sequence 660

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

LDLRAD4

Symbol Nomenclature :

LDLRAD4

SYNONYM :

C18orf1

Designation :

clone 22|low-density lipoprotein receptor class A domain-containing protein 4

Full Nomenclature :

low density lipoprotein receptor class A domain containing 4

GENEID :

753

HGNC :

1224

MIM :

606571

Vega :

OTTHUMG00000181925

Target GenBank:

BC030199.1

Reference Sequence Alignment

  

HsCD00287996        ATGCCGGAAGCTGGTTTTCAGGCCACAAATGCTTTCACAGAGTGCAAATTCACCTGCACC
NM_181481.4         ATGCCGGAAGCTGGTTTTCAGGCCACAAATGCTTTCACAGAGTGCAAATTCACCTGCACC
NM_181482.4         ATGCCGGAAGCTGGTTTTCAGGCCACAAATGCTTTCACAGAGTGCAAATTCACCTGCACC
NM_001003674.3      ------------------------------------------------------------
XM_005258140.1      ATGCCGGAAGCTGGTTTTCAGGCCACAAATGCTTTCACAGAGTGCAAATTCACCTGCACC
                                                                                

HsCD00287996        AGTGGTAAATGCTTGTATCTTGGTTCGCTGGTCTGTAACCAACAGAACGACTGTGGGGAC
NM_181481.4         AGTGGTAAATGCTTGTATCTTGGTTCGCTGGTCTGTAACCAACAGAACGACTGTGGGGAC
NM_181482.4         AGTGGTAAATGCTTGTATCTTGGTTCGCTGGTCTGTAACCAACAGAACGACTGTGGGGAC
NM_001003674.3      ---------------------------------------------------ATGTCCAGT
XM_005258140.1      AGTGGTAAATGCTTGTATCTTGGTTCGCTGGTCTGTAACCAACAGAACGACTGTGGGGAC
                                                                       :     .. 

HsCD00287996        AACAGTGACGAAGAGAACTGTCTCCTGGTGACCGAGCACCCGCCTCCGGGCATCTTCAAC
NM_181481.4         AACAGTGACGAAGAGAACTGTCTCCTGGTGACCGAGCACCCGCCTCCGGGCATCTTCAAC
NM_181482.4         AACAGTGACGAAGAGAACTGTCTCCTGGTGACCGAGCACCCGCCTCCGGGCATCTTCAAC
NM_001003674.3      GACCACCTGAACAACAGCACACTGAAGGAGGCTCAGTTCAAAGACCTGTTCTTAAAAAAA
XM_005258140.1      AACAGTGACGAAGAGAACTGTCTCCTGGTGACCGAGCACCCGCCTCCGGGCATCTTCAAC
                    .**..  : .*..* *.*: :** .:**:*.*  ** :*... . * *  *:*.::.**.

HsCD00287996        TCGGAGCTGGAGTTCGCCCAAATCATCATCATCGTCGTGGTGGTCACGGTGATGGTGGTG
NM_181481.4         TCGGAGCTGGAGTTCGCCCAAATCATCATCATCGTCGTGGTGGTCACGGTGATGGTGGTG
NM_181482.4         TCGGAGCTGGAGTTCGCCCAAATCATCATCATCGTCGTGGTGGTCACGGTGATGGTGGTG
NM_001003674.3      GCGGAGCTGGAGTTCGCCCAAATCATCATCATCGTCGTGGTGGTCACGGTGATGGTGGTG
XM_005258140.1      TCGGAGCTGGAGTTCGCCCAAATCATCATCATCGTCGTGGTGGTCACGGTGATGGTGGTG
                     ***********************************************************

HsCD00287996        GTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGCGGTCCTTCATCAACCGCCCG
NM_181481.4         GTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGCGGTCCTTCATCAACCGCCCG
NM_181482.4         GTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGCGGTCCTTCATCAACCGCCCG
NM_001003674.3      GTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGCGGTCCTTCATCAACCGCCCG
XM_005258140.1      GTCATCGTCTGCCTGCTGAACCACTACAAAGTCTCCACGCGGTCCTTCATCAACCGCCCG
                    ************************************************************

HsCD00287996        AACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAG------------------------
NM_181481.4         AACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAGGAAGGGTGCCTGTGGCCTTCAGAC
NM_181482.4         AACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAG------------------------
NM_001003674.3      AACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAGGAAGGGTGCCTGTGGCCTTCAGAC
XM_005258140.1      AACCAGAGCCGGAGGCGGGAGGACGGGCTGCCGCAGGAAGGGTGCCTGTGGCCTTCAGAC
                    ************************************                        

HsCD00287996        ------------------------------ATCATGCATGCCCCGCGGTCCAGGGACAGG
NM_181481.4         AGCGCCGCACCGCGGCTGGGCGCCTCGGAGATCATGCATGCCCCGCGGTCCAGGGACAGG
NM_181482.4         ------------------------------ATCATGCATGCCCCGCGGTCCAGGGACAGG
NM_001003674.3      AGCGCCGCACCGCGGCTGGGCGCCTCGGAGATCATGCATGCCCCGCGGTCCAGGGACAGG
XM_005258140.1      AGCGCCGCACCGCGGCTGGGCGCCTCGGAGATCATGCATGCCCCGCGGTCCAGGGACAGG
                                                  ******************************

HsCD00287996        TTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTCCAGCCCACCTACCCC
NM_181481.4         TTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTCCAGCCCACCTACCCC
NM_181482.4         TTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTCCAGCCCACCTACCCC
NM_001003674.3      TTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTCCAGCCCACCTACCCC
XM_005258140.1      TTCACAGCGCCGTCCTTCATCCAGAGGGATCGCTTCAGCCGCTTCCAGCCCACCTACCCC
                    ************************************************************

HsCD00287996        TATGTGCAGCACGAGATTGATCTTCCTCCCACCATCTCCCTGTCCGACGGTGAAGAGCCA
NM_181481.4         TATGTGCAGCACGAGATTGATCTTCCTCCCACCATCTCCCTGTCCGACGGTGAAGAGCCA
NM_181482.4         TATGTGCAGCACGAGATTGATCTTCCTCCCACCATCTCCCTGTCCGACGGTGAAGAGCCA
NM_001003674.3      TATGTGCAGCACGAGATTGATCTTCCTCCCACCATCTCCCTGTCCGACGGTGAAGAGCCA
XM_005258140.1      TATGTGCAGCACGAGATTGATCTTCCTCCCACCATCTCCCTGTCCGACGGTGAAGAGCCA
                    ************************************************************

HsCD00287996        CCTCCTTACCAGGGGCCCTGCACCCTGCAGCTCCGGGACCCTGAACAGCAGATGGAACTC
NM_181481.4         CCTCCTTACCAGGGGCCCTGCACCCTGCAGCTCCGGGACCCTGAACAGCAGATGGAACTC
NM_181482.4         CCTCCTTACCAGGGGCCCTGCACCCTGCAGCTCCGGGACCCTGAACAGCAGATGGAACTC
NM_001003674.3      CCTCCTTACCAGGGGCCCTGCACCCTGCAGCTCCGGGACCCTGAACAGCAGATGGAACTC
XM_005258140.1      CCTCCTTACCAGGGGCCCTGCACCCTGCAGCTCCGGGACCCTGAACAGCAGATGGAACTC
                    ************************************************************

HsCD00287996        AACCGAGAGTCCGTGAGGGCCCCACCCAACCGAACCATATTTGACAGTGATTTAATAGAC
NM_181481.4         AACCGAGAGTCCGTGAGGGCCCCACCCAACCGAACCATATTTGACAGTGATTTAATAGAC
NM_181482.4         AACCGAGAGTCCGTGAGGGCCCCACCCAACCGAACCATATTTGACAGTGATTTAATAGAC
NM_001003674.3      AACCGAGAGTCCGTGAGGGCCCCACCCAACCGAACCATATTTGACAGTGATTTAATAGAC
XM_005258140.1      AACCGAGAGTCCGTGAGGGCCCCACCCAACCGAACCATATTTGACAGTGATTTAATAGAC
                    ************************************************************

HsCD00287996        ATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCAACTCGGGCATCAGTGCAAGC
NM_181481.4         ATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCAACTCGGGCATCAGTGCAAGC
NM_181482.4         ATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCAACTCGGGCATCAGTGCAAGC
NM_001003674.3      ATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCAACTCGGGCATCAGTGCAAGC
XM_005258140.1      ATTGCTATGTATAGCGGGGGTCCATGCCCACCCAGCAGCAACTCGGGCATCAGTGCAAGC
                    ************************************************************

HsCD00287996        ACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATACAGCGAGGTGATGGGC
NM_181481.4         ACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATACAGCGAGGTGATGGGC
NM_181482.4         ACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATACAGCGAGGTGATGGGC
NM_001003674.3      ACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATACAGCGAGGTGATGGGC
XM_005258140.1      ACCTGCAGCAGTAACGGGAGGATGGAGGGGCCACCCCCCACATACAGCGAGGTGATGGGC
                    ************************************************************

HsCD00287996        CACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCACACAGGGGCAGCAGA
NM_181481.4         CACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCACACAGGGGCAGCAGA
NM_181482.4         CACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCACACAGGGGCAGCAGA
NM_001003674.3      CACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCACACAGGGGCAGCAGA
XM_005258140.1      CACCACCCAGGCGCCTCTTTCCTCCATCACCAGCGCAGCAACGCACACAGGGGCAGCAGA
                    ************************************************************

HsCD00287996        CTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATCAAAGGCAAAGATAGG
NM_181481.4         CTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATCAAAGGCAAAGATAGG
NM_181482.4         CTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATCAAAGGCAAAGATAGG
NM_001003674.3      CTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATCAAAGGCAAAGATAGG
XM_005258140.1      CTGCAGTTTCAGCAGAACAATGCAGAGAGCACAATAGTACCCATCAAAGGCAAAGATAGG
                    ************************************************************

HsCD00287996        AAGCCTGGGAACCTGGTCTGC
NM_181481.4         AAGCCTGGGAACCTGGTCTGA
NM_181482.4         AAGCCTGGGAACCTGGTCTGA
NM_001003674.3      AAGCCTGGGAACCTGGTCTGA
XM_005258140.1      AAGCCTGGGAACCTGGTCTGA
                    ********************.