Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
13502c2BCt15p1
Keyword:
None
Species:
Shigella flexneri 2a str. 2457T
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"C-terminal 6-His, TOPO"
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
SfCD00292525
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1665nts
Open reading frame: 51 to 1715

TCCCTCTGAATATTTTGTTAACTTTAGAAGGAGATATACATATGTCCCTTCTTCCTGTAA
ATAATCCCCCCCTATCCACTGGAAACGTCTCTTTTTACAGAACTACATCAATCGACAATG
TTCACAATAATTATCTCTCCGAATGGGTTGAATGGACTAAAAACAGCATTTCCGGAGAAA
ACAGGGAAACTGCTTTTACCCGGCTCCAATTATGTCTGGAGAACAGTGAAACATCGTTGG
ACTTATCTTGTTTAGGTCTCAGATCTCTACCACGATTGCCTGACAATCTTGATGAAATTA
ATGTAAGCAATAACCAACTATCAATGCTCCCCGAGCTACCAAGGGCATTGAAAGAGCTGA
ATGCAAGCAGTAATCAATTATCTGCACTTCCTGAATTACCAGTGTCGCTGGAATATATAA
ATGTGAGTGATAACCATTTGTTCGCACTTCCTGAATTACCTGCGTCACTAGAATATATTA
ATGTAAGTGACAATCACCTGTCTGTACTTCCGAGGTTACCAATGTCATTGGAATTACTTG
ATGCAGCCAGAAATGCTTTGGAAGTAATACCAGATTTTCCAGAAAGAGATGATCATATTA
TAAGAATATTCTGGCTTAATCAGAACCGGATCACGGCAATTCCGGAAAGCATACTTGGCC
TCAGTTCTGATAGCGTTGTCAATCTTAGAGAAAATCAACTATCTCCCAGAATAATGCAAA
CTTTGTTACAACAAACCGCCCAACCGGACTACCACGGCCCACGGATTTACTTCTCCATGA
GTGACGGACAACAGAATACACTCCATCGCCCCCTGGCTGATGCCGTGACAGCATGGTTCC
CGGAAAACAAACAATCTGATGTATCACAGATATGGCATGCTTTTGAACATGAAGAGCACG
CCAACACCTTTTCCGCGTTCCTTGACCGCCTTTCCGATACCGTCTCTGCACGCAATACCT
CCGGATTCCGTGAACAGGTCGCTGCATGGCTGGAAAAACTCAGTGCCTCTGCGGAGCTTC
GACAGCAGTCTTTCGCTGTTGCTGCTGATGCCACTGAGAGCTGTGAGGACCGTGTCGCGC
TCACATGGAACAATCTCCGGAAAACCCTCCTGGTCCATCAGGCATCAGAAGGCCTTTTCG
ATAATGATACCGGCGCTCTGCTCTCCCTGGGCAGGGAAATGTTCCGCCTCGAAATTCTGG
AGGATATTGCCCGGGATAAAGTCAGAACTCTCCATTTTGTGGATGAGATAGAAGTCTACC
TGGCCTTCCAGACCATGCTCGCAGAGAAACTTCAGCTCTCCACTGCCGTGAAGGAAATGC
GTTTCTATGGCGTGTCGGGAGTGACAGCAAATGACCTCCGCACTGCCGAAGCCATGGTCA
GAAGCCGTGAAGAGAATGAATTTACGGACTGGTTCTCCCTCTGGGGACCATGGCATGCTG
TACTGAAGCGTACGGAAGCTGACCGCTGGGCGCAGGCAGAAGAGCAGAAATATGAGATGC
TGGAGAATGAGTACCCTCAGAGGGTGGCTGACCGGCTGAAAGCATCAGGTCTGAGCGGTG
ATGCGGATGCGGAGAGGGAAGCCGGTGCACAGGTGATGCGTGAGACTGAACAGCAGATTT
ACCGTCAGCTGACTGACGAGGTACTGGCCCTGCGATTGCCTGAAAACGGCTCACAACTGC
ACCATTCAGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAG
CTTGCGGCCGCACTCGAGCACCAC

Protein sequence predicted based on nucleotide sequence

LPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGENRETAFTRLQLCLENSE
TSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALKELNASSNQLSALPELPVSL
EYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMSLELLDAARNALEVIPDFPERD
DHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQLSPRIMQTLLQQTAQPDYHGPRIY
FSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSA
RNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQASE
GLFDNDTGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAV
KEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEEQK
YEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLPENG
SQLHHSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1665
Mutations:
None
Discrepancy:
t1609c,S537P;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-13502c
Recommended expression in:
Not Applicable