DNASU Plasmid | BCL2L11 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: BCL2L11

Gene Name: BCL2-like 11 (apoptosis facilitator)

Original Clone ID:

44419

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288010

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 597nts

Open reading frame: 1 to 597

Reference Sequence Alignment

ATGGCAAAGCAACCTTCTGATGTAAGTTCTGAGTGTGACCGAGAAGGTAGACAATTGCAG
CCTGCGGAGAGGCCTCCCCAGCTCAGACCTGGGGCCCCTACCTCCCTACAGACAGAGCCA
CAAGGTAATCCTGAAGGCAATCACGGAGGTGAAGGGGACAGCTGCCCCCACGGCAGCCCT
CAGGGCCCGCTGGCCCCACCTGCCAGCCCTGGCCCTTTTGCTACCAGATCCCCGCTTTTC
ATCTTTATGAGAAGATCCTCCCTGCTGTCTCGATCCTCCAGTGGGTATTTCTCTTTTGAC
ACAGACAGGAGCCCAGCACCCATGAGTTGTGACAAATCAACACAAACCCCAAGTCCTCCT
TGCCAGGCCTTCAACCACTATCTCAGTGCAATGGCTTCCATGAGGCAGGCTGAACCTGCA
GATATGCGCCCAGAGATATGGATCGCCCAAGAGTTGCGGCGTATTGGAGACGAGTTTAAC
GCTTACTATGCAAGGAGGGTATTTTTGAATAATTACCAAGCAGCCGAAGACCACCCACGA
ATGGTTATCTTACGACTGTTACGTTACATTGTCCGCCTGGTGTGGAGAATGCATTGC

Protein sequence predicted based on nucleotide sequence

MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSP
QGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPP
CQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPR
MVILRLLRYIVRLVWRMHC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 271

End on reference sequence 867

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

BCL2L11

Symbol Nomenclature :

BCL2L11

SYNONYM :

BAM; BIM; BOD

Designation :

bcl-2 interacting mediator of cell death|bcl-2 interacting protein Bim|bcl-2-like protein 11|bcl-2-related ovarian death agonist

Full Nomenclature :

BCL2-like 11 (apoptosis facilitator)

GENEID :

10018

HGNC :

994

MIM :

603827

Vega :

OTTHUMG00000131256

Target GenBank:

BC033694.1

Reference Sequence Alignment

  

HsCD00288010        ATGGCAAAGCAACCTTCTGATGTAAGTTCTGAGTGTGACCGAGAAGGTAGACAATTGCAG
NM_138621.4         ATGGCAAAGCAACCTTCTGATGTAAGTTCTGAGTGTGACCGAGAAGGTAGACAATTGCAG
XM_005263551.1      ---ATGTTCCCGGCGGCTGCGGCCGGGGCAGCGCGGGCCAGAGGCGCGGCGTGCGGAGCC
XM_005263559.1      ---ATGTTCCCGGCGGCTGCGGCCGGGGCAGCGCGGGCCAGAGGCGCGGCGTGCGGAGCC
                       . .:: *.. *  ***. * ..*  *:*.* * *.*.***..*  . . ..  . . 

HsCD00288010        CCTGCGGAGAGGCCTCCCCAGCTCAGACCTGGGGCCCCTACCTCCCTACAGACAGAGCCA
NM_138621.4         CCTGCGGAGAGGCCTCCCCAGCTCAGACCTGGGGCCCCTACCTCCCTACAGACAGAGCCA
XM_005263551.1      CTCGGCTGCCCGGCGGAGCGCGGCGGCGGGCTGGCGGGAAGGCGCGGGCTTTGCGCTGCG
XM_005263559.1      CTCGGCTGCCCGGCGGAGCGCGGCGGCGGGCTGGCGGGAAGGCGCGGGCTTTGCGCTGCG
                    *  *   . . * *  . *.   *.*.     ***   :*    *  .*: : .*.  *.

HsCD00288010        CAAGGTAATCCTGAAGGCAATCACGGAGGTGAAGGGGACAGCTGCCCCCACGGCAGCCCT
NM_138621.4         CAAGGTAATCCTGAAGGCAATCACGGAGGTGAAGGGGACAGCTGCCCCCACGGCAGCCCT
XM_005263551.1      CCGGGGACTCTGAACCCGAGTCCCGGGCTTTGTCTCCTGCGCTGCTTTCGTGGTGACGGT
XM_005263559.1      CCGGGGACTCTGAACCCGAGTCCCGGGCTTTGTCTCCTGCGCTGCTTTCGTGGTGACGGT
                    *..** *.**  .*.   *.**.***.  * .:    : .*****   *. ** ..*  *

HsCD00288010        CAGGGCCCGCTGGCCCCACCTG-----CCAGCCCTGGCCCTTTTGCTACCAGATCCCCGC
NM_138621.4         CAGGGCCCGCTGGCCCCACCTG-----CCAGCCCTGGCCCTTTTGCTACCAGATCCCCGC
XM_005263551.1      CAGGGGGCGCCCGGTCGGCGAAGGGCGCGGGCCGGACGCCGCGGGGCTTGGTCCCTGGCC
XM_005263559.1      CAGGGGGCGCCCGGTCGGCGAAGGGCGCGGGCCGGACGCCGCGGGGCTTGGTCCCTGGCC
                    *****  ***  *  * .* :.     * .***  .  **    *  :  . . *    *

HsCD00288010        TTTTCATCTTTATGAGAAGATCCTCCCTGCTGTCTCGATCCTCCAGTGGGTATTTCTCTT
NM_138621.4         TTTTCATCTTTATGAGAAGATCCTCCCTGCTGTCTCGATCCTCCAGTGGGTATTTCTCTT
XM_005263551.1      CGGACGCTGCGCTCTGAAGGGAAGGCGCGGACAAAAAAAGACCAAATGGCAAAGCAACCT
XM_005263559.1      CGGACGCTGCGCTCTGAAGGGAAGGCGCGGACAAAAAAAGACCAAATGGCAAAGCAACCT
                       :*.     .* :****. ..  *  * : :.:..*: . *.*.*** :*:  .:* *

HsCD00288010        TTGACACAGACAGGAGCCCAGCACCCATGAGTTGTGACAAATCAACACAAACCCCAAGTC
NM_138621.4         TTGACACAGACAGGAGCCCAGCACCCATGAGTTGTGACAAATCAACACAAACCCCAAGTC
XM_005263551.1      TCTGATGTAAGTTCTGAGTGTGACCGAGAAGGTAGACAATTGCAGCCTGCGGAGAGGCCT
XM_005263559.1      TCTGATGTAAGTTCTGAGTGTGACCGAGAAGGTAGACAATTGCAGCCTGCGGAGAGGCCT
                    *  ..: :.* :  :*.  .  *** * .** *. ...*:: **.*. ... . ...   

HsCD00288010        CTCCTTGCCAGGCCTTCAACCACTATCTCAGTGCAATGG---------------------
NM_138621.4         CTCCTTGCCAGGCCTTCAACCACTATCTCAGTGCAATGG---------------------
XM_005263551.1      CCCCAGCTCAGACCTGGGGCCCCTACCTCCCTACAGACAGAGCCACAAGGTAATCCTGAA
XM_005263559.1      CCCCAGCTCAGACCTGGGGCCCCTACCTCCCTACAGACAGAGCCACAAG-----------
                    * **:   ***.***  ..**.*** ***. *.**.: .                     

HsCD00288010        ------------------------------------------------------------
NM_138621.4         ------------------------------------------------------------
XM_005263551.1      GGCAATCACGGAGGTGAAGGGGACAGCTGCCCCCACGGCAGCCCTCAGGGCCCGCTGGCC
XM_005263559.1      ------------------------------------------------------------
                                                                                

HsCD00288010        ------------------------------------------------------------
NM_138621.4         ------------------------------------------------------------
XM_005263551.1      CCACCTGCCAGCCCTGGCCCTTTTGCTACCAGATCCCCGCTTTTCATCTTTATGAGAAGA
XM_005263559.1      ------------------------------------------------------------
                                                                                

HsCD00288010        ------------------------------------------------------------
NM_138621.4         ------------------------------------------------------------
XM_005263551.1      TCCTCCCTGCTGTCTCGATCCTCCAGTGGGTATTTCTCTTTTGACACAGACAGGAGCCCA
XM_005263559.1      ------------------------------------------------------------
                                                                                

HsCD00288010        ------------------------------------------------------------
NM_138621.4         ------------------------------------------------------------
XM_005263551.1      GCACCCATGAGTTGTGACAAATCAACACAAACCCCAAGTCCTCCTTGCCAGGCCTTCAAC
XM_005263559.1      ------------------------------------------------------------
                                                                                

HsCD00288010        -------------------CTTCCATGAGGCAGGCTGAACCTGCAGATATGCGCCCAGAG
NM_138621.4         -------------------CTTCCATGAGGCAGGCTGAACCTGCAGATATGCGCCCAGAG
XM_005263551.1      CACTATCTCAGTGCAATGGCTTCCATGAGGCAGGCTGAACCTGCAGATATGCGCCCAGAG
XM_005263559.1      -------------------CTTCCATGAGGCAGGCTGAACCTGCAGATATGCGCCCAGAG
                                       *****************************************

HsCD00288010        ATATGGATCGCCCAAGAGTTGCGGCGTATTGGAGACGAGTTTAACGCTTACTATGCAAGG
NM_138621.4         ATATGGATCGCCCAAGAGTTGCGGCGTATTGGAGACGAGTTTAACGCTTACTATGCAAGG
XM_005263551.1      ATATGGATCGCCCAAGAGTTGCGGCGTATTGGAGACGAGTTTAACGCTTACTATGCAAGG
XM_005263559.1      ATATGGATCGCCCAAGAGTTGCGGCGTATTGGAGACGAGTTTAACGCTTACTATGCAAGG
                    ************************************************************

HsCD00288010        AGGGTATTTTTGAATAATTACCAAGCAGCCGAAGACCACCCACGAATGGTTATCTTACGA
NM_138621.4         AGGGTATTTTTGAATAATTACCAAGCAGCCGAAGACCACCCACGAATGGTTATCTTACGA
XM_005263551.1      AGGGTATTTTTGAATAATTACCAAGCAGCCGAAGACCACCCACGAATGGTTATCTTACGA
XM_005263559.1      AGGGTATTTTTGAATAATTACCAAGCAGCCGAAGACCACCCACGAATGGTTATCTTACGA
                    ************************************************************

HsCD00288010        CTGTTACGTTACATTGTCCGCCTGGTGTGGAGAATGCATTGC
NM_138621.4         CTGTTACGTTACATTGTCCGCCTGGTGTGGAGAATGCATTGA
XM_005263551.1      CTGTTACGTTACATTGTCCGCCTGGTGTGGAGAATGCATTGA
XM_005263559.1      CTGTTACGTTACATTGTCCGCCTGGTGTGGAGAATGCATTGA
                    *****************************************.

Pathways

NCI :

AP-1 transcription factor network

NCI :

FoxO family signaling

NCI :

p73 transcription factor network

NCI :

p75(NTR)-mediated signaling

Panther :

Apoptosis signaling pathway

Reactome :

Activation of BH3-only proteins

Reactome :

Activation of BIM and translocation to mitochondria

Reactome :

Apoptosis

Reactome :

BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members

Reactome :

Cell death signalling via NRAGE, NRIF and NADE

Reactome :

Intrinsic Pathway for Apoptosis

Reactome :

NRAGE signals death through JNK

Reactome :

p75 NTR receptor-mediated signalling

Reactome :

Signal Transduction

Reactome :

Signalling by NGF

WikiPathway :

Apoptosis

WikiPathway :

Apoptosis Modulation and Signaling

WikiPathway :

DNA damage response (only ATM dependent)

WikiPathway :

ErbB signaling pathway

Protein

UniProt :

O43521

HPRD :

04828

Clone

Cloning Information :

471247

HIP Master Clone ID :

394165

Original Clone ID :

44419

Organism

Species Specific ID:

10018

Genome

Chromosome :

Map Location :

2q13

Ensembl :

ENSG00000153094

Reagents

Labome :

Bim-antibody