CASP14 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  CASP14
Gene Name:  caspase 14, apoptosis-related cysteine peptidase
Original Clone ID:
43597
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00289006
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 729nts
Open reading frame: 1 to 729

ATGAGCAATCCGCGGTCTTTGGAAGAGGAGAAATATGATATGTCAGGTGCCCGCCTGGCC
CTAATACTGTGTGTCACCAAAGCCCGGGAAGGTTCCGAAGAAGACCTGGATGCTCTGGAA
CACATGTTTCGGCAGCTGAGATTCGAAAGCACCATGAAAAGAGACCCCACTGCCGAGCAA
TTCCAGGAAGAGCTGGAAAAATTCCAGCAGGCCATCGATTCCCGGGAAGATCCCGTCAGT
TGTGCCTTCGTGGTACTCATGGCTCACGGGAGGGAAGGCTTCCTCAAGGGAGAAGATGGG
GAGATGGTCAAGCTGGAGAATCTCTTCGAGGCCCTGAACAACAAGAACTGCCAGGCCCTG
CGAGCTAAGCCCAAGGTGTACATCATACAGGCCTGTCGAGGAGAACAAAGGGACCCCGGT
GAAACAGTAGGTGGAGATGAGATTGTGATGGTCATCAAAGACAGCCCACAAACCATCCCA
ACATACACAGATGCCTTGCACGTTTATTCCACGGTAGAGGGATACATCGCCTACCGACAT
GATCAGAAAGGCTCATGCTTTATCCAGACCCTGGTGGATGTGTTCACGAAGAGGAAAGGA
CATATCTTGGAACTTCTGACAGAGGTGACCCGGCGGATGGCAGAAGCAGAGCTGGTTCAA
GAAGGAAAAGCAAGGAAAACGAACCCTGAAATCCAAAGCACCCTCCGGAAACGGCTGTAT
CTGCAGTAC

Protein sequence predicted based on nucleotide sequence

MSNPRSLEEEKYDMSGARLALILCVTKAREGSEEDLDALEHMFRQLRFESTMKRDPTAEQ
FQEELEKFQQAIDSREDPVSCAFVVLMAHGREGFLKGEDGEMVKLENLFEALNNKNCQAL
RAKPKVYIIQACRGEQRDPGETVGGDEIVMVIKDSPQTIPTYTDALHVYSTVEGYIAYRH
DQKGSCFIQTLVDVFTKRKGHILELLTEVTRRMAEAELVQEGKARKTNPEIQSTLRKRLY
LQY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 49
End on reference sequence 777
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable