Gene:
Gene Symbol:
Ptprg
Gene Name:
protein tyrosine phosphatase, receptor type, G
Original Clone ID:
FLH481102.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00294847
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4329nts
Open reading frame: 35 to 4363
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCGGAGGTTACTGGAACCGTGTTG
GTGGATTTTGTTCCTGAAAATCACCAGTTCTGTGCTTCATTATGTGGTGTGCTTCCCGGC
ATTGACTGAAGGCTATGTGGGGACCCTGCAGGAGAGCAGACAGGACAGCTCAGTGCAGAT
CCGCAGACGAAAGGCATCCGGAGACCCATACTGGGCGTATTCTGGTGCCTATGGTCCTGA
GCACTGGGTCACATCTAGTGTCAGCTGCGGGGGCAGCCATCAGTCTCCTATAGACATTTT
AGACCACCATGCTCGCGTTGGCGATGAATACCAGGAGCTTCAACTTGATGGGTTTGACAA
CGAGTCCTCTAACAAAACCTGGATGAAAAATACAGGGAAAACAGTTGCCATCCTGCTGAA
AGACGATTATTTTGTGAGTGGTGCCGGACTGCCGGGCAGATTCAAAGCTGAGAAGGTGGA
GTTTCACTGGGGCCACAGCAATGGCTCCGCTGGCTCAGAGCATAGTGTCAATGGCCGGAG
GTTTCCTGTGGAGATGCAGATTTTCTTTTACAACCCAGACGACTTTGACAGCTTTCAAAC
GGCAATTTCTGAGAACAGAATAATTGGAGCTATGGCCATATTTTTCCAAGTCAGTCCGAG
GGACAATTCTGCACTGGATCCTATTATCCACGGGCTGAAGGGCGTCGTACATCACGAGAA
GGAGACTTTCCTGGATCCTTTCATTCTCCGAGACCTCCTGCCCGCATCCCTGGGGAGTTA
TTACAGGTACACAGGCTCCTTGACTACGCCACCCTGTAGTGAAATAGTGGAGTGGATTGT
CTTCCGGAGGCCCGTTCCCATCTCCTATCACCAGCTTGAGGCTTTTTATTCTATCTTCAC
CACGGAGCAGCAAGACCATGTCAAGTCAGTGGAGTACCTGAGAAATAACTTCCGACCACA
ACAAGCTCTGAATGACAGAGTGGTGTCAAAGTCTGCTGTCCGGGATGCCTGGAACCATGA
CCTGGCAGACTTCTTGGACAACCCACTAGGCACAGAAGCCTCTAAAGTTTGCAGCTCTCC
GCCCATCCACATGAAGGTGCAGCCTCTGAACCAGACAGCTCTGCAGGTGTCCTGGAGCCA
ACCTGAAACCATCTACCACCCGCCCATCATGAATTACATGATCTCCTATAGCTGGACCAA
GAATGAGGACGAGAAGGAGAAGACATTTACAAAAGACAGTGACAAGGACTTGAAAGCCAC
CATCAGCCATGTCTCACCCGATAGCCTTTACCTATTCCGAGTACAGGCTGTGTGTAGGAA
TGACATGCGGAGTGACTTCAGCCAGACAATGCTCTTTCAAGCTAATACTACCCGGATCTT
CCAAGGGACCAGGATTGTGAAAACAGGAGTGCCGACAGCCTCTCCTGCCTCTTCAGCAGA
CATGGCCCCCATCAGCTCTGGCTCTTCCACTTGGACATCCTCCGGCATCCCCTTCTCATT
TGTTTCCATGGCAACTGGGATGGGCCCTTCCTCCAGTGGCAGCCAAGCTACAGTGGCCTC
TGTGGTCACCAGCACACTGTTGGCAGGCCTGGGCTTCGGTGGTGGTGGCATCTCCTCCTT
CCCCAGTACCGTGTGGCCCACGCGCCTTCCCACCGCATCTGCAGCCAGCAAGCAGGCAGG
GAGGACAGTTCTTGCCACCACCGAGGCCTTAGCTTCCCCTGGGCCGGATGTCGACTCGGC
ACCGACGAAGGACAGCGAGGGTACAGAGGAGGGAGAGAAGGAGGAGAAAAGCGAGAGCGA
GGATGGTGAGCGAGAGCATGAGGAGGAAGAGAAGGACTCTGAGAAGAAGGAGAAGAGTGA
GGCTACTCACACAGCCGCAGAGAGTGACCGGACTGCACCTGCCCCCACCCCCTCTTCCCC
TCATAGGACTGCAGCGGAGGGAGGACATCAGACTATACCTGGGCGCAGGCAGGACCACTC
TGCCCCTGCCACAGACCAGCCGGGCCACGTTGCCCCAGACTTGGATCCCCTTGTGGACAC
AGCCACCCAAGTGCCCCCAACAGCCACAGAGGAACATTATTCAGGGAGTGATCCCAGGAG
GCCCGAAATGCCATCTAAAAAGCCTATGTCCCGAGGGGACCGATTTTCTGAGGATAGCAA
ATTCATCACAGTTAACCCAGCTGAGAAGAACACCTCTGGCATGCTAAGCCGCCCCTCCCC
CGGGAGGATGGAGTGGATCATCCCCCTGATTGTGGTCTCAGCCTTGACCTTCGTGTGCCT
CGTCCTCCTCATTGCCGTGCTGGTGTACTGGAGAGGGTGTAACAAAATAAAGTCCAAGGG
CTTTCCCCGACGTTCCCGTGAAGTGCCTTCTTCTGGGGAGAGAGGAGAGAAGGGGAGCAG
AAAATGTTTTCAGACTGCTCATTTCTATGTGGAAGACAGCAGTTCACCTCGGGTGGTCCC
CAACGAAAGTGTTCCTATTATTCCCATCCCGGATGACATGGAAGCCATTCCTGTCAAACA
GTTTGGTAAACACATCGGTGAGCTCTATTCCAATAGCCAGCATGGCTTCTCGGAAGATTT
TGAGGAAGTCCAGCGCTGTACAGCTGATATGAACATCACTGCAGAGCATTCCAATCATCC
GGATAACAAGCACAAAAACAGATACATCAACATTTTAGCATATGATCACAGTAGGGTGAA
GTTAAGACCTTTACCAGGAAAGGACTCTAAGCACAGCGACTACATTAATGCAAACTATGT
CGATGGTTACAACAAAGCGAAAGCCTACATTGCCACCCAGGGACCTTTAAAATCCACCTT
TGAAGACTTCTGGAGGATGATCTGGGAACAAAACACGGGAATCATCATCATGATCACGAA
CCTCGTGGAGAAAGGAAGGAGAAAGTGTGATCAGTATTGGCCAACGGAGAACACTGAGGA
GTACGGGAACATTATTGTCACACTGAAGAGCACAAAAGTACATGCCTGCTACACCGTCCG
TCGTCTTTCAGTTAGAAACACAAAAGTGAAAAAGGGCCAGAAGGGCAACCCCAAAGGTCG
TCAGAATGAGCGGACGGTGATCCAGTACCACTACACACAGTGGCCTGACATGGGTGTCCC
TGAGTACGCCCTCCCAGTCCTGACCTTCGTGAGGAGGTCATCTGCGGCCCGCATGCCAGA
CATGGGGCCCGTGTTGGTGCACTGCAGCGCTGGTGTGGGCAGGACAGGCACCTACATTGT
AATAGACAGCATGCTGCAGCAGATCAAAGACAAAAGCACAGTTAATGTCCTGGGCTTCCT
GAAGCATATCAGGACACAGAGGAACTACCTCGTCCAGACTGAGGAGCAGTACATTTTCAT
CCATGATGCCTTGTTGGAAGCCATTCTCGGGAAGGAAACTGAAGTATCTTCAAGTCAGCT
GCATAGCTATGTTAACAGCATCCTCATACCAGGAGTAGGAGGAAAGACACGGCTGGAAAA
GCAGTTTAAGCTGATCACGCAGTGTAATGCAAAGTATGTGGAGTGCTTCAGTGCCCAGAA
GGAGTGCAACAAAGAGAAGAACAGGAACTCGTCTGTTGTGCCAGCTGAGCGTGCTCGAGT
GGGACTTGCACCGTTGCCCGGGATGAAAGGAACAGATTACATTAATGCTTCTTACATCAT
GGGGTATTATAGAAGCAACGAGTTCATCATAACCCAGCATCCTCTGCCACACACTACGAA
AGATTTCTGGAGAATGATTTGGGATCATAATGCACAGATCATTGTCATGCTGCCAGACAA
CCAGAGCCTGGCAGAAGATGAGTTTGTGTACTGGCCGAGTCGTGAGGAATCCATGAACTG
TGAGGCCTTTACTGTCACCCTCATCAGCAAAGACAGGCTATGCCTCTCGAATGAAGAACA
AATTATCATCCATGACTTCATCCTTGAAGCTACACAGGATGACTACGTACTAGAGGTTCG
GCACTTTCAGTGTCCCAAATGGCCTAACCCAGATGCCCCTATAAGTAGTACCTTCGAACT
GATCAATGTCATCAAGGAAGAGGCCTTAACCCGGGATGGCCCCACCATTGTTCATGATGA
GTATGGAGCAGTTTCTGCAGGAATGTTGTGTGCCCTTACCACCTTGTCCCAGCAGCTAGA
GAATGAAAATGCTGTGGATGTGTTCCAGGTGGCAAAAATGATCAATCTTATGAGACCTGG
AGTATTCACAGACATTGAACAATACCAGTTTGTCTACAAAGCAATGCTCAGCTTGATCAG
TACTAAAGAAAATGGAAATGGTCCCATGACCGGGGACAAAAACGGTGCTGTGCTAACTGC
AGAGGAATCAGACCCTGCAGAGAGCATGGAGTCTCTGGTGTAGGGCCTCATGGGCCCAGC
TTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MRRLLEPCWWILFLKITSSVLHYVVCFPALTEGYVGTLQESRQDSSVQIRRRKASGDPYW AYSGAYGPEHWVTSSVSCGGSHQSPIDILDHHARVGDEYQELQLDGFDNESSNKTWMKNT GKTVAILLKDDYFVSGAGLPGRFKAEKVEFHWGHSNGSAGSEHSVNGRRFPVEMQIFFYN PDDFDSFQTAISENRIIGAMAIFFQVSPRDNSALDPIIHGLKGVVHHEKETFLDPFILRD LLPASLGSYYRYTGSLTTPPCSEIVEWIVFRRPVPISYHQLEAFYSIFTTEQQDHVKSVE YLRNNFRPQQALNDRVVSKSAVRDAWNHDLADFLDNPLGTEASKVCSSPPIHMKVQPLNQ TALQVSWSQPETIYHPPIMNYMISYSWTKNEDEKEKTFTKDSDKDLKATISHVSPDSLYL FRVQAVCRNDMRSDFSQTMLFQANTTRIFQGTRIVKTGVPTASPASSADMAPISSGSSTW TSSGIPFSFVSMATGMGPSSSGSQATVASVVTSTLLAGLGFGGGGISSFPSTVWPTRLPT ASAASKQAGRTVLATTEALASPGPDVDSAPTKDSEGTEEGEKEEKSESEDGEREHEEEEK DSEKKEKSEATHTAAESDRTAPAPTPSSPHRTAAEGGHQTIPGRRQDHSAPATDQPGHVA PDLDPLVDTATQVPPTATEEHYSGSDPRRPEMPSKKPMSRGDRFSEDSKFITVNPAEKNT SGMLSRPSPGRMEWIIPLIVVSALTFVCLVLLIAVLVYWRGCNKIKSKGFPRRSREVPSS GERGEKGSRKCFQTAHFYVEDSSSPRVVPNESVPIIPIPDDMEAIPVKQFGKHIGELYSN SQHGFSEDFEEVQRCTADMNITAEHSNHPDNKHKNRYINILAYDHSRVKLRPLPGKDSKH SDYINANYVDGYNKAKAYIATQGPLKSTFEDFWRMIWEQNTGIIIMITNLVEKGRRKCDQ YWPTENTEEYGNIIVTLKSTKVHACYTVRRLSVRNTKVKKGQKGNPKGRQNERTVIQYHY TQWPDMGVPEYALPVLTFVRRSSAARMPDMGPVLVHCSAGVGRTGTYIVIDSMLQQIKDK STVNVLGFLKHIRTQRNYLVQTEEQYIFIHDALLEAILGKETEVSSSQLHSYVNSILIPG VGGKTRLEKQFKLITQCNAKYVECFSAQKECNKEKNRNSSVVPAERARVGLAPLPGMKGT DYINASYIMGYYRSNEFIITQHPLPHTTKDFWRMIWDHNAQIIVMLPDNQSLAEDEFVYW PSREESMNCEAFTVTLISKDRLCLSNEEQIIIHDFILEATQDDYVLEVRHFQCPKWPNPD APISSTFELINVIKEEALTRDGPTIVHDEYGAVSAGMLCALTTLSQQLENENAVDVFQVA KMINLMRPGVFTDIEQYQFVYKAMLSLISTKENGNGPMTGDKNGAVLTAEESDPAESMES LV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
5024
Start on reference sequence
696
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
