DNASU Plasmid | HTR1A (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: HTR1A

Gene Name: 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

Original Clone ID:

ATCG3D_3

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

GPCR Network

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered in Gene Composer for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network

HsCD00292931

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1368nts

Open reading frame: 7 to 1374

Reference Sequence Alignment

GGATCCATGAAGACCATCATCGCTCTGTCCTATATCTTTTGTCTGGTGTTTGCCGACTAC
AAAGACGATGACGATATGGACGTTCTTTCACCTGGTCAAGGTAACAACACCACCTCACCT
CCCGCACCATTTGAGACAGGCGGCAACACCACTGGCATCTCAGACGTGACAGTCTCCTAC
CAGGTGATCACGAGCCTTCTCCTGGGTACCCTGATCTTCTGCGCTGTCCTCGGGAACGCT
TGCGTGGTGGCTGCAATCGCCCTGGAGCGCAGTCTTCAGAACGTGGCCAACTACCTCATC
GGTTCCCTGGCCGTCACGGACCTGATGGTGTCCGTTCTGGTCCTCCCTATGGCAGCCCTG
TACCAGGTCCTCAACAAGTGGACTCTGGGCCAAGTGACATGTGATCTGTTTATCGCACTC
GACGTGCTGTGTTGTACTTCAAGCATCCTCCACCTGTGCGCTATCGCCCTGGATCGCTAT
TGGGCTATTACCGACCCTATCGACTACGTGAACAAGCGCACCCCTCGTCGCGCCGCTGCC
CTGATTTCCCTGACCTGGCTGATCGGTTTCCTGATCAGCATCCCTCCAATGCTCGGGTGG
CGTACGCCGGAGGACCGCAGCGATCCTGATGCTTGTACCATCTCTAAGGACCACGGCTAT
ACCATCTACTCCACGTTCGGGGCCTTCTATATCCCACTGCTCCTGATGCTTGTCCTCTAC
GGCCGTATCTTCCGTGCTGCTCGCTTCCGCATCCGCAAAACCGTTAAGAAAGTCGAGAAA
ACAGGCGCCGACACCCGCCACGGAGCCTCTCCCGCTCCCCAGCCTAAGAAGAGTGTCAAC
GGCGAGTCTGGGTCCCGTAACTGGCGTCTGGGTGTTGAGAGCAAAGCTGGAGGCGCACTC
TGTGCAAACGGGGCTGTGCGTCAAGGCGATGACGGCGCCGCTCTGGAGGTCATTGAGGTC
CACCGTGTCGGCAATAGCAAGGAGCACCTGCCTCTTCCCAGCGAGGCTGGCCCGACCCCT
TGTGCCCCTGCATCTTTCGAACGCAAGAACGAGCGCAACGCCGAAGCCAAGCGCAAGATG
GCCCTGGCTCGCGAACGCAAGACCGTTAAGACCCTGGGTATTATTATGGGCACGTTCATT
CTCTGTTGGCTGCCATTCTTTATTGTTGCTCTGGTTCTTCCGTTCTGCGAGAGTTCCTGT
CACATGCCCACTCTGCTCGGGGCAATTATTAATTGGCTGGGGTACTCAAATTCACTGCTG
AACCCCGTCATCTATGCATACTTCAATAAGGACTTTCAGAATGCATTCAAGAAAATCATC
AAGTGTAAATTTTGCCGTCAGCACCACCATCACCATCACCATCACCATCACTGAAAGCTT
CTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQV
ITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQ
VLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALI
SLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGR
IFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCA
NGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMAL
ARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNP
VIYAYFNKDFQNAFKKIIKCKFCRQHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: kanamycin

Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in GPCR-1

Find experimental protocols in ATCG3D_3

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

HTR1A

Symbol Nomenclature :

HTR1A

SYNONYM :

5-HT-1A; 5-HT1A; 5HT1a; ADRB2RL1; ADRBRL1; G-21; PFMCD

Designation :

5-HT1a receptor|5-hydroxytryptamine receptor 1A|guanine nucleotide-binding regulatory protein-coupled receptor

Full Nomenclature :

5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

GENEID :

3350

HGNC :

5286

MIM :

109760

Target GenBank:

NP_000515

Reference Sequence Alignment

  

HsCD00292931      GGATCCATGAAGACCATCATCGCTCTGTCCTATATCTTTTGTCTGGTGTTTGCCGACTAC
NM_000524.3       ------------------------------------------------------------
                                                                              

HsCD00292931      AAAGACGATGACGATATGGACGTTCTTTCACCTGGTCAAGGTAACAACACCACCTCACCT
NM_000524.3       ---------------ATGGATGTGCTCAGCCCTGGTCAGGGCAACAACACCACATCACCA
                                 ***** ** ** : .********.** ***********.*****:

HsCD00292931      CCCGCACCATTTGAGACAGGCGGCAACACCACTGGCATCTCAGACGTGACAGTCTCCTAC
NM_000524.3       CCGGCTCCCTTTGAGACCGGCGGCAACACTACTGGTATCTCCGACGTGACCGTCAGCTAC
                  ** **:**.********.*********** ***** *****.********.***: ****

HsCD00292931      CAGGTGATCACGAGCCTTCTCCTGGGTACCCTGATCTTCTGCGCTGTCCTCGGGAACGCT
NM_000524.3       CAAGTGATCACCTCTCTGCTGCTGGGCACGCTCATCTTCTGCGCGGTGCTGGGCAATGCG
                  **.******** :  ** ** ***** ** ** *********** ** ** ** ** ** 

HsCD00292931      TGCGTGGTGGCTGCAATCGCCCTGGAGCGCAGTCTTCAGAACGTGGCCAACTACCTCATC
NM_000524.3       TGCGTGGTGGCTGCCATCGCCTTGGAGCGCTCCCTGCAGAACGTGGCCAATTATCTTATT
                  **************.****** ********:  ** ************** ** ** ** 

HsCD00292931      GGTTCCCTGGCCGTCACGGACCTGATGGTGTCCGTTCTGGTCCTCCCTATGGCAGCCCTG
NM_000524.3       GGCTCTTTGGCGGTCACCGACCTCATGGTGTCGGTGTTGGTGCTGCCCATGGCCGCGCTG
                  ** **  **** ***** ***** ******** **  **** ** ** *****.** ***

HsCD00292931      TACCAGGTCCTCAACAAGTGGACTCTGGGCCAAGTGACATGTGATCTGTTTATCGCACTC
NM_000524.3       TATCAGGTGCTCAACAAGTGGACACTGGGCCAGGTAACCTGCGACCTGTTCATCGCCCTC
                  ** ***** **************:********.**.**.** ** ***** *****.***

HsCD00292931      GACGTGCTGTGTTGTACTTCAAGCATCCTCCACCTGTGCGCTATCGCCCTGGATCGCTAT
NM_000524.3       GACGTGCTGTGCTGCACCTCATCCATCTTGCACCTGTGCGCCATCGCGCTGGACAGGTAC
                  *********** ** ** ***: **** * *********** ***** ***** .* ** 

HsCD00292931      TGGGCTATTACCGACCCTATCGACTACGTGAACAAGCGCACCCCTCGTCGCGCCGCTGCC
NM_000524.3       TGGGCCATCACGGACCCCATCGACTACGTGAACAAGAGGACGCCCCGGCGCGCCGCTGCG
                  ***** ** ** ***** ******************.* ** ** ** *********** 

HsCD00292931      CTGATTTCCCTGACCTGGCTGATCGGTTTCCTGATCAGCATCCCTCCAATGCTCGGGTGG
NM_000524.3       CTCATCTCGCTCACTTGGCTTATTGGCTTCCTCATCTCTATCCCGCCCATGCTGGGCTGG
                  ** ** ** ** ** ***** ** ** ***** ***:  ***** **.***** ** ***

HsCD00292931      CGTACGCCGGAGGACCGCAGCGATCCTGATGCTTGTACCATCTCTAAGGACCACGGCTAT
NM_000524.3       CGCACCCCGGAAGACCGCTCGGACCCCGACGCATGCACCATTAGCAAGGATCATGGCTAC
                  ** ** *****.******:  ** ** ** **:** ***** :  ***** ** ***** 

HsCD00292931      ACCATCTACTCCACGTTCGGGGCCTTCTATATCCCACTGCTCCTGATGCTTGTCCTCTAC
NM_000524.3       ACTATCTATTCCACCTTTGGAGCTTTCTACATCCCGCTGCTGCTCATGCTGGTTCTCTAT
                  ** ***** ***** ** **.** ***** *****.***** ** ***** ** ***** 

HsCD00292931      GGCCGTATCTTCCGTGCTGCTCGCTTCCGCATCCGCAAAACCGTTAAGAAAGTCGAGAAA
NM_000524.3       GGGCGCATATTCCGAGCTGCGCGCTTCCGCATCCGCAAGACGGTCAAAAAGGTGGAGAAG
                  ** ** **.*****:***** *****************.** ** **.**.** *****.

HsCD00292931      ACAGGCGCCGACACCCGCCACGGAGCCTCTCCCGCTCCCCAGCCTAAGAAGAGTGTCAAC
NM_000524.3       ACCGGAGCGGACACCCGCCATGGAGCATCTCCCGCCCCGCAGCCCAAGAAGAGTGTGAAT
                  **.**.** *********** *****.******** ** ***** *********** ** 

HsCD00292931      GGCGAGTCTGGGTCCCGTAACTGGCGTCTGGGTGTTGAGAGCAAAGCTGGAGGCGCACTC
NM_000524.3       GGAGAGTCGGGGAGCAGGAACTGGAGGCTGGGCGTGGAGAGCAAGGCTGGGGGTGCTCTG
                  **.***** ***: *.* ******.* ***** ** ********.*****.** **:** 

HsCD00292931      TGTGCAAACGGGGCTGTGCGTCAAGGCGATGACGGCGCCGCTCTGGAGGTCATTGAGGTC
NM_000524.3       TGCGCCAATGGCGCGGTGAGGCAAGGTGACGATGGCGCCGCCCTGGAGGTGATCGAGGTG
                  ** **.** ** ** ***.* ***** ** ** ******** ******** ** ***** 

HsCD00292931      CACCGTGTCGGCAATAGCAAGGAGCACCTGCCTCTTCCCAGCGAGGCTGGCCCGACCCCT
NM_000524.3       CACCGAGTGGGCAACTCCAAAGAGCACTTGCCTCTGCCCAGCGAGGCTGGTCCTACCCCT
                  *****:** ***** : ***.****** ******* ************** ** ******

HsCD00292931      TGTGCCCCTGCATCTTTCGAACGCAAGAACGAGCGCAACGCCGAAGCCAAGCGCAAGATG
NM_000524.3       TGTGCCCCCGCCTCTTTCGAGAGGAAAAATGAGCGCAACGCCGAGGCGAAGCGCAAGATG
                  ******** **.********..* **.** **************.** ************

HsCD00292931      GCCCTGGCTCGCGAACGCAAGACCGTTAAGACCCTGGGTATTATTATGGGCACGTTCATT
NM_000524.3       GCCCTGGCCCGAGAGAGGAAGACAGTGAAGACGCTGGGCATCATCATGGGCACCTTCATC
                  ******** **.**..* *****.** ***** ***** ** ** ******** ***** 

HsCD00292931      CTCTGTTGGCTGCCATTCTTTATTGTTGCTCTGGTTCTTCCGTTCTGCGAGAGTTCCTGT
NM_000524.3       CTCTGCTGGCTGCCCTTCTTCATCGTGGCTCTTGTTCTGCCCTTCTGCGAGAGCAGCTGC
                  ***** ********.***** ** ** ***** ***** ** *********** : *** 

HsCD00292931      CACATGCCCACTCTGCTCGGGGCAATTATTAATTGGCTGGGGTACTCAAATTCACTGCTG
NM_000524.3       CACATGCCCACCCTGTTGGGCGCCATAATCAATTGGCTGGGCTACTCCAACTCTCTGCTT
                  *********** *** * ** **.**:** *********** *****.** **:***** 

HsCD00292931      AACCCCGTCATCTATGCATACTTCAATAAGGACTTTCAGAATGCATTCAAGAAAATCATC
NM_000524.3       AACCCCGTCATTTACGCATACTTCAACAAGGACTTTCAAAACGCGTTTAAGAAGATCATT
                  *********** ** *********** ***********.** **.** *****.***** 

HsCD00292931      AAGTGTAAATTTTGCCGTCAGCACCACCATCACCATCACCATCACCATCACTGAAAGCTT
NM_000524.3       AAGTGTAAGTTCTGCCGCCAGTGA------------------------------------
                  ********.** ***** *** ..                                    

HsCD00292931      CTCGAG
NM_000524.3       ------

Pathways

Panther :

5HT1 type receptor mediated signaling pathway

Panther :

Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway

Reactome :

Amine ligand-binding receptors

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (i) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Serotonin receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

WikiPathway :

Monoamine GPCRs

WikiPathway :

Serotonin HTR1 Group and FOS Pathway

WikiPathway :

SIDS Susceptibility Pathways

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

Q5ZGX3

UniProt :

P08908

HPRD :

00190

Clone

Cloning Information :

485295

HIP Master Clone ID :

401852

Original Clone ID :

ATCG3D_3

Experimental

TargetTrack - Experimental data :

GPCR-1

TargetTrack - Experimental data :

ATCG3D_3

Organism

Species Specific ID:

3350

Genome

Chromosome :

Map Location :

5q11.2-q13

Reagents

Labome :

HTR1A-antibody