DNASU Plasmid | RBM45 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: RBM45

Gene Name: RNA binding motif protein 45

Original Clone ID:

45132

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288050

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1425nts

Open reading frame: 1 to 1425

Reference Sequence Alignment

ATGGACGAAGCTGGCAGCTCTGCGAGCGGCGGGGGCTTCCGCCCGGGCGTGGACAGCCTG
GACGAACCGCCCAACAGCCGCATCTTCCTTGTGATCAGCAAGTACACACCTGAGTCGGTG
CTGAGGGAGCGCTTCTCGCCTTTTGGCGACATCCAGGACATCTGGGTGGTGCGGGACAAG
CACACCAAGGAGTCCAAGGGCATTGCTTTCGTCAAGTTCGCCCGCAGCTCACAGGCCTGC
AGGGCCATGGAGGAGATGCATGGCCAGTGCCTCGGCCCCAACGACACCAAGCCCATCAAG
GTTTTCATTGCTCAGTCCCGATCATCTGGAAGTCACCGAGATGTTGAAGATGAAGAACTT
ACAAGAATCTTTGTTATGATACCAAAGTCCTACACAGAAGAAGATCTGCGGGAAAAATTT
AAGGTGTATGGAGATATCGAGTATTGCAGCATTATTAAGAATAAAGTGACTGGAGAAAGT
AAAGGTTTGGGCTACGTACGATACTTAAAACCATCACAAGCTGCCCAAGCAATAGAAAAC
TGTGATCGAAGTTTTAGAGCAATCTTGGCTGAACCTAAAAATAAAGCATCTGAATCCTCT
GAACAAGATTATTATAGTAATATGAGGCAAGAAGCTTTGGGACATGAACCTAGAGTAAAT
ATGTTTCCATTTGAACAACAATCTGAATTTTCAAGTTTTGACAAGAATGATAGCCGAGGC
CAGGAAGCAATCTCCAAACGCTTGTCAGTTGTATCAAGAGTTCCTTTCACTGAAGAACAG
CTTTTCAGCATTTTTGATATAGTACCAGGATTGGAATATTGTGAAGTTCAACGAGATCCT
TATTCAAATTATGGTCATGGAGTGGTTCAGTATTTTAATGTAGCATCAGCTATTTATGCA
AAATACAAATTACATGGATTTCAGTACCCTCCTGGGAACCGAATAGGTGTTTCCTTCATT
GATGATGGAAGTAATGCAACAGATCTCCTTAGAAAAATGGCAACACAGATGGTAGCTGCA
CAGCTTGCATCAATGGTGTGGAATAACCCAAGTCAGCAACAATTTATGCAATTTGGAGGA
AGCTCTGGATCACAGTTGCCTCAAATCCAGACAGATGTTGTACTTCCATCATGCAAAAAA
AAAGCTCCTGCTGAAACTCCTGTGAAAGAAAGACTTTTTATTGTGTTTAATCCTCATCCT
TTACCTTTAGACGTATTAGAAGATATATTCTGTCGTTTTGGTAACCTGATCGAAGTTTAC
CTTGTGTCAGGAAAAAATGTGGGGTATGCCAAGTATGCCGATAGAATAAGTGCTAATGAT
GCCATTGCCACTCTACATGGAAAGATTCTGAATGGGGTGAGACTTAAAGTTATGCTGGCA
GATTCGCCAAGAGAAGAATCTAACAAACGGCAAAGAACTTACTGC

Protein sequence predicted based on nucleotide sequence

MDEAGSSASGGGFRPGVDSLDEPPNSRIFLVISKYTPESVLRERFSPFGDIQDIWVVRDK
HTKESKGIAFVKFARSSQACRAMEEMHGQCLGPNDTKPIKVFIAQSRSSGSHRDVEDEEL
TRIFVMIPKSYTEEDLREKFKVYGDIEYCSIIKNKVTGESKGLGYVRYLKPSQAAQAIEN
CDRSFRAILAEPKNKASESSEQDYYSNMRQEALGHEPRVNMFPFEQQSEFSSFDKNDSRG
QEAISKRLSVVSRVPFTEEQLFSIFDIVPGLEYCEVQRDPYSNYGHGVVQYFNVASAIYA
KYKLHGFQYPPGNRIGVSFIDDGSNATDLLRKMATQMVAAQLASMVWNNPSQQQFMQFGG
SSGSQLPQIQTDVVLPSCKKKAPAETPVKERLFIVFNPHPLPLDVLEDIFCRFGNLIEVY
LVSGKNVGYAKYADRISANDAIATLHGKILNGVRLKVMLADSPREESNKRQRTYC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1517

Start on reference sequence 93

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

RBM45

Symbol Nomenclature :

RBM45

SYNONYM :

DRB1

Designation :

Full Nomenclature :

RNA binding motif protein 45

GENEID :

129831

HGNC :

24468

MIM :

608888

Vega :

OTTHUMG00000154202

Target GenBank:

BC066549.1

Reference Sequence Alignment

  

HsCD00288050      ATGGACGAAGCTGGCAGCTCTGCGAGCGGCGGGGGCTTCCGCCCGGGCGTGGACAGCCTG
NM_152945.2       ATGGACGAAGCTGGCAGCTCTGCGAGCGGCGGGGGCTTCCGCCCGGGCGTGGACAGCCTG
                  ************************************************************

HsCD00288050      GACGAACCGCCCAACAGCCGCATCTTCCTTGTGATCAGCAAGTACACACCTGAGTCGGTG
NM_152945.2       GACGAACCGCCCAACAGCCGCATCTTCCTTGTGATCAGCAAGTACACACCTGAGTCGGTG
                  ************************************************************

HsCD00288050      CTGAGGGAGCGCTTCTCGCCTTTTGGCGACATCCAGGACATCTGGGTGGTGCGGGACAAG
NM_152945.2       CTGAGGGAGCGCTTCTCGCCTTTTGGCGACATCCAGGACATCTGGGTGGTGCGGGACAAG
                  ************************************************************

HsCD00288050      CACACCAAGGAGTCCAAGGGCATTGCTTTCGTCAAGTTCGCCCGCAGCTCACAGGCCTGC
NM_152945.2       CACACCAAGGAGTCCAAGGGCATTGCTTTCGTCAAGTTCGCCCGCAGCTCACAGGCCTGC
                  ************************************************************

HsCD00288050      AGGGCCATGGAGGAGATGCATGGCCAGTGCCTCGGCCCCAACGACACCAAGCCCATCAAG
NM_152945.2       AGGGCCATGGAGGAGATGCATGGCCAGTGCCTCGGCCCCAACGACACCAAGCCCATCAAG
                  ************************************************************

HsCD00288050      GTTTTCATTGCTCAGTCCCGATCATCTGGAAGTCACCGAGATGTTGAAGATGAAGAACTT
NM_152945.2       GTTTTCATTGCTCAGTCCCGATCATCTGGAAGTCACCGAGATGTTGAAGATGAAGAACTT
                  ************************************************************

HsCD00288050      ACAAGAATCTTTGTTATGATACCAAAGTCCTACACAGAAGAAGATCTGCGGGAAAAATTT
NM_152945.2       ACAAGAATCTTTGTTATGATACCAAAGTCCTACACAGAAGAAGATCTGCGGGAAAAATTT
                  ************************************************************

HsCD00288050      AAGGTGTATGGAGATATCGAGTATTGCAGCATTATTAAGAATAAAGTGACTGGAGAAAGT
NM_152945.2       AAGGTGTATGGAGATATCGAGTATTGCAGCATTATTAAGAATAAAGTGACTGGAGAAAGT
                  ************************************************************

HsCD00288050      AAAGGTTTGGGCTACGTACGATACTTAAAACCATCACAAGCTGCCCAAGCAATAGAAAAC
NM_152945.2       AAAGGTTTGGGCTACGTACGATACTTAAAACCATCACAAGCTGCCCAAGCAATAGAAAAC
                  ************************************************************

HsCD00288050      TGTGATCGAAGTTTTAGAGCAATCTTGGCTGAACCTAAAAATAAAGCATCTGAATCCTCT
NM_152945.2       TGTGATCGAAGTTTTAGAGCAATCTTGGCTGAACCTAAAAATAAAGCATCTGAATCCTCT
                  ************************************************************

HsCD00288050      GAACAAGATTATTATAGTAATATGAGGCAAGAAGCTTTGGGACATGAACCTAGAGTAAAT
NM_152945.2       GAACAAGATTATTATAGTAATATGAGGCAAGAAGCTTTGGGACATGAACCTAGAGTAAAT
                  ************************************************************

HsCD00288050      ATGTTTCCATTTGAACAACAATCTGAATTTTCAAGTTTTGACAAGAATGATAGCCGAGGC
NM_152945.2       ATGTTTCCATTTGAACAACAATCTGAATTTTCAAGTTTTGACAAGAATGATAGCCGAGGC
                  ************************************************************

HsCD00288050      CAGGAAGCAATCTCCAAACGCTTGTCAGTTGTATCAAGAGTTCCTTTCACTGAAGAACAG
NM_152945.2       CAGGAAGCAATCTCCAAACGCTTGTCAGTTGTATCAAGAGTTCCTTTCACTGAAGAACAG
                  ************************************************************

HsCD00288050      CTTTTCAGCATTTTTGATATAGTACCAGGATTGGAATATTGTGAAGTTCAACGAGATCCT
NM_152945.2       CTTTTCAGCATTTTTGATATAGTACCAGGATTGGAATATTGTGAAGTTCAACGAGATCCT
                  ************************************************************

HsCD00288050      TATTCAAATTATGGTCATGGAGTGGTTCAGTATTTTAATGTAGCATCAGCTATTTATGCA
NM_152945.2       TATTCAAATTATGGTCATGGAGTGGTTCAGTATTTTAATGTAGCATCAGCTATTTATGCA
                  ************************************************************

HsCD00288050      AAATACAAATTACATGGATTTCAGTACCCTCCTGGGAACCGAATAGGTGTTTCCTTCATT
NM_152945.2       AAATACAAATTACATGGATTTCAGTACCCTCCTGGGAACCGAATAGGTGTTTCCTTCATT
                  ************************************************************

HsCD00288050      GATGATGGAAGTAATGCAACAGATCTCCTTAGAAAAATGGCAACACAGATGGTAGCTGCA
NM_152945.2       GATGATGGAAGTAATGCAACAGATCTCCTTAGAAAAATGGCAACACAGATGGTAGCTGCA
                  ************************************************************

HsCD00288050      CAGCTTGCATCAATGGTGTGGAATAACCCAAGTCAGCAACAATTTATGCAATTTGGAGGA
NM_152945.2       CAGCTTGCATCAATGGTGTGGAATAACCCAAGTCAGCAACAATTTATGCAATTTGGAGGA
                  ************************************************************

HsCD00288050      AGCTCTGGATCACAGTTGCCTCAAATCCAGACAGATGTTGTACTTCCATCATGCAAAAAA
NM_152945.2       AGCTCTGGATCACAGTTGCCTCAAATCCAGACAGATGTTGTACTTCCATCATGCAAAAAA
                  ************************************************************

HsCD00288050      AAAGCTCCTGCTGAAACTCCTGTGAAAGAAAGACTTTTTATTGTGTTTAATCCTCATCCT
NM_152945.2       AAAGCTCCTGCTGAAACTCCTGTGAAAGAAAGACTTTTTATTGTGTTTAATCCTCATCCT
                  ************************************************************

HsCD00288050      TTACCTTTAGACGTATTAGAAGATATATTCTGTCGTTTTGGTAACCTGATCGAAGTTTAC
NM_152945.2       TTACCTTTAGACGTATTAGAAGATATATTCTGTCGTTTTGGTAACCTGATCGAAGTTTAC
                  ************************************************************

HsCD00288050      CTTGTGTCAGGAAAAAATGTGGGGTATGCCAAGTATGCCGATAGAATAAGTGCTAATGAT
NM_152945.2       CTTGTGTCAGGAAAAAATGTGGGGTATGCCAAGTATGCCGATAGAATAAGTGCTAATGAT
                  ************************************************************

HsCD00288050      GCCATTGCCACTCTACATGGAAAGATTCTGAATGGGGTGAGACTTAAAGTTATGCTGGCA
NM_152945.2       GCCATTGCCACTCTACATGGAAAGATTCTGAATGGGGTGAGACTTAAAGTTATGCTGGCA
                  ************************************************************

HsCD00288050      GATTCGCCAAGAGAAGAATCTAACAAACGGCAAAGAACTTACTGC
NM_152945.2       GATTCGCCAAGAGAAGAATCTAACAAACGGCAAAGAACTTACTGA
                  ********************************************.