Detailed Vector Information: pHM3C-LIC

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pHM3C-LIC
Source:
Center for Structures of Membrane Proteins
Description:
Bacterial expression vector with N-terminal 6xHis and MBP tags separated from the insert by a 3C protease cleavage site; kanamycin resistance in bacteria; ligation independent cloning (LIC)
Comments:
None
Publications:
None
Authors:
University of California San Francisco
Marta Pioletti
Center for Structures of Membrane Proteins
EvNO00292962
Price: 
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No restriction
Special MTA: PSI

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.