DNASU Plasmid | CRHR1 (Homo sapiens) in pJ2 (Cloning)

Explanation of Terms

Gene:

Gene Symbol: CRHR1

Gene Name: corticotropin releasing hormone receptor 1

Original Clone ID:

ATCG3D_9

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pJ2

Format:

CLOSED

Source:

Accelerated Technologies Center for Gene to 3D Structure

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. Codon Usage Engineered in Gene Composer for Expression in E. coli and BV. Supplied in the Vendor's Vector.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
Accelerated Technologies Center for Gene to 3D Structure

HsCD00292955

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1434nts

Open reading frame: 7 to 1440

Reference Sequence Alignment

GGATCCATGAAGACGATCATTGCACTGAGCTACATCTTCTGTCTGGTGTTTGCCGACTAT
AAGGATGACGATGACATGGGTGGCCACCCTCAGCTGCGCCTGGTTAAGGCACTGCTCCTG
CTCGGACTGAACCCAGTGTCCGCTAGTCTCCAGGACCAGCATTGTGAGAGCCTGAGCCTT
GCTTCTAACATCTCAGGGCTCCAGTGTAATGCTTCTGTGGACCTCATCGGTACTTGTTGG
CCGCGTTCACCTGCTGGCCAGCTGGTTGTGCGTCCATGCCCCGCCTTCTTTTACGGCGTG
CGCTATAACACGACCAACAACGGGTACCGTGAGTGTCTCGCAAATGGCTCCTGGGCCGCA
CGCGTGAACTACAGCGAGTGCCAGGAAATCCTTAACGAGGAGAAGAAATCCAAGGTTCAC
TACCACGTTGCTGTGATCATTAACTATCTGGGCCACTGTATCTCACTTGTGGCCCTTCTT
GTCGCCTTCGTGCTGTTCCTCCGCCTGCGCCCAGGGTGTACCCACTGGGGTGACCAAGCT
GACGGCGCTCTGGAGGTTGGTGCCCCCTGGAGTGGCGCACCATTCCAGGTTCGTCGCTCA
ATCCGTTGTCTGCGTAACATCATTCACTGGAATCTGATCAGCGCCTTTATCCTCCGCAAC
GCCACCTGGTTTGTGGTGCAGCTGACCATGTCACCTGAGGTTCATCAGTCTAATGTGGGA
TGGTGTCGCCTGGTGACAGCCGCATATAACTACTTCCATGTGACTAACTTCTTTTGGATG
TTCGGCGAGGGTTGCTACCTGCACACCGCCATTGTGCTGACTTACTCCACAGACCGTCTG
CGTAAGTGGATGTTTATTTGCATTGGCTGGGGAGTGCCTTTCCCCATCATTGTTGCCTGG
GCTATTGGTAAGCTCTATTACGACAACGAGAAGTGCTGGTTCGGGAAGCGCCCTGGCGTC
TACACCGATTACATCTACCAGGGCCCCATGATCCTGGTGCTCCTTATCAACTTTATCTTC
CTGTTTAACATCGTTCGTATCCTGATGACAAAGCTGCGTGCAAGTACCACTTCCGAAACA
ATTCAGTACCGTAAAGCCGTGAAGGCTACGCTGGTCCTGCTGCCTCTGCTGGGTATTACC
TATATGCTGTTCTTCGTGAATCCAGGGGAGGACGAGGTCTCCCGTGTGGTTTTCATCTAC
TTCAACTCTTTTCTGGAGTCCTTTCAAGGCTTCTTCGTGTCTGTGTTCTACTGTTTCCTG
AACAGTGAAGTTCGTTCCGCTATTCGTAAGCGCTGGCATCGTTGGCAGGACAAACACTCC
ATCCGTGCTCGCGTGGCCCGTGCAATGTCCATTCCGACTTCTCCAACGCGCGTGTCCTTC
CACTCCATTAAGCAGTCCACTGCCGTTCACCACCATCACCATCACCATCACCATCACTGA
AAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MKTIIALSYIFCLVFADYKDDDDMGGHPQLRLVKALLLLGLNPVSASLQDQHCESLSLAS
NISGLQCNASVDLIGTCWPRSPAGQLVVRPCPAFFYGVRYNTTNNGYRECLANGSWAARV
NYSECQEILNEEKKSKVHYHVAVIINYLGHCISLVALLVAFVLFLRLRPGCTHWGDQADG
ALEVGAPWSGAPFQVRRSIRCLRNIIHWNLISAFILRNATWFVVQLTMSPEVHQSNVGWC
RLVTAAYNYFHVTNFFWMFGEGCYLHTAIVLTYSTDRLRKWMFICIGWGVPFPIIVAWAI
GKLYYDNEKCWFGKRPGVYTDYIYQGPMILVLLINFIFLFNIVRILMTKLRASTTSETIQ
YRKAVKATLVLLPLLGITYMLFFVNPGEDEVSRVVFIYFNSFLESFQGFFVSVFYCFLNS
EVRSAIRKRWHRWQDKHSIRARVARAMSIPTSPTRVSFHSIKQSTAVHHHHHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: kanamycin

Growth Condition: Growth in 30 ug/ml of kanamycin in LB at 37 degrees

Comments: None

Protein Expression Results:

ProteinExpressed: Not_Applicable

ProteinPurified: Not_Applicable

SolubleProtein: Not_Applicable

Find experimental protocols in ATCG3D_9

Recommended expression in:

Not Applicable

Vector Name:

pJ2

Synonyms:

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Cloning vector; kanamycin resistance in bacteria; restriction enzyme cloning.

Comments:

Supplied by DNA2.0

Size (bp):

2213

Parent Vector:

None

Properties:

TOPO Cloning

Author Name:

DNA2.0
Accelerated Technologies Center for Gene to 3D Structure

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGTACAAGATAAAATAATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCACTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCGTTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCACTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGCCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTACGCGCGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGCCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGGCGAGAGTAGGGAACTGCCAGGCATCAAACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTCTGTGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACACATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCCCCCCCCCACCCAGCTTT

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pUC ori

Bacterial origin of replication

primer

M13 forward

M13 forward sequencing primer

2062

2077

primer

M13 reverse

M13 reverse sequencing primer

114

131

selectable marker

KanR

Kanamycin resistance gene

268

1050

Gene

Gene Symbol :

CRHR1

Symbol Nomenclature :

CRHR1

SYNONYM :

CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRH-R1h; CRHR; CRHR1L; CRHR1f

Designation :

CRH receptor 1 variant B|corticotropin releasing hormone receptor variant 1e|corticotropin releasing hormone receptor variant 1g|corticotropin-releasing factor receptor 1|corticotropin-releasing factor type 1 receptor|corticotropin-releasing hormone receptor 1|seven transmembrane helix receptor

Full Nomenclature :

corticotropin releasing hormone receptor 1

GENEID :

1394

HGNC :

2357

MIM :

122561

Vega :

OTTHUMG00000178271

Target GenBank:

P34998

Reference Sequence Alignment

  

HsCD00292955        GGATCCATGAAGACGATCATTGCACTGAGCTACATCTTCTGTCTGGTGTTTGCCGACTAT
NM_001145146.1      ------------------------------------------------------------
                                                                                

HsCD00292955        AAGGATGACGATGACATGGGTGGCCACCCTCAGCTGCGCCTGGTTAAGGCACTGCTCCTG
NM_001145146.1      ---------------ATGGGAGGGCACCCGCAGCTCCGTCTCGTCAAGGCCCTTCTCCTT
                                   *****:** ***** ***** ** ** ** *****.** ***** 

HsCD00292955        CTCGGACTGAACCCAGTGTCCGCTAGTCTCCAGGACCAGCATTGTGAGAGCCTGAGCCTT
NM_001145146.1      CTGGGGCTGAACCCCGTCTCTGCCTCCCTCCAGGACCAGCACTGCGAGAGCCTGTCCCTG
                    ** **.********.** ** ** :  ************** ** *********: *** 

HsCD00292955        GCTTCTAACATCTCAGGGCTCCAGTGTAATGCTTCTGTGGACCTCATCGGTACTTGTTGG
NM_001145146.1      GCCAGCAACATCTCAGGACTGCAGTGCAACGCATCCGTGGACCTCATTGGCACCTGCTGG
                    ** :  ***********.** ***** ** **:** *********** ** ** ** ***

HsCD00292955        CCGCGTTCACCTGCTGGCCAGCTGGTTGTGCGTCCATGCCCCGCCTTCTTTTACGGCGTG
NM_001145146.1      CCCCGCAGCCCTGCGGGGCAGCTAGTGGTTCGGCCCTGCCCTGCCTTTTTCTATGGTGTC
                    ** ** : .***** ** *****.** ** ** **.***** ***** ** ** ** ** 

HsCD00292955        CGCTATAACACGACCAACAACGGGTACCGTGAGTGTCTCGCAAATGGCTCCTGGGCCGCA
NM_001145146.1      CGCTACAATACCACAAACAATGGCTACCGGGAGTGCCTGGCCAATGGCAGCTGGGCCGCC
                    ***** ** ** **.***** ** ***** ***** ** **.******: *********.

HsCD00292955        CGCGTGAACTACAGCGAGTGCCAGGAAATCCTTAACGAGGAGAAGAAATCCAAGGTTCAC
NM_001145146.1      CGCGTGAATTACTCCGAGTGCCAGGAGATCCTCAATGAGGAGAAAAAAAGCAAGGTGCAC
                    ******** ***: ************.***** ** ********.***: ****** ***

HsCD00292955        TACCACGTTGCTGTGATCATTAACTATCTGGGCCACTGTATCTCACTTGTGGCCCTTCTT
NM_001145146.1      TACCATGTCGCAGTCATCATCAACTACCTGGGCCACTGTATCTCCCTGGTGGCCCTCCTG
                    ***** ** **:** ***** ***** *****************.** ******** ** 

HsCD00292955        GTCGCCTTCGTGCTGTTCCTCCGCCTGCGCCCAGGGTGTACCCACTGGGGTGACCAAGCT
NM_001145146.1      GTGGCCTTTGTCCTCTTTCTGCGGCTCAGGCCAGGCTGCACCCATTGGGGTGACCAGGCA
                    ** ***** ** ** ** ** ** ** .* ***** ** ***** ***********.**:

HsCD00292955        GACGGCGCTCTGGAGGTTGGTGCCCCCTGGAGTGGCGCACCATTCCAGGTTCGTCGCTCA
NM_001145146.1      GATGGAGCCCTGGAGGTGGGGGCTCCATGGAGTGGTGCCCCATTTCAGGTTCGAAGGAGC
                    ** **.** ******** ** ** **.******** **.***** ********:.* : .

HsCD00292955        ATCCGTTGTCTGCGTAACATCATTCACTGGAATCTGATCAGCGCCTTTATCCTCCGCAAC
NM_001145146.1      ATCCGGTGCCTGCGAAACATCATCCACTGGAACCTCATCTCCGCCTTCATCCTGCGCAAC
                    ***** ** *****:******** ******** ** ***: ****** ***** ******

HsCD00292955        GCCACCTGGTTTGTGGTGCAGCTGACCATGTCACCTGAGGTTCATCAGTCTAATGTGGGA
NM_001145146.1      GCCACCTGGTTCGTGGTCCAGCTAACCATGAGCCCCGAGGTCCACCAGAGCAACGTGGGC
                    *********** ***** *****.******: .** ***** ** ***:  ** *****.

HsCD00292955        TGGTGTCGCCTGGTGACAGCCGCATATAACTACTTCCATGTGACTAACTTCTTTTGGATG
NM_001145146.1      TGGTGCAGGTTGGTGACAGCCGCCTACAACTACTTCCATGTGACCAACTTCTTCTGGATG
                    ***** .*  *************.** ***************** ******** ******

HsCD00292955        TTCGGCGAGGGTTGCTACCTGCACACCGCCATTGTGCTGACTTACTCCACAGACCGTCTG
NM_001145146.1      TTCGGCGAGGGCTGCTACCTGCACACAGCCATCGTGCTCACCTACTCCACTGACCGGCTG
                    *********** **************.***** ***** ** ********:***** ***

HsCD00292955        CGTAAGTGGATGTTTATTTGCATTGGCTGGGGAGTGCCTTTCCCCATCATTGTTGCCTGG
NM_001145146.1      CGCAAATGGATGTTCATCTGCATTGGCTGGGGTGTGCCCTTCCCCATCATTGTGGCCTGG
                    ** **.******** ** **************:***** ************** ******

HsCD00292955        GCTATTGGTAAGCTCTATTACGACAACGAGAAGTGCTGGTTCGGGAAGCGCCCTGGCGTC
NM_001145146.1      GCCATTGGGAAGCTGTACTACGACAATGAGAAGTGCTGGTTTGGCAAAAGGCCTGGGGTG
                    ** ***** ***** ** ******** ************** ** **..* ***** ** 

HsCD00292955        TACACCGATTACATCTACCAGGGCCCCATGATCCTGGTGCTCCTTATCAACTTTATCTTC
NM_001145146.1      TACACCGACTACATCTACCAGGGCCCCATGATCCTGGTCCTGCTGATCAATTTCATCTTC
                    ******** ***************************** ** ** ***** ** ******

HsCD00292955        CTGTTTAACATCGTTCGTATCCTGATGACAAAGCTGCGTGCAAGTACCACTTCCGAAACA
NM_001145146.1      CTTTTCAACATCGTCCGCATCCTCATGACCAAGCTCCGGGCATCCACCACGTCTGAGACC
                    ** ** ******** ** ***** *****.***** ** ***:  ***** ** **.**.

HsCD00292955        ATTCAGTACCGTAAAGCCGTGAAGGCTACGCTGGTCCTGCTGCCTCTGCTGGGTATTACC
NM_001145146.1      ATTCAGTACAGGAAGGCTGTGAAAGCCACTCTGGTGCTGCTGCCCCTCCTGGGCATCACC
                    *********.* **.** *****.** ** ***** ******** ** ***** ** ***

HsCD00292955        TATATGCTGTTCTTCGTGAATCCAGGGGAGGACGAGGTCTCCCGTGTGGTTTTCATCTAC
NM_001145146.1      TACATGCTGTTCTTCGTCAATCCCGGGGAGGATGAGGTCTCCCGGGTCGTCTTCATCTAC
                    ** ************** *****.******** *********** ** ** *********

HsCD00292955        TTCAACTCTTTTCTGGAGTCCTTTCAAGGCTTCTTCGTGTCTGTGTTCTACTGTTTCCTG
NM_001145146.1      TTCAACTCCTTCCTGGAATCCTTCCAGGGCTTCTTTGTGTCTGTGTTCTACTGTTTCCTC
                    ******** ** *****.***** **.******** *********************** 

HsCD00292955        AACAGTGAAGTTCGTTCCGCTATTCGTAAGCGCTGGCATCGTTGGCAGGACAAACACTCC
NM_001145146.1      AATAGTGAGGTCCGTTCTGCCATCCGGAAGAGGTGGCACCGGTGGCAGGACAAGCACTCG
                    ** *****.** ***** ** ** ** ***.* ***** ** ***********.***** 

HsCD00292955        ATCCGTGCTCGCGTGGCCCGTGCAATGTCCATTCCGACTTCTCCAACGCGCGTGTCCTTC
NM_001145146.1      ATCCGTGCCCGAGTGGCCCGTGCCATGTCCATCCCCACCTCCCCAACCCGTGTCAGCTTT
                    ******** **.***********.******** ** ** ** ***** ** ** : *** 

HsCD00292955        CACTCCATTAAGCAGTCCACTGCCGTTCACCACCATCACCATCACCATCACCATCACTGA
NM_001145146.1      CACAGCATCAAGCAGTCCACAGCAGTCTGA------------------------------
                    ***: *** ***********:**.**  ..                              

HsCD00292955        AAGCTTCTCGAG
NM_001145146.1      ------------

Pathways

Panther :

Cortocotropin releasing factor receptor signaling pathway

Reactome :

Class B/2 (Secretin family receptors)

Reactome :

G alpha (s) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class B Secretin-like

WikiPathway :

Myometrial Relaxation and Contraction Pathways

Protein

SMART domain :

HormR : Domain present in hormone receptors

Structural Annotation Wiki :

TOPSAN

UniProt :

P34998

HPRD :

08830

Clone

Cloning Information :

485319

HIP Master Clone ID :

401876

Original Clone ID :

ATCG3D_9

Experimental

TargetTrack - Experimental data :

ATCG3D_9

Organism

Species Specific ID:

1394

Genome

Chromosome :

Map Location :

17q12-q22

Ensembl :

ENSG00000120088

Reagents

Labome :

CRHR1-antibody