Gene:
Gene Symbol:
Ksr1
Gene Name:
kinase suppressor of ras 1
Original Clone ID:
FLH481103.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00294848
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2622nts
Open reading frame: 35 to 2656
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATAGAGCGGCGTTGCGCGCGGC
AGCGATGGGCGAGAAAAAGGAGGGCGGCGGCGGGGGCGCCGCGGCGGACGGGGGCGCAGG
GGCCGCCGTCAGCCGGGCGCTGCAGCAGTGCGGCCAGCTGCAGAAGCTCATCGATATCTC
CATCGGCAGTCTGCGCGGGCTGCGCACCAAGTGCTCAGTGTCTAACGACCTCACACAGCA
GGAGATCCGGACCCTAGAGGCAAAGCTGGTGAAATACATTTGCAAGCAGCAGCAGAGCAA
GCTTAGTGTGACCCCAAGCGACAGGACCGCCGAGCTCAACAGCTACCCACGCTTCAGTGA
CTGGCTGTACATCTTCAACGTGAGGCCTGAGGTGGTGCAGGAGATCCCCCAAGAGCTCAC
ACTGGATGCTCTGCTGGAGATGGACGAGGCCAAAGCCAAGGAGATGCTGCGGCGCTGGGG
GGCCAGCACGGAGGAGTGCAGCCGCCTACAGCAAGCCCTTACCTGCCTTCGGAAGGTGAC
TGGCCTGGGAGGGGAGCACAAAATGGACTCAGGTTGGAGTTCAACAGATGCTCGAGACAG
TAGCTTGGGGCCTCCCATGGACATGCTTTCCTCGCTGGGCAGAGCGGGTGCCAGCACTCA
GGGACCCCGTTCCATCTCCGTGTCCGCCCTGCCTGCCTCAGACTCTCCGGTCCCCGGCCT
CAGTGAGGGCCTCTCGGACTCCTGTATCCCCTTGCACACCAGCGGCCGGCTGACCCCCCG
GGCCCTGCACAGCTTCATCACGCCCCCTACCACACCCCAGCTACGACGGCACGCCAAGCT
GAAGCCACCAAGGACACCCCCACCGCCAAGCCGCAAGGTCTTCCAGCTGCTCCCCAGCTT
CCCCACACTCACACGGAGCAAGTCCCACGAGTCCCAGCTGGGAAACCGAATCGACGACGT
CACCCCGATGAAGTTTGAACTCCCTCATGGATCCCCACAGCTGGTACGAAGGGATATCGG
GCTCTCGGTGACGCACAGGTTCTCCACAAAGTCATGGTTGTCACAGGTGTGCAACGTGTG
CCAGAAGAGCATGATTTTTGGCGTGAAGTGCAAACACTGCAGGTTAAAATGCCATAACAA
GTGCACAAAGGAAGCTCCCGCCTGCAGGATCACCTTCCTCCCACTGGCCAGGCTTCGGAG
GACAGAGTCTGTCCCGTCAGATATCAACAACCCAGTGGACAGAGCAGCAGAGCCCCATTT
TGGAACCCTTCCCAAGGCCCTGACAAAGAAGGAGCACCCTCCAGCCATGAACCTGGACTC
CAGCAGCAACCCATCCTCCACCACGTCCTCCACACCCTCATCGCCGGCACCTTTCCTGAC
CTCATCTAATCCCTCCAGTGCCACCACGCCTCCCAACCCGTCACCTGGCCAGCGGGACAG
CAGGTTCAGCTTCCCAGACATTTCAGCCTGTTCTCAGGCAGCCCCGCTGTCCAGCACAGC
CGACAGTACACGGCTCGACGACCAGCCCAAAACAGATGTGCTAGGTGTTCACGAAGCAGA
GGCTGAGGAGCCTGAGGCTGGCAAGTCAGAGGCAGAGGATGACGAGGAGGATGAGGTGGA
CGACCTCCCCAGCTCCCGCCGGCCCTGGAGGGGCCCCATCTCTCGAAAGGCCAGCCAGAC
CAGCGTTTACCTGCAAGAGTGGGACATCCCCTTTGAACAGGTGGAACTGGGCGAGCCCAT
TGGACAGGGTCGCTGGGGCCGGGTGCACCGAGGCCGTTGGCATGGCGAGGTGGCCATTCG
GCTGCTGGAGATGGACGGCCACAATCAGGACCACCTGAAGCTGTTCAAGAAAGAGGTGAT
GAACTACCGGCAGACGCGGCATGAGAACGTGGTGCTCTTCATGGGGGCCTGCATGAACCC
ACCTCACCTGGCCATTATCACCAGCTTCTGCAAGGGGCGGACATTGCATTCATTCGTGAG
GGACCCCAAGACGTCTCTGGACATCAATAAGACTAGGCAGATCGCCCAGGAGATCATCAA
GGGCATGGGTTATCTTCATGCAAAAGGCATCGTGCACAAGGACCTCAAGTCCAAGAATGT
CTTCTATGACAACGGCAAAGTGGTCATCACAGACTTCGGGCTGTTTGGGATCTCGGGTGT
GGTCCGAGAGGAACGGCGCGAGAACCAACTGAAACTGTCACATGACTGGCTGTGCTACCT
GGCCCCCGAGATCGTACGAGAAATGATCCCGGGGCGGGACGAGGACCAGCTGCCCTTCTC
CAAAGCAGCCGATGTCTATGCATTCGGGACTGTGTGGTATGAACTACAGGCAAGAGACTG
GCCCTTTAAGCACCAGCCTGCTGAGGCCTTGATCTGGCAGATTGGAAGTGGGGAAGGAGT
ACGGCGCGTCCTGGCATCCGTCAGCCTGGGGAAGGAAGTCGGCGAGATCCTGTCTGCCTG
CTGGGCTTTCGATCTGCAGGAGAGACCCAGCTTCAGCCTGCTGATGGACATGCTGGAGAG
GCTGCCCAAGCTGAACCGGCGGCTCTCCCACCCTGGGCACTTTTGGAAGTCGGCTGACAT
TAACAGCAGCAAAGTCATGCCCCGCTTTGAAAGGTTTGGCCTGGGGACCCTGGAGTCCGG
TAATCCAAAGATGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDRAALRAAAMGEKKEGGGGGAAADGGAGAAVSRALQQCGQLQKLIDISIGSLRGLRTKC SVSNDLTQQEIRTLEAKLVKYICKQQQSKLSVTPSDRTAELNSYPRFSDWLYIFNVRPEV VQEIPQELTLDALLEMDEAKAKEMLRRWGASTEECSRLQQALTCLRKVTGLGGEHKMDSG WSSTDARDSSLGPPMDMLSSLGRAGASTQGPRSISVSALPASDSPVPGLSEGLSDSCIPL HTSGRLTPRALHSFITPPTTPQLRRHAKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHES QLGNRIDDVTPMKFELPHGSPQLVRRDIGLSVTHRFSTKSWLSQVCNVCQKSMIFGVKCK HCRLKCHNKCTKEAPACRITFLPLARLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKE HPPAMNLDSSSNPSSTTSSTPSSPAPFLTSSNPSSATTPPNPSPGQRDSRFSFPDISACS QAAPLSSTADSTRLDDQPKTDVLGVHEAEAEEPEAGKSEAEDDEEDEVDDLPSSRRPWRG PISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDH LKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKT RQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREERRENQLK LSHDWLCYLAPEIVREMIPGRDEDQLPFSKAADVYAFGTVWYELQARDWPFKHQPAEALI WQIGSGEGVRRVLASVSLGKEVGEILSACWAFDLQERPSFSLLMDMLERLPKLNRRLSHP GHFWKSADINSSKVMPRFERFGLGTLESGNPKM*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2697
Start on reference sequence
76
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
