DNASU Plasmid | PDP2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PDP2

Gene Name: pyruvate dehyrogenase phosphatase catalytic subunit 2

Original Clone ID:

20770

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288919

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1590nts

Open reading frame: 1 to 1590

Reference Sequence Alignment

ATGTCAAGTACTGTGTCCTACTGGATCTTAAATTCTACAAGGAACAGCATTGCCACATTG
CAAGGGGGTAGACGCTTATACTCCAGGTATGTCTCAAATAGGAATAAATTAAAATGGAGG
CTCTTTTCCCGGGTGCCACCCACCCTAAACAGTTCCCCATGTGGTGGCTTTACTCTGTGC
AAAGCCTACAGACACACATCAACAGAGGAAGATGATTTTCACTTGCAACTCAGCCCTGAG
CAGATAAATGAAGTGCTTCGAGCTGGCGAGACAACCCACAAGATTCTTGACCTTGAAAGC
AGAGTCCCAAATTCAGTGTTGCGGTTTGAGAGCAACCAGCTGGCTGCCAATTCCCCAGTG
GAGGACCGGCGAGGTGTAGCCTCCTGCCTGCAAACCAATGGACTGATGTTTGGCATCTTC
GATGGACATGGTGGTCATGCATGTGCCCAAGCAGTGAGCGAGAGGCTCTTCTACTATGTG
GCAGTGTCCCTGATGTCCCACCAGACCCTGGAGCACATGGAGGGAGCTATGGAAAGCATG
AAACCCTTGCTGCCCATCCTGCATTGGCTCAAGCACCCAGGGGACAGTATCTACAAGGAT
GTCACATCTGTGCATCTTGACCACCTCCGTGTCTATTGGCAGGAACTGCTTGATTTGCAC
ATGGAAATGGGACTAAGCATTGAAGAAGCATTAATGTACTCCTTCCAGAGACTGGATTCT
GACATCTCGCTGGAAATCCAGGCCCCCCTGGAAGATGAGGTGACAAGGAACCTGTCACTC
CAGGTGGCTTTCTCTGGGGCAACAGCTTGCATGGCCCATGTTGATGGAATTCACTTGCAC
GTGGCAAATGCTGGTGACTGCCGAGCCATCCTTGGTGTCCAAGAGGACAATGGCATGTGG
TCTTGTCTGCCCCTTACACGTGACCACAATGCCTGGAACCAGGCCGAGCTGTCCCGGCTA
AAGAGGGAGCACCCTGAGTCAGAGGACAGGACGATCATCATGGAGGACAGGCTACTGGGC
GTCCTCATCCCCTGCAGGGCCTTTGGGGATGTTCAGCTGAAGTGGAGTAAAGAGTTGCAG
CGCAGCATTCTGGAGAGGGGCTTCAATACCGAGGCCCTCAACATTTACCAGTTCACACCC
CCACACTACTACACTCCACCCTACCTGACTGCTGAGCCTGAGGTCACATACCACAGGCTG
AGGCCCCAGGATAAGTTCCTTGTGCTGGCCTCAGATGGCCTGTGGGACATGCTGAGCAAT
GAGGACGTGGTAAGGCTGGTGGTGGGGCACCTGGCTGAGGCAGATTGGCACAAGACAGAC
CTGGCCCAGAGACCCGCCAACTTGGGGCTCATGCAGAGCCTGCTGCTGCAGAGGAAAGCC
AGCGGGCTCCACGAGGCTGACCAAAATGCAGCCACGCGGCTGATCAGACATGCCATCGGG
AACAATGAGTATGGGGAGATGGAGGCAGAGCGGCTGGCGGCGATGCTGACATTGCCAGAG
GACTTGGCGAGGATGTACAGGGATGATATCACTGTCACTGTGGTGTATTTTAACTCAGAA
TCAATCGGTGCATATTACAAGGGGGGTTAC

Protein sequence predicted based on nucleotide sequence

MSSTVSYWILNSTRNSIATLQGGRRLYSRYVSNRNKLKWRLFSRVPPTLNSSPCGGFTLC
KAYRHTSTEEDDFHLQLSPEQINEVLRAGETTHKILDLESRVPNSVLRFESNQLAANSPV
EDRRGVASCLQTNGLMFGIFDGHGGHACAQAVSERLFYYVAVSLMSHQTLEHMEGAMESM
KPLLPILHWLKHPGDSIYKDVTSVHLDHLRVYWQELLDLHMEMGLSIEEALMYSFQRLDS
DISLEIQAPLEDEVTRNLSLQVAFSGATACMAHVDGIHLHVANAGDCRAILGVQEDNGMW
SCLPLTRDHNAWNQAELSRLKREHPESEDRTIIMEDRLLGVLIPCRAFGDVQLKWSKELQ
RSILERGFNTEALNIYQFTPPHYYTPPYLTAEPEVTYHRLRPQDKFLVLASDGLWDMLSN
EDVVRLVVGHLAEADWHKTDLAQRPANLGLMQSLLLQRKASGLHEADQNAATRLIRHAIG
NNEYGEMEAERLAAMLTLPEDLARMYRDDITVTVVYFNSESIGAYYKGGY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 151

End on reference sequence 1740

Mutations:

None

Discrepancy:

t786g

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

PDP2

Symbol Nomenclature :

PDP2

SYNONYM :

PPM2C2

Designation :

PDPC 2|[Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 2, mitochondrial|protein phosphatase 2C, magnesium-dependent, catalytic subunit 2|pyruvate dehydrogenase phosphatase catalytic subunit 2|pyruvate dehydrogenase phosphatase isoenzyme 2|pyruvate dehydrogenase phosphatase, catalytic subunit 2

Full Nomenclature :

pyruvate dehyrogenase phosphatase catalytic subunit 2

GENEID :

57546

HGNC :

30263

MIM :

615499

Vega :

OTTHUMG00000137512

Target GenBank:

BC028030.1

Reference Sequence Alignment

  

HsCD00288919      ATGTCAAGTACTGTGTCCTACTGGATCTTAAATTCTACAAGGAACAGCATTGCCACATTG
NM_020786.2       ATGTCAAGTACTGTGTCCTACTGGATCTTAAATTCTACAAGGAACAGCATTGCCACATTG
                  ************************************************************

HsCD00288919      CAAGGGGGTAGACGCTTATACTCCAGGTATGTCTCAAATAGGAATAAATTAAAATGGAGG
NM_020786.2       CAAGGGGGTAGACGCTTATACTCCAGGTATGTCTCAAATAGGAATAAATTAAAATGGAGG
                  ************************************************************

HsCD00288919      CTCTTTTCCCGGGTGCCACCCACCCTAAACAGTTCCCCATGTGGTGGCTTTACTCTGTGC
NM_020786.2       CTCTTTTCCCGGGTGCCACCCACCCTAAACAGTTCCCCATGTGGTGGCTTTACTCTGTGC
                  ************************************************************

HsCD00288919      AAAGCCTACAGACACACATCAACAGAGGAAGATGATTTTCACTTGCAACTCAGCCCTGAG
NM_020786.2       AAAGCCTACAGACACACATCAACAGAGGAAGATGATTTTCACTTGCAACTCAGCCCTGAG
                  ************************************************************

HsCD00288919      CAGATAAATGAAGTGCTTCGAGCTGGCGAGACAACCCACAAGATTCTTGACCTTGAAAGC
NM_020786.2       CAGATAAATGAAGTGCTTCGAGCTGGCGAGACAACCCACAAGATTCTTGACCTTGAAAGC
                  ************************************************************

HsCD00288919      AGAGTCCCAAATTCAGTGTTGCGGTTTGAGAGCAACCAGCTGGCTGCCAATTCCCCAGTG
NM_020786.2       AGAGTCCCAAATTCAGTGTTGCGGTTTGAGAGCAACCAGCTGGCTGCCAATTCCCCAGTG
                  ************************************************************

HsCD00288919      GAGGACCGGCGAGGTGTAGCCTCCTGCCTGCAAACCAATGGACTGATGTTTGGCATCTTC
NM_020786.2       GAGGACCGGCGAGGTGTAGCCTCCTGCCTGCAAACCAATGGACTGATGTTTGGCATCTTC
                  ************************************************************

HsCD00288919      GATGGACATGGTGGTCATGCATGTGCCCAAGCAGTGAGCGAGAGGCTCTTCTACTATGTG
NM_020786.2       GATGGACATGGTGGTCATGCATGTGCCCAAGCAGTGAGCGAGAGGCTCTTCTACTATGTG
                  ************************************************************

HsCD00288919      GCAGTGTCCCTGATGTCCCACCAGACCCTGGAGCACATGGAGGGAGCTATGGAAAGCATG
NM_020786.2       GCAGTGTCCCTGATGTCCCACCAGACCCTGGAGCACATGGAGGGAGCTATGGAAAGCATG
                  ************************************************************

HsCD00288919      AAACCCTTGCTGCCCATCCTGCATTGGCTCAAGCACCCAGGGGACAGTATCTACAAGGAT
NM_020786.2       AAACCCTTGCTGCCCATCCTGCATTGGCTCAAGCACCCAGGGGACAGTATCTACAAGGAT
                  ************************************************************

HsCD00288919      GTCACATCTGTGCATCTTGACCACCTCCGTGTCTATTGGCAGGAACTGCTTGATTTGCAC
NM_020786.2       GTCACATCTGTGCATCTTGACCACCTCCGTGTCTATTGGCAGGAACTGCTTGATTTGCAC
                  ************************************************************

HsCD00288919      ATGGAAATGGGACTAAGCATTGAAGAAGCATTAATGTACTCCTTCCAGAGACTGGATTCT
NM_020786.2       ATGGAAATGGGACTAAGCATTGAAGAAGCATTAATGTACTCCTTCCAGAGACTGGATTCT
                  ************************************************************

HsCD00288919      GACATCTCGCTGGAAATCCAGGCCCCCCTGGAAGATGAGGTGACAAGGAACCTGTCACTC
NM_020786.2       GACATCTCGCTGGAAATCCAGGCCCCCCTGGAAGATGAGGTGACAAGGAACCTGTCACTC
                  ************************************************************

HsCD00288919      CAGGTGGCTTTCTCTGGGGCAACAGCTTGCATGGCCCATGTTGATGGAATTCACTTGCAC
NM_020786.2       CAGGTTGCTTTCTCTGGGGCAACAGCTTGCATGGCCCATGTTGATGGAATTCACTTGCAC
                  ***** ******************************************************

HsCD00288919      GTGGCAAATGCTGGTGACTGCCGAGCCATCCTTGGTGTCCAAGAGGACAATGGCATGTGG
NM_020786.2       GTGGCAAATGCTGGTGACTGCCGAGCCATCCTTGGTGTCCAAGAGGACAATGGCATGTGG
                  ************************************************************

HsCD00288919      TCTTGTCTGCCCCTTACACGTGACCACAATGCCTGGAACCAGGCCGAGCTGTCCCGGCTA
NM_020786.2       TCTTGTCTGCCCCTTACACGTGACCACAATGCCTGGAACCAGGCCGAGCTGTCCCGGCTA
                  ************************************************************

HsCD00288919      AAGAGGGAGCACCCTGAGTCAGAGGACAGGACGATCATCATGGAGGACAGGCTACTGGGC
NM_020786.2       AAGAGGGAGCACCCTGAGTCAGAGGACAGGACGATCATCATGGAGGACAGGCTACTGGGC
                  ************************************************************

HsCD00288919      GTCCTCATCCCCTGCAGGGCCTTTGGGGATGTTCAGCTGAAGTGGAGTAAAGAGTTGCAG
NM_020786.2       GTCCTCATCCCCTGCAGGGCCTTTGGGGATGTTCAGCTGAAGTGGAGTAAAGAGTTGCAG
                  ************************************************************

HsCD00288919      CGCAGCATTCTGGAGAGGGGCTTCAATACCGAGGCCCTCAACATTTACCAGTTCACACCC
NM_020786.2       CGCAGCATTCTGGAGAGGGGCTTCAATACCGAGGCCCTCAACATTTACCAGTTCACACCC
                  ************************************************************

HsCD00288919      CCACACTACTACACTCCACCCTACCTGACTGCTGAGCCTGAGGTCACATACCACAGGCTG
NM_020786.2       CCACACTACTACACTCCACCCTACCTGACTGCTGAGCCTGAGGTCACATACCACAGGCTG
                  ************************************************************

HsCD00288919      AGGCCCCAGGATAAGTTCCTTGTGCTGGCCTCAGATGGCCTGTGGGACATGCTGAGCAAT
NM_020786.2       AGGCCCCAGGATAAGTTCCTTGTGCTGGCCTCAGATGGCCTGTGGGACATGCTGAGCAAT
                  ************************************************************

HsCD00288919      GAGGACGTGGTAAGGCTGGTGGTGGGGCACCTGGCTGAGGCAGATTGGCACAAGACAGAC
NM_020786.2       GAGGACGTGGTAAGGCTGGTGGTGGGGCACCTGGCTGAGGCAGATTGGCACAAGACAGAC
                  ************************************************************

HsCD00288919      CTGGCCCAGAGACCCGCCAACTTGGGGCTCATGCAGAGCCTGCTGCTGCAGAGGAAAGCC
NM_020786.2       CTGGCCCAGAGACCCGCCAACTTGGGGCTCATGCAGAGCCTGCTGCTGCAGAGGAAAGCC
                  ************************************************************

HsCD00288919      AGCGGGCTCCACGAGGCTGACCAAAATGCAGCCACGCGGCTGATCAGACATGCCATCGGG
NM_020786.2       AGCGGGCTCCACGAGGCTGACCAAAATGCAGCCACGCGGCTGATCAGACATGCCATCGGG
                  ************************************************************

HsCD00288919      AACAATGAGTATGGGGAGATGGAGGCAGAGCGGCTGGCGGCGATGCTGACATTGCCAGAG
NM_020786.2       AACAATGAGTATGGGGAGATGGAGGCAGAGCGGCTGGCGGCGATGCTGACATTGCCAGAG
                  ************************************************************

HsCD00288919      GACTTGGCGAGGATGTACAGGGATGATATCACTGTCACTGTGGTGTATTTTAACTCAGAA
NM_020786.2       GACTTGGCGAGGATGTACAGGGATGATATCACTGTCACTGTGGTGTATTTTAACTCAGAA
                  ************************************************************

HsCD00288919      TCAATCGGTGCATATTACAAGGGGGGTTAC
NM_020786.2       TCAATCGGTGCATATTACAAGGGGGGTTAA
                  *****************************.