DNASU Plasmid | HTR1A (Homo sapiens) in pJ4 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: HTR1A

Gene Name: 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

Original Clone ID:

GPCR-1

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ4

Format:

CLOSED

Source:

GPCR Network

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered by DNA 2.0 for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network

HsCD00292952

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1368nts

Open reading frame: 7 to 1374

Reference Sequence Alignment

GGATCCATGAAGACTATCATTGCTCTGTCCTATATTTTCTGTCTGGTGTTCGCCGATTAC
AAGGATGACGACGATATGGATGTCCTGTCTCCGGGCCAAGGTAACAACACGACCTCTCCT
CCGGCGCCATTCGAAACCGGTGGTAACACCACTGGTATCTCTGACGTAACCGTTTCTTAC
CAGGTAATCACCTCCCTGCTGCTGGGTACCCTGATTTTCTGTGCTGTTCTGGGCAATGCA
TGTGTTGTAGCGGCTATTGCACTGGAACGTAGCCTGCAGAACGTCGCTAATTATCTGATT
GGCTCTCTGGCAGTAACCGATCTGATGGTATCCGTACTGGTCCTGCCAATGGCAGCGCTG
TATCAGGTTCTGAACAAGTGGACGCTGGGCCAAGTAACTTGTGATCTGTTCATTGCGCTG
GACGTTCTGTGCTGCACCAGCAGCATCCTGCACCTGTGCGCTATCGCTCTGGACCGCTAC
TGGGCCATCACCGACCCGATCGATTACGTGAACAAACGTACTCCGCGCCGTGCTGCCGCC
CTGATCTCTCTGACGTGGCTGATCGGTTTCCTGATCAGCATCCCTCCGATGCTGGGCTGG
CGTACCCCTGAGGATCGTTCTGACCCTGACGCTTGCACGATTAGCAAGGACCACGGCTAC
ACCATCTACTCCACTTTCGGTGCTTTCTATATCCCTCTGCTGCTGATGCTGGTACTGTAC
GGCCGTATCTTCCGTGCTGCCCGCTTTCGCATCCGCAAAACCGTGAAGAAAGTCGAGAAA
ACCGGTGCGGATACCCGTCATGGTGCGTCTCCGGCACCGCAACCGAAGAAATCCGTTAAC
GGTGAGTCTGGTTCTCGTAACTGGCGTCTGGGCGTTGAGAGCAAAGCAGGTGGTGCCCTG
TGCGCTAACGGCGCGGTTCGCCAGGGCGACGACGGTGCCGCCCTGGAAGTTATCGAAGTA
CACCGTGTGGGTAACTCTAAAGAACATCTGCCTCTGCCGTCTGAGGCAGGCCCGACCCCA
TGCGCTCCAGCCTCCTTCGAGCGTAAGAACGAACGCAACGCGGAGGCAAAGCGCAAAATG
GCTCTGGCTCGCGAACGCAAGACCGTGAAGACCCTGGGTATTATCATGGGCACTTTCATT
CTGTGCTGGCTGCCTTTCTTCATCGTGGCACTGGTTCTGCCGTTTTGTGAATCCTCTTGC
CACATGCCGACCCTGCTGGGCGCGATCATTAACTGGCTGGGCTATTCTAACAGCCTGCTG
AACCCGGTTATTTATGCGTACTTCAACAAGGACTTCCAGAATGCTTTTAAGAAAATCATC
AAATGCAAGTTTTGCCGCCAGCACCACCATCACCATCACCATCACCATCACTGAAAGCTT
CTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQV
ITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQ
VLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALI
SLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGR
IFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCA
NGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMAL
ARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNP
VIYAYFNKDFQNAFKKIIKCKFCRQHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin
Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50 ug/mL ampicillin, 50 ug/mL kanamycin

Growth Condition: Growth at 37 degrees C in LB supplemented with ampicillin (50 ug/mL) and kanamycin (50 ug/mL)

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_3

Find experimental protocols in GPCR-1

Recommended expression in:

Not Applicable

Vector Name:

pJ4

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin and ampicillin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

3173

Parent Vector:

None

Properties:

mammalian expression

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAATATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCGATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCCGGAGCCGGTGAGCGTGGTTCTCGCGGTATCATCGCAGCGCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

pUC bacterial origin of replication

polypurine tract

polyC

10 base pair poly cysteine tract

3154

3163

primer

M13 forward

M13 forward primer

3022

3037

primer

M13 reverse

M13 reverse primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

2039

selectable marker

AmpR

Ampicillin resistance gene

1380

2039

Gene

Gene Symbol :

HTR1A

Symbol Nomenclature :

HTR1A

SYNONYM :

5-HT-1A; 5-HT1A; 5HT1a; ADRB2RL1; ADRBRL1; G-21; PFMCD

Designation :

5-HT1a receptor|5-hydroxytryptamine receptor 1A|guanine nucleotide-binding regulatory protein-coupled receptor

Full Nomenclature :

5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled

GENEID :

3350

HGNC :

5286

MIM :

109760

Target GenBank:

NP_000515

Reference Sequence Alignment

  

HsCD00292952      GGATCCATGAAGACTATCATTGCTCTGTCCTATATTTTCTGTCTGGTGTTCGCCGATTAC
NM_000524.3       ------------------------------------------------------------
                                                                              

HsCD00292952      AAGGATGACGACGATATGGATGTCCTGTCTCCGGGCCAAGGTAACAACACGACCTCTCCT
NM_000524.3       ---------------ATGGATGTGCTCAGCCCTGGTCAGGGCAACAACACCACATCACCA
                                 ******** ** :  ** ** **.** ******** **.**:**:

HsCD00292952      CCGGCGCCATTCGAAACCGGTGGTAACACCACTGGTATCTCTGACGTAACCGTTTCTTAC
NM_000524.3       CCGGCTCCCTTTGAGACCGGCGGCAACACTACTGGTATCTCCGACGTGACCGTCAGCTAC
                  ***** **.** **.***** ** ***** *********** *****.***** :  ***

HsCD00292952      CAGGTAATCACCTCCCTGCTGCTGGGTACCCTGATTTTCTGTGCTGTTCTGGGCAATGCA
NM_000524.3       CAAGTGATCACCTCTCTGCTGCTGGGCACGCTCATCTTCTGCGCGGTGCTGGGCAATGCG
                  **.**.******** *********** ** ** ** ***** ** ** ***********.

HsCD00292952      TGTGTTGTAGCGGCTATTGCACTGGAACGTAGCCTGCAGAACGTCGCTAATTATCTGATT
NM_000524.3       TGCGTGGTGGCTGCCATCGCCTTGGAGCGCTCCCTGCAGAACGTGGCCAATTATCTTATT
                  ** ** **.** ** ** **. ****.** : ************ ** ******** ***

HsCD00292952      GGCTCTCTGGCAGTAACCGATCTGATGGTATCCGTACTGGTCCTGCCAATGGCAGCGCTG
NM_000524.3       GGCTCTTTGGCGGTCACCGACCTCATGGTGTCGGTGTTGGTGCTGCCCATGGCCGCGCTG
                  ****** ****.**.***** ** *****.** **. **** *****.*****.******

HsCD00292952      TATCAGGTTCTGAACAAGTGGACGCTGGGCCAAGTAACTTGTGATCTGTTCATTGCGCTG
NM_000524.3       TATCAGGTGCTCAACAAGTGGACACTGGGCCAGGTAACCTGCGACCTGTTCATCGCCCTC
                  ******** ** ***********.********.***** ** ** ******** ** ** 

HsCD00292952      GACGTTCTGTGCTGCACCAGCAGCATCCTGCACCTGTGCGCTATCGCTCTGGACCGCTAC
NM_000524.3       GACGTGCTGTGCTGCACCTCATCCATCTTGCACCTGTGCGCCATCGCGCTGGACAGGTAC
                  ***** ************: .: **** ************* ***** ******.* ***

HsCD00292952      TGGGCCATCACCGACCCGATCGATTACGTGAACAAACGTACTCCGCGCCGTGCTGCCGCC
NM_000524.3       TGGGCCATCACGGACCCCATCGACTACGTGAACAAGAGGACGCCCCGGCGCGCCGCTGCG
                  *********** ***** ***** ***********..* ** ** ** ** ** ** ** 

HsCD00292952      CTGATCTCTCTGACGTGGCTGATCGGTTTCCTGATCAGCATCCCTCCGATGCTGGGCTGG
NM_000524.3       CTCATCTCGCTCACTTGGCTTATTGGCTTCCTCATCTCTATCCCGCCCATGCTGGGCTGG
                  ** ***** ** ** ***** ** ** ***** ***:  ***** ** ************

HsCD00292952      CGTACCCCTGAGGATCGTTCTGACCCTGACGCTTGCACGATTAGCAAGGACCACGGCTAC
NM_000524.3       CGCACCCCGGAAGACCGCTCGGACCCCGACGCATGCACCATTAGCAAGGATCATGGCTAC
                  ** ***** **.** ** ** ***** *****:***** *********** ** ******

HsCD00292952      ACCATCTACTCCACTTTCGGTGCTTTCTATATCCCTCTGCTGCTGATGCTGGTACTGTAC
NM_000524.3       ACTATCTATTCCACCTTTGGAGCTTTCTACATCCCGCTGCTGCTCATGCTGGTTCTCTAT
                  ** ***** ***** ** **:******** ***** ******** ********:** ** 

HsCD00292952      GGCCGTATCTTCCGTGCTGCCCGCTTTCGCATCCGCAAAACCGTGAAGAAAGTCGAGAAA
NM_000524.3       GGGCGCATATTCCGAGCTGCGCGCTTCCGCATCCGCAAGACGGTCAAAAAGGTGGAGAAG
                  ** ** **.*****:***** ***** ***********.** ** **.**.** *****.

HsCD00292952      ACCGGTGCGGATACCCGTCATGGTGCGTCTCCGGCACCGCAACCGAAGAAATCCGTTAAC
NM_000524.3       ACCGGAGCGGACACCCGCCATGGAGCATCTCCCGCCCCGCAGCCCAAGAAGAGTGTGAAT
                  *****:***** ***** *****:**.***** **.*****.** *****.:  ** ** 

HsCD00292952      GGTGAGTCTGGTTCTCGTAACTGGCGTCTGGGCGTTGAGAGCAAAGCAGGTGGTGCCCTG
NM_000524.3       GGAGAGTCGGGGAGCAGGAACTGGAGGCTGGGCGTGGAGAGCAAGGCTGGGGGTGCTCTG
                  **:***** ** :  .* ******.* ******** ********.**:** ***** ***

HsCD00292952      TGCGCTAACGGCGCGGTTCGCCAGGGCGACGACGGTGCCGCCCTGGAAGTTATCGAAGTA
NM_000524.3       TGCGCCAATGGCGCGGTGAGGCAAGGTGACGATGGCGCCGCCCTGGAGGTGATCGAGGTG
                  ***** ** ******** .* **.** ***** ** ***********.** *****.**.

HsCD00292952      CACCGTGTGGGTAACTCTAAAGAACATCTGCCTCTGCCGTCTGAGGCAGGCCCGACCCCA
NM_000524.3       CACCGAGTGGGCAACTCCAAAGAGCACTTGCCTCTGCCCAGCGAGGCTGGTCCTACCCCT
                  *****:***** ***** *****.**  ********** :  *****:** ** *****:

HsCD00292952      TGCGCTCCAGCCTCCTTCGAGCGTAAGAACGAACGCAACGCGGAGGCAAAGCGCAAAATG
NM_000524.3       TGTGCCCCCGCCTCTTTCGAGAGGAAAAATGAGCGCAACGCCGAGGCGAAGCGCAAGATG
                  ** ** **.***** ******.* **.** **.******** *****.********.***

HsCD00292952      GCTCTGGCTCGCGAACGCAAGACCGTGAAGACCCTGGGTATTATCATGGGCACTTTCATT
NM_000524.3       GCCCTGGCCCGAGAGAGGAAGACAGTGAAGACGCTGGGCATCATCATGGGCACCTTCATC
                  ** ***** **.**..* *****.******** ***** ** *********** ***** 

HsCD00292952      CTGTGCTGGCTGCCTTTCTTCATCGTGGCACTGGTTCTGCCGTTTTGTGAATCCTCTTGC
NM_000524.3       CTCTGCTGGCTGCCCTTCTTCATCGTGGCTCTTGTTCTGCCCTTCTGCGAGAGCAGCTGC
                  ** *********** **************:** ******** ** ** **.: *:  ***

HsCD00292952      CACATGCCGACCCTGCTGGGCGCGATCATTAACTGGCTGGGCTATTCTAACAGCCTGCTG
NM_000524.3       CACATGCCCACCCTGTTGGGCGCCATAATCAATTGGCTGGGCTACTCCAACTCTCTGCTT
                  ******** ****** ******* **.** ** *********** ** ***:  ***** 

HsCD00292952      AACCCGGTTATTTATGCGTACTTCAACAAGGACTTCCAGAATGCTTTTAAGAAAATCATC
NM_000524.3       AACCCCGTCATTTACGCATACTTCAACAAGGACTTTCAAAACGCGTTTAAGAAGATCATT
                  ***** ** ***** **.***************** **.** ** ********.***** 

HsCD00292952      AAATGCAAGTTTTGCCGCCAGCACCACCATCACCATCACCATCACCATCACTGAAAGCTT
NM_000524.3       AAGTGTAAGTTCTGCCGCCAGTGA------------------------------------
                  **.** ***** ********* ..                                    

HsCD00292952      CTCGAG
NM_000524.3       ------

Pathways

Panther :

5HT1 type receptor mediated signaling pathway

Panther :

Heterotrimeric G-protein signaling pathway-Gi alpha and Gs alpha mediated pathway

Reactome :

Amine ligand-binding receptors

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (i) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Serotonin receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class A Rhodopsin-like

WikiPathway :

Monoamine GPCRs

WikiPathway :

Serotonin HTR1 Group and FOS Pathway

WikiPathway :

SIDS Susceptibility Pathways

Protein

Structural Annotation Wiki :

TOPSAN

UniProt :

P08908

UniProt :

Q5ZGX3

HPRD :

00190

Clone

Cloning Information :

485316

HIP Master Clone ID :

401873

Original Clone ID :

ATCG3D_15

Original Clone ID :

GPCR-1

Experimental

TargetTrack - Experimental data :

ATCG3D_3

TargetTrack - Experimental data :

GPCR-1

Organism

Species Specific ID:

3350

Genome

Chromosome :

Map Location :

5q11.2-q13

Reagents

Labome :

HTR1A-antibody