ADORA2A (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:
Gene Symbol:  ADORA2A
Gene Name:  adenosine A2a receptor
Original Clone ID:
ATCG3D_5
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pJ2
Format: 
CLOSED
Source:
GPCR Network
Description:
G-protein coupled receptor
Comments:
Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered in Gene Composer for Expression in E. coli and BV. Supplied in the Vendor's Vector.
Mutation / Discrepancy:
No / No
Publications:
PMID: 18832607
Title: The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist.
PMID: 21393508
Title: Structure of an agonist-bound human A2A adenosine receptor.
Authors:
PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
HsCD00292939
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1338nts
Open reading frame: 7 to 1344

GGATCCATGAAGACCATCATTGCACTTAGTTATATTTTCTGCCTTGTTTTCGCCGATTAT
AAGGACGATGACGATATGCCGATTATGGGTTCATCCGTTTACATCACAGTTGAACTTGCA
ATCGCAGTGCTTGCTATCCTGGGGAACGTGCTGGTGTGCTGGGCTGTGTGGCTGAACTCC
AATCTGCAAAACGTGACTAATTATTTCGTGGTGAGCCTGGCCGCTGCCGATATCGCTGTC
GGCGTCCTGGCTATCCCGTTTGCTATCACAATTAGCACCGGCTTCTGTGCCGCCTGCCAC
GGCTGTCTGTTCATTGCTTGCTTTGTTCTGGTGCTGACGCAGAGTTCTATCTTTAGCCTG
CTCGCCATCGCTATCGATCGCTACATCGCCATTCGTATCCCTCTCCGTTACAATGGCCTC
GTCACTGGCACCCGTGCCAAGGGAATCATTGCAATCTGTTGGGTGCTGAGCTTCGCAATC
GGCCTCACCCCAATGCTGGGCTGGAATAACTGCGGTCAGCCTAAAGAGGGAAAGAACCAT
TCACAGGGCTGCGGCGAAGGTCAGGTTGCTTGCCTGTTTGAAGACGTCGTGCCCATGAAC
TATATGGTTTACTTTAACTTCTTTGCATGCGTGCTGGTGCCGCTGCTCCTGATGCTGGGT
GTGTATCTGCGTATTTTCCTTGCTGCTCGTCGCCAGCTTAAGCAAATGGAAAGTCAACCC
CTGCCCGGCGAACGCGCCCGCAGCACCCTCCAGAAGGAGGTCCACGCTGCTAAGTCACTT
GCCATCATTGTGGGTCTCTTTGCCCTTTGTTGGCTGCCACTTCACATTATCAACTGTTTC
ACTTTCTTCTGTCCAGATTGTAGCCACGCTCCACTGTGGCTGATGTACCTCGCTATCGTC
CTGAGCCACACAAACAGTGTTGTCAACCCCTTTATCTACGCATACCGCATTCGCGAATTT
CGCCAGACCTTCCGCAAGATCATTCGTAGCCATGTGCTTCGCCAGCAGGAGCCCTTTAAG
GCAGCTGGCACGAGCGCCCGTGTTCTTGCTGCTCATGGAAGTGACGGCGAGCAGGTGAGC
CTCCGTCTCAACGGGCATCCTCCCGGCGTTTGGGCCAACGGCAGCGCTCCTCATCCTGAG
CGTCGCCCTAACGGCTATGCCCTCGGTCTGGTGAGCGGAGGGTCAGCTCAAGAATCCCAG
GGCAACACCGGACTGCCAGACGTCGAGCTGCTGTCCCATGAGCTGAAAGGTGTGTGTCCG
GAACCTCCCGGACTTGACGATCCTCTCGCTCAGGACGGCGCCGGCGTGTCCCACCACCAT
CACCATCACCATCACCATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNL
QNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLA
IAIDRYIAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQ
GCGEGQVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLP
GERARSTLQKEVHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLS
HTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLR
LNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEP
PGLDDPLAQDGAGVSHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: kanamycin
Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees
Comments: None
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GPCR-6
Find experimental protocols in ATCG3D_5
Recommended expression in:
Not Applicable