Gene:
Gene Symbol:
Dock9
Gene Name:
dedicator of cytokinesis 9
Original Clone ID:
FLH481121.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00294866
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 6342nts
Open reading frame: 35 to 6376
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCAGGCTGAGAAGTACAGGACGAG
CGGTAGAAGTGACAAGAAGGAGTTGGTCATCGAGTCCCCCCTGCAGTACAAGGATGCAGC
TCAGGCAGACGTGGAGGCAGAGAGCTCCACATTGGCAAAGCCAAAGCTGATCGAGCCACT
GGACTACGAAAATGTCATCGTGCAGAAGAAGACGCAGATCCTAAACGACTGCCTGCGGGA
GATGCTCCTCTTCCCTTATGATGACTTCCAGACGGCCATCCTGAGGCGGCAGGGGCGGTA
CTTACGCTCCACGGTCCCTGCAAATGCAGAGGAGGAAGCACAGAGCCTGTTTGTCACTGA
GTGCATCAAAACCTACAACTCTGACTGGCATCTTGTGACCTATAAATATGAAGATTACTC
AGGAGAGTTCCGACAGCTTCCAAACAAAGTGCCCAAGCTGGATAAACTTCCGGTCCACGT
CTATGAAGTCGATGAGGAGGCCGACAAAGATGAGGATGCTGCTTCCCTTGGGTCTCAGAA
GGGCGGGATCACCAAGCATGGCTGGCTGTACAAAGGCAACATGAACAGCGCCATCAGCGT
GACGATGAGGTCATTCAAGAGGCGGTTTTTCCACCTGATTCAACTTGGCGATGGATCCTA
TAATCTAAACTTTTATAAAGATGAGAAGATCTCCAAGGAACCGAAAGGGTCCATATTCCT
GGATTCCTGCATGGGTGTGATCCAGAACAACAGAGTCAGGCGCTTCGCTTTTGAGCTCAA
GATGCAAGACAAAAGCAGTTACCTTCTGGCGGCAGACAGCGAGGCAGAGATGGAGGAGTG
GGTCACTGTTCTCAACAAGATCCTCCAGCTCAACTTTGAAGCCGCAATGCAAGAAAAGCG
GAACGGGGATCCTCATGAAGATGACGAGCAGAGCAAACTGGAAGGTTCTGGTTCCGGTTT
GGACAGCTACCTGCCTGAACTTGCCAAGAGCACCAGAGAAGCAGAGATCAAATTGAAAAG
CGAGAGCAGAGTGAAGCTTTTTTACTTGGACCCAGATACCCAGAAACTTGACTTCTCATC
CGCTGAACCAGAAGTGAAGCCCTTTGAAGAAAAGTTTGGGAAAAGGATTCTCGTCAAGTG
CAACGATTTGTCCTTTAACCTGCAGTGCTGTGTCGCAGAGAATGAGGAAGGACCCACGAC
AAATGTCGAGCCTTTCTTTGTCACCCTGTCCCTGTTCGACATAAAATACAACCGAAAGAT
TTCTGCTGACTTCCACGTGGACCTGAACCACTTCTCAGTGCGGCAGATGCTGACCCCCAC
GTCCCCAGCTCTGATGAATGGTGGCCAGAGCCCACCTGCCTTCCAGGATGCCCTTCATAC
AGCCATGCAGTATCCGAAGCAGGGAATATTTTCAGTCACGTGTCCTCACCCAGACATATT
TCTTGTGGCCAGAATTGAGAAGGTCCTCCAAGGAAGCATCACGCACTGTGCTGAACCGTA
TATGAGAAGCTCAGACTCTTCTAAGGTTGCCCAGAAGGTGCTGAAGAATGCCAAGCAGGC
ATGCCAAAGACTAGGACAGTACAGAATGCCATTCGCCTGGGCAGCAAGGACGTTGTTTAA
AGACACCTCTGGAAACCTGGATAAAAACGCCAGATTTTCTGCCATCTACCGGCAAGACAG
CAATAAGCTTTCAAATGATGACATGCTCAAGCTGCTCGCAGACTTCCGGAAACCTGAGAA
GATGGCCAAACTCCCAGTGATTTTAGGCAATCTAGACATTACAATTGACAGTGTTTCCTG
TGACTTCCCTAATTATCTAAATTCATCATACATTCCCATGAGGCAATTTGAAACCTGCAG
TAAATCTCCAATCACTTTTGAAGTAGAGGAGTTTGTGCCCTGCATACCCAAGCACACCCA
GCCTTATACAGTCTACAGCAATCACCTTTATGTTTACCCAAAATACTTGAAATATGACAG
CCAGAAGTCGTTTGCCAAGGCCAGAAACATTGCTATCTGCATTGAGTTCAAGGATTCTGA
TGAAGAAGACTCTCAACCCTTGAAGTGCATTTACGGCAGACCTGGTGGCCCGGTGTTCAC
GAGAAGTGCCCTTGCCGCGGTCCTACACCATCAGCAAAACCCAGAATTCTACGATGAGAT
CAAGATAGAGCTGCCCGCCCAGCTGCATGAGAGGCACCATTTACTGTTCACCTTCTTCCA
CGTCAGCTGCGATAACTCCACCAAAGGAAGCACGAAGAAGAAGGACGCTGTGGAAACGCA
GGTTGGCTTTTCCTGGCTGCCTCTCCTGAAAGATGGAAGGGTGTTGACGAGTGAGCAGCA
CATCCCCGTCTCGGCTAACCTGCCATCTGGCTACCTCGGCTACCAGGAGCTCGGCATGGG
CAGGCATTATGGTCCAGAGGTTAAGTGGGTGGAAGGAGGCAAGCCACTGTTGAAGATCTC
CACTCATCTGGTTTCCACAGTGTACACTCAGGATCAACATTTACATAATTTTTTCCAATA
CTGTCAGAAAACGGAATCTGGAGCCCAAGCCTCAGGGAGTGAACTAGTAAAATACCTTAA
GAGTCTGCATGCGATGGAAGGCCATGTGATGATCGCCTTCTTGCCGACCATTTTAAATCA
GCTATTCAGAGTCCTCACAAGAGCCACCCAGGAGGAGGTTGCTGTGAACGTGACACGGGT
CATTATTCATGTGGTTGCCCAGTGCCATGAGGAAGGATTGGAGAGCCACTTGAGGTCATA
TGTTAAGTTTGCCTATAAGGCTGAGCCGTACGTTGCATCTGAGTATAAGACAGTGCACGA
GGAACTGACGAAATCCATGACCACCATTCTCAAGCCTTCTGCCGATTTCCTTACCAGCAA
CAAACTTCTGAAGTACTCTTGGTTTTTCTTTGATGTGCTGATAAAGTCCATGGCTCAGCA
TTTGATTGAAAATAACAAAGTCAAGTTACTGCGGAACCAGAGATTTCCGGCCTCCTACCA
TCACGCTGTGGAAACCGTGGTGAACATGCTGATGCCACATATCACCCAGAAATTTCGAGA
TAACCCAGAAGCATCTAAAAATGCCAATCACAGCCTCGCTGTGTTCATCAAGAGATGCTT
CACCTTCATGGACAGAGGCTTCGTCTTCAAGCAGATCAACAACTACATCAGTTGCTTTGC
TCCCGGGGACCCCAAGACTCTCTTTGAGTACAAGTTTGAGTTTCTCCGCGTGGTGTGCAA
CCATGAACACTATATTCCTTTGAATTTGCCGATGCCATTTGGAAAAGGAAGAATTCAGAG
ATACCAAGATCTCCAGCTTGACTACTCCTTAACAGACGAGTTCTGCAGAAACCACTTCTT
GGTGGGACTGTTGCTAAGGGAGGTGGGCACTGCGCTCCAGGAGTTCCGGGAGGTCCGAGT
CATCGCCATCAGCATGCTCAAGAACCTGCTGATAAAACATTCTTTTGATGACAGATACAA
TTCGAGGAGTCACCAGGCAAGGATAGCCACTCTCTACCTGCCTCTGTTTGGTCTGCTTAT
TGAAAATGTCCAGCGGATCAACGTGAGGGATGTGTCACCCTTTCCTGTGAACCCGGGCAG
TATCGTGAAGGACGAAGCCCTGGCTGTGCCTGCTGGGAATCCACTCATGACTCCGCAGAA
GGGAAACACACTTGACCACAGCCTGCACAAAGACCTCTTGGGCGCCATCTCTGGCATTGC
TTCTCCGTATACAGCCTCAACCCCCAACATCAACAGCGTGAGAAATGCCGACTCAAGAGG
CTCTCTCATTAGCACGGACTCAGGGAACAGCCTTCCAGACAGGAACCCCGAGAAGAGCAA
CTCTCTGGATAAGCAGCAGAGTGGCATGCTGGGAAATTCCGTGGTTCGATGCGACAAACT
GGACCAGTCTGAGATCAAGAGCCTGCTGATGTGTTTCCTCTACGTGCTGAAAAGCATGTC
TGACGATGCCCTGTTTACATATTGGAACAAAGCTTCAACTGCTGAATTGATGGATTTCTT
TACAATATCTGAGGTCTGCCTGCACCAGTTCCAGTACATGGGGAAGCGATACATAGCCAG
GAACCAGGAGGGGTTGGGACCCATAGGTCATGATCGAAAGTCTCAGACATTGCCTGTTTC
CCGTAACAGAACAGGAATGATGCATGCCAGATTGCAGCAGCTGGGCAGCCTGGATAACTC
TGTCACTTTTAACCACAGCTACGGCCACTCAGAGGCAGATGTCGTTCACCAGTCGCTTCT
CGAAGCCAACATCGCTACTGAGGTCTGCCTCACAGCGCTGGACACCCTCTCTCTCTTCAC
ACTGGCTTTTAAGAACCAGCTCCTAGCTGATCATGGGCATAATCCCCTCATGAAGAAAGT
TTTTGACGTCTACCTGTGTTTCCTTCAAAAACACCAGTCAGAAATGGCTTTAAAAAACGT
CTTTACTGCCTTAAGGTCTCTAATTTATAAGTTCCCCTCGGCGTTCTACGAGGGGCGGGC
GGACATGTGTGCCTCCCTGTGCTATGAGGTCCTCAAGTGCTGCAACTCCAAGCTCAGTTC
CATCCGGACGGAGGCCTCCCAGCTGCTCTACTTCCTGATGAGGAACAACTTCGACTACAC
AGGAAAGAAGTCTTTTGTTCGGACGCACTTACAGGTCATCATCTCTGTCAGCCAACTGAT
TGCAGATGTGGTTGGCATTGGAGGAACCAGATTCCAGCAGTCCTTGTCTATCATCAACAA
CTGTGCCAACAGCGACCGGATCATCAAGCACACCAGCTTTTCCTCTGATGTGAAAGATTT
GACTAAGAGGATCCGCACAGTCCTGATGGCCACAGCCCAGATGAAGGAGCACGAGAACGA
CCCGGAGATGCTGGTGGACCTCCAGTACAGCCTGGCTAAGTCCTACGCCAGCACCCCTGA
GCTCAGGAAGACGTGGCTAGACAGTATGGCGAGGATTCACGTTAAAAATGGGGACCTCTC
AGAGGCGGCAATGTGCTATGTCCACGTGACAGCCTTGGTGGCAGAATATCTCACACGGAA
AGAAGCTGACCTAGCACTCCAGCGGGAACCACCTGCCTTCCCCTACAGCCATAGCACCTG
CCAGAGGAAGAGCTGGGGAGGCATGTTCAGACAGGGGTGCACAGCCTTCAGGGTTATCAC
ACCAAACATCGATGAAGAGGCTTCCATGATGGAAGACGTCGGCATGCAGGACGTCCATTT
CAACGAGGATGTGCTGATGGAGCTGCTGGAGCAGTGTGCGGATGGACTTTGGAAGGCGGA
GCGCTACGAGCTGATCGCTGACATCTATAAGCTCATCATCCCCATCTACGAAAAGCGGAG
GGATTTCGAGAGACTAGCCCATCTGTATGACACGCTGCACCGCGCATACAGCAAAGTGAC
AGAGGTCATGCACTCGGGCCGCAGGCTCCTGGGGACCTACTTCCGGGTGGCCTTCTTTGG
ACAGGGGTTCTTCGAAGACGAAGATGGGAAGGAATACATCTACAAAGAGCCCAAGCTCAC
GCCTCTGTCAGAGATTTCTCAGAGACTCCTTAAACTTTACTCGGATAAATTTGGTTCCGA
AAATGTCAAAATGATACAGGATTCTGGCAAGGTCAACCCGAAGGATCTGGACTCCAAGTT
TGCCTACATCCAGGTGACCCACGTGACCCCATTCTTTGACGAAAAGGAGTTACAAGAGAG
GAGAACAGAGTTTGAACGATGTCACAACATCCGGCGCTTCATGTTTGAGATGCCCTTCAC
CCAGACCGGGAAGAGGCAGGGTGGCGTGGAGGAGCAGTGTAAGCGGCGGACCATCCTGAC
AGCAATACACTGCTTCCCCTACGTGAAGAAGCGGATCCCTGTCATGTACCAGCACCACAC
TGACCTGAACCCCATTGAGGTGGCCATCGATGAAATGAGCAAGAAGGTGGCCGAGCTCCG
CCAGCTCTGCTCGTCGGCTGAAGTGGATATGATCAAACTGCAGCTCAAACTGCAGGGCAG
TGTGAGCGTCCAGGTCAATGCTGGTCCGCTAGCATATGCCCGAGCCTTCCTCGATGACAC
CAACACAAAAAGATACCCTGACAATAAGGTGAAACTGCTGAAGGAAGTTTTCAGGCAATT
TGTGGAAGCTTGTGGTCAAGCCTTGGCAGTGAACGAGCGTCTCATTAAAGAAGACCAACT
GGAGTACCAGGAAGAGATGAAGGCCAACTACAGGGAGATGGCCAAGGAGCTCTCCGACAT
CATGCGTGAGCAGATTTGCCCCCTGGAGGAGAAGACAAGCGTGCTACCAAATTCCCTGCA
CATCTTCAACGCCATCAGCGGGACACCAACAAGCACAGTGGTTCAAGGGTTGACCAGCTC
GTCCTCAGTTGTGTAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MQAEKYRTSGRSDKKELVIESPLQYKDAAQADVEAESSTLAKPKLIEPLDYENVIVQKKT QILNDCLREMLLFPYDDFQTAILRRQGRYLRSTVPANAEEEAQSLFVTECIKTYNSDWHL VTYKYEDYSGEFRQLPNKVPKLDKLPVHVYEVDEEADKDEDAASLGSQKGGITKHGWLYK GNMNSAISVTMRSFKRRFFHLIQLGDGSYNLNFYKDEKISKEPKGSIFLDSCMGVIQNNR VRRFAFELKMQDKSSYLLAADSEAEMEEWVTVLNKILQLNFEAAMQEKRNGDPHEDDEQS KLEGSGSGLDSYLPELAKSTREAEIKLKSESRVKLFYLDPDTQKLDFSSAEPEVKPFEEK FGKRILVKCNDLSFNLQCCVAENEEGPTTNVEPFFVTLSLFDIKYNRKISADFHVDLNHF SVRQMLTPTSPALMNGGQSPPAFQDALHTAMQYPKQGIFSVTCPHPDIFLVARIEKVLQG SITHCAEPYMRSSDSSKVAQKVLKNAKQACQRLGQYRMPFAWAARTLFKDTSGNLDKNAR FSAIYRQDSNKLSNDDMLKLLADFRKPEKMAKLPVILGNLDITIDSVSCDFPNYLNSSYI PMRQFETCSKSPITFEVEEFVPCIPKHTQPYTVYSNHLYVYPKYLKYDSQKSFAKARNIA ICIEFKDSDEEDSQPLKCIYGRPGGPVFTRSALAAVLHHQQNPEFYDEIKIELPAQLHER HHLLFTFFHVSCDNSTKGSTKKKDAVETQVGFSWLPLLKDGRVLTSEQHIPVSANLPSGY LGYQELGMGRHYGPEVKWVEGGKPLLKISTHLVSTVYTQDQHLHNFFQYCQKTESGAQAS GSELVKYLKSLHAMEGHVMIAFLPTILNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEE GLESHLRSYVKFAYKAEPYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFD VLIKSMAQHLIENNKVKLLRNQRFPASYHHAVETVVNMLMPHITQKFRDNPEASKNANHS LAVFIKRCFTFMDRGFVFKQINNYISCFAPGDPKTLFEYKFEFLRVVCNHEHYIPLNLPM PFGKGRIQRYQDLQLDYSLTDEFCRNHFLVGLLLREVGTALQEFREVRVIAISMLKNLLI KHSFDDRYNSRSHQARIATLYLPLFGLLIENVQRINVRDVSPFPVNPGSIVKDEALAVPA GNPLMTPQKGNTLDHSLHKDLLGAISGIASPYTASTPNINSVRNADSRGSLISTDSGNSL PDRNPEKSNSLDKQQSGMLGNSVVRCDKLDQSEIKSLLMCFLYVLKSMSDDALFTYWNKA STAELMDFFTISEVCLHQFQYMGKRYIARNQEGLGPIGHDRKSQTLPVSRNRTGMMHARL QQLGSLDNSVTFNHSYGHSEADVVHQSLLEANIATEVCLTALDTLSLFTLAFKNQLLADH GHNPLMKKVFDVYLCFLQKHQSEMALKNVFTALRSLIYKFPSAFYEGRADMCASLCYEVL KCCNSKLSSIRTEASQLLYFLMRNNFDYTGKKSFVRTHLQVIISVSQLIADVVGIGGTRF QQSLSIINNCANSDRIIKHTSFSSDVKDLTKRIRTVLMATAQMKEHENDPEMLVDLQYSL AKSYASTPELRKTWLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRKEADLALQREPP AFPYSHSTCQRKSWGGMFRQGCTAFRVITPNIDEEASMMEDVGMQDVHFNEDVLMELLEQ CADGLWKAERYELIADIYKLIIPIYEKRRDFERLAHLYDTLHRAYSKVTEVMHSGRRLLG TYFRVAFFGQGFFEDEDGKEYIYKEPKLTPLSEISQRLLKLYSDKFGSENVKMIQDSGKV NPKDLDSKFAYIQVTHVTPFFDEKELQERRTEFERCHNIRRFMFEMPFTQTGKRQGGVEE QCKRRTILTAIHCFPYVKKRIPVMYQHHTDLNPIEVAIDEMSKKVAELRQLCSSAEVDMI KLQLKLQGSVSVQVNAGPLAYARAFLDDTNTKRYPDNKVKLLKEVFRQFVEACGQALAVN ERLIKEDQLEYQEEMKANYREMAKELSDIMREQICPLEEKTSVLPNSLHIFNAISGTPTS TVVQGLTSSSSVV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
105
End on reference sequence
6446
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
