Gene:
Gene Symbol:
Gigyf1
Gene Name:
GRB10 interacting GYF protein 1
Original Clone ID:
FLH481106.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00294851
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3135nts
Open reading frame: 35 to 3169
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGCAGCAGAGACACTCAACTTTGG
GCCTGAGTGGCTGAGAGCCCTTTCGAGTGGTGGCAGCGTGGCCTCTCCACCCCCATCTCC
TGCCATGCCCAAATACAAGCTGGCAGATTACCGCTATGGGCGAGAGGAGATGCTGGCCCT
CTATGTCAAGGAGAACAAGGTCCCGGAGGAACTACAGGACAAGGAGTTTGCTGCAGTGTT
ACAAGAGGAACCGCTGCAACCTCTGGCTCTGGAGCCTCTGACTGAGGAGGAGCAGAGAAA
CTTCTCGCTGTCGGTGAACAGTGTGGCTGTGCTGAGACTGATGGGGAAAGGGGCTGGGCC
CCCTTTGCCTGCCACCTCCCGTGGCAGGGGCAGCACTCGGAGCCGAGGGCGAGGCCGTGG
TGACAGCTGCTTTTACCAAAGAAGCATTGAGGAAGGCGATGGGGCCTTCGGAAGGAATCC
TCGGGAGATCCAGCGAAGCCAAAGCTGGGATGACAGAGGCGAGAGGCGGTTTGAGAAGCC
TGCAAGGAGGGATGGAGTACGGTCTGGCTTTGAGGAGGGAGGGGCTGGCCCAAGGAAGGA
GCATGCACGCTCAGACAGTGAGAATTGGCGCTCACTTCGAGAGGAGCAAGAGGATGATGG
CAGCTGGAGACTTGGGGCGGGACCCCGGCGAGATGGTGATCGCTGGCGTTCCACCAGCCC
TGATGGTGGTCCCCGCTCTGCTGGCTGGCGGGAGCATGGGGAGCGGAGGCGCAAGTTTGA
TTTTGATTTGCGAGGGGAGCGCGGAGGGTGTGGTGAAGAGGATGGGCGGGTTGGGGGCGG
CAACTCTCACCTCCGGAGATGCCGGGGGCTCGATGGCTTTGAAGATGACAAGGATGGGCT
CCCCGAGTGGTGCCTGGAGGATGAGGATGAGGAGATGGGCACTTTCGATGCCTCCGGAGC
CTTCCTGCCCCTTAAGAAGGGCCCCAAGGAAGCCATCCCTGAGGAGCAGGAGCTTGATTT
CCGGGGCCTGGAGGAGGAGGAGGAGGAGGAGGAGGAGCCGTCAGAAGGGGTGGATGAAGA
GAGGCCTGAGGCAGGAGGGAAGGAGGCAACCCCATTGCCGCCTCCAGAGAACTCCAGCTC
ACCGTCTTCACTGCCAGCCTTGGGTCCTCTCTGGACGACAAATGAGGAGGGTGGTGAGGC
AGTGGAGAAAGAGCTGCCTCCTGCTGAAGGTGACGAGTTGAGGGGACTGTCTCTGAGTCC
TAGAATCAGTTCACCTCCTGGCCCACCTGGAGATCTAGAAGACGAAGAAGGCTTGAAGCA
CTTGCAGCAGGAGGCAGAGAAGCTGGTGGCCTCCCTGCAGGACAGCTCCCTGGAGGAGGA
GCAGTTCACCGCTGCCATGCAAACCCAGGGCCTCCGCCACTCCACAGCTGCCACTGCCCT
TCCCCTCAGCCATGGCGCTGCCCGAAAGTGGTTCTACAAGGACCCACAGGGGGAAATCCA
AGGCCCTTTCACGACCCAGGAGATGGCTGAGTGGTTCCAGGCTGGCTACTTCTCCATGTC
ACTCTTAGTGAAGAGGGGCTGCGATGAGGGTTTCCAGCCTCTGGGAGAGGTGATCAAGAT
GTGGGGCCGTGTGCCCTTTGCCCCAGGGCCCTCGCCACCCCCGCTGCTGGGCAACATGGA
TCAGGAGCGGCTGAAGAAGCAGCAGGAGCTGGCTGCGGCAGCCCTGTACCAGCAGCTGCA
GCACCAGCACTTTCTGCAGCTGGTTGGCAGCCGCCAGCTCCCGCAGTGTACGACGCTCCG
GGAAAAGGCAGCTATGGGGGACCTGACGCCGCCGCAGCAGCAGCAGCTCACTACGTTCCT
GCAGCAGCTCCAGGCTCTCAAAACCCCCAGAGGTGGAGACCAGAACCTGCTCCCGACGAT
GAGCCGGTCCTTGTCGGTGCCAGATTCAGGCCCCCTCTGGGACTTACATACCTCAGCTTC
ATCACAGTCAGGTGGTGAGGCCAGTCTTTGGGACATACCAATTAACTCTTCGACTCAGGG
TCCAATTCTAGAACAGCTCCAACTGCAGCATAAATTTCAAGAGCGCAGAGAAGTGGAGCT
CAGGGCGAAGCGGGAGGAGGAGGAGCGCAAACGACGGGAGGAGAAGCGGCGCCAACAGCA
GCAGCAGCAGGAGGAGCAGAAGCGACGGCAGGAAGAGGAGGAGCTCTTTCGGCGCAAGCA
GGTACGGCAGCAGGAATTGTTGCTGAAGCTGCTACAGCAGCAGCAAGCGACGAATGTCCC
TGTGCCCCCTGCACCCAGCTCCCCACCCCCGCTCTGGGCTGGCCTAGCCAAGCAGGGCCT
GTCCATGAAGACTCTGCTGGAGCTGCAGATGGAAAGTGAGCGCCAACTGCACAAGCAGGC
GGCACCGCGGGAGCCTCTGCGGGCCCAGGCCCCCAACCACCGAGTGCAGCTTGGGGGCTT
GGGCTCTGCCCCTCTGAACCAGTGGGTGTCTGAGGCTGGGCCACTATGGGGCGGGCCAGA
CAAGAGTGGGGGCAGCAGCGGTGGCAACCTGGGACTCTGGGAAGATACTCTCAAGAGTGG
CGGGAGCCTGGCCCGAAGCCTGGGCCTGAAGAGCAGTCGGAGCAGCCCGTCTCTCAGTGA
CTCGTACAGCCACCTGTCAGGCCGACCTGTTCGCAAAAAGACAGAAGAGGAAGAGAAGTT
GCTGAAGCTGCTGCAGGGCATCCCTCGGCCCCAGGACGGCTTCACGCAGTGGTGTGAGCA
AATGCTGCACACCCTGAGCACTGCGGGCAGCCTAGACGTTCCCATGGCTGTCGCCATCCT
CAAGGAGGTGGAGTCCCCTTACGATGTCCATGATTATATTCGTTCCTGCCTGGGGGACAC
GCTGGAAGCCAAAGAATTTGCCAAACAATTCCTGGAGCGGAGGGCCAAGCAGAAAGCCAG
CCAACAGCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGAGGCCTGGCTGAG
CAGCACCTCCCTACAGACAGCCTTTCAGGCCAACCACAGCACCAAACTGGGCCCTGGGGA
GGGCAGCAAGGCCAAAAGGCGGGCACTGATGCTTCACTCCGACCCTAGCATCTTGGGGTA
CTCCCTGCACGGCCCTTCCGGTGAGATCGAGAGCGTGGATGACTACTAGGGCCTCATGGG
CCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MAAETLNFGPEWLRALSSGGSVASPPPSPAMPKYKLADYRYGREEMLALYVKENKVPEEL QDKEFAAVLQEEPLQPLALEPLTEEEQRNFSLSVNSVAVLRLMGKGAGPPLPATSRGRGS TRSRGRGRGDSCFYQRSIEEGDGAFGRNPREIQRSQSWDDRGERRFEKPARRDGVRSGFE EGGAGPRKEHARSDSENWRSLREEQEDDGSWRLGAGPRRDGDRWRSTSPDGGPRSAGWRE HGERRRKFDFDLRGERGGCGEEDGRVGGGNSHLRRCRGLDGFEDDKDGLPEWCLEDEDEE MGTFDASGAFLPLKKGPKEAIPEEQELDFRGLEEEEEEEEEPSEGVDEERPEAGGKEATP LPPPENSSSPSSLPALGPLWTTNEEGGEAVEKELPPAEGDELRGLSLSPRISSPPGPPGD LEDEEGLKHLQQEAEKLVASLQDSSLEEEQFTAAMQTQGLRHSTAATALPLSHGAARKWF YKDPQGEIQGPFTTQEMAEWFQAGYFSMSLLVKRGCDEGFQPLGEVIKMWGRVPFAPGPS PPPLLGNMDQERLKKQQELAAAALYQQLQHQHFLQLVGSRQLPQCTTLREKAAMGDLTPP QQQQLTTFLQQLQALKTPRGGDQNLLPTMSRSLSVPDSGPLWDLHTSASSQSGGEASLWD IPINSSTQGPILEQLQLQHKFQERREVELRAKREEEERKRREEKRRQQQQQQEEQKRRQE EEELFRRKQVRQQELLLKLLQQQQATNVPVPPAPSSPPPLWAGLAKQGLSMKTLLELQME SERQLHKQAAPREPLRAQAPNHRVQLGGLGSAPLNQWVSEAGPLWGGPDKSGGSSGGNLG LWEDTLKSGGSLARSLGLKSSRSSPSLSDSYSHLSGRPVRKKTEEEEKLLKLLQGIPRPQ DGFTQWCEQMLHTLSTAGSLDVPMAVAILKEVESPYDVHDYIRSCLGDTLEAKEFAKQFL ERRAKQKASQQRQQQQQQQQQQQQEAWLSSTSLQTAFQANHSTKLGPGEGSKAKRRALML HSDPSILGYSLHGPSGEIESVDDY*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3207
Start on reference sequence
73
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
