Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
35365
Keyword:
None
Species:
Methanococcus maripaludis S2
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Joint Center for Structural Genomics
MmCD00291248
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 822nts
Open reading frame: 51 to 872

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGTTGAAAT
TTGGAACCGCTGGAATACCAATAAATGCTAAAAATACAGAAAGCGCACTCGAATATTTAA
AAGAAATTAATCTTGATGCGATGGAGCTTGAATTCGTACGTGGCGTAAATTTAAAAGCTG
AAAAAGCAGAAACTTTGGCAAAAAATTTGAAAATTACACTCAGTGCTCATGCCCCTTATT
ATATCAATTTAAATGCAAAAGAACCTGAAAAAATCGAATCCAGCATTAAAAGAATCGTTG
ATTCTGCAAAAATAATTAGTATATTCAATAAAAAAAATTCTGAAAATAAAAACGTGGTTT
TTCATTCTGGATTTTATTTAAAACAGGATAAAGATTTAGTTTATGGGAAAATAAAAGCAA
ATATTCAAAAAATACTTGAAATAATGAATGAAAATAAAATAAATGCGATTTTAAGGCCCG
AAACTACTGGAAAAATCTCACAATTTGGAAGCGTTGATGAATTAATCCAATTGTCAGCTG
AAATTGACATTATGCCGTGCATTGACTTTTCGCACGTATTCGCACGAAGTCTTGGAAAAA
TAAATGATTATGATTCATTTTCAAATATATTATCAAAACTTGAAAATAATCTTGGAAAAA
AAGGAATTAAGGATATGCACATCCACATTTCAGGAATTGATTTTGGAAACGGGGGCGAAA
AAAAGCATTTTCCAATTTTAAATTCTGAATTTAATTATATGGCAGTTTTAAAAGCTTTAA
AAGATTTTGAATGTTCTGGAACGGTTATTTGTGAAAGTCCTCGATTAGAATACGACGCAG
TTATTTTAAAAAAGAATTATGAAGAGTTATAACGCGACTTAATTAA

Protein sequence predicted based on nucleotide sequence

MLKFGTAGIPINAKNTESALEYLKEINLDAMELEFVRGVNLKAEKAETLAKNLKITLSAH
APYYINLNAKEPEKIESSIKRIVDSAKIISIFNKKNSENKNVVFHSGFYLKQDKDLVYGK
IKANIQKILEIMNENKINAILRPETTGKISQFGSVDELIQLSAEIDIMPCIDFSHVFARS
LGKINDYDSFSNILSKLENNLGKKGIKDMHIHISGIDFGNGGEKKHFPILNSEFNYMAVL
KALKDFECSGTVICESPRLEYDAVILKKNYEEL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 822
Mutations:
None
Discrepancy:
g30a,P10P;g81t,K27N;
a90g,A30A;t108a,V36V;
t114c,G38G;t142a,S48T;
a147g,L49L;c156t,N52N;
c177t,A59A;t183c,A61A;
c186t,P62P;g210a,K70K;
a231c,S77S;t234c,S78S;
c264a,I88I;c267t,I89I;
t276c,F92F;g333a,K111K;
a356g,E119G;t368c,V123A;
a387t,L129L;a388g,K130E;
g397a,D133N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Protein_Purified
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in 379582
Recommended expression in:
Not Applicable