Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Publications:
None
Authors:
University of California San Francisco
Marta Pioletti
Center for Structures of Membrane Proteins
EvNO00292960
Price:
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AVAILABLE
No restriction
Special MTA:
PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Bacterial expression vector with N-terminal 8x His tag separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Size (bp):
5382
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of California San Francisco
Marta Pioletti
Center for Structures of Membrane Proteins
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
5162
5167
phage origin
f1
f1 phage origin
29
335
promoter
T7
T7 promoter
4984
5003
protease cleavage site
3C
3C protease cleavage site; separates the His tag from the insert