DNASU Plasmid | ALX4 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: ALX4

Gene Name: ALX homeobox 4

Original Clone ID:

FLH481142.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00294887

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1233nts

Open reading frame: 1 to 1233

Reference Sequence Alignment

ATGAATGCTGAGACTTGCGTCTCTTACTGCGAGTCGCCGGCCGCTGCCATGGACGCCTAC
TACAGCCCGGTGTCGCAGAGTCGGGAGGGCTCGTCGCCTTTTAGGGCATTTCCCGGAGGC
GACAAGTTCGGCACAACTTTCCTGTCGGCCGCCGCCAAAGCACAGGGATTCGGGGACGCC
AAGAGCCGGGCCCGTTACGGCGCTGGGCAGCAGGACCTGGCGACACCCCTGGAGAGTGGA
GCTGGGGCGCGGGGCTCCTTTAACAAGTTCCAGCCCCAGCCGTCGACCCCGCAGCCCCAG
CCGCCGCCGCAGCCGCAGCCGCAGCAGCAGCAGCCGCAGCCCCAGCCGCCCGCGCAACCG
CATCTTTACTTGCAGCGAGGCGCCTGCAAGACGCCCCCGGACGGCAGCCTCAAACTCCAG
GAAGGCAGCAGCGGCCACAGCGCGGCCTTGCAGGTTCCCTGCTACGCTAAAGAGAGCTCC
CTGGGTGAGCCAGAGTTACCCCCTGACTCTGACACTGTGGGGATGGACAGCAGCTACCTG
AGTGTCAAGGAGGCTGGGGTGAAGGGGCCCCAGGACCGGGCCAGCTCAGACCTCCCCAGC
CCATTGGAGAAGGCCGACTCAGAGAGCAACAAGGGCAAGAAGCGGCGGAACCGGACCACC
TTCACCAGCTACCAGCTGGAGGAGCTGGAGAAGGTCTTCCAGAAGACCCACTACCCAGAC
GTGTATGCGCGGGAACAGCTGGCCATGAGGACAGACCTCACTGAGGCCCGCGTGCAGGTC
TGGTTCCAGAACCGAAGGGCCAAGTGGAGGAAGCGGGAGCGTTTTGGGCAGATGCAGCAG
GTTCGAACCCACTTCTCCACTGCATATGAGCTGCCCCTCCTCACCCGAGCTGAGAACTAC
GCCCAGATTCAGAACCCGTCCTGGCTCGGCAACAACGGGGCTGCCTCACCAGTGCCAGCC
TGCGTGGTCCCCTGCGACCCGGTGCCTGCCTGCATGTCCCCTCATGCCCACCCCCCTGGC
TCTGGGGCCAGCAGCGTCACCGACTTCCTGAGTGTGTCTGGGGCTGGCAGTCACGTGGGC
CAGACGCACATGGGCAGCCTGTTTGGAGCAGCCAGCCTCAGCCCAGGCCTCAATGGCTAC
GAGCTCAACGGCGAGCCGGACCGCAAGACCTCGAGCATCGCGGCCCTCCGCATGAAGGCC
AAGGAGCACAGTGCGGCCATTTCCTGGGCCACA

Protein sequence predicted based on nucleotide sequence

MNAETCVSYCESPAAAMDAYYSPVSQSREGSSPFRAFPGGDKFGTTFLSAAAKAQGFGDA
KSRARYGAGQQDLATPLESGAGARGSFNKFQPQPSTPQPQPPPQPQPQQQQPQPQPPAQP
HLYLQRGACKTPPDGSLKLQEGSSGHSAALQVPCYAKESSLGEPELPPDSDTVGMDSSYL
SVKEAGVKGPQDRASSDLPSPLEKADSESNKGKKRRNRTTFTSYQLEELEKVFQKTHYPD
VYAREQLAMRTDLTEARVQVWFQNRRAKWRKRERFGQMQQVRTHFSTAYELPLLTRAENY
AQIQNPSWLGNNGAASPVPACVVPCDPVPACMSPHAHPPGSGASSVTDFLSVSGAGSHVG
QTHMGSLFGAASLSPGLNGYELNGEPDRKTSSIAALRMKAKEHSAAISWAT

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 105

End on reference sequence 1340

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

ALX4

Symbol Nomenclature :

ALX4

SYNONYM :

CRS5; FND2

Designation :

aristaless-like homeobox 4|homeobox protein aristaless-like 4|homeodomain transcription factor ALX4

Full Nomenclature :

ALX homeobox 4

GENEID :

60529

GI :

187252472

GenBank Accession :

BC166622

HGNC :

450

MIM :

605420

Vega :

OTTHUMG00000166557

Target GenBank:

NM_021926.2

Reference Sequence Alignment

  

HsCD00294887      GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAATGCTGAGACTTGCGTCTCTTA
NM_021926.3       ----------------------------------ATGAATGCTGAGACTTGCGTCTCTTA
                                                    **************************

HsCD00294887      CTGCGAGTCGCCGGCCGCTGCCATGGACGCCTACTACAGCCCGGTGTCGCAGAGTCGGGA
NM_021926.3       CTGCGAGTCGCCGGCCGCTGCCATGGACGCCTACTACAGCCCGGTGTCGCAGAGTCGGGA
                  ************************************************************

HsCD00294887      GGGCTCGTCGCCTTTTAGGGCATTTCCCGGAGGCGACAAGTTCGGCACAACTTTCCTGTC
NM_021926.3       GGGCTCGTCGCCTTTTAGGGCATTTCCCGGAGGCGACAAGTTCGGCACAACTTTCCTGTC
                  ************************************************************

HsCD00294887      GGCCGCCGCCAAAGCACAGGGATTCGGGGACGCCAAGAGCCGGGCCCGTTACGGCGCTGG
NM_021926.3       GGCCGCCGCCAAAGCACAGGGATTCGGGGACGCCAAGAGCCGGGCCCGTTACGGCGCTGG
                  ************************************************************

HsCD00294887      GCAGCAGGACCTGGCGACACCCCTGGAGAGTGGAGCTGGGGCGCGGGGCTCCTTTAACAA
NM_021926.3       GCAGCAGGACCTGGCGACACCCCTGGAGAGTGGAGCTGGGGCGCGGGGCTCCTTTAACAA
                  ************************************************************

HsCD00294887      GTTCCAGCCCCAGCCGTCGACCCCGCAGCCCCAGCCGCCGCCGCAGCCGCAGCCGCAGCA
NM_021926.3       GTTCCAGCCCCAGCCGTCGACCCCGCAGCCCCAGCCGCCGCCGCAGCCGCAGCCGCAGCA
                  ************************************************************

HsCD00294887      GCAGCAGCCGCAGCCCCAGCCGCCCGCGCAACCGCATCTTTACTTGCAGCGAGGCGCCTG
NM_021926.3       GCAGCAGCCGCAGCCCCAGCCGCCCGCGCAACCGCATCTTTACTTGCAGCGAGGCGCCTG
                  ************************************************************

HsCD00294887      CAAGACGCCCCCGGACGGCAGCCTCAAACTCCAGGAAGGCAGCAGCGGCCACAGCGCGGC
NM_021926.3       CAAGACGCCCCCGGACGGCAGCCTCAAACTCCAGGAAGGCAGCAGCGGCCACAGCGCGGC
                  ************************************************************

HsCD00294887      CTTGCAGGTTCCCTGCTACGCTAAAGAGAGCTCCCTGGGTGAGCCAGAGTTACCCCCTGA
NM_021926.3       CTTGCAGGTTCCCTGCTACGCTAAAGAGAGCTCCCTGGGTGAGCCAGAGTTACCCCCTGA
                  ************************************************************

HsCD00294887      CTCTGACACTGTGGGGATGGACAGCAGCTACCTGAGTGTCAAGGAGGCTGGGGTGAAGGG
NM_021926.3       CTCTGACACTGTGGGGATGGACAGCAGCTACCTGAGTGTCAAGGAGGCTGGGGTGAAGGG
                  ************************************************************

HsCD00294887      GCCCCAGGACCGGGCCAGCTCAGACCTCCCCAGCCCATTGGAGAAGGCCGACTCAGAGAG
NM_021926.3       GCCCCAGGACCGGGCCAGCTCAGACCTCCCCAGCCCATTGGAGAAGGCCGACTCAGAGAG
                  ************************************************************

HsCD00294887      CAACAAGGGCAAGAAGCGGCGGAACCGGACCACCTTCACCAGCTACCAGCTGGAGGAGCT
NM_021926.3       CAACAAGGGCAAGAAGCGGCGGAACCGGACCACCTTCACCAGCTACCAGCTGGAGGAGCT
                  ************************************************************

HsCD00294887      GGAGAAGGTCTTCCAGAAGACCCACTACCCAGACGTGTATGCGCGGGAACAGCTGGCCAT
NM_021926.3       GGAGAAGGTCTTCCAGAAGACCCACTACCCAGACGTGTATGCGCGGGAACAGCTGGCCAT
                  ************************************************************

HsCD00294887      GAGGACAGACCTCACTGAGGCCCGCGTGCAGGTCTGGTTCCAGAACCGAAGGGCCAAGTG
NM_021926.3       GAGGACAGACCTCACTGAGGCCCGCGTGCAGGTCTGGTTCCAGAACCGAAGGGCCAAGTG
                  ************************************************************

HsCD00294887      GAGGAAGCGGGAGCGTTTTGGGCAGATGCAGCAGGTTCGAACCCACTTCTCCACTGCATA
NM_021926.3       GAGGAAGCGGGAGCGTTTTGGGCAGATGCAGCAGGTTCGAACCCACTTCTCCACTGCATA
                  ************************************************************

HsCD00294887      TGAGCTGCCCCTCCTCACCCGAGCTGAGAACTACGCCCAGATTCAGAACCCGTCCTGGCT
NM_021926.3       TGAGCTGCCCCTCCTCACCCGAGCTGAGAACTACGCCCAGATTCAGAACCCGTCCTGGCT
                  ************************************************************

HsCD00294887      CGGCAACAACGGGGCTGCCTCACCAGTGCCAGCCTGCGTGGTCCCCTGCGACCCGGTGCC
NM_021926.3       CGGCAACAACGGGGCTGCCTCACCAGTGCCAGCCTGCGTGGTCCCCTGCGACCCGGTGCC
                  ************************************************************

HsCD00294887      TGCCTGCATGTCCCCTCATGCCCACCCCCCTGGCTCTGGGGCCAGCAGCGTCACCGACTT
NM_021926.3       TGCCTGCATGTCCCCTCATGCCCACCCCCCTGGCTCTGGGGCCAGCAGCGTCACCGACTT
                  ************************************************************

HsCD00294887      CCTGAGTGTGTCTGGGGCTGGCAGTCACGTGGGCCAGACGCACATGGGCAGCCTGTTTGG
NM_021926.3       CCTGAGTGTGTCTGGGGCTGGCAGTCACGTGGGCCAGACGCACATGGGCAGCCTGTTTGG
                  ************************************************************

HsCD00294887      AGCAGCCAGCCTCAGCCCAGGCCTCAATGGCTACGAGCTCAACGGCGAGCCGGACCGCAA
NM_021926.3       AGCAGCCAGCCTCAGCCCAGGCCTCAATGGCTACGAGCTCAACGGCGAGCCGGACCGCAA
                  ************************************************************

HsCD00294887      GACCTCGAGCATCGCGGCCCTCCGCATGAAGGCCAAGGAGCACAGTGCGGCCATTTCCTG
NM_021926.3       GACCTCGAGCATCGCGGCCCTCCGCATGAAGGCCAAGGAGCACAGTGCGGCCATTTCCTG
                  ************************************************************

HsCD00294887      GGCCACATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
NM_021926.3       GGCCACATGA---------------------------
                  ********..