DNASU Plasmid | ADORA2A (H. sapiens )<br>In pET28a (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: ADORA2A

Gene Name: adenosine A2a receptor

Original Clone ID:

ATCG3D_48

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET28a

Format:

CLOSED

Source:

GPCR Network

Description:

G-protein coupled receptor

Comments:

Clone insert was fully verified. The insert sequence displayed below was provided by clone submitter. ATCG3D-29 Cloned into our pET28a Expression Vector.

Mutation / Discrepancy:

No / No

Publications:

PMID: 18832607
Title: The 2.6 angstrom crystal structure of a human A2A adenosine receptor bound to an antagonist.
PMID: 21393508
Title: Structure of an agonist-bound human A2A adenosine receptor.

Authors:

PSI
PSI:Biology
Accelerated Technologies Center for Gene to 3D Structure
GPCR Network

HsCD00292958

Price:

AVAILABLE

No restriction
Special MTA: PSI/T7

Insert Sequence
Protein Sequence

Insert sequence: 1236nts

Open reading frame: 76 to 1311

Reference Sequence Alignment

GGATCCATGAAGACAATTATCGCTTTGAGTTACATATTCTGTTTGGTTTTTGCGGACTAC
AAGGATGACGACGATATGCCGATCATGGGATCGTCAGTTTACATCACCGTTGAACTTGCT
ATAGCTGTACTCGCTATCCTTGGTAACGTACTCGTTTGCTGGGCGGTGTGGCTCAACTCG
AACCTTCAGAACGTCACAAATTACTTCGTAGTCTCTCTGGCGGCTGCGGACATTGCGGTA
GGCGTTCTCGCCATCCCGTTCGCCATCACTATCTCTACCGGTTTCTGTGCCGCTTGCCAC
GGCTGCCTCTTTATTGCTTGCTTCGTTTTGGTACTTACCCAATCTTCTATCTTCTCGCTC
TTGGCTATCGCGATCGACAGATACATAGCTATCCGTATCCCTCTGAGGTACAATGGACTG
GTGACAGGCACACGTGCTAAGGGCATCATCGCTATCTGCTGGGTATTGTCATTCGCTATC
GGACTGACTCCAATGCTGGGCTGGAACAACTGTGGACAGCCTAAGGAAGGAAAGAACCAC
TCTCAAGGATGTGGCGAAGGTCAAGTCGCGTGCCTCTTTGAAGACGTAGTGCCGATGAAC
TATATGGTCTACTTCAACTTCTTTGCTTGCGTCCTGGTGCCGCTGCTCTTGATGCTTGGT
GTATACCTCCGTATCTTCCTTGCCGCTAGACGTCAGCTTAAACAGATGGAGTCTCAACCT
CTTCCTGGAGAGAGAGCGAGGTCAACTTTGCAGAAGGAGGTACATGCCGCCAAGTCATTG
GCTATCATCGTCGGTCTTTTTGCGCTGTGCTGGCTGCCCCTCCACATCATCAACTGTTTT
ACCTTCTTCTGTCCCGACTGTTCACACGCACCTCTTTGGCTGATGTACCTGGCGATCGTT
CTCAGCCACACCAATTCGGTCGTAAATCCGTTTATCTATGCTTACCGTATTCGTGAATTT
CGCCAGACGTTTCGCAAGATCATCAGGTCCCATGTCCTCAGACAGCAAGAACCTTTCAAG
GCTGCCGGCACCTCCGCCAGGGTCTTGGCGGCGCACGGAAGCGACGGTGAGCAAGTGTCT
TTGCGCCTTAACGGACACCCTCCCGGTGTCTGGGCGAACGGCTCTGCCCCACACCCCGAG
AGACGCCCCAATGGTTACGCTCTGGGACTTGTGTCTGGCGGATCAGCTCAGGAAAGCCAA
GGCAACACTGGTTTGCCTGACGTCGAACTGCTGTCGCACGAGTTGAAGGGCGTTTGCCCG
GAGCCGCCTGGCTTGGACGACCCCTTGGCACAGGACGGTGCTGGCGTTTCGCACCACCAT
CACCATCACCATCACCATCACTGAAAGCTTCTCGAG

Protein sequence predicted based on nucleotide sequence

MPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYFVVSLAAADIAVGVLAI
PFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVTGTR
AKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEGQVACLFEDVVPMNYMVYF
NFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPLPGERARSTLQKEVHAAKSLAIIVG
LFALCWLPLHIINCFTFFCPDCSHAPLWLMYLAIVLSHTNSVVNPFIYAYRIREFRQTFR
KIIRSHVLRQQEPFKAAGTSARVLAAHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNG
YALGLVSGGSAQESQGNTGLPDVELLSHELKGVCPEPPGLDDPLAQDGAGVS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in ATCG3D_5

Find experimental protocols in GPCR-6

Recommended expression in:

Not Applicable

Vector Name:

pET28a

Synonyms:

pET-28a, pET28

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial expression vector with T7lac promoter, adds N-terminal His tag, thrombin cleavage site, internal T7 epitope tag, C-terminal His tag; kanamycin resistance; restriction enzyme cloning.

Comments:

None

Size (bp):

5369

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Midwest Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGCGGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (XhoI, NotI, EagI, HindIII, SalI, SacI, EcoRI, BamHI, NheI, NdeI, NcoI)

158

203

bacterial origin

f1 origin

4903

5358

bacterial origin

ori

bacterial origin of replication

3270

3889

promoter

T7 promoter

368

386

repressor protein gene

LacI

lacI coding sequence (lacI cds)

896

1852

selectable marker

KanR

kanamycin resistance

3995

4807

tag

His

6xHis tag

140

157

trxn regulatory element

lac operator

347

363

trxn termination sequence

T7 term

T7 terminator

Gene

Gene Symbol :

ADORA2A

Symbol Nomenclature :

ADORA2A

SYNONYM :

A2aR; ADORA2; RDC8

Designation :

adenosine receptor A2a|adenosine receptor subtype A2a

Full Nomenclature :

adenosine A2a receptor

GENEID :

135

HGNC :

263

MIM :

102776

Vega :

OTTHUMG00000150761

Target GenBank:

NP_000666

Reference Sequence Alignment

  

HsCD00292958        GGATCCATGAAGACAATTATCGCTTTGAGTTACATATTCTGTTTGGTTTTTGCGGACTAC
NM_001278500.1      ------------------------------------------------------------
NM_000675.5         ------------------------------------------------------------
NM_001278498.1      ------------------------------------------------------------
NM_001278497.1      ------------------------------------------------------------
NM_001278499.1      ------------------------------------------------------------
                                                                                

HsCD00292958        AAGGATGACGACGATATGCCGATCATGGGATCGTCAGTTTACATCACCGTTGAACTTGCT
NM_001278500.1      ---------------ATGCCCATCATGGGCTCCTCGGTGTACATCACGGTGGAGCTGGCC
NM_000675.5         ---------------ATGCCCATCATGGGCTCCTCGGTGTACATCACGGTGGAGCTGGCC
NM_001278498.1      ---------------ATGCCCATCATGGGCTCCTCGGTGTACATCACGGTGGAGCTGGCC
NM_001278497.1      ---------------ATGCCCATCATGGGCTCCTCGGTGTACATCACGGTGGAGCTGGCC
NM_001278499.1      ---------------ATGCCCATCATGGGCTCCTCGGTGTACATCACGGTGGAGCTGGCC
                                   ***** ********.** **.** ******** ** **.** ** 

HsCD00292958        ATAGCTGTACTCGCTATCCTTGGTAACGTACTCGTTTGCTGGGCGGTGTGGCTCAACTCG
NM_001278500.1      ATTGCTGTGCTGGCCATCCTGGGCAATGTGCTGGTGTGCTGGGCCGTGTGGCTCAACAGC
NM_000675.5         ATTGCTGTGCTGGCCATCCTGGGCAATGTGCTGGTGTGCTGGGCCGTGTGGCTCAACAGC
NM_001278498.1      ATTGCTGTGCTGGCCATCCTGGGCAATGTGCTGGTGTGCTGGGCCGTGTGGCTCAACAGC
NM_001278497.1      ATTGCTGTGCTGGCCATCCTGGGCAATGTGCTGGTGTGCTGGGCCGTGTGGCTCAACAGC
NM_001278499.1      ATTGCTGTGCTGGCCATCCTGGGCAATGTGCTGGTGTGCTGGGCCGTGTGGCTCAACAGC
                    **:*****.** ** ***** ** ** **.** ** ******** ************:  

HsCD00292958        AACCTTCAGAACGTCACAAATTACTTCGTAGTCTCTCTGGCGGCTGCGGACATTGCGGTA
NM_001278500.1      AACCTGCAGAACGTCACCAACTACTTTGTGGTGTCACTGGCGGCGGCCGACATCGCAGTG
NM_000675.5         AACCTGCAGAACGTCACCAACTACTTTGTGGTGTCACTGGCGGCGGCCGACATCGCAGTG
NM_001278498.1      AACCTGCAGAACGTCACCAACTACTTTGTGGTGTCACTGGCGGCGGCCGACATCGCAGTG
NM_001278497.1      AACCTGCAGAACGTCACCAACTACTTTGTGGTGTCACTGGCGGCGGCCGACATCGCAGTG
NM_001278499.1      AACCTGCAGAACGTCACCAACTACTTTGTGGTGTCACTGGCGGCGGCCGACATCGCAGTG
                    ***** ***********.** ***** **.** **:******** ** ***** **.**.

HsCD00292958        GGCGTTCTCGCCATCCCGTTCGCCATCACTATCTCTACCGGTTTCTGTGCCGCTTGCCAC
NM_001278500.1      GGTGTGCTCGCCATCCCCTTTGCCATCACCATCAGCACCGGGTTCTGCGCTGCCTGCCAC
NM_000675.5         GGTGTGCTCGCCATCCCCTTTGCCATCACCATCAGCACCGGGTTCTGCGCTGCCTGCCAC
NM_001278498.1      GGTGTGCTCGCCATCCCCTTTGCCATCACCATCAGCACCGGGTTCTGCGCTGCCTGCCAC
NM_001278497.1      GGTGTGCTCGCCATCCCCTTTGCCATCACCATCAGCACCGGGTTCTGCGCTGCCTGCCAC
NM_001278499.1      GGTGTGCTCGCCATCCCCTTTGCCATCACCATCAGCACCGGGTTCTGCGCTGCCTGCCAC
                    ** ** *********** ** ******** ***:  ***** ***** ** ** ******

HsCD00292958        GGCTGCCTCTTTATTGCTTGCTTCGTTTTGGTACTTACCCAATCTTCTATCTTCTCGCTC
NM_001278500.1      GGCTGCCTCTTCATTGCCTGCTTCGTCCTGGTCCTCACGCAGAGCTCCATCTTCAGTCTC
NM_000675.5         GGCTGCCTCTTCATTGCCTGCTTCGTCCTGGTCCTCACGCAGAGCTCCATCTTCAGTCTC
NM_001278498.1      GGCTGCCTCTTCATTGCCTGCTTCGTCCTGGTCCTCACGCAGAGCTCCATCTTCAGTCTC
NM_001278497.1      GGCTGCCTCTTCATTGCCTGCTTCGTCCTGGTCCTCACGCAGAGCTCCATCTTCAGTCTC
NM_001278499.1      GGCTGCCTCTTCATTGCCTGCTTCGTCCTGGTCCTCACGCAGAGCTCCATCTTCAGTCTC
                    *********** ***** ********  ****.** ** **.:  ** ******:  ***

HsCD00292958        TTGGCTATCGCGATCGACAGATACATAGCTATCCGTATCCCTCTGAGGTACAATGGACTG
NM_001278500.1      CTGGCCATCGCCATTGACCGCTACATTGCCATCCGCATCCCGCTCCGGTACAATGGCTTG
NM_000675.5         CTGGCCATCGCCATTGACCGCTACATTGCCATCCGCATCCCGCTCCGGTACAATGGCTTG
NM_001278498.1      CTGGCCATCGCCATTGACCGCTACATTGCCATCCGCATCCCGCTCCGGTACAATGGCTTG
NM_001278497.1      CTGGCCATCGCCATTGACCGCTACATTGCCATCCGCATCCCGCTCCGGTACAATGGCTTG
NM_001278499.1      CTGGCCATCGCCATTGACCGCTACATTGCCATCCGCATCCCGCTCCGGTACAATGGCTTG
                     **** ***** ** ***.*.*****:** ***** ***** ** .**********. **

HsCD00292958        GTGACAGGCACACGTGCTAAGGGCATCATCGCTATCTGCTGGGTATTGTCATTCGCTATC
NM_001278500.1      GTGACCGGCACGAGGGCTAAGGGCATCATTGCCATCTGCTGGGTGCTGTCGTTTGCCATC
NM_000675.5         GTGACCGGCACGAGGGCTAAGGGCATCATTGCCATCTGCTGGGTGCTGTCGTTTGCCATC
NM_001278498.1      GTGACCGGCACGAGGGCTAAGGGCATCATTGCCATCTGCTGGGTGCTGTCGTTTGCCATC
NM_001278497.1      GTGACCGGCACGAGGGCTAAGGGCATCATTGCCATCTGCTGGGTGCTGTCGTTTGCCATC
NM_001278499.1      GTGACCGGCACGAGGGCTAAGGGCATCATTGCCATCTGCTGGGTGCTGTCGTTTGCCATC
                    *****.*****..* ************** ** ***********. ****.** ** ***

HsCD00292958        GGACTGACTCCAATGCTGGGCTGGAACAACTGTGGACAGCCTAAGGAAGGAAAGAACCAC
NM_001278500.1      GGCCTGACTCCCATGCTAGGTTGGAACAACTGCGGTCAGCCAAAGGAGGGCAAGAACCAC
NM_000675.5         GGCCTGACTCCCATGCTAGGTTGGAACAACTGCGGTCAGCCAAAGGAGGGCAAGAACCAC
NM_001278498.1      GGCCTGACTCCCATGCTAGGTTGGAACAACTGCGGTCAGCCAAAGGAGGGCAAGAACCAC
NM_001278497.1      GGCCTGACTCCCATGCTAGGTTGGAACAACTGCGGTCAGCCAAAGGAGGGCAAGAACCAC
NM_001278499.1      GGCCTGACTCCCATGCTAGGTTGGAACAACTGCGGTCAGCCAAAGGAGGGCAAGAACCAC
                    **.********.*****.** *********** **:*****:*****.**.*********

HsCD00292958        TCTCAAGGATGTGGCGAAGGTCAAGTCGCGTGCCTCTTTGAAGACGTAGTGCCGATGAAC
NM_001278500.1      TCCCAGGGCTGCGGGGAGGGCCAAGTGGCCTGTCTCTTTGAGGATGTGGTCCCCATGAAC
NM_000675.5         TCCCAGGGCTGCGGGGAGGGCCAAGTGGCCTGTCTCTTTGAGGATGTGGTCCCCATGAAC
NM_001278498.1      TCCCAGGGCTGCGGGGAGGGCCAAGTGGCCTGTCTCTTTGAGGATGTGGTCCCCATGAAC
NM_001278497.1      TCCCAGGGCTGCGGGGAGGGCCAAGTGGCCTGTCTCTTTGAGGATGTGGTCCCCATGAAC
NM_001278499.1      TCCCAGGGCTGCGGGGAGGGCCAAGTGGCCTGTCTCTTTGAGGATGTGGTCCCCATGAAC
                    ** **.**.** ** **.** ***** ** ** ********.** **.** ** ******

HsCD00292958        TATATGGTCTACTTCAACTTCTTTGCTTGCGTCCTGGTGCCGCTGCTCTTGATGCTTGGT
NM_001278500.1      TACATGGTGTACTTCAACTTCTTTGCCTGTGTGCTGGTGCCCCTGCTGCTCATGCTGGGT
NM_000675.5         TACATGGTGTACTTCAACTTCTTTGCCTGTGTGCTGGTGCCCCTGCTGCTCATGCTGGGT
NM_001278498.1      TACATGGTGTACTTCAACTTCTTTGCCTGTGTGCTGGTGCCCCTGCTGCTCATGCTGGGT
NM_001278497.1      TACATGGTGTACTTCAACTTCTTTGCCTGTGTGCTGGTGCCCCTGCTGCTCATGCTGGGT
NM_001278499.1      TACATGGTGTACTTCAACTTCTTTGCCTGTGTGCTGGTGCCCCTGCTGCTCATGCTGGGT
                    ** ***** ***************** ** ** ******** *****  * ***** ***

HsCD00292958        GTATACCTCCGTATCTTCCTTGCCGCTAGACGTCAGCTTAAACAGATGGAGTCTCAACCT
NM_001278500.1      GTCTATTTGCGGATCTTCCTGGCGGCGCGACGACAGCTGAAGCAGATGGAGAGCCAGCCT
NM_000675.5         GTCTATTTGCGGATCTTCCTGGCGGCGCGACGACAGCTGAAGCAGATGGAGAGCCAGCCT
NM_001278498.1      GTCTATTTGCGGATCTTCCTGGCGGCGCGACGACAGCTGAAGCAGATGGAGAGCCAGCCT
NM_001278497.1      GTCTATTTGCGGATCTTCCTGGCGGCGCGACGACAGCTGAAGCAGATGGAGAGCCAGCCT
NM_001278499.1      GTCTATTTGCGGATCTTCCTGGCGGCGCGACGACAGCTGAAGCAGATGGAGAGCCAGCCT
                    **.**  * ** ******** ** ** .****:***** **.*********:  **.***

HsCD00292958        CTTCCTGGAGAGAGAGCGAGGTCAACTTTGCAGAAGGAGGTACATGCCGCCAAGTCATTG
NM_001278500.1      CTGCCGGGGGAGCGGGCACGGTCCACACTGCAGAAGGAGGTCCATGCTGCCAAGTCACTG
NM_000675.5         CTGCCGGGGGAGCGGGCACGGTCCACACTGCAGAAGGAGGTCCATGCTGCCAAGTCACTG
NM_001278498.1      CTGCCGGGGGAGCGGGCACGGTCCACACTGCAGAAGGAGGTCCATGCTGCCAAGTCACTG
NM_001278497.1      CTGCCGGGGGAGCGGGCACGGTCCACACTGCAGAAGGAGGTCCATGCTGCCAAGTCACTG
NM_001278499.1      CTGCCGGGGGAGCGGGCACGGTCCACACTGCAGAAGGAGGTCCATGCTGCCAAGTCACTG
                    ** ** **.***.*.**..****.**: *************.***** ********* **

HsCD00292958        GCTATCATCGTCGGTCTTTTTGCGCTGTGCTGGCTGCCCCTCCACATCATCAACTGTTTT
NM_001278500.1      GCCATCATTGTGGGGCTCTTTGCCCTCTGCTGGCTGCCCCTACACATCATCAACTGCTTC
NM_000675.5         GCCATCATTGTGGGGCTCTTTGCCCTCTGCTGGCTGCCCCTACACATCATCAACTGCTTC
NM_001278498.1      GCCATCATTGTGGGGCTCTTTGCCCTCTGCTGGCTGCCCCTACACATCATCAACTGCTTC
NM_001278497.1      GCCATCATTGTGGGGCTCTTTGCCCTCTGCTGGCTGCCCCTACACATCATCAACTGCTTC
NM_001278499.1      GCCATCATTGTGGGGCTCTTTGCCCTCTGCTGGCTGCCCCTACACATCATCAACTGCTTC
                    ** ***** ** ** ** ***** ** **************.************** ** 

HsCD00292958        ACCTTCTTCTGTCCCGACTGTTCACACGCACCTCTTTGGCTGATGTACCTGGCGATCGTT
NM_001278500.1      ACTTTCTTCTGCCCCGACTGCAGCCACGCCCCTCTCTGGCTCATGTACCTGGCCATCGTC
NM_000675.5         ACTTTCTTCTGCCCCGACTGCAGCCACGCCCCTCTCTGGCTCATGTACCTGGCCATCGTC
NM_001278498.1      ACTTTCTTCTGCCCCGACTGCAGCCACGCCCCTCTCTGGCTCATGTACCTGGCCATCGTC
NM_001278497.1      ACTTTCTTCTGCCCCGACTGCAGCCACGCCCCTCTCTGGCTCATGTACCTGGCCATCGTC
NM_001278499.1      ACTTTCTTCTGCCCCGACTGCAGCCACGCCCCTCTCTGGCTCATGTACCTGGCCATCGTC
                    ** ******** ******** : .*****.***** ***** *********** ***** 

HsCD00292958        CTCAGCCACACCAATTCGGTCGTAAATCCGTTTATCTATGCTTACCGTATTCGTGAATTT
NM_001278500.1      CTCTCCCACACCAATTCGGTTGTGAATCCCTTCATCTACGCCTACCGTATCCGCGAGTTC
NM_000675.5         CTCTCCCACACCAATTCGGTTGTGAATCCCTTCATCTACGCCTACCGTATCCGCGAGTTC
NM_001278498.1      CTCTCCCACACCAATTCGGTTGTGAATCCCTTCATCTACGCCTACCGTATCCGCGAGTTC
NM_001278497.1      CTCTCCCACACCAATTCGGTTGTGAATCCCTTCATCTACGCCTACCGTATCCGCGAGTTC
NM_001278499.1      CTCTCCCACACCAATTCGGTTGTGAATCCCTTCATCTACGCCTACCGTATCCGCGAGTTC
                    ***: *************** **.***** ** ***** ** ******** ** **.** 

HsCD00292958        CGCCAGACGTTTCGCAAGATCATCAGGTCCCATGTCCTCAGACAGCAAGAACCTTTCAAG
NM_001278500.1      CGCCAGACCTTCCGCAAGATCATTCGCAGCCACGTCCTGAGGCAGCAAGAACCTTTCAAG
NM_000675.5         CGCCAGACCTTCCGCAAGATCATTCGCAGCCACGTCCTGAGGCAGCAAGAACCTTTCAAG
NM_001278498.1      CGCCAGACCTTCCGCAAGATCATTCGCAGCCACGTCCTGAGGCAGCAAGAACCTTTCAAG
NM_001278497.1      CGCCAGACCTTCCGCAAGATCATTCGCAGCCACGTCCTGAGGCAGCAAGAACCTTTCAAG
NM_001278499.1      CGCCAGACCTTCCGCAAGATCATTCGCAGCCACGTCCTGAGGCAGCAAGAACCTTTCAAG
                    ******** ** *********** .* : *** ***** **.******************

HsCD00292958        GCTGCCGGCACCTCCGCCAGGGTCTTGGCGGCGCACGGAAGCGACGGTGAGCAAGTGTCT
NM_001278500.1      GCAGCTGGCACCAGTGCCCGGGTCTTGGCAGCTCATGGCAGTGACGGAGAGCAGGTCAGC
NM_000675.5         GCAGCTGGCACCAGTGCCCGGGTCTTGGCAGCTCATGGCAGTGACGGAGAGCAGGTCAGC
NM_001278498.1      GCAGCTGGCACCAGTGCCCGGGTCTTGGCAGCTCATGGCAGTGACGGAGAGCAGGTCAGC
NM_001278497.1      GCAGCTGGCACCAGTGCCCGGGTCTTGGCAGCTCATGGCAGTGACGGAGAGCAGGTCAGC
NM_001278499.1      GCAGCTGGCACCAGTGCCCGGGTCTTGGCAGCTCATGGCAGTGACGGAGAGCAGGTCAGC
                    **:** ******:  ***.**********.** ** **.** *****:*****.** :  

HsCD00292958        TTGCGCCTTAACGGACACCCTCCCGGTGTCTGGGCGAACGGCTCTGCCCCACACCCCGAG
NM_001278500.1      CTCCGTCTCAACGGCCACCCGCCAGGAGTGTGGGCCAACGGCAGTGCTCCCCACCCTGAG
NM_000675.5         CTCCGTCTCAACGGCCACCCGCCAGGAGTGTGGGCCAACGGCAGTGCTCCCCACCCTGAG
NM_001278498.1      CTCCGTCTCAACGGCCACCCGCCAGGAGTGTGGGCCAACGGCAGTGCTCCCCACCCTGAG
NM_001278497.1      CTCCGTCTCAACGGCCACCCGCCAGGAGTGTGGGCCAACGGCAGTGCTCCCCACCCTGAG
NM_001278499.1      CTCCGTCTCAACGGCCACCCGCCAGGAGTGTGGGCCAACGGCAGTGCTCCCCACCCTGAG
                     * ** ** *****.***** **.**:** ***** ******: *** **.***** ***

HsCD00292958        AGACGCCCCAATGGTTACGCTCTGGGACTTGTGTCTGGCGGATCAGCTCAGGAAAGCCAA
NM_001278500.1      CGGAGGCCCAATGGCTATGCCCTGGGGCTGGTGAGTGGAGGGAGTGCCCAAGAGTCCCAG
NM_000675.5         CGGAGGCCCAATGGCTATGCCCTGGGGCTGGTGAGTGGAGGGAGTGCCCAAGAGTCCCAG
NM_001278498.1      CGGAGGCCCAATGGCTATGCCCTGGGGCTGGTGAGTGGAGGGAGTGCCCAAGAGTCCCAG
NM_001278497.1      CGGAGGCCCAATGGCTATGCCCTGGGGCTGGTGAGTGGAGGGAGTGCCCAAGAGTCCCAG
NM_001278499.1      CGGAGGCCCAATGGCTATGCCCTGGGGCTGGTGAGTGGAGGGAGTGCCCAAGAGTCCCAG
                    .*..* ******** ** ** *****.** ***: ***.**.: :** **.**.: ***.

HsCD00292958        GGCAACACTGGTTTGCCTGACGTCGAACTGCTGTCGCACGAGTTGAAGGGCGTTTGCCCG
NM_001278500.1      GGGAACACGGGCCTCCCAGACGTGGAGCTCCTTAGCCATGAGCTCAAGGGAGTGTGCCCA
NM_000675.5         GGGAACACGGGCCTCCCAGACGTGGAGCTCCTTAGCCATGAGCTCAAGGGAGTGTGCCCA
NM_001278498.1      GGGAACACGGGCCTCCCAGACGTGGAGCTCCTTAGCCATGAGCTCAAGGGAGTGTGCCCA
NM_001278497.1      GGGAACACGGGCCTCCCAGACGTGGAGCTCCTTAGCCATGAGCTCAAGGGAGTGTGCCCA
NM_001278499.1      GGGAACACGGGCCTCCCAGACGTGGAGCTCCTTAGCCATGAGCTCAAGGGAGTGTGCCCA
                    ** ***** **  * **:***** **.** ** :  ** *** * *****.** *****.

HsCD00292958        GAGCCGCCTGGCTTGGACGACCCCTTGGCACAGGACGGTGCTGGCGTTTCGCACCACCAT
NM_001278500.1      GAGCCCCCTGGCCTAGATGACCCCCTGGCCCAGGATGGAGCAGGAGTGTCCTGA------
NM_000675.5         GAGCCCCCTGGCCTAGATGACCCCCTGGCCCAGGATGGAGCAGGAGTGTCCTGA------
NM_001278498.1      GAGCCCCCTGGCCTAGATGACCCCCTGGCCCAGGATGGAGCAGGAGTGTCCTGA------
NM_001278497.1      GAGCCCCCTGGCCTAGATGACCCCCTGGCCCAGGATGGAGCAGGAGTGTCCTGA------
NM_001278499.1      GAGCCCCCTGGCCTAGATGACCCCCTGGCCCAGGATGGAGCAGGAGTGTCCTGA------
                    ***** ****** *.** ****** ****.***** **:**:**.** **  ..      

HsCD00292958        CACCATCACCATCACCATCACTGAAAGCTTCTCGAG
NM_001278500.1      ------------------------------------
NM_000675.5         ------------------------------------
NM_001278498.1      ------------------------------------
NM_001278497.1      ------------------------------------
NM_001278499.1      ------------------------------------

Pathways

NCI :

HIF-2-alpha transcription factor network

Reactome :

Activation of TRKA receptors

Reactome :

Adenosine P1 receptors

Reactome :

Class A/1 (Rhodopsin-like receptors)

Reactome :

G alpha (s) signalling events

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

NGF signalling via TRKA from the plasma membrane

Reactome :

NGF-independant TRKA activation

Reactome :

Nucleotide-like (purinergic) receptors

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

Reactome :

Signalling by NGF

WikiPathway :

GPCRs, Class A Rhodopsin-like

WikiPathway :

GPCRs, Other

WikiPathway :

Monoamine Transport

WikiPathway :

Nucleotide GPCRs

Protein

PDB - Structural Annotation :

4EIY

PDB - Structural Annotation :

3EML

PDB - Structural Annotation :

3QAK

Structural Annotation Wiki :

TOPSAN

UniProt :

B3KVQ4

UniProt :

P29274

HPRD :

00043

Protein Structure Viewer

2D Image
3D Image (Jmol)

FirstGlance in Jmol

PDB ID

Style

Color

Clone

Cloning Information :

485322

HIP Master Clone ID :

401879

Original Clone ID :

ATCG3D_48

Experimental

Structural Biology Knowledgebase :

SBKB (4EIY)

Structural Biology Knowledgebase :

SBKB (3EML)

Structural Biology Knowledgebase :

SBKB (3QAK)

TargetTrack - Experimental data :

ATCG3D_5

TargetTrack - Experimental data :

GPCR-6

Organism

Species Specific ID:

135

Genome

Chromosome :

Map Location :

22q11.23

Ensembl :

ENSG00000128271

Reagents

Labome :

ADORA2A-antibody

ADORA2A (H. sapiens )In pET28a (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

ADORA2A (H. sapiens )
In pET28a (His-tagged bacterial expression vector)