Gene:
Gene Symbol:
RASSF8
Gene Name:
Ras association (RalGDS/AF-6) domain family (N-terminal) member 8
Original Clone ID:
12933
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00288424
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAACTTAAAGTATGGGTGGATGGAGTTCAGAGGATTGTTTGTGGAGTCACTGAAGTC
ACAACTTGCCAGGAGGTTGTCATAGCCTTAGCTCAAGCAATAGGTCGAACTGGAAGGTAC
ACCCTTATAGAGAAATGGAGAGATACTGAAAGACACTTAGCACCTCATGAAAATCCTATC
ATATCCTTAAACAAATGGGGGCAGTATGCTAGTGATGTGCAGCTCATTCTACGACGAACT
GGGCCGTCTCTCAGTGAGCGACCCACTTCAGACAGTGTGGCTCGAATTCCTGAAAGAACT
TTATACAGGCAGAGTCTGCCCCCCTTAGCTAAACTGAGGCCTCAGATTGACAAATCAATC
AAAAGGAGGGAACCGAAAAGGAAATCACTGACATTTACAGGAGGTGCCAAAGGATTAATG
GACATTTTTGGAAAAGGTAAAGAAACTGAGTTTAAGCAAAAGGTGCTGAATAACTGCAAA
ACAACAGCAGATGAGTTGAAGAAGCTAATCCGTCTGCAGACAGAGAAGCTTCAATCCATT
GAGAAACAGCTGGAATCTAATGAAATAGAAATAAGATTTTGGGAGCAAAAGTATAATTCC
AACCTTGAAGAGGAAATTGTCCGTCTAGAGCAAAAGATCAAAAGAAACGATGTAGAAATT
GAGGAGGAAGAATTCTGGGAAAATGAATTACAGATTGAACAGGAAAATGAAAAACAGCTG
AAGGATCAACTTCAAGAAATAAGACAGAAAATAACAGAATGTGAAAACAAATTAAAGGAC
TATTTGGCACAGATCCGGACTATGGAAAGTGGTCTTGAAGCAGAAAAATTGCAACGGGAA
GTTCAAGAGGCACAGGTCAATGAGGAAGAGGTTAAAGGAAAGATCGGTAAGGTCAAAGGG
GAGATTGACATTCAAGGCCAGCAGAGTCTGAGGTTGGAAAATGGCATCAAAGCTGTGGAA
AGATCTCTTGGACAAGCCACCAAACGCTTACAGGACAAAGAACAGGAACTGGAGCAGTTG
ACTAAGGAGTTGCGGCAAGTCAATCTCCAGCAGTTCATCCAGCAGACAGGGACAAAAGTT
ACCGTTTTGCCAGCGGAGCCCATTGAAATAGAGGCCTCACATGCAGACATTGAAAGGGGG
ATCATCATTCTTTCTGATAAGCAGGAGTGTAAAGATTAC
Protein sequence predicted based on nucleotide sequence
MELKVWVDGVQRIVCGVTEVTTCQEVVIALAQAIGRTGRYTLIEKWRDTERHLAPHENPI ISLNKWGQYASDVQLILRRTGPSLSERPTSDSVARIPERTLYRQSLPPLAKLRPQIDKSI KRREPKRKSLTFTGGAKGLMDIFGKGKETEFKQKVLNNCKTTADELKKLIRLQTEKLQSI EKQLESNEIEIRFWEQKYNSNLEEEIVRLEQKIKRNDVEIEEEEFWENELQIEQENEKQL KDQLQEIRQKITECENKLKDYLAQIRTMESGLEAEKLQREVQEAQVNEEEVKGKIGKVKG EIDIQGQQSLRLENGIKAVERSLGQATKRLQDKEQELEQLTKELRQVNLQQFIQQTGTKV TVLPAEPIEIEASHADIERGIIILSDKQECKDY
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
1501
Start on reference sequence
323
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223
Synonyms:
pDONR223, pENTR223-XnCDSYn
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.
Size (bp):
2815
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Mark Vidal
Publications:
PMID:
15489335
Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
bacterial origin of replication
2220
2753
gene fragment
pas
5p stem of pBR primose assembly site
63
73
gene fragment
pas prime
3p stem of pBR pas(L)
105
116
recombination site
attL1
attL1 recombination site
570
665
recombination site
attL2
attL2 recombination site
793
696
selectable marker
SpnR
spectinomycin resistance gene
1062
2072
trxn termination sequence
T1-T2
rrnBT1 and T2 terminators
510
224
