Gene:
Gene Symbol:
Ltbp3
Gene Name:
latent transforming growth factor beta binding protein 3
Original Clone ID:
FLH481118.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00294863
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3762nts
Open reading frame: 35 to 3796
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCCCGGGCCCCGAGGGGCTGCCCA
CGGCCTGGCCCCTGCGATGCGCCAGGCCGGGGCATTGGGGCTGCTGGCACTACTCCTGCT
GGCGCTGCTGGGCCCCGGCGGCGGGGCCGAGGGTGGGCCGGCCGGCGAGCGGGGCACAGG
CGGGGGCGGGGCGCTGGCCCGCGAACGCTTCAAGGTGGTCTTTGCGCCAGTGATCTGCAA
GCGGACCTGTCTGAAGGGCCAGTGTCGGGACAGCTGTCAGCAGGGCTCCAACATGACGCT
CATCGGAGAGAACGGCCACAGCACCGACACGCTCACCGGTTCTGGCTTCCGCGTGGTGGT
GTGCCCTCTACCCTGCATGAACGGTGGCCAGTGCTCTTCCCGAAACCAGTGCCTGTGTCC
CCCGGATTTCACGGGGCGCTTCTGCCAGGTGCCTGCTGCAGGAACCGGAGCTGGCACCGG
GAGTTCAGGCCCCGGCCTGGCCCGGACCGGGGCCATGTCCACAGGCCCGCTGCCGCCCCT
TGCCCCAGAAGGAGAGTCTGTGGCTAGCAAACACGCCATTTACGCGGTGCAGGTGATCGC
AGATCCTCCCGGGCCGGGGGAGGGTCCTCCTGCACAACATGCAGCCTTCTTGGTGCCCCT
GGGGCCAGGACAAATCTCGGCAGAAGTGCAGGCTCCGCCCCCCGTGGTGAACGTGCGTGT
CCATCACCCTCCTGAAGCTTCCGTTCAGGTGCACCGCATCGAGGGGCCGAACGCTGAAGG
CCCAGCCTCTTCCCAGCACTTGCTGCCGCATCCCAAGCCCCAGCACCCGAGGCCACCCAC
CCAAAAGCCACTGGGCCGCTGCTTCCAGGACACATTGCCCAAGCAGCCTTGTGGCAGCAA
CCCTTTGCCTGGCCTTACCAAGCAGGAAGATTGCTGCGGTAGCATCGGTACTGCCTGGGG
ACAAAGCAAGTGTCACAAGTGCCCACAGCTTCAGTATACAGGGGTGCAGAAGCCTGGACC
TGTACGTGGGGAGGTGGGTGCTGACTGCCCCCAGGGCTACAAGAGGCTCAACAGCACCCA
CTGCCAGGATATCAACGAATGTGCGATGCCTGGCATGTGTCGCCATGGTGACTGCCTCAA
CAACCCTGGCTCTTATCGCTGTGTCTGCCCGCCCGGTCATAGCTTGGGTCCCTCGCGCAC
ACAGTGCATTGCCGACAAACCAGAGGAGAAGAGCCTGTGTTTCCGCCTTGTGAGCACCGA
ACACCAGTGCCAGCACCCTCTGACCACACGCCTAACCCGCCAGCTCTGCTGCTGTAGTGT
GGGTAAAGCCTGGGGTGCCCGGTGCCAGCGCTGCCCGGCAGATGGTACAGCAGCCTTCAA
GGAGATCTGCCCGGCTGGGAAAGGGTACCATATCCTCACCTCCCACCAGACGCTCACCAT
CCAGGGGGAAAGCGACTTCTCCCTCTTCCTGCACCCCGACGGGCCACCCAAACCCCAGCA
GCTTCCTGAAAGCCCCAGCCGAGCACCACCCCTCGAGGACACAGAGGAAGAGAGAGGAGT
GACCATGGATCCACCAGTGAGTGAGGAGCGATCGGTGCAGCAGAGCCACCCCACTACCAC
CACCTCACCCCCCCGGCCTTACCCAGAGCTCATCTCTCGCCCCTCCCCACCTACCTTCCA
CCGGTTCCTGCCAGACTTGCCCCCATCCCGAAGTGCAGTGGAGATCGCCCCCACTCAGGT
CACAGAGACCGATGAGTGCCGATTGAACCAGAATATCTGTGGCCATGGACAGTGTGTGCC
TGGCCCCTCGGATTACTCCTGCCACTGCAACGCTGGCTACCGGTCACACCCGCAGCACCG
CTACTGTGTTGATGTGAACGAGTGCGAGGCAGAGCCCTGCGGCCCCGGGAAAGGCATCTG
TATGAACACTGGTGGCTCCTACAATTGTCACTGCAACCGAGGCTACCGCCTCCACGTGGG
TGCAGGGGGCCGCTCGTGCGTGGACCTGAACGAGTGCACCAAGCCTCACCTGTGTGGGGA
CGGTGGCTTCTGCATCAACTTCCCTGGTCACTACAAATGCAACTGCTATCCTGGCTACCG
GCTCAAGGCCTCCCGACCGCCCATTTGCGAAGACATCGACGAGTGTCGCGACCCTAGCAC
CTGCCCTGATGGCAAATGTGAAAACAAACCTGGCAGCTTCAAGTGCATCGCCTGCCAGCC
TGGCTACCGTAGCCAGGGGGGCGGGGCCTGTCGTGATGTCAACGAATGCTCCGAGGGTAC
CCCCTGCTCTCCTGGATGGTGTGAGAACCTTCCGGGTTCTTACCGTTGCACGTGTGCCCA
GGGATACGAACCCGCACAGGACGGCCTCAGTTGCATAGACGTGGATGAGTGTGAGGCTGG
GAAAGTGTGCCAAGATGGCATCTGCACGAACACACCAGGCTCTTTCCAGTGTCAGTGCCT
CTCCGGCTATCATCTGTCAAGGGATCGGAGCCGCTGTGAGGACATTGATGAATGTGACTT
CCCTGCGGCCTGCATCGGGGGTGACTGCATCAATACCAATGGTTCCTACAGATGTCTCTG
TCCCCAGGGTCATCGGTTGGTGGGCGGTAGGAAGTGCCAAGATATAGATGAGTGCAGCCA
GGACCCAGGCCTGTGCCTGCCCCACGGGGCCTGCGAGAACCTCCAGGGCTCCTATGTCTG
TGTCTGTGATGAGGGTTTCACACTCACCCAGGACCAGCATGGGTGTGAGGAGGTGGAGCA
GCCCCACCACAAGAAGGAGTGCTACCTTAACTTCGATGACACAGTGTTCTGTGACAGCGT
ATTGGCTACCAATGTCACTCAGCAGGAATGCTGTTGCTCTCTGGGAGCTGGTTGGGGAGA
CCACTGCGAAATCTATCCCTGTCCAGTCTACAGCTCAGCCGAATTTCACAGCCTGTGTCC
TGATGGGAAAGGCTACACTCAGGACAACAACATTGTGAACTATGGCATTCCTGCCCACCG
TGACATCGACGAATGCATATTGTTTGGGGCAGAGATCTGCAAGGAGGGCAAGTGTGTGAA
CACGCAGCCCGGCTACGAGTGCTACTGCAAGCAGGGCTTCTACTACGATGGCAACCTGCT
GGAGTGCGTGGACGTGGATGAGTGCTTGGATGAGTCTAACTGCAGGAACGGAGTGTGTGA
GAACACACGTGGCGGCTACCGCTGTGCCTGCACGCCGCCGGCAGAGTACAGTCCCGCACA
GCGCCAGTGTCTGAGCCCGGAGGAGATGGAGCACGCCCCAGAGAGACGTGAAGTGTGCTG
GGGCCAGCGAGGAGAGGACGGCATGTGTATGGGGCCCCTGGCGGGACCTGCCCTCACTTT
TGATGACTGCTGCTGCCGCCAGGGCCGCGGCTGGGGTACCCAGTGCAGACCGTGCCCGCC
ACGTGGCACCGGGTCCCAGTGCCCGACTTCACAGAGTGAGAGCAATTCATTCTGGGACAC
AAGCCCCCTGCTACTGGGGAAGTCTCCGCGAGACGAAGACAGCTCAGAGGAGGATTCAGA
TGAGTGCCGTTGTGTGAGCGGACGCTGTGTGCCACGGCCAGGCGGGGCGGTATGCGAGTG
TCCTGGAGGCTTTCAGCTGGACGCCTCCCGTGCCCGCTGCGTGGACATTGATGAGTGCCG
AGAACTGAACCAGCGGGGACTACTGTGTAAGAGCGAGCGGTGCGTGAACACCAGTGGATC
CTTCCGCTGTGTCTGCAAAGCTGGCTTCACGCGCAGCCGCCCTCACGGGGCCTGCGTGCC
TCAGCGCCGCCGCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MPGPRGAAHGLAPAMRQAGALGLLALLLLALLGPGGGAEGGPAGERGTGGGGALARERFK VVFAPVICKRTCLKGQCRDSCQQGSNMTLIGENGHSTDTLTGSGFRVVVCPLPCMNGGQC SSRNQCLCPPDFTGRFCQVPAAGTGAGTGSSGPGLARTGAMSTGPLPPLAPEGESVASKH AIYAVQVIADPPGPGEGPPAQHAAFLVPLGPGQISAEVQAPPPVVNVRVHHPPEASVQVH RIEGPNAEGPASSQHLLPHPKPQHPRPPTQKPLGRCFQDTLPKQPCGSNPLPGLTKQEDC CGSIGTAWGQSKCHKCPQLQYTGVQKPGPVRGEVGADCPQGYKRLNSTHCQDINECAMPG MCRHGDCLNNPGSYRCVCPPGHSLGPSRTQCIADKPEEKSLCFRLVSTEHQCQHPLTTRL TRQLCCCSVGKAWGARCQRCPADGTAAFKEICPAGKGYHILTSHQTLTIQGESDFSLFLH PDGPPKPQQLPESPSRAPPLEDTEEERGVTMDPPVSEERSVQQSHPTTTTSPPRPYPELI SRPSPPTFHRFLPDLPPSRSAVEIAPTQVTETDECRLNQNICGHGQCVPGPSDYSCHCNA GYRSHPQHRYCVDVNECEAEPCGPGKGICMNTGGSYNCHCNRGYRLHVGAGGRSCVDLNE CTKPHLCGDGGFCINFPGHYKCNCYPGYRLKASRPPICEDIDECRDPSTCPDGKCENKPG SFKCIACQPGYRSQGGGACRDVNECSEGTPCSPGWCENLPGSYRCTCAQGYEPAQDGLSC IDVDECEAGKVCQDGICTNTPGSFQCQCLSGYHLSRDRSRCEDIDECDFPAACIGGDCIN TNGSYRCLCPQGHRLVGGRKCQDIDECSQDPGLCLPHGACENLQGSYVCVCDEGFTLTQD QHGCEEVEQPHHKKECYLNFDDTVFCDSVLATNVTQQECCCSLGAGWGDHCEIYPCPVYS SAEFHSLCPDGKGYTQDNNIVNYGIPAHRDIDECILFGAEICKEGKCVNTQPGYECYCKQ GFYYDGNLLECVDVDECLDESNCRNGVCENTRGGYRCACTPPAEYSPAQRQCLSPEEMEH APERREVCWGQRGEDGMCMGPLAGPALTFDDCCCRQGRGWGTQCRPCPPRGTGSQCPTSQ SESNSFWDTSPLLLGKSPRDEDSSEEDSDECRCVSGRCVPRPGGAVCECPGGFQLDASRA RCVDIDECRELNQRGLLCKSERCVNTSGSFRCVCKAGFTRSRPHGACVPQRRR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4751
Start on reference sequence
990
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
