Hcn4 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Hcn4
Gene Name:  hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Original Clone ID:
FLH481119.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00294864
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3606nts
Open reading frame: 35 to 3640

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACAAGCTGCCGCCGTCCATGCG
CAAGCGGCTCTACAGCCTTCCGCAGCAGGTGGGGGCCAAGGCGTGGATCATGGACGAGGA
AGAGGATGGTGAGGAAGAAGGGGCCGGGGGCCGCCAGGACCCCAGCCGAAGGAGCATCCG
GCTGCGGCCGCTGCCCTCGCCCTCTCCCTCGGTGGCTGCGGGCTGCTCGGAGTCCCGGGG
TGCGGCCCTCGGGGCGACAGAGAGCGAGGGGCCGGGCCGCAGCGCCGGCAAGTCCAGCAC
CAACGGTGACTGCAGGCGCTTCCGCGGGAGTCTGGCCTCGCTGGGCAGCCGGGGCGGCGG
CAGTGGTGGAGCAGGGGGCGGCAGCAGTCTCGGGCACCTGCATGACTCCGCGGAGGAACG
GCGGCTCATCGCCGCTGAGGGCGATGCGTCCCCCGGCGAGGACAGGACGCCCCCGGGCCT
GGCGACCGAACCCGAGCGCCCGGCCACCGCGGCACAACCCGCAGCCTCGCCGCCGCCCCA
GCAGCCGCCGCAGCCGGCCTCTGCCTCCTGCGAGCAGCCCTCGGCGGACACCGCTATCAA
AGTGGAGGGAGGCGCGGCCGCCAGCGACCAGATCCTCCCCGAGGCCGAGGTGCGCCTGGG
CCAGAGCGGCTTCATGCAGCGCCAGTTCGGTGCCATGCTGCAACCTGGGGTCAACAAATT
CTCCCTAAGGATGTTCGGCAGCCAGAAAGCGGTGGAGCGCGAGCAGGAGAGGGTTAAGTC
AGCAGGGTTTTGGATTATCCACCCCTACAGTGACTTCAGATTTTACTGGGACCTGACGAT
GCTGTTGCTGATGGTGGGGAATCTGATCATCATACCCGTGGGCATCACCTTCTTCAAGGA
TGAGAACACCACACCCTGGATCGTCTTCAATGTGGTGTCAGACACATTCTTCCTCATTGA
CTTGGTCCTCAACTTCCGCACGGGGATCGTGGTGGAGGACAACACAGAAATCATCCTTGA
CCCGCAGAGGATCAAGATGAAGTACCTGAAAAGCTGGTTTGTGGTAGATTTCATCTCCTC
CATCCCTGTCGACTACATCTTCCTTATAGTGGAGACTCGCATTGACTCGGAGGTCTACAA
AACCGCTAGGGCTCTGCGCATTGTCCGTTTCACTAAGATCCTCAGCCTCCTGCGCCTCTT
GAGGCTTTCCCGCCTCATTCGATACATTCATCAGTGGGAAGAGATCTTCCACATGACCTA
TGACCTGGCCAGCGCCGTGGTACGCATCGTGAACCTCATTGGCATGATGCTTCTGCTGTG
TCACTGGGATGGCTGCCTGCAGTTCCTAGTGCCCATGCTGCAGGACTTCCCCCATGACTG
CTGGGTGTCCATCAATGGCATGGTGAATAACTCCTGGGGGAAGCAGTATTCCTACGCCCT
CTTCAAGGCCATGAGCCACATGCTGTGCATTGGGTATGGACGGCAGGCACCCGTAGGCAT
GTCTGACGTCTGGCTCACCATGCTCAGCATGATCGTGGGGGCCACCTGCTATGCCATGTT
CATCGGCCACGCCACTGCCCTCATCCAGTCGCTAGACTCCTCCCGGCGCCAGTACCAGGA
GAAGTATAAACAGGTGGAGCAGTACATGTCCTTCCACAAGCTCCCGCCTGACACCCGACA
GCGCATCCATGACTACTATGAACACCGCTACCAAGGCAAGATGTTTGATGAGGAAAGCAT
CCTGGGTGAGCTGAGTGAGCCACTTCGAGAGGAGATCATCAACTTTAACTGCCGAAAGCT
GGTGGCATCCATGCCACTGTTTGCCAACGCAGATCCCAACTTTGTGACATCCATGCTGAC
CAAGTTGCGTTTCGAGGTCTTCCAGCCTGGGGATTACATCATCCGCGAAGGCACCATCGG
CAAGAAGATGTACTTTATCCAGCACGGCGTGGTCAGCGTGCTCACTAAGGGCAACAAAGA
GACCAAGCTGGCTGATGGCTCCTATTTTGGAGAGATCTGCTTGCTGACCCGGGGTCGGCG
CACAGCCAGCGTCAGAGCGGATACTTATTGCCGCCTCTACTCACTGAGCGTGGACAACTT
CAATGAGGTGCTGGAGGAGTATCCCATGATGCGGAGGGCCTTCGAGACGGTTGCGCTGGA
CCGCCTGGACCGCATAGGCAAGAAGAACTCCATCCTCCTCCACAAGGTGCAGCACGACCT
CAACTCAGGCGTCTTCAACTACCAAGAGAACGAGATCATCCAGCAGATCGTGCGGCATGA
CCGTGAGATGGCCCACTGTGCTCACCGCGTCCAGGCTGCCGCCTCAGCCACCCCAACCCC
CACGCCTGTTATATGGACCCCGCTGATCCAGGCGCCACTGCAGGCTGCTGCTGCTACTAC
TTCGGTGGCCATAGCCCTCACACACCACCCCCGCCTGCCCGCCGCCATCTTCCGGCCCCC
TCCCGGACCTGGGCTGGGCAACCTTGGGGCTGGACAGACACCGAGGCACCCAAGGAGGCT
GCAGTCCTTGATCCCTTCAGCTCTGGGCTCTGCTTCACCCGCCAGCAGCCCCTCACAGGT
GGACACACCGTCTTCATCCTCCTTCCACATCCAACAGCTGGCTGGATTCTCTGCACCTCC
TGGATTGAGCCCTCTCCTGCCCTCCTCTAGCTCTTCCCCACCTCCAGGAGCCTGCGGTTC
CCCACCAGCCCCCACACCCTCCACCTCCACTGCCGCCGCCGCCTCCACCACTGGGTTCGG
CCACTTTCACAAGGCGCTGGGTGGCTCCCTGTCATCCTCTGACTCCCCGCTGCTCACCCC
ACTGCAACCAGGCGCTCGCTCTCCACAGGCTGCCCAGCCACCACCCCCACTGCCTGGGGC
CCGAGGAGGTCTGGGACTCCTGGAGCACTTCTTGCCGCCCCCACCCTCCTCCAGGTCACC
ATCATCCAGCCCTGGGCAGCTGGGCCAGCCTCCTGGAGAGTTGTCCCTAGGTCTGGCAGC
TGGTCCATCAAGTACACCAGAGACACCCCCACGGCCTGAGCGACCATCCTTCATGGCAGG
GGCCTCTGGAGGGGCTTCTCCTGTAGCCTTTACCCCCCGAGGAGGCCTCAGTCCTCCGGG
CCACAGCCCGGGGCCCCCAAGAACTTTCCCGAGTGCCCCACCCCGGGCCTCTGGCTCCCA
TGGTTCCCTGCTCCTGCCACCTGCATCCAGCCCTCCACCTCCCCAGGTCCCACAGCGCAG
GGGCACACCACCCCTCACCCCTGGCCGCCTCACACAGGACCTGAAGCTCATCTCAGCCTC
TCAGCCAGCCCTCCCCCAGGATGGGGCACAGACTCTCCGCAGGGCCTCGCCTCACTCCTC
AGGGGAGTCGGTGGCTGCCTTCTCACTCTACCCCAGAGCTGGGGGTGGCAGTGGGAGTAG
TGGGGGCCTTGGGCCTCCTGGAAGGCCATATGGTGCCATCCCAGGCCAACATGTCACTTT
GCCTCGGAAGACATCCTCAGGTTCTTTGCCACCCCCACTTTCTTTGTTTGGGGCAAGAGC
CGCCTCTTCTGGAGGGCCCCCTCTGACTACTGCTGCACCCCAGAGGGAACCTGGCGCTAG
GTCTGAGCCAGTACGCTCCAAACTGCCGTCTAATTTATAGGGCCTCATGGGCCCAGCTTT
CTTGTAC

Protein sequence predicted based on nucleotide sequence

MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDGEEEGAGGRQDPSRRSIRLRPLPSPSPSV
AAGCSESRGAALGATESEGPGRSAGKSSTNGDCRRFRGSLASLGSRGGGSGGAGGGSSLG
HLHDSAEERRLIAAEGDASPGEDRTPPGLATEPERPATAAQPAASPPPQQPPQPASASCE
QPSADTAIKVEGGAAASDQILPEAEVRLGQSGFMQRQFGAMLQPGVNKFSLRMFGSQKAV
EREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNV
VSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFVVDFISSIPVDYIFLIVE
TRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVN
LIGMMLLLCHWDGCLQFLVPMLQDFPHDCWVSINGMVNNSWGKQYSYALFKAMSHMLCIG
YGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSF
HKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANAD
PNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGE
ICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSI
LLHKVQHDLNSGVFNYQENEIIQQIVRHDREMAHCAHRVQAAASATPTPTPVIWTPLIQA
PLQAAAATTSVAIALTHHPRLPAAIFRPPPGPGLGNLGAGQTPRHPRRLQSLIPSALGSA
SPASSPSQVDTPSSSSFHIQQLAGFSAPPGLSPLLPSSSSSPPPGACGSPPAPTPSTSTA
AAASTTGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQAAQPPPPLPGARGGLGLLEHFL
PPPPSSRSPSSSPGQLGQPPGELSLGLAAGPSSTPETPPRPERPSFMAGASGGASPVAFT
PRGGLSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLT
QDLKLISASQPALPQDGAQTLRRASPHSSGESVAAFSLYPRAGGGSGSSGGLGPPGRPYG
AIPGQHVTLPRKTSSGSLPPPLSLFGARAASSGGPPLTTAAPQREPGARSEPVRSKLPSN
L*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 3606
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable