DNASU Plasmid | OSTF1 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: OSTF1

Gene Name: osteoclast stimulating factor 1

Original Clone ID:

19907

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288876

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 654nts

Open reading frame: 1 to 654

Reference Sequence Alignment

ATGTCGAAGCCGCCACCCAAACCAGTCAAACCAGGTGAGGGAGGGCAAGTTAAAGTCTTC
AGAGCCCTGTATACGTTTGAACCCAGAACTCCAGATGAATTATACTTTGAGGAAGGTGAT
ATTATCTACATTACTGACATGAGCGATACCAATTGGTGGAAAGGCACCTCCAAAGGCAGG
ACTGGACTAATTCCAAGCAACTATGTGGCTGAGCAGGCAGAATCCATTGACAATCCATTG
CATGAAGCAGCAAAAAGAGGCAACTTGAGCTGGTTGAGAGAGTGTTTGGACAACAGAGTG
GGTGTTAATGGCTTAGACAAAGCTGGAAGCACTGCCTTATACTGGGCTTGCCACGGGGGC
CACAAAGATATAGTGGAAATGCTATTTACTCAACCAAATATTGAACTGAACCAGCAGAAC
AAGTTGGGAGATACAGCTTTGCATGCTGCTGCCTGGAAGGGTTATGCAGATATCGTCCAG
TTGTTTCTGGCAAAAGGTGCTAGAACAGACTTAAGAAACATTGAGAAGAAGCTGGCCTTC
GACATGGCTACCAATGCTGCCTGTGCATCTCTCCTGAAAAAGAAACAGGGAACAGATGCA
GTTCGAACATTAAGCAATGCCGAGGACTATCTCGATGATGAAGACTCAGATTAC

Protein sequence predicted based on nucleotide sequence

MSKPPPKPVKPGEGGQVKVFRALYTFEPRTPDELYFEEGDIIYITDMSDTNWWKGTSKGR
TGLIPSNYVAEQAESIDNPLHEAAKRGNLSWLRECLDNRVGVNGLDKAGSTALYWACHGG
HKDIVEMLFTQPNIELNQQNKLGDTALHAAAWKGYADIVQLFLAKGARTDLRNIEKKLAF
DMATNAACASLLKKKQGTDAVRTLSNAEDYLDDEDSDY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 109

End on reference sequence 762

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

OSTF1

Symbol Nomenclature :

OSTF1

SYNONYM :

OSF; SH3P2; bA235O14.1

Designation :

osteoclast-stimulating factor 1

Full Nomenclature :

osteoclast stimulating factor 1

GENEID :

26578

HGNC :

8510

MIM :

610180

Vega :

OTTHUMG00000020033

Target GenBank:

BC007459.1

Reference Sequence Alignment

  

HsCD00288876      ATGTCGAAGCCGCCACCCAAACCAGTCAAACCAGGTGAGGGAGGGCAAGTTAAAGTCTTC
NM_012383.4       ATGTCGAAGCCGCCACCCAAACCAGTCAAACCAG---------GGCAAGTTAAAGTCTTC
                  **********************************         *****************

HsCD00288876      AGAGCCCTGTATACGTTTGAACCCAGAACTCCAGATGAATTATACTTTGAGGAAGGTGAT
NM_012383.4       AGAGCCCTGTATACGTTTGAACCCAGAACTCCAGATGAATTATACTTTGAGGAAGGTGAT
                  ************************************************************

HsCD00288876      ATTATCTACATTACTGACATGAGCGATACCAATTGGTGGAAAGGCACCTCCAAAGGCAGG
NM_012383.4       ATTATCTACATTACTGACATGAGCGATACCAATTGGTGGAAAGGCACCTCCAAAGGCAGG
                  ************************************************************

HsCD00288876      ACTGGACTAATTCCAAGCAACTATGTGGCTGAGCAGGCAGAATCCATTGACAATCCATTG
NM_012383.4       ACTGGACTAATTCCAAGCAACTATGTGGCTGAGCAGGCAGAATCCATTGACAATCCATTG
                  ************************************************************

HsCD00288876      CATGAAGCAGCAAAAAGAGGCAACTTGAGCTGGTTGAGAGAGTGTTTGGACAACAGAGTG
NM_012383.4       CATGAAGCAGCAAAAAGAGGCAACTTGAGCTGGTTGAGAGAGTGTTTGGACAACAGAGTG
                  ************************************************************

HsCD00288876      GGTGTTAATGGCTTAGACAAAGCTGGAAGCACTGCCTTATACTGGGCTTGCCACGGGGGC
NM_012383.4       GGTGTTAATGGCTTAGACAAAGCTGGAAGCACTGCCTTATACTGGGCTTGCCACGGGGGC
                  ************************************************************

HsCD00288876      CACAAAGATATAGTGGAAATGCTATTTACTCAACCAAATATTGAACTGAACCAGCAGAAC
NM_012383.4       CACAAAGATATAGTGGAAATGCTATTTACTCAACCAAATATTGAACTGAACCAGCAGAAC
                  ************************************************************

HsCD00288876      AAGTTGGGAGATACAGCTTTGCATGCTGCTGCCTGGAAGGGTTATGCAGATATCGTCCAG
NM_012383.4       AAGTTGGGAGATACAGCTTTGCATGCTGCTGCCTGGAAGGGTTATGCAGATATCGTCCAG
                  ************************************************************

HsCD00288876      TTGTTTCTGGCAAAAGGTGCTAGAACAGACTTAAGAAACATTGAGAAGAAGCTGGCCTTC
NM_012383.4       TTGCTTCTGGCAAAAGGTGCTAGAACAGACTTAAGAAACATTGAGAAGAAGCTGGCCTTC
                  *** ********************************************************

HsCD00288876      GACATGGCTACCAATGCTGCCTGTGCATCTCTCCTGAAAAAGAAACAGGGAACAGATGCA
NM_012383.4       GACATGGCTACCAATGCTGCCTGTGCATCTCTCCTGAAAAAGAAACAGGGAACAGATGCA
                  ************************************************************

HsCD00288876      GTTCGAACATTAAGCAATGCCGAGGACTATCTCGATGATGAAGACTCAGATTAC
NM_012383.4       GTTCGAACATTAAGCAATGCCGAGGACTATCTCGATGATGAAGACTCAGATTAA
                  *****************************************************.