DNASU Plasmid | GNAL (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GNAL

Gene Name: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type

Original Clone ID:

25793

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288581

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1377nts

Open reading frame: 1 to 1377

Reference Sequence Alignment

ATGGGTCTGTGCTACAGTCTGCGGCCGCTGCTTTTCGGGGGCCCAGGGGACGACCCCTGC
GCGGCCTCGGAGCCGCCGGTGGAGGACGCGCAGCCCGCCCCGGCCCCGGCCCTGGCCCCA
GTCCGGGCGGCCGCAAGGGACACGGCCCGGACCCTGCTCCCTCGGGGCGGCGAAGGGAGC
CCGGCATGCGCTCGGCCCAAAGCAGACAAGCCGAAGGAGAAGCGGCAGCGCACCGAGCAG
CTTAGTGCCGAGGAGCGCGAGGCGGCCAAGGAGCGCGAGGCGGTCAAGGAGGCGAGGAAA
GTGAGCCGGGGCATCGACCGCATGCTGCGCGACCAGAAGCGCGACCTGCAGCAGACGCAC
CGGCTCCTGCTGCTCGGGGCTGGTGAGTCTGGGAAAAGCACTATCGTCAAACAGATGAGG
ATCCTGCACGTCAATGGGTTTAATCCCGAGGAAAAGAAACAGAAAATTCTGGACATCCGG
AAAAATGTTAAAGATGCTATCGTGACAATTGTTTCAGCAATGAGTACTATAATACCTCCA
GTTCCGCTGGCCAACCCTGAAAACCAATTTCGATCAGACTACATCAAGAGCATAGCCCCT
ATCACTGACTTTGAATATTCCCAGGAATTCTTTGACCATGTGAAAAAACTTTGGGACGAT
GAAGGCGTGAAGGCATGCTTTGAGAGATCCAACGAATACCAGCTGATTGACTGTGCACAA
TACTTCCTGGAAAGAATCGACAGCGTCAGCTTGGTTGACTACACACCCACAGACCAGGAC
CTCCTCAGATGCAGAGTTCTGACATCTGGGATTTTTGAGACACGATTCCAAGTGGACAAA
GTAAACTTCCACATGTTTGATGTTGGTGGCCAGAGGGATGAGAGGAGAAAATGGATCCAG
TGCTTTAACGATGTCACAGCTATCATTTACGTCGCAGCCTGCAGTAGCTACAACATGGTG
ATTCGAGAAGATAACAACACCAACAGGCTGAGAGAGTCCCTGGATCTTTTTGAAAGCATC
TGGAACAACAGGTGGTTACGGACCATTTCTATCATCTTGTTCTTGAACAAACAAGATATG
CTGGCAGAAAAAGTCTTGGCAGGGAAATCAAAAATTGAAGACTATTTCCCAGAATATGCA
AATTATACTGTTCCTGAAGACGCAACACCAGATGCAGGAGAAGATCCCAAAGTTACAAGA
GCCAAGTTCTTTATCCGGGACCTGTTTTTGAGGATCAGCACGGCCACCGGTGACGGCAAA
CATTACTGCTACCCGCACTTCACCTGCGCCGTGGACACAGAGAACATCCGCAGGGTGTTC
AACGACTGCCGCGACATCATCCAGCGGATGCACCTCAAGCAGTATGAGCTCTTGTGC

Protein sequence predicted based on nucleotide sequence

MGLCYSLRPLLFGGPGDDPCAASEPPVEDAQPAPAPALAPVRAAARDTARTLLPRGGEGS
PACARPKADKPKEKRQRTEQLSAEEREAAKEREAVKEARKVSRGIDRMLRDQKRDLQQTH
RLLLLGAGESGKSTIVKQMRILHVNGFNPEEKKQKILDIRKNVKDAIVTIVSAMSTIIPP
VPLANPENQFRSDYIKSIAPITDFEYSQEFFDHVKKLWDDEGVKACFERSNEYQLIDCAQ
YFLERIDSVSLVDYTPTDQDLLRCRVLTSGIFETRFQVDKVNFHMFDVGGQRDERRKWIQ
CFNDVTAIIYVAACSSYNMVIREDNNTNRLRESLDLFESIWNNRWLRTISIILFLNKQDM
LAEKVLAGKSKIEDYFPEYANYTVPEDATPDAGEDPKVTRAKFFIRDLFLRISTATGDGK
HYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLKQYELLC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1677

Start on reference sequence 301

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

GNAL

Symbol Nomenclature :

GNAL

SYNONYM :

DYT25

Designation :

adenylate cyclase-stimulating G alpha protein, olfactory type|guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide, olfactory type|guanine nucleotide-binding protein G(olf) subunit alpha

Full Nomenclature :

guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type

GENEID :

2774

HGNC :

4388

MIM :

139312

Vega :

OTTHUMG00000131660

Target GenBank:

BC050021.1

Reference Sequence Alignment

  

HsCD00288581      ATGGGTCTGTGCTACAGTCTGCGGCCGCTGCTTTTCGGGGGCCCAGGGGACGACCCCTGC
NM_182978.3       ATGGGTCTGTGCTACAGTCTGCGGCCGCTGCTTTTCGGGGGCCCAGGGGACGACCCCTGC
                  ************************************************************

HsCD00288581      GCGGCCTCGGAGCCGCCGGTGGAGGACGCGCAGCCCGCCCCGGCCCCGGCCCTGGCCCCA
NM_182978.3       GCGGCCTCGGAGCCGCCGGTGGAGGACGCGCAGCCCGCCCCGGCCCCGGCCCTGGCCCCA
                  ************************************************************

HsCD00288581      GTCCGGGCGGCCGCAAGGGACACGGCCCGGACCCTGCTCCCTCGGGGCGGCGAAGGGAGC
NM_182978.3       GTCCGGGCGGCCGCAAGGGACACGGCCCGGACCCTGCTCCCTCGGGGCGGCGAAGGGAGC
                  ************************************************************

HsCD00288581      CCGGCATGCGCTCGGCCCAAAGCAGACAAGCCGAAGGAGAAGCGGCAGCGCACCGAGCAG
NM_182978.3       CCGGCATGCGCTCGGCCCAAAGCAGACAAGCCGAAGGAGAAGCGGCAGCGCACCGAGCAG
                  ************************************************************

HsCD00288581      CTTAGTGCCGAGGAGCGCGAGGCGGCCAAGGAGCGCGAGGCGGTCAAGGAGGCGAGGAAA
NM_182978.3       CTGAGTGCCGAGGAGCGCGAGGCGGCCAAGGAGCGCGAGGCGGTCAAGGAGGCGAGGAAA
                  ** *********************************************************

HsCD00288581      GTGAGCCGGGGCATCGACCGCATGCTGCGCGACCAGAAGCGCGACCTGCAGCAGACGCAC
NM_182978.3       GTGAGCCGGGGCATCGACCGCATGCTGCGCGACCAGAAGCGCGACCTGCAGCAGACGCAC
                  ************************************************************

HsCD00288581      CGGCTCCTGCTGCTCGGGGCTGGTGAGTCTGGGAAAAGCACTATCGTCAAACAGATGAGG
NM_182978.3       CGGCTCCTGCTGCTCGGGGCTGGTGAGTCTGGGAAAAGCACTATCGTCAAACAGATGAGG
                  ************************************************************

HsCD00288581      ATCCTGCACGTCAATGGGTTTAATCCCGAGGAAAAGAAACAGAAAATTCTGGACATCCGG
NM_182978.3       ATCCTGCACGTCAATGGGTTTAATCCCGAGGAAAAGAAACAGAAAATTCTGGACATCCGG
                  ************************************************************

HsCD00288581      AAAAATGTTAAAGATGCTATCGTGACAATTGTTTCAGCAATGAGTACTATAATACCTCCA
NM_182978.3       AAAAATGTTAAAGATGCTATCGTGACAATTGTTTCAGCAATGAGTACTATAATACCTCCA
                  ************************************************************

HsCD00288581      GTTCCGCTGGCCAACCCTGAAAACCAATTTCGATCAGACTACATCAAGAGCATAGCCCCT
NM_182978.3       GTTCCGCTGGCCAACCCTGAAAACCAATTTCGATCAGACTACATCAAGAGCATAGCCCCT
                  ************************************************************

HsCD00288581      ATCACTGACTTTGAATATTCCCAGGAATTCTTTGACCATGTGAAAAAACTTTGGGACGAT
NM_182978.3       ATCACTGACTTTGAATATTCCCAGGAATTCTTTGACCATGTGAAAAAACTTTGGGACGAT
                  ************************************************************

HsCD00288581      GAAGGCGTGAAGGCATGCTTTGAGAGATCCAACGAATACCAGCTGATTGACTGTGCACAA
NM_182978.3       GAAGGCGTGAAGGCATGCTTTGAGAGATCCAACGAATACCAGCTGATTGACTGTGCACAA
                  ************************************************************

HsCD00288581      TACTTCCTGGAAAGAATCGACAGCGTCAGCTTGGTTGACTACACACCCACAGACCAGGAC
NM_182978.3       TACTTCCTGGAAAGAATCGACAGCGTCAGCTTGGTTGACTACACACCCACAGACCAGGAC
                  ************************************************************

HsCD00288581      CTCCTCAGATGCAGAGTTCTGACATCTGGGATTTTTGAGACACGATTCCAAGTGGACAAA
NM_182978.3       CTCCTCAGATGCAGAGTTCTGACATCTGGGATTTTTGAGACACGATTCCAAGTGGACAAA
                  ************************************************************

HsCD00288581      GTAAACTTCCACATGTTTGATGTTGGTGGCCAGAGGGATGAGAGGAGAAAATGGATCCAG
NM_182978.3       GTAAACTTCCACATGTTTGATGTTGGTGGCCAGAGGGATGAGAGGAGAAAATGGATCCAG
                  ************************************************************

HsCD00288581      TGCTTTAACGATGTCACAGCTATCATTTACGTCGCAGCCTGCAGTAGCTACAACATGGTG
NM_182978.3       TGCTTTAACGATGTCACAGCTATCATTTACGTCGCAGCCTGCAGTAGCTACAACATGGTG
                  ************************************************************

HsCD00288581      ATTCGAGAAGATAACAACACCAACAGGCTGAGAGAGTCCCTGGATCTTTTTGAAAGCATC
NM_182978.3       ATTCGAGAAGATAACAACACCAACAGGCTGAGAGAGTCCCTGGATCTTTTTGAAAGCATC
                  ************************************************************

HsCD00288581      TGGAACAACAGGTGGTTACGGACCATTTCTATCATCTTGTTCTTGAACAAACAAGATATG
NM_182978.3       TGGAACAACAGGTGGTTACGGACCATTTCTATCATCTTGTTCTTGAACAAACAAGATATG
                  ************************************************************

HsCD00288581      CTGGCAGAAAAAGTCTTGGCAGGGAAATCAAAAATTGAAGACTATTTCCCAGAATATGCA
NM_182978.3       CTGGCAGAAAAAGTCTTGGCAGGGAAATCAAAAATTGAAGACTATTTCCCAGAATATGCA
                  ************************************************************

HsCD00288581      AATTATACTGTTCCTGAAGACGCAACACCAGATGCAGGAGAAGATCCCAAAGTTACAAGA
NM_182978.3       AATTATACTGTTCCTGAAGACGCAACACCAGATGCAGGAGAAGATCCCAAAGTTACAAGA
                  ************************************************************

HsCD00288581      GCCAAGTTCTTTATCCGGGACCTGTTTTTGAGGATCAGCACGGCCACCGGTGACGGCAAA
NM_182978.3       GCCAAGTTCTTTATCCGGGACCTGTTTTTGAGGATCAGCACGGCCACCGGTGACGGCAAA
                  ************************************************************

HsCD00288581      CATTACTGCTACCCGCACTTCACCTGCGCCGTGGACACAGAGAACATCCGCAGGGTGTTC
NM_182978.3       CATTACTGCTACCCGCACTTCACCTGCGCCGTGGACACAGAGAACATCCGCAGGGTGTTC
                  ************************************************************

HsCD00288581      AACGACTGCCGCGACATCATCCAGCGGATGCACCTCAAGCAGTATGAGCTCTTGTGC
NM_182978.3       AACGACTGCCGCGACATCATCCAGCGGATGCACCTCAAGCAGTATGAGCTCTTGTGA
                  ********************************************************.

Pathways

NCI :

Endothelins

NCI :

LPA4-mediated signaling events

NCI :

Plasma membrane estrogen receptor signaling

NCI :

Rapid glucocorticoid signaling

Panther :

Cortocotropin releasing factor receptor signaling pathway

Panther :

Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway

Reactome :

Activation of GABAB receptors

Reactome :

Adenylate cyclase activating pathway

Reactome :

Adenylate cyclase inhibitory pathway

Reactome :

G-protein mediated events

Reactome :

GABA B receptor activation

Reactome :

GABA receptor activation

Reactome :

GPCR downstream signaling

Reactome :

Inhibition of adenylate cyclase pathway

Reactome :

Neuronal System

Reactome :

Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell

Reactome :

Olfactory Signaling Pathway

Reactome :

Opioid Signalling

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

Reactome :

Transmission across Chemical Synapses

WikiPathway :

G Protein Signaling Pathways

Protein

SMART domain :

G_alpha : G protein alpha subunit

UniProt :

P38405

UniProt :

Q86XU3

UniProt :

Q8N2B4

UniProt :

A8K1Y9

HPRD :

00758

Clone

Cloning Information :

471818

HIP Master Clone ID :

394736

Original Clone ID :

25793

Organism

Species Specific ID:

2774

Genome

Chromosome :

Map Location :

18p11.22-p11.21

Ensembl :

ENSG00000141404

Reagents

Labome :

GNAL-antibody