Gene:
Gene Symbol:
ADRB2
Gene Name:
adrenoceptor beta 2, surface
Original Clone ID:
ATCG3D_31
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET28a
Format:
CLOSED
Source:
GPCR Network
Description:
G-protein coupled receptor
Comments:
Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA2.0 for Expression in BV only. Supplied in the Vendor's Vector.
Mutation / Discrepancy:
No / No
Publications:
PMID: 17962520
Title: High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.
PMID: 18547522
Title: A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor.
PMID: 23574807
Title: Synthesis, antidepressant evaluation and docking studies of long-chain alkylnitroquipazines as serotonin transporter inhibitors.
Title: High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor.
PMID: 18547522
Title: A specific cholesterol binding site is established by the 2.8 A structure of the human beta2-adrenergic receptor.
PMID: 23574807
Title: Synthesis, antidepressant evaluation and docking studies of long-chain alkylnitroquipazines as serotonin transporter inhibitors.
Authors:
PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network
HsCD00292946
Price:
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AVAILABLE
No restriction
Special MTA: PSI/T7
Special MTA: PSI/T7
GGATCCATGAAAACCATTATCGCTTTGAGCTATATCTTCTGCCTCGTCTTTGCAGATTAC
AAGGACGACGACGACATGGGCCAGCCTGGAAACGGCAGCGCTTTCTTGCTCGCCCCCAAC
AGGTCACACGCACCCGACCATGACGTGACCCAACAAAGAGACGAGGTGTGGGTCGTCGGC
ATGGGTATAGTGATGAGCCTTATCGTTCTGGCTATAGTCTTTGGAAACGTTCTTGTCATT
ACCGCCATTGCAAAGTTCGAAAGATTGCAAACTGTCACAAATTACTTCATAACTTCACTT
GCATGTGCGGATCTGGTCATGGGTCTGGCCGTTGTACCTTTCGGAGCCGCGCATATTCTG
ATGAAGATGTGGACTTTCGGTAATTTCTGGTGTGAATTCTGGACTAGCATTGACGTTCTG
TGCGTCACAGCAAGTATCGAAACGTTGTGCGTCATCGCTGTGGACCGTTATTTCGCCATT
ACAAGCCCGTTTAAATACCAGTCCCTGCTCACAAAGAATAAGGCCCGTGTCATCATCCTT
ATGGTCTGGATTGTCTCCGGTCTGACCTCGTTTCTGCCAATTCAAATGCACTGGTACAGA
GCCACCCACCAGGAGGCTATTAATTGCTACGCTGAGGAAACCTGCTGTGATTTCTTTACT
AACCAGGCTTACGCAATTGCTTCCTCTATAGTCTCTTTCTATGTTCCCCTTGTCATTATG
GTGTTTGTGTATTCTCGTGTTTTCCAGGAAGCAAAGCGCCAGCTTCAGAAGATCGACAAG
TCTGAGGGCAGGTTTCATGTTCAGAACCTGAGTCAGGTAGAGCAAGACGGTAGGACTGGA
CATGGTTTGCGCAGGTCATCAAAGTTTTGCCTCAAAGAGCACAAGGCCCTCAAGACACTG
GGCATTATCATGGGTACTTTCACATTGTGTTGGTTGCCCTTTTTCATCGTCAATATTGTG
CACGTTATTCAAGACAATCTGATACGTAAGGAGGTGTATATCCTGCTCAACTGGATCGGC
TACGTCAATAGCGGATTCAACCCCCTCATCTACTGCCGTTCCCCTGACTTCAGGATTGCG
TTCCAGGAACTTCTTTGCTTGCGTAGGTCCTCGCTGAAGGCTTACGGAAACGGATATTCA
TCAAATGGTAACACTGGAGAGCAAAGCGGTTACCATGTGGAGCAGGAGAAAGAGAACAAA
CTGCTGTGCGAGGACCTCCCAGGTACAGAAGACTTCGTCGGCCATCAGGGTACTGTCCCT
TCGGACAACATCGATTCACAGGGACGTAACTGCTCCACGAACGACTCTTTGCTTCATCAC
CACCACCATCATTGAAAGCTTCTCGAG
Protein sequence predicted based on nucleotide sequence
MGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAK FERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTAS IETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQE AINCYAEETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRF HVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQD NLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNT GEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLLHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in GPCR-15
Find experimental protocols in ATCG3D_7
Recommended expression in:
Not Applicable
Vector Name:
pET28a
Synonyms:
pET-28a, pET28
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7lac promoter, adds N-terminal His tag, thrombin cleavage site, internal T7 epitope tag, C-terminal His tag; kanamycin resistance; restriction enzyme cloning.
Comments:
None
Size (bp):
5369
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
Midwest Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (XhoI, NotI, EagI, HindIII, SalI, SacI, EcoRI, BamHI, NheI, NdeI, NcoI)
158
203
bacterial origin
f1
f1 origin
4903
5358
bacterial origin
ori
bacterial origin of replication
3270
3889
promoter
T7
T7 promoter
368
386
repressor protein gene
LacI
lacI coding sequence (lacI cds)
896
1852
selectable marker
KanR
kanamycin resistance
3995
4807
tag
His
6xHis tag
140
157
trxn regulatory element
lac operator
lac operator
347
363
trxn termination sequence
T7 term
T7 terminator
26
72
