Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
11137c1BCt8p1
Keyword:
None
Species:
Bacillus subtilis subsp. subtilis str. 168
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"C-terminal 6-His, TOPO"
Mutation / Discrepancy:
No / No
Publications:
PMID: 23416295
Title: Alpha-Galacturonidase(s): A new class of Family 4 glycoside hydrolases with strict specificity and a unique CHEV active site motif
Title: Alpha-Galacturonidase(s): A new class of Family 4 glycoside hydrolases with strict specificity and a unique CHEV active site motif
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
BsCD00292418
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1344nts
Open reading frame: 51 to 1394
TTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTGATCAGATTA
AGATTGCCTATATCGGCGGGGGGTCCCAAGGCTGGGCCAGAAGCCTGATGAGTGATTTGT
CGATTGATGAACGGATGTCAGGCACGGTGGCGCTCTATGATCTTGATTTTGAAGCCGCTC
AGAAAAATGAAGTGATCGGCAATCACAGCGGGAACGGCAGATGGAGATATGAGGCTGTTT
CTACTTTGAAAAAGGCGTTATCAGCCGCGGATATCGTCATTATTTCCATTTTGCCGGGAT
CACTGGATGATATGGAAGTCGATGTGCACTTGCCTGAGCGCTGCGGTATTTATCAGTCTG
TAGGTGATACTGTCGGGCCTGGAGGGATCATCAGGGGCTTGCGTGCTGTGCCGATATTTG
CGGAAATTGCCCGGGCAATAAGAGACTACGCACCTGAATCATGGGTCATTAATTATACAA
ACCCGATGTCTGTCTGTACAAGAGTGCTGTATAAAGTGTTTCCCGGCATCAAAGCGATTG
GCTGCTGCCACGAGGTATTCGGGACGCAAAAGCTATTGGCGGAAATGGTCACGGAAAGGC
TGGGGATTGAGGTGCCGCGGCGTGAGGATATCCGCGTTAATGTGCTCGGCATTAACCATT
TTACATGGATCACAAAAGCCTCTTACCGCCATATTGACCTGTTGCCTATATTCCGTGAAT
TCAGCGCGCACTATGGGGAATCAGGATATGAGCTGGAGGGGGAATGCTGGAGGGACAGCG
TCTTTTGTTCGGCACACCGTGTTGCGTTTGATTTATTTGAAACGTATGGCGCCATCCCTG
CTGCGGGTGACAGGCATCTGGCAGAGTTTCTTCCCGGCCCATACCTCAAACAGCCTGAAG
TATGGAAATTTCATCTCACACCTATATCATTCAGAAAACAAGACAGAGCTGAGAAACGAC
AAGAAACAGAACGGTTGATCGTGCAACAACGGGGCGTTGCTGAGAAAGCTTCGGGAGAAG
AAGGCGTGAACATCATAGCGGCTCTTCTCGGATTGGGTGAACTCGTCACAAATGTAAATA
TGCCGAATCAAGGCCAGGTCTTAAACCTTCCTATACAAGCCATTGTCGAAACAAACGCAT
TCATCACAAGGAACCGTGTCCAGCCGATTTTGTCCGGAGCGCTTCCGAAAGGTGTGGAAA
TGCTGGCGGCTAGGCATATATCCAATCAGGAGGCAGTGGCGGACGCAGGTTTAACAAAGG
ATACTGGCCTCGCGTTTCAAGCCTTCCTCAATGACCCGCTTGTCCAGATTGACCGCAGTG
ATGCAGAGCAGCTTTTCAATGACATGCTCCAATGCATCATGCAAAGCGAAGGGCATCATC
ACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGACGCACTCGAGCAC
CAC
Protein sequence predicted based on nucleotide sequence
DQIKIAYIGGGSQGWARSLMSDLSIDERMSGTVALYDLDFEAAQKNEVIGNHSGNGRWRY EAVSTLKKALSAADIVIISILPGSLDDMEVDVHLPERCGIYQSVGDTVGPGGIIRGLRAV PIFAEIARAIRDYAPESWVINYTNPMSVCTRVLYKVFPGIKAIGCCHEVFGTQKLLAEMV TERLGIEVPRREDIRVNVLGINHFTWITKASYRHIDLLPIFREFSAHYGESGYELEGECW RDSVFCSAHRVAFDLFETYGAIPAAGDRHLAEFLPGPYLKQPEVWKFHLTPISFRKQDRA EKRQETERLIVQQRGVAEKASGEEGVNIIAALLGLGELVTNVNMPNQGQVLNLPIQAIVE TNAFITRNRVQPILSGALPKGVEMLAARHISNQEAVADAGLTKDTGLAFQAFLNDPLVQI DRSDAEQLFNDMLQCIMQSEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1344
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11137c
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
