Gene:
Gene Symbol:
ADORA2A
Gene Name:
adenosine A2a receptor
Original Clone ID:
ATCG3D_29
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJ2
Format:
CLOSED
Source:
GPCR Network
Description:
G-protein coupled receptor
Comments:
Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA2.0 for Expression in BV only. Supplied in the Vendor's Vector.
Mutation / Discrepancy:
No / No
Publications:
Authors:
PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
HsCD00292915
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
GGATCCATGAAGACAATTATCGCTTTGAGTTACATATTCTGTTTGGTTTTTGCGGACTAC
AAGGATGACGACGATATGCCGATCATGGGATCGTCAGTTTACATCACCGTTGAACTTGCT
ATAGCTGTACTCGCTATCCTTGGTAACGTACTCGTTTGCTGGGCGGTGTGGCTCAACTCG
AACCTTCAGAACGTCACAAATTACTTCGTAGTCTCTCTGGCGGCTGCGGACATTGCGGTA
GGCGTTCTCGCCATCCCGTTCGCCATCACTATCTCTACCGGTTTCTGTGCCGCTTGCCAC
GGCTGCCTCTTTATTGCTTGCTTCGTTTTGGTACTTACCCAATCTTCTATCTTCTCGCTC
TTGGCTATCGCGATCGACAGATACATAGCTATCCGTATCCCTCTGAGGTACAATGGACTG
GTGACAGGCACACGTGCTAAGGGCATCATCGCTATCTGCTGGGTATTGTCATTCGCTATC
GGACTGACTCCAATGCTGGGCTGGAACAACTGTGGACAGCCTAAGGAAGGAAAGAACCAC
TCTCAAGGATGTGGCGAAGGTCAAGTCGCGTGCCTCTTTGAAGACGTAGTGCCGATGAAC
TATATGGTCTACTTCAACTTCTTTGCTTGCGTCCTGGTGCCGCTGCTCTTGATGCTTGGT
GTATACCTCCGTATCTTCCTTGCCGCTAGACGTCAGCTTAAACAGATGGAGTCTCAACCT
CTTCCTGGAGAGAGAGCGAGGTCAACTTTGCAGAAGGAGGTACATGCCGCCAAGTCATTG
GCTATCATCGTCGGTCTTTTTGCGCTGTGCTGGCTGCCCCTCCACATCATCAACTGTTTT
ACCTTCTTCTGTCCCGACTGTTCACACGCACCTCTTTGGCTGATGTACCTGGCGATCGTT
CTCAGCCACACCAATTCGGTCGTAAATCCGTTTATCTATGCTTACCGTATTCGTGAATTT
CGCCAGACGTTTCGCAAGATCATCAGGTCCCATGTCCTCAGACAGCAAGAACCTTTCAAG
GCTGCCGGCACCTCCGCCAGGGTCTTGGCGGCGCACGGAAGCGACGGTGAGCAAGTGTCT
TTGCGCCTTAACGGACACCCTCCCGGTGTCTGGGCGAACGGCTCTGCCCCACACCCCGAG
AGACGCCCCAATGGTTACGCTCTGGGACTTGTGTCTGGCGGATCAGCTCAGGAAAGCCAA
GGCAACACTGGTTTGCCTGACGTCGAACTGCTGTCGCACGAGTTGAAGGGCGTTTGCCCG
GAGCCGCCTGGCTTGGACGACCCCTTGGCACAGGACGGTGCTGGCGTTTCGCACCACCAT
CACCATCACCATCACCATCACTGAAAGCTTCTCGAG
Protein sequence predicted based on nucleotide sequence
MKTIIALSYIFCLVFADYKDDDDMPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNL QNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLA IAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQ GCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLP GERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLS HTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLR LNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEP PGLDDPLAQDGAGVSHHHHHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
kanamycin
Growth Condition:
Growth in 30 ug/ml of kanamycin in LB at 37 degrees
Comments:
None
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in ATCG3D_5
Find experimental protocols in GPCR-6
Recommended expression in:
Not Applicable
Vector Name:
pJ2
Synonyms:
''
Description:
Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.
Comments:
Supplied by DNA2.0
Size (bp):
2213
Parent Vector:
None
Properties:
TOPO Cloning
Author Name:
DNA2.0
Accelerated Technologies Center for Gene to 3D Structure
Accelerated Technologies Center for Gene to 3D Structure
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pUC ori
Bacterial origin of replication
0
0
primer
M13 forward
M13 forward sequencing primer
2062
2077
primer
M13 reverse
M13 reverse sequencing primer
114
131
selectable marker
KanR
Kanamycin resistance gene
268
1050
