ADRB2 (H. sapiens )
In pET28a (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  ADRB2
Gene Name:  adrenoceptor beta 2, surface
Original Clone ID:
GPCR-15
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET28a
Format: 
CLOSED
Source:
GPCR Network
Description:
G-protein coupled receptor
Comments:
Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. ATCG3D-19 Cloned into our pET28a Expression Vector.
Mutation / Discrepancy:
No / No
Publications:
PMID: 17962520
Title: High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.
PMID: 18547522
Title: A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor.
PMID: 23574807
Title: Synthesis, antidepressant evaluation and docking studies of long-chain alkylnitroquipazines as serotonin transporter inhibitors.
Authors:
PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
HsCD00292938
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/T7
Insert sequence: 1260nts
Open reading frame: 76 to 1335

GGATCCATGAAGACTATTATCGCTCTGTCCTACATTTTCTGCCTGGTTTTCGCTGACTAT
AAGGACGACGACGACATGGGTCAACCGGGTAATGGTTCTGCGTTTCTGCTGGCTCCGAAC
CGCTCTCACGCACCGGATCATGACGTGACTCAACAGCGTGATGAAGTGTGGGTGGTGGGT
ATGGGTATCGTGATGTCCCTGATCGTGCTGGCCATCGTATTTGGTAACGTACTGGTGATC
ACGGCCATTGCCAAATTCGAACGTCTGCAAACTGTAACCAATTACTTCATTACCTCTCTG
GCGTGTGCTGACCTGGTCATGGGCCTGGCTGTTGTGCCATTTGGCGCGGCTCATATCCTG
ATGAAGATGTGGACCTTCGGTAACTTCTGGTGCGAATTCTGGACCAGCATCGATGTCCTG
TGCGTTACCGCTTCCATCGAAACCCTGTGTGTGATCGCTGTTGACCGCTACTTCGCTATT
ACCTCCCCGTTCAAGTACCAGTCCCTGCTGACTAAGAACAAAGCTCGCGTCATCATCCTG
ATGGTCTGGATTGTTTCCGGTCTGACTTCCTTTCTGCCGATCCAGATGCATTGGTACCGC
GCAACCCATCAGGAGGCTATTAATTGCTACGCCGAAGAAACTTGCTGTGATTTCTTTACC
AACCAGGCTTACGCCATCGCATCTTCTATCGTTTCTTTCTACGTACCGCTGGTGATCATG
GTATTCGTTTACTCCCGTGTGTTCCAAGAGGCGAAACGTCAACTGCAGAAGATCGATAAA
TCTGAAGGTCGCTTTCACGTCCAGAACCTGTCCCAGGTCGAACAAGATGGTCGTACGGGT
CATGGCCTGCGCCGCTCCAGCAAGTTCTGTCTGAAAGAGCATAAGGCGCTGAAAACTCTG
GGCATCATCATGGGCACTTTCACCCTGTGCTGGCTGCCGTTCTTCATCGTAAACATTGTT
CATGTAATCCAAGACAATCTGATCCGCAAGGAAGTTTATATTCTGCTGAACTGGATCGGT
TATGTCAATTCCGGTTTCAACCCGCTGATCTACTGTCGTTCCCCAGACTTTCGCATTGCA
TTCCAGGAACTGCTGTGTCTGCGTCGCAGCTCCCTGAAGGCATACGGCAACGGTTATAGC
TCCAACGGCAACACCGGTGAACAGAGCGGTTACCATGTGGAGCAGGAGAAGGAAAACAAA
CTGCTGTGTGAAGACCTGCCGGGCACTGAGGACTTTGTCGGCCACCAGGGCACTGTGCCG
TCCGACAACATCGACTCCCAGGGCCGCAACTGCTCCACTAACGACTCTCTGCTGCACCAT
CACCACCACCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAK
FERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTAS
IETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQE
AINCYAEETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRF
HVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQD
NLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNT
GEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLLHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in ATCG3D_7
Find experimental protocols in GPCR-15
Recommended expression in:
Not Applicable