DNASU Plasmid | VAMP2 (Homo sapiens) in pENTR223 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: VAMP2

Gene Name: vesicle-associated membrane protein 2 (synaptobrevin 2)

Original Clone ID:

9622

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00289279

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 351nts

Open reading frame: 1 to 351

Reference Sequence Alignment

ATGTCTGCTACCGCTGCCACGGCCCCCCCTGCTGCCCCGGCTGGGGAGGGTGGTCCCCCT
GCACCCCCTCCAAACCTCACCAGTAACAGGAGACTGCAGCAGACCCAGGCCCAGGTGGAT
GAGGTGGTGGACATCATGAGGGTGAACTTGGACAAGGTCCTGGAGCGAGACCAGAAGCTG
TCGGAGCTGGACGACCGTGCAGATGCACTCCAGGCGGGGGCCTCCCAGTTTGAAACAAGC
GCAGCCAAGCTCAAGCGCAAATACTGGTGGAAAAACCTCAAGATGATGATCATCTTGGGA
GTGATTTGCGCCATCATCCTCATCATCATCATAGTTTACTTCAGCACTTAC

Protein sequence predicted based on nucleotide sequence

MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNLDKVLERDQKL
SELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFSTY

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 429

Start on reference sequence 79

Mutations:

None

Discrepancy:

g148t,V50L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223

Synonyms:

pDONR223, pENTR223-XnCDSYn

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php; also see supplemental figure 1 of the paper PubMed ID 15489335.

Size (bp):

2815

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Mark Vidal

Publications:

PMID: 15489335

Title: Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTGCCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTACCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ori

bacterial origin of replication

2220

2753

gene fragment

pas

5p stem of pBR primose assembly site

gene fragment

pas prime

3p stem of pBR pas(L)

105

116

recombination site

attL1

attL1 recombination site

570

665

recombination site

attL2

attL2 recombination site

793

696

selectable marker

SpnR

spectinomycin resistance gene

1062

2072

trxn termination sequence

T1-T2

rrnBT1 and T2 terminators

510

224

Gene

Gene Symbol :

VAMP2

Symbol Nomenclature :

VAMP2

SYNONYM :

SYB2; VAMP-2

Designation :

synaptobrevin 2|synaptobrevin-2|vesicle associated membrane protein 2|vesicle-associated membrane protein 2

Full Nomenclature :

vesicle-associated membrane protein 2 (synaptobrevin 2)

GENEID :

6844

HGNC :

12643

MIM :

185881

Vega :

OTTHUMG00000150254

Target GenBank:

BC019608.1

Reference Sequence Alignment

  

HsCD00289279      ATGTCTGCTACCGCTGCCACGGCCCCCCCTGCTGCCCCGGCTGGGGAGGGTGGTCCCCCT
NM_014232.2       ATGTCTGCTACCGCTGCCACGGCCCCCCCTGCTGCCCCGGCTGGGGAGGGTGGTCCCCCT
                  ************************************************************

HsCD00289279      GCACCCCCTCCAAACCTCACCAGTAACAGGAGACTGCAGCAGACCCAGGCCCAGGTGGAT
NM_014232.2       GCACCCCCTCCAAACCTCACCAGTAACAGGAGACTGCAGCAGACCCAGGCCCAGGTGGAT
                  ************************************************************

HsCD00289279      GAGGTGGTGGACATCATGAGGGTGAACTTGGACAAGGTCCTGGAGCGAGACCAGAAGCTG
NM_014232.2       GAGGTGGTGGACATCATGAGGGTGAACGTGGACAAGGTCCTGGAGCGAGACCAGAAGCTG
                  *************************** ********************************

HsCD00289279      TCGGAGCTGGACGACCGTGCAGATGCACTCCAGGCGGGGGCCTCCCAGTTTGAAACAAGC
NM_014232.2       TCGGAGCTGGACGACCGTGCAGATGCACTCCAGGCGGGGGCCTCCCAGTTTGAAACAAGC
                  ************************************************************

HsCD00289279      GCAGCCAAGCTCAAGCGCAAATACTGGTGGAAAAACCTCAAGATGATGATCATCTTGGGA
NM_014232.2       GCAGCCAAGCTCAAGCGCAAATACTGGTGGAAAAACCTCAAGATGATGATCATCTTGGGA
                  ************************************************************

HsCD00289279      GTGATTTGCGCCATCATCCTCATCATCATCATAGTTTACTTCAGCACTTAC
NM_014232.2       GTGATTTGCGCCATCATCCTCATCATCATCATAGTTTACTTCAGCACTTAA
                  **************************************************.

Pathways

NCI :

Effects of Botulinum toxin

NCI :

Insulin-mediated glucose transport

Panther :

5HT1 type receptor mediated signaling pathway

Panther :

5HT2 type receptor mediated signaling pathway

Panther :

5HT3 type receptor mediated signaling pathway

Panther :

5HT4 type receptor mediated signaling pathway

Panther :

Adrenaline and noradrenaline biosynthesis

Panther :

Alpha adrenergic receptor signaling pathway

Panther :

Beta1 adrenergic receptor signaling pathway

Panther :

Beta2 adrenergic receptor signaling pathway

Panther :

Beta3 adrenergic receptor signaling pathway

Panther :

Cortocotropin releasing factor receptor signaling pathway

Panther :

Dopamine receptor mediated signaling pathway

Panther :

Ionotropic glutamate receptor pathway

Panther :

Metabotropic glutamate receptor group II pathway

Panther :

Metabotropic glutamate receptor group III pathway

Panther :

Muscarinic acetylcholine receptor 1 and 3 signaling pathway

Panther :

Muscarinic acetylcholine receptor 2 and 4 signaling pathway

Panther :

Nicotinic acetylcholine receptor signaling pathway

Panther :

Opioid prodynorphin pathway

Panther :

Opioid proenkephalin pathway

Panther :

Opioid proopiomelanocortin pathway

Panther :

Oxytocin receptor mediated signaling pathway

Panther :

Thyrotropin-releasing hormone receptor signaling pathway

Reactome :

Acetylcholine Neurotransmitter Release Cycle

Reactome :

Clathrin derived vesicle budding

Reactome :

Disease

Reactome :

Dopamine Neurotransmitter Release Cycle

Reactome :

GABA synthesis, release, reuptake and degradation

Reactome :

Glutamate Neurotransmitter Release Cycle

Reactome :

Golgi Associated Vesicle Biogenesis

Reactome :

Insulin processing

Reactome :

Integration of energy metabolism

Reactome :

Lysosome Vesicle Biogenesis

Reactome :

Membrane Trafficking

Reactome :

Metabolism

Reactome :

Metabolism of proteins

Reactome :

Neuronal System

Reactome :

Neurotoxicity of clostridium toxins

Reactome :

Neurotransmitter Release Cycle

Reactome :

Norepinephrine Neurotransmitter Release Cycle

Reactome :

Peptide hormone metabolism

Reactome :

Regulation of insulin secretion

Reactome :

Serotonin Neurotransmitter Release Cycle

Reactome :

Toxicity of botulinum toxin type B (BoNT/B)

Reactome :

Toxicity of botulinum toxin type D (BoNT/D)

Reactome :

Toxicity of botulinum toxin type F (BoNT/F)

Reactome :

Toxicity of botulinum toxin type G (BoNT/G)

Reactome :

trans-Golgi Network Vesicle Budding

Reactome :

Translocation of GLUT4 to the plasma membrane

Reactome :

Transmission across Chemical Synapses

WikiPathway :

Insulin Signaling

Protein

UniProt :

P63027

HPRD :

01717

Clone

Cloning Information :

472516

HIP Master Clone ID :

395434

Original Clone ID :

9622

Organism

Species Specific ID:

6844

Genome

Chromosome :

Map Location :

17p13.1

Ensembl :

ENSG00000220205

Reagents

Labome :

VAMP2-antibody