Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Publications:
None
Authors:
University of California San Francisco
Marta Pioletti
Center for Structures of Membrane Proteins
EvNO00292962
Price:
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AVAILABLE
No restriction
Special MTA:
PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Size (bp):
6528
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of California San Francisco
Marta Pioletti
Center for Structures of Membrane Proteins
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
ligation independent cloning
LIC
Ligation independent cloning site
0
0
phage origin
f1
f1 phage origin
0
0
promoter
T7
T7 promoter
0
0
protease cleavage site
3C
3C protease cleavage site; separates the 6xHis tag and MBP from the insert