DNASU Plasmid | DDX49 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: DDX49

Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 49

Original Clone ID:

4356

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288846

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1452nts

Open reading frame: 1 to 1452

Reference Sequence Alignment

ATGGCAGGCTTCGCGGAGCTCGGGCTGTCATCGTGGCTCGTGGAACAATGTCGGCAGCTG
GGTTTGAAGCAGCCCACGCCCGTGCAGCTCGGCTGCATCCCCGCCATCCTGGAGGGTCGA
GACTGCTTGGGCTGTGCTAAGACAGGCAGTGGGAAGACAGCAGCGTTTGTCCTTCCCATC
TTGCAGAAGCTGTCTGAGGATCCCTATGGCATCTTCTGCCTCGTCCTGACACCCACCAGG
GAGCTGGCCTACCAGATCGCAGAGCAGTTCCGGGTCCTGGGGAAGCCTCTAGGGCTGAAA
GACTGCATCATCGTCGGTGGCATGGACATGGTGGCCCAGGCGCTGGAGCTCTCTCGGAAA
CCACACGTGGTCATCGCCACGCCGGGGCGCCTGGCAGATCACCTGCGCAGCTCCAACACT
TTTAGTATAAAGAAGATCCGCTTCCTGGTGATGGATGAGGCAGACCGGCTGCTGGAACAG
GGCTGCACTGACTTCACCGTGGACCTGGAGGCCATCCTGGCGGCTGTGCCGGCCCGCAGG
CAGACACTGCTGTTCAGCGCCACGCTGACCGACACACTCCGGGAGCTGCAGGGTCTGGCC
ACCAACCAGCCCTTCTTCTGGGAAGCACAGGCCCCGGTGAGCACCGTGGAGCAGCTGGAC
CAGCGCTACCTGCTGGTGCCTGAGAAGGTCAAGGACGCCTACCTGGTCCACCTGATCCAG
CGCTTCCAGGATGAGCACGAGGACTGGTCCATTATCATCTTCACCAACACGTGCAAGACC
TGCCAGATTCTGTGCATGATGCTGCGCAAATTCAGCTTCCCCACCGTGGCTCTGCACTCC
ATGATGAAGCAGAAAGAACGCTTTGCCGCCCTAGCCAAGTTCAAGTCCAGCATCTACCGG
ATCCTGATCGCAACAGACGTGGCCTCCCGGGGCCTGGACATCCCTACGGTACAGGTGGTC
ATCAACCACAACACCCCCGGGCTCCCCAAGATCTACATCCACCGAGTCGGCCGGACGGCC
CGTGCAGGGCGGCAGGGTCAGGCCATCACGCTGGTGACACAGTACGACATCCACCTGGTG
CACGCCATCGAGGAGCAGATCAAGAAGAAGCTGGAGGAGTTCTCCGTGGAAGAGGCCGAG
GTGCTACAGATCCTCACACAGGTCAACGTGGTGCGAAGAGAGTGTGAGATCAAACTGGAG
GCGGCCCACTTTGACGAAAAGAAGGAGATCAACAAACGGAAGCAGCTGATCCTGGAGGGG
AAGGACCCTGACCTGGAGGCCAAGCGCAAGGCTGAGCTGGCCAAGATCAAGCAGAAGAAC
CGGCGCTTCAAGGAGAAGGTGGAGGAGACGCTGAAGCGACAGAAGGCTGGCAGGGCTGGC
CACAAGGGGCGTCCACCCAGGACACCGTCTGGGTCCCACTCAGGCCCAGTCCCCTCCCAG
GGCCTGGTCTGC

Protein sequence predicted based on nucleotide sequence

MAGFAELGLSSWLVEQCRQLGLKQPTPVQLGCIPAILEGRDCLGCAKTGSGKTAAFVLPI
LQKLSEDPYGIFCLVLTPTRELAYQIAEQFRVLGKPLGLKDCIIVGGMDMVAQALELSRK
PHVVIATPGRLADHLRSSNTFSIKKIRFLVMDEADRLLEQGCTDFTVDLEAILAAVPARR
QTLLFSATLTDTLRELQGLATNQPFFWEAQAPVSTVEQLDQRYLLVPEKVKDAYLVHLIQ
RFQDEHEDWSIIIFTNTCKTCQILCMMLRKFSFPTVALHSMMKQKERFAALAKFKSSIYR
ILIATDVASRGLDIPTVQVVINHNTPGLPKIYIHRVGRTARAGRQGQAITLVTQYDIHLV
HAIEEQIKKKLEEFSVEEAEVLQILTQVNVVRRECEIKLEAAHFDEKKEINKRKQLILEG
KDPDLEAKRKAELAKIKQKNRRFKEKVEETLKRQKAGRAGHKGRPPRTPSGSHSGPVPSQ
GLVC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1509

Start on reference sequence 58

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

DDX49

Symbol Nomenclature :

DDX49

SYNONYM :

R27090_2

Designation :

DEAD box protein 49|probable ATP-dependent RNA helicase DDX49

Full Nomenclature :

DEAD (Asp-Glu-Ala-Asp) box polypeptide 49

GENEID :

54555

HGNC :

18684

Vega :

OTTHUMG00000182994

Target GenBank:

BC002674.2

Reference Sequence Alignment

  

HsCD00288846      ATGGCAGGCTTCGCGGAGCTCGGGCTGTCATCGTGGCTCGTGGAACAATGTCGGCAGCTG
NM_019070.4       ATGGCAGGCTTCGCGGAGCTCGGGCTGTCATCGTGGCTCGTGGAACAATGTCGGCAGCTG
                  ************************************************************

HsCD00288846      GGTTTGAAGCAGCCCACGCCCGTGCAGCTCGGCTGCATCCCCGCCATCCTGGAGGGTCGA
NM_019070.4       GGTTTGAAGCAGCCCACGCCCGTGCAGCTCGGCTGCATCCCCGCCATCCTGGAGGGTCGA
                  ************************************************************

HsCD00288846      GACTGCTTGGGCTGTGCTAAGACAGGCAGTGGGAAGACAGCAGCGTTTGTCCTTCCCATC
NM_019070.4       GACTGCTTGGGCTGTGCTAAGACAGGCAGTGGGAAGACAGCAGCGTTTGTCCTTCCCATC
                  ************************************************************

HsCD00288846      TTGCAGAAGCTGTCTGAGGATCCCTATGGCATCTTCTGCCTCGTCCTGACACCCACCAGG
NM_019070.4       TTGCAGAAGCTGTCTGAGGATCCCTATGGCATCTTCTGCCTCGTCCTGACACCCACCAGG
                  ************************************************************

HsCD00288846      GAGCTGGCCTACCAGATCGCAGAGCAGTTCCGGGTCCTGGGGAAGCCTCTAGGGCTGAAA
NM_019070.4       GAGCTGGCCTACCAGATCGCAGAGCAGTTCCGGGTCCTGGGGAAGCCTCTAGGGCTGAAA
                  ************************************************************

HsCD00288846      GACTGCATCATCGTCGGTGGCATGGACATGGTGGCCCAGGCGCTGGAGCTCTCTCGGAAA
NM_019070.4       GACTGCATCATCGTCGGTGGCATGGACATGGTGGCCCAGGCGCTGGAGCTCTCTCGGAAA
                  ************************************************************

HsCD00288846      CCACACGTGGTCATCGCCACGCCGGGGCGCCTGGCAGATCACCTGCGCAGCTCCAACACT
NM_019070.4       CCACACGTGGTCATCGCCACGCCGGGGCGCCTGGCAGATCACCTGCGCAGCTCCAACACT
                  ************************************************************

HsCD00288846      TTTAGTATAAAGAAGATCCGCTTCCTGGTGATGGATGAGGCAGACCGGCTGCTGGAACAG
NM_019070.4       TTTAGTATAAAGAAGATCCGCTTCCTGGTGATGGATGAGGCAGACCGGCTGCTGGAACAG
                  ************************************************************

HsCD00288846      GGCTGCACTGACTTCACCGTGGACCTGGAGGCCATCCTGGCGGCTGTGCCGGCCCGCAGG
NM_019070.4       GGCTGCACTGACTTCACCGTGGACCTGGAGGCCATCCTGGCGGCTGTGCCGGCCCGCAGG
                  ************************************************************

HsCD00288846      CAGACACTGCTGTTCAGCGCCACGCTGACCGACACACTCCGGGAGCTGCAGGGTCTGGCC
NM_019070.4       CAGACACTGCTGTTCAGCGCCACGCTGACCGACACACTCCGGGAGCTGCAGGGTCTGGCC
                  ************************************************************

HsCD00288846      ACCAACCAGCCCTTCTTCTGGGAAGCACAGGCCCCGGTGAGCACCGTGGAGCAGCTGGAC
NM_019070.4       ACCAACCAGCCCTTCTTCTGGGAAGCACAGGCCCCGGTGAGCACCGTGGAGCAGCTGGAC
                  ************************************************************

HsCD00288846      CAGCGCTACCTGCTGGTGCCTGAGAAGGTCAAGGACGCCTACCTGGTCCACCTGATCCAG
NM_019070.4       CAGCGCTACCTGCTGGTGCCTGAGAAGGTCAAGGACGCCTACCTGGTCCACCTGATCCAG
                  ************************************************************

HsCD00288846      CGCTTCCAGGATGAGCACGAGGACTGGTCCATTATCATCTTCACCAACACGTGCAAGACC
NM_019070.4       CGCTTCCAGGATGAGCACGAGGACTGGTCCATTATCATCTTCACCAACACGTGCAAGACC
                  ************************************************************

HsCD00288846      TGCCAGATTCTGTGCATGATGCTGCGCAAATTCAGCTTCCCCACCGTGGCTCTGCACTCC
NM_019070.4       TGCCAGATTCTGTGCATGATGCTGCGCAAATTCAGCTTCCCCACCGTGGCTCTGCACTCC
                  ************************************************************

HsCD00288846      ATGATGAAGCAGAAAGAACGCTTTGCCGCCCTAGCCAAGTTCAAGTCCAGCATCTACCGG
NM_019070.4       ATGATGAAGCAGAAAGAACGCTTTGCCGCCCTAGCCAAGTTCAAGTCCAGCATCTACCGG
                  ************************************************************

HsCD00288846      ATCCTGATCGCAACAGACGTGGCCTCCCGGGGCCTGGACATCCCTACGGTACAGGTGGTC
NM_019070.4       ATCCTGATCGCAACAGACGTGGCCTCCCGGGGCCTGGACATCCCTACGGTACAGGTGGTC
                  ************************************************************

HsCD00288846      ATCAACCACAACACCCCCGGGCTCCCCAAGATCTACATCCACCGAGTCGGCCGGACGGCC
NM_019070.4       ATCAACCACAACACCCCCGGGCTCCCCAAGATCTACATCCACCGAGTCGGCCGGACGGCC
                  ************************************************************

HsCD00288846      CGTGCAGGGCGGCAGGGTCAGGCCATCACGCTGGTGACACAGTACGACATCCACCTGGTG
NM_019070.4       CGTGCAGGGCGGCAGGGTCAGGCCATCACGCTGGTGACACAGTACGACATCCACCTGGTG
                  ************************************************************

HsCD00288846      CACGCCATCGAGGAGCAGATCAAGAAGAAGCTGGAGGAGTTCTCCGTGGAAGAGGCCGAG
NM_019070.4       CACGCCATCGAGGAGCAGATCAAGAAGAAGCTGGAGGAGTTCTCCGTGGAAGAGGCCGAG
                  ************************************************************

HsCD00288846      GTGCTACAGATCCTCACACAGGTCAACGTGGTGCGAAGAGAGTGTGAGATCAAACTGGAG
NM_019070.4       GTGCTACAGATCCTCACACAGGTCAACGTGGTGCGAAGAGAGTGTGAGATCAAACTGGAG
                  ************************************************************

HsCD00288846      GCGGCCCACTTTGACGAAAAGAAGGAGATCAACAAACGGAAGCAGCTGATCCTGGAGGGG
NM_019070.4       GCGGCCCACTTTGACGAAAAGAAGGAGATCAACAAACGGAAGCAGCTGATCCTGGAGGGG
                  ************************************************************

HsCD00288846      AAGGACCCTGACCTGGAGGCCAAGCGCAAGGCTGAGCTGGCCAAGATCAAGCAGAAGAAC
NM_019070.4       AAGGACCCTGACCTGGAGGCCAAGCGCAAGGCTGAGCTGGCCAAGATCAAGCAGAAGAAC
                  ************************************************************

HsCD00288846      CGGCGCTTCAAGGAGAAGGTGGAGGAGACGCTGAAGCGACAGAAGGCTGGCAGGGCTGGC
NM_019070.4       CGGCGCTTCAAGGAGAAGGTGGAGGAGACGCTGAAGCGACAGAAGGCTGGCAGGGCTGGC
                  ************************************************************

HsCD00288846      CACAAGGGGCGTCCACCCAGGACACCGTCTGGGTCCCACTCAGGCCCAGTCCCCTCCCAG
NM_019070.4       CACAAGGGGCGTCCACCCAGGACACCGTCTGGGTCCCACTCAGGCCCAGTCCCCTCCCAG
                  ************************************************************

HsCD00288846      GGCCTGGTCTGC
NM_019070.4       GGCCTGGTCTGA
                  ***********.