DNASU Plasmid | TNFRSF10D (H. sapiens ) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: TNFRSF10D

Gene Name: tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain

Original Clone ID:

42910

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00288948

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1161nts

Open reading frame: 1 to 1161

Reference Sequence Alignment

ATGGGACTTTGGGGACAAAGCGTCCCGACCGCCTCGAGCGCTCGAGCAGGGCGCTATCCA
GGAGCCAGGACAGCGTCGGGAACCAGACCATGGCTCCTGGACCCCAAGATCCTTAAGTTC
GTCGTCTTCATCGTCGCGGTTCTGCTGCCGGTCCGGGTTGACTCTGCCACCATCCCCCGG
CAGGACGAAGTTCCCCAGCAGACAGTGGCCCCACAGCAACAGAGGCGCAGCCTCAAGGAG
GAGGAGTGTCCAGCAGGATCTCATAGATCAGAATATACTGGAGCCTGTAACCCGTGCACA
GAGGGTGTGGATTACACCATTGCTTCCAACAATTTGCCTTCTTGCCTGCTATGTACAGTT
TGTAAATCAGGTCAAACAAATAAAAGTTCCTGTACCACGACCAGAGACACCGTGTGTCAG
TGTGAAAAAGGAAGCTTCCAGGATAAAAACTCCCCTGAGATGTGCCGGACGTGTAGAACA
GGGTGTCCCAGAGGGATGGTCAAGGTCAGTAATTGTACGCCCCGGAGTGACATCAAGTGC
AAAAATGAATCAGCTGCCAGTTCCACTGGGAAAACCCCAGCAGCGGAGGAGACAGTGACC
ACCATCCTGGGGATGCTTGCCTCTCCCTATCACTACCTTATCATCATAGTGGTTTTAGTC
ATCATTTTAGCTGTGGTTGTGGTTGGCTTTTCATGTCGGAAGAAATTCATTTCTTACCTC
AAAGGCATCTGCTCAGGTGGTGGAGGAGGTCCCGAACGTGTGCACAGAGTCCTTTTCCGG
CGGCGTTCATGTCCTTCACGAGTTCCTGGGGCGGAGGACAATGCCCGCAACGAGACCCTG
AGTAACAGATACTTGCAGCCCACCCAGGTCTCTGAGCAGGAAATCCAAGGTCAGGAGCTG
GCAGAGCTAACAGGTGTGACTGTAGAGTCGCCAGAGGAGCCACAGCGTCTGCTGGAACAG
GCAGAAGCTGAAGGGTGTCAGAGGAGGAGGCTGCTGGTTCCAGTGAATGACGCTGACTCC
GCTGACATCAGCACCTTGCTGGATGCCTCGGCAACACTGGAAGAAGGACATGCAAAGGAA
ACAATTCAGGACCAACTGGTGGGCTCCGAAAAGCTCTTTTATGAAGAAGATGAGGCAGGC
TCTGCTACGTCCTGCCTGTGC

Protein sequence predicted based on nucleotide sequence

MGLWGQSVPTASSARAGRYPGARTASGTRPWLLDPKILKFVVFIVAVLLPVRVDSATIPR
QDEVPQQTVAPQQQRRSLKEEECPAGSHRSEYTGACNPCTEGVDYTIASNNLPSCLLCTV
CKSGQTNKSSCTTTRDTVCQCEKGSFQDKNSPEMCRTCRTGCPRGMVKVSNCTPRSDIKC
KNESAASSTGKTPAAEETVTTILGMLASPYHYLIIIVVLVIILAVVVVGFSCRKKFISYL
KGICSGGGGGPERVHRVLFRRRSCPSRVPGAEDNARNETLSNRYLQPTQVSEQEIQGQEL
AELTGVTVESPEEPQRLLEQAEAEGCQRRRLLVPVNDADSADISTLLDASATLEEGHAKE
TIQDQLVGSEKLFYEEDEAGSATSCLC

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1244

Start on reference sequence 84

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

TNFRSF10D

Symbol Nomenclature :

TNFRSF10D

SYNONYM :

CD264; DCR2; TRAIL-R4; TRAILR4; TRUNDD

Designation :

Full Nomenclature :

tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain

GENEID :

8793

Locus Tag :

UNQ251/PRO288

HGNC :

11907

MIM :

603614

Vega :

OTTHUMG00000097845

Target GenBank:

BC052270.1

Reference Sequence Alignment

  

HsCD00288948      ATGGGACTTTGGGGACAAAGCGTCCCGACCGCCTCGAGCGCTCGAGCAGGGCGCTATCCA
NM_003840.4       ATGGGACTTTGGGGACAAAGCGTCCCGACCGCCTCGAGCGCTCGAGCAGGGCGCTATCCA
                  ************************************************************

HsCD00288948      GGAGCCAGGACAGCGTCGGGAACCAGACCATGGCTCCTGGACCCCAAGATCCTTAAGTTC
NM_003840.4       GGAGCCAGGACAGCGTCGGGAACCAGACCATGGCTCCTGGACCCCAAGATCCTTAAGTTC
                  ************************************************************

HsCD00288948      GTCGTCTTCATCGTCGCGGTTCTGCTGCCGGTCCGGGTTGACTCTGCCACCATCCCCCGG
NM_003840.4       GTCGTCTTCATCGTCGCGGTTCTGCTGCCGGTCCGGGTTGACTCTGCCACCATCCCCCGG
                  ************************************************************

HsCD00288948      CAGGACGAAGTTCCCCAGCAGACAGTGGCCCCACAGCAACAGAGGCGCAGCCTCAAGGAG
NM_003840.4       CAGGACGAAGTTCCCCAGCAGACAGTGGCCCCACAGCAACAGAGGCGCAGCCTCAAGGAG
                  ************************************************************

HsCD00288948      GAGGAGTGTCCAGCAGGATCTCATAGATCAGAATATACTGGAGCCTGTAACCCGTGCACA
NM_003840.4       GAGGAGTGTCCAGCAGGATCTCATAGATCAGAATATACTGGAGCCTGTAACCCGTGCACA
                  ************************************************************

HsCD00288948      GAGGGTGTGGATTACACCATTGCTTCCAACAATTTGCCTTCTTGCCTGCTATGTACAGTT
NM_003840.4       GAGGGTGTGGATTACACCATTGCTTCCAACAATTTGCCTTCTTGCCTGCTATGTACAGTT
                  ************************************************************

HsCD00288948      TGTAAATCAGGTCAAACAAATAAAAGTTCCTGTACCACGACCAGAGACACCGTGTGTCAG
NM_003840.4       TGTAAATCAGGTCAAACAAATAAAAGTTCCTGTACCACGACCAGAGACACCGTGTGTCAG
                  ************************************************************

HsCD00288948      TGTGAAAAAGGAAGCTTCCAGGATAAAAACTCCCCTGAGATGTGCCGGACGTGTAGAACA
NM_003840.4       TGTGAAAAAGGAAGCTTCCAGGATAAAAACTCCCCTGAGATGTGCCGGACGTGTAGAACA
                  ************************************************************

HsCD00288948      GGGTGTCCCAGAGGGATGGTCAAGGTCAGTAATTGTACGCCCCGGAGTGACATCAAGTGC
NM_003840.4       GGGTGTCCCAGAGGGATGGTCAAGGTCAGTAATTGTACGCCCCGGAGTGACATCAAGTGC
                  ************************************************************

HsCD00288948      AAAAATGAATCAGCTGCCAGTTCCACTGGGAAAACCCCAGCAGCGGAGGAGACAGTGACC
NM_003840.4       AAAAATGAATCAGCTGCCAGTTCCACTGGGAAAACCCCAGCAGCGGAGGAGACAGTGACC
                  ************************************************************

HsCD00288948      ACCATCCTGGGGATGCTTGCCTCTCCCTATCACTACCTTATCATCATAGTGGTTTTAGTC
NM_003840.4       ACCATCCTGGGGATGCTTGCCTCTCCCTATCACTACCTTATCATCATAGTGGTTTTAGTC
                  ************************************************************

HsCD00288948      ATCATTTTAGCTGTGGTTGTGGTTGGCTTTTCATGTCGGAAGAAATTCATTTCTTACCTC
NM_003840.4       ATCATTTTAGCTGTGGTTGTGGTTGGCTTTTCATGTCGGAAGAAATTCATTTCTTACCTC
                  ************************************************************

HsCD00288948      AAAGGCATCTGCTCAGGTGGTGGAGGAGGTCCCGAACGTGTGCACAGAGTCCTTTTCCGG
NM_003840.4       AAAGGCATCTGCTCAGGTGGTGGAGGAGGTCCCGAACGTGTGCACAGAGTCCTTTTCCGG
                  ************************************************************

HsCD00288948      CGGCGTTCATGTCCTTCACGAGTTCCTGGGGCGGAGGACAATGCCCGCAACGAGACCCTG
NM_003840.4       CGGCGTTCATGTCCTTCACGAGTTCCTGGGGCGGAGGACAATGCCCGCAACGAGACCCTG
                  ************************************************************

HsCD00288948      AGTAACAGATACTTGCAGCCCACCCAGGTCTCTGAGCAGGAAATCCAAGGTCAGGAGCTG
NM_003840.4       AGTAACAGATACTTGCAGCCCACCCAGGTCTCTGAGCAGGAAATCCAAGGTCAGGAGCTG
                  ************************************************************

HsCD00288948      GCAGAGCTAACAGGTGTGACTGTAGAGTCGCCAGAGGAGCCACAGCGTCTGCTGGAACAG
NM_003840.4       GCAGAGCTAACAGGTGTGACTGTAGAGTTGCCAGAGGAGCCACAGCGTCTGCTGGAACAG
                  **************************** *******************************

HsCD00288948      GCAGAAGCTGAAGGGTGTCAGAGGAGGAGGCTGCTGGTTCCAGTGAATGACGCTGACTCC
NM_003840.4       GCAGAAGCTGAAGGGTGTCAGAGGAGGAGGCTGCTGGTTCCAGTGAATGACGCTGACTCC
                  ************************************************************

HsCD00288948      GCTGACATCAGCACCTTGCTGGATGCCTCGGCAACACTGGAAGAAGGACATGCAAAGGAA
NM_003840.4       GCTGACATCAGCACCTTGCTGGATGCCTCGGCAACACTGGAAGAAGGACATGCAAAGGAA
                  ************************************************************

HsCD00288948      ACAATTCAGGACCAACTGGTGGGCTCCGAAAAGCTCTTTTATGAAGAAGATGAGGCAGGC
NM_003840.4       ACAATTCAGGACCAACTGGTGGGCTCCGAAAAGCTCTTTTATGAAGAAGATGAGGCAGGC
                  ************************************************************

HsCD00288948      TCTGCTACGTCCTGCCTGTGC
NM_003840.4       TCTGCTACGTCCTGCCTGTGA
                  ********************.