DNASU Plasmid | ADRA2A (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: ADRA2A

Gene Name: adrenoceptor alpha 2A

Original Clone ID:

ATCG3D_13

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

GPCR Network

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA 2.0 for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network

HsCD00292909

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1452nts

Open reading frame: 7 to 1458

Reference Sequence Alignment

GGATCCATGAAGACCATCATCGCACTGAGCTATATTTTCTGCCTGGTTTTCGCGGACTAT
AAAGACGATGATGACATGGGTTCTCTGCAACCGGATGCAGGTAATGCGAGCTGGAACGGT
ACCGAAGCGCCTGGCGGCGGTGCGCGTGCCACGCCGTACAGCCTGCAGGTCACTCTGACT
CTGGTCTGCCTGGCCGGTCTGCTGATGCTGCTGACCGTTTTCGGCAACGTTCTGGTCATT
ATCGCGGTATTTACTTCTCGTGCGCTGAAAGCACCACAAAACCTGTTTCTGGTGTCCCTG
GCATCTGCAGATATCCTGGTGGCTACTCTGGTGATCCCTTTTTCTCTGGCCAATGAAGTG
ATGGGCTATTGGTACTTCGGTAAAGCATGGTGCGAAATCTACCTGGCTCTGGACGTGCTG
TTTTGCACTTCCTCTATCGTGCACCTGTGTGCCATCTCTCTGGATCGTTACTGGTCTATC
ACCCAGGCCATCGAATATAACCTGAAGCGCACGCCGCGCCGTATTAAAGCAATTATCATT
ACCGTCTGGGTCATCTCCGCTGTTATCTCTTTCCCTCCGCTGATTTCCATCGAGAAGAAG
GGTGGCGGTGGTGGTCCACAGCCTGCTGAACCGCGTTGCGAAATCAATGATCAGAAGTGG
TACGTTATTAGCAGCTGTATTGGTTCTTTCTTTGCCCCATGCCTGATTATGATCCTGGTG
TACGTGCGTATCTATCAGATCGCCAAACGCCGTACCCGCGTTCCGCCGTCCCGCCGTGGC
CCGGACGCTGTGGCGGCTCCGCCTGGTGGTACCGAGCGTCGTCCGAACGGCCTGGGCCCA
GAACGTAGCGCAGGCCCGGGTGGCGCGGAAGCCGAACCGCTGCCGACCCAGCTGAACGGT
GCGCCTGGTGAACCAGCCCCGGCTGGTCCACGCGATACTGACGCGCTGGATCTGGAGGAG
TCCTCTTCCTCTGACCACGCGGAGCGTCCGCCTGGCCCGCGTCGTCCAGAGCGTGGTCCA
CGTGGTAAAGGCAAAGCCCGCGCTTCCCAGGTTAAGCCGGGTGATAGCCTGCCTCGCCGT
GGCCCTGGTGCCACTGGTATTGGCACGCCAGCGGCGGGTCCGGGCGAAGAACGTGTAGGC
GCAGCGAAGGCATCCCGTTGGCGTGGTCGCCAGAACCGTGAGAAGCGTTTCACGTTCGTC
CTGGCGGTGGTAATTGGTGTATTTGTAGTGTGTTGGTTCCCGTTCTTTTTCACCTACACC
CTGACTGCCGTGGGTTGCTCCGTGCCACGCACTCTGTTCAAATTCTTTTTCTGGTTCGGC
TACTGTAACTCTTCCCTGAACCCGGTTATCTATACTATCTTCAACCACGATTTTCGTCGT
GCGTTCAAAAAGATCCTGTGTCGCGGTGATCGTAAACGCATCGTGCACCACCATCACCAT
CACCATCACCATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMGSLQPDAGNASWNGTEAPGGGARATPYSLQVTLTLV
CLAGLLMLLTVFGNVLVIIAVFTSRALKAPQNLFLVSLASADILVATLVIPFSLANEVMG
YWYFGKAWCEIYLALDVLFCTSSIVHLCAISLDRYWSITQAIEYNLKRTPRRIKAIIITV
WVISAVISFPPLISIEKKGGGGGPQPAEPRCEINDQKWYVISSCIGSFFAPCLIMILVYV
RIYQIAKRRTRVPPSRRGPDAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAP
GEPAPAGPRDTDALDLEESSSSDHAERPPGPRRPERGPRGKGKARASQVKPGDSLPRRGP
GATGIGTPAAGPGEERVGAAKASRWRGRQNREKRFTFVLAVVIGVFVVCWFPFFFTYTLT
AVGCSVPRTLFKFFFWFGYCNSSLNPVIYTIFNHDFRRAFKKILCRGDRKRIVHHHHHHH
HHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: kanamycin

Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_1

Find experimental protocols in GPCR-10

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

ADRA2A

Symbol Nomenclature :

ADRA2A

SYNONYM :

ADRA2; ADRA2R; ADRAR; ALPHA2AAR; ZNF32

Designation :

Full Nomenclature :

adrenoceptor alpha 2A

GENEID :

150

HGNC :

281

MIM :

104210

Vega :

OTTHUMG00000019050

Target GenBank:

NP_000672

Reference Sequence Alignment

  

HsCD00292909      GGATCCATGAAGACCATCATCGCACTGAGCTATATTTTCTGCCTGGTTTTCGCGGACTAT
NM_000681.3       ------------------------------ATGTTCCGCCAGGAGCAGCCGTTGGCCGAG
                                                :: :*   * .  :* :      **.* * 

HsCD00292909      AAAGACGATGATGACATGGGTTCTCTGCAACCGGATGCAGGTAATGCGAGCTGGAACGGT
NM_000681.3       GGCAGCTTTGCGCCCATGGGCTCCCTGCAGCCGGACGCGGGCAACGCGAGCTGGAACGGG
                  .....* :**.  .****** ** *****.***** **.** ** ************** 

HsCD00292909      ACCGAAGCGCCTGGCGGCGGTGCGCGTGCCACGCCGTACAGCCTGCAGGTCACTCTGACT
NM_000681.3       ACCGAGGCGCCGGGGGGCGGCGCCCGGGCCACCCCTTACTCCCTGCAGGTGACGCTGACG
                  *****.***** ** ***** ** ** ***** ** ***: ********* ** ***** 

HsCD00292909      CTGGTCTGCCTGGCCGGTCTGCTGATGCTGCTGACCGTTTTCGGCAACGTTCTGGTCATT
NM_000681.3       CTGGTGTGCCTGGCCGGCCTGCTCATGCTGCTCACCGTGTTCGGCAACGTGCTCGTCATC
                  ***** *********** ***** ******** ***** *********** ** ***** 

HsCD00292909      ATCGCGGTATTTACTTCTCGTGCGCTGAAAGCACCACAAAACCTGTTTCTGGTGTCCCTG
NM_000681.3       ATCGCCGTGTTCACGAGCCGCGCGCTCAAGGCGCCCCAAAACCTCTTCCTGGTGTCTCTG
                  ***** **.** ** :  ** ***** **.**.**.******** ** ******** ***

HsCD00292909      GCATCTGCAGATATCCTGGTGGCTACTCTGGTGATCCCTTTTTCTCTGGCCAATGAAGTG
NM_000681.3       GCCTCGGCCGACATCCTGGTGGCCACGCTCGTCATCCCTTTCTCGCTGGCCAACGAGGTC
                  **.** **.** *********** ** ** ** ******** ** ******** **.** 

HsCD00292909      ATGGGCTATTGGTACTTCGGTAAAGCATGGTGCGAAATCTACCTGGCTCTGGACGTGCTG
NM_000681.3       ATGGGCTACTGGTACTTCGGCAAGGCTTGGTGCGAGATCTACCTGGCGCTCGACGTGCTC
                  ******** *********** **.**:********.*********** ** ******** 

HsCD00292909      TTTTGCACTTCCTCTATCGTGCACCTGTGTGCCATCTCTCTGGATCGTTACTGGTCTATC
NM_000681.3       TTCTGCACGTCGTCCATCGTGCACCTGTGCGCCATCAGCCTGGACCGCTACTGGTCCATC
                  ** ***** ** ** ************** ******:  ***** ** ******** ***

HsCD00292909      ACCCAGGCCATCGAATATAACCTGAAGCGCACGCCGCGCCGTATTAAAGCAATTATCATT
NM_000681.3       ACACAGGCCATCGAGTACAACCTGAAGCGCACGCCGCGCCGCATCAAGGCCATCATCATC
                  **.***********.** *********************** ** **.**.** ***** 

HsCD00292909      ACCGTCTGGGTCATCTCCGCTGTTATCTCTTTCCCTCCGCTGATTTCCATCGAGAAGAAG
NM_000681.3       ACCGTGTGGGTCATCTCGGCCGTCATCTCCTTCCCGCCGCTCATCTCCATCGAGAAGAAG
                  ***** *********** ** ** ***** ***** ***** ** ***************

HsCD00292909      GGTGGCGGTGGTGGTCCACAGCCTGCTGAACCGCGTTGCGAAATCAATGATCAGAAGTGG
NM_000681.3       GGCGGCGGCGGCGGCCCGCAGCCGGCCGAGCCGCGCTGCGAGATCAACGACCAGAAGTGG
                  ** ***** ** ** **.***** ** **.***** *****.***** ** *********

HsCD00292909      TACGTTATTAGCAGCTGTATTGGTTCTTTCTTTGCCCCATGCCTGATTATGATCCTGGTG
NM_000681.3       TACGTCATCTCGTCGTGCATCGGCTCCTTCTTCGCTCCCTGCCTCATCATGATCCTGGTC
                  ***** ** :  :  ** ** ** ** ***** ** **.***** ** *********** 

HsCD00292909      TACGTGCGTATCTATCAGATCGCCAAACGCCGTACCCGCGTTCCGCCGTCCCGCCGTGGC
NM_000681.3       TACGTGCGCATCTACCAGATCGCCAAGCGTCGCACCCGCGTGCCACCCAGCCGCCGGGGT
                  ******** ***** ***********.** ** ******** **.** : ****** ** 

HsCD00292909      CCGGACGCTGTGGCGGCTCCGCCTGGTGGTACCGAGCGTCGTCCGAACGGCCTGGGCCCA
NM_000681.3       CCGGACGCCGTCGCCGCGCCGCCGGGGGGCACCGAGCGCAGGCCCAACGGTCTGGGCCCC
                  ******** ** ** ** ***** ** ** ******** .* ** ***** ********.

HsCD00292909      GAACGTAGCGCAGGCCCGGGTGGCGCGGAAGCCGAACCGCTGCCGACCCAGCTGAACGGT
NM_000681.3       GAGCGCAGCGCGGGCCCGGGGGGCGCAGAGGCCGAACCGCTGCCCACCCAGCTCAACGGC
                  **.** *****.******** *****.**.************** ******** ***** 

HsCD00292909      GCGCCTGGTGAACCAGCCCCGGCTGGTCCACGCGATACTGACGCGCTGGATCTGGAGGAG
NM_000681.3       GCCCCTGGCGAGCCCGCGCCGGCCGGGCCGCGCGACACCGACGCGCTGGACCTGGAGGAG
                  ** ***** **.**.** ***** ** **.***** ** *********** *********

HsCD00292909      TCCTCTTCCTCTGACCACGCGGAGCGTCCGCCTGGCCCGCGTCGTCCAGAGCGTGGTCCA
NM_000681.3       AGCTCGTCTTCCGACCACGCCGAGCGGCCTCCAGGGCCCCGCAGACCCGAGCGCGGTCCC
                  : *** ** ** ******** ***** ** **:** ** ** .*:**.***** *****.

HsCD00292909      CGTGGTAAAGGCAAAGCCCGCGCTTCCCAGGTTAAGCCGGGTGATAGCCTGCCTCGCCGT
NM_000681.3       CGGGGCAAAGGCAAGGCCCGAGCGAGCCAGGTGAAGCCGGGCGACAGCCTGCCGCGGCGC
                  ** ** ********.*****.** : ****** ******** ** ******** ** ** 

HsCD00292909      GGCCCTGGTGCCACTGGTATTGGCACGCCAGCGGCGGGTCCGGGCGAAGAACGTGTAGGC
NM_000681.3       GGGCCGGGGGCGACGGGGATCGGGACGCCGGCTGCAGGGCCGGGGGAGGAGCGCGTCGGG
                  ** ** ** ** ** ** ** ** *****.** **.** ***** **.**.** **.** 

HsCD00292909      GCAGCGAAGGCATCCCGTTGGCGTGGTCGCCAGAACCGTGAGAAGCGTTTCACGTTCGTC
NM_000681.3       GCTGCCAAGGCGTCGCGCTGGCGCGGGCGGCAGAACCGCGAGAAGCGCTTCACGTTCGTG
                  **:** *****.** ** ***** ** ** ******** ******** *********** 

HsCD00292909      CTGGCGGTGGTAATTGGTGTATTTGTAGTGTGTTGGTTCCCGTTCTTTTTCACCTACACC
NM_000681.3       CTGGCCGTGGTCATCGGAGTGTTCGTGGTGTGCTGGTTCCCCTTCTTCTTCACCTACACG
                  ***** *****.** **:**.** **.***** ******** ***** *********** 

HsCD00292909      CTGACTGCCGTGGGTTGCTCCGTGCCACGCACTCTGTTCAAATTCTTTTTCTGGTTCGGC
NM_000681.3       CTCACGGCCGTCGGGTGCTCCGTGCCACGCACGCTCTTCAAATTCTTCTTCTGGTTCGGC
                  ** ** ***** ** ***************** ** *********** ************

HsCD00292909      TACTGTAACTCTTCCCTGAACCCGGTTATCTATACTATCTTCAACCACGATTTTCGTCGT
NM_000681.3       TACTGCAACAGCTCGTTGAACCCGGTCATCTACACCATCTTCAACCACGATTTCCGCCGC
                  ***** ***:  **  ********** ***** ** ***************** ** ** 

HsCD00292909      GCGTTCAAAAAGATCCTGTGTCGCGGTGATCGTAAACGCATCGTGCACCACCATCACCAT
NM_000681.3       GCCTTCAAGAAGATCCTCTGTCGGGGGGACAGGAAGCGGATCGTGTGA------------
                  ** *****.******** ***** ** ** .* **.** ****** ..            

HsCD00292909      CACCATCACCATCACTGAAAGCTTCTCGAG
NM_000681.3       ------------------------------

Pathways

Panther :

Alpha adrenergic receptor signaling pathway

Reactome :

Adrenaline signalling through Alpha-2 adrenergic receptor

Reactome :

Adrenaline,noradrenaline inhibits insulin secretion

Reactome :

Adrenoceptors

Reactome :

Amine ligand-binding receptors

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (i) signalling events

Reactome :

G alpha (z) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Hemostasis

Reactome :

Integration of energy metabolism

Reactome :

Metabolism

Reactome :

Platelet activation, signaling and aggregation

Reactome :

Platelet Aggregation (Plug Formation)

Reactome :

Regulation of insulin secretion

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

WikiPathway :

Monoamine GPCRs

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

P08913

HPRD :

00078

Clone

Cloning Information :

485273

HIP Master Clone ID :

401830

Original Clone ID :

ATCG3D_13

Experimental

TargetTrack - Experimental data :

ATCG3D_1

TargetTrack - Experimental data :

GPCR-10

Organism

Species Specific ID:

150

Genome

Chromosome :

Map Location :

10q25.2

Ensembl :

ENSG00000150594

Reagents

Labome :

ADRA2A-antibody