DNASU Plasmid | GHRHR (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: GHRHR

Gene Name: growth hormone releasing hormone receptor

Original Clone ID:

FLH481156.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00294901

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1272nts

Open reading frame: 35 to 1306

Reference Sequence Alignment

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACCGCCGGATGTGGGGGGCCCA
CGTCTTCTGCGTGTTGAGCCCGTTACCGACCGTATTGGGCCACATGCACCCAGAATGTGA
CTTCATCACCCAGCTGAGAGAGGATGAGAGTGCCTGTCTACAAGCAGCAGAGGAGATGCC
CAACACCACCCTGGGCTGCCCTGCGACCTGGGATGGGCTGCTGTGCTGGCCAACGGCAGG
CTCTGGCGAGTGGGTCACCCTCCCCTGCCCGGATTTCTTCTCTCACTTCAGCTCAGAGTC
AGGGGCTGTGAAACGGGATTGTACTATCACTGGCTGGTCTGAGCCCTTTCCACCTTACCC
TGTGGCCTGCCCTGTGCCTCTGGAGCTGCTGGCTGAGGAGGAATCTTACTTCTCCACAGT
GAAGATTATCTACACCGTGGGCCATAGCATCTCTATTGTAGCCCTCTTCGTGGCCATCAC
CATCCTGGTTGCTCTCAGGAGGCTCCACTGCCCCCGGAACTACGTCCACACCCAGCTGTT
CACCACTTTTATCCTCAAGGCGGGAGCTGTGTTCCTGAAGGATGCTGCCCTTTTCCACAG
CGACGACACTGACCACTGCAGCTTCTCCACTGTTCTATGCAAGGTCTCTGTGGCCGCCTC
CCATTTCGCCACCATGACCAACTTCAGCTGGCTGTTGGCAGAAGCCGTCTACCTGAACTG
CCTCCTGGCCTCCACCTCCCCCAGCTCAAGGAGAGCCTTCTGGTGGCTGGTTCTCGCTGG
CTGGGGGCTGCCCGTGCTCTTCACTGGCACGTGGGTGAGCTGCAAACTGGCCTTCGAGGA
CATCGCGTGCTGGGACCTGGACGACACCTCCCCCTACTGGTGGATCATCAAAGGGCCCAT
TGTCCTCTCGGTCGGGGTGAACTTTGGGCTTTTTCTCAATATTATCCGCATCCTGGTGAG
GAAACTGGAGCCAGCTCAGGGCAGCCTCCATACCCAGTCTCAGTATTGGCGTCTCTCCAA
GTCGACACTTTTCCTGATCCCACTCTTTGGAATTCACTACATCATCTTCAACTTCCTGCC
AGACAATGCTGGCCTGGGCATCCGCCTCCCCCTGGAGCTGGGACTGGGTTCCTTCCAGGG
CTTCATTGTTGCCATCCTCTACTGCTTCCTCAACCAAGAGGTGAGGACTGAGATCTCACG
GAAGTGGCATGGCCATGACCCTGAGCTTCTGCCAGCCTGGAGGACCCGTGCTAAGTGGAC
CACGCCTTCCCGCTCGGCGGCAAAGGTGCTGACATCTATGTGCTAGGGCCTCATGGGCCC
AGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MDRRMWGAHVFCVLSPLPTVLGHMHPECDFITQLREDESACLQAAEEMPNTTLGCPATWD
GLLCWPTAGSGEWVTLPCPDFFSHFSSESGAVKRDCTITGWSEPFPPYPVACPVPLELLA
EEESYFSTVKIIYTVGHSISIVALFVAITILVALRRLHCPRNYVHTQLFTTFILKAGAVF
LKDAALFHSDDTDHCSFSTVLCKVSVAASHFATMTNFSWLLAEAVYLNCLLASTSPSSRR
AFWWLVLAGWGLPVLFTGTWVSCKLAFEDIACWDLDDTSPYWWIIKGPIVLSVGVNFGLF
LNIIRILVRKLEPAQGSLHTQSQYWRLSKSTLFLIPLFGIHYIIFNFLPDNAGLGIRLPL
ELGLGSFQGFIVAILYCFLNQEVRTEISRKWHGHDPELLPAWRTRAKWTTPSRSAAKVLT
SMC*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1320

Start on reference sequence 49

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

GHRHR

Symbol Nomenclature :

GHRHR

SYNONYM :

GHRFR; GRFR; IGHD1B

Designation :

GHRH receptor|GRF receptor|growth hormone-releasing hormone receptor

Full Nomenclature :

growth hormone releasing hormone receptor

GENEID :

2692

GI :

187252484

GenBank Accession :

BC166636

HGNC :

4266

MIM :

139191

Vega :

OTTHUMG00000152801

Target GenBank:

NM_000823.2

Reference Sequence Alignment

  

HsCD00294901      GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGACCGCCGGATGTGGGGGGCCCA
NM_000823.3       ----------------------------------ATGGACCGCCGGATGTGGGGGGCCCA
                                                    **************************

HsCD00294901      CGTCTTCTGCGTGTTGAGCCCGTTACCGACCGTATTGGGCCACATGCACCCAGAATGTGA
NM_000823.3       CGTCTTCTGCGTGTTGAGCCCGTTACCGACCGTATTGGGCCACATGCACCCAGAATGTGA
                  ************************************************************

HsCD00294901      CTTCATCACCCAGCTGAGAGAGGATGAGAGTGCCTGTCTACAAGCAGCAGAGGAGATGCC
NM_000823.3       CTTCATCACCCAGCTGAGAGAGGATGAGAGTGCCTGTCTACAAGCAGCAGAGGAGATGCC
                  ************************************************************

HsCD00294901      CAACACCACCCTGGGCTGCCCTGCGACCTGGGATGGGCTGCTGTGCTGGCCAACGGCAGG
NM_000823.3       CAACACCACCCTGGGCTGCCCTGCGACCTGGGATGGGCTGCTGTGCTGGCCAACGGCAGG
                  ************************************************************

HsCD00294901      CTCTGGCGAGTGGGTCACCCTCCCCTGCCCGGATTTCTTCTCTCACTTCAGCTCAGAGTC
NM_000823.3       CTCTGGCGAGTGGGTCACCCTCCCCTGCCCGGATTTCTTCTCTCACTTCAGCTCAGAGTC
                  ************************************************************

HsCD00294901      AGGGGCTGTGAAACGGGATTGTACTATCACTGGCTGGTCTGAGCCCTTTCCACCTTACCC
NM_000823.3       AGGGGCTGTGAAACGGGATTGTACTATCACTGGCTGGTCTGAGCCCTTTCCACCTTACCC
                  ************************************************************

HsCD00294901      TGTGGCCTGCCCTGTGCCTCTGGAGCTGCTGGCTGAGGAGGAATCTTACTTCTCCACAGT
NM_000823.3       TGTGGCCTGCCCTGTGCCTCTGGAGCTGCTGGCTGAGGAGGAATCTTACTTCTCCACAGT
                  ************************************************************

HsCD00294901      GAAGATTATCTACACCGTGGGCCATAGCATCTCTATTGTAGCCCTCTTCGTGGCCATCAC
NM_000823.3       GAAGATTATCTACACCGTGGGCCATAGCATCTCTATTGTAGCCCTCTTCGTGGCCATCAC
                  ************************************************************

HsCD00294901      CATCCTGGTTGCTCTCAGGAGGCTCCACTGCCCCCGGAACTACGTCCACACCCAGCTGTT
NM_000823.3       CATCCTGGTTGCTCTCAGGAGGCTCCACTGCCCCCGGAACTACGTCCACACCCAGCTGTT
                  ************************************************************

HsCD00294901      CACCACTTTTATCCTCAAGGCGGGAGCTGTGTTCCTGAAGGATGCTGCCCTTTTCCACAG
NM_000823.3       CACCACTTTTATCCTCAAGGCGGGAGCTGTGTTCCTGAAGGATGCTGCCCTTTTCCACAG
                  ************************************************************

HsCD00294901      CGACGACACTGACCACTGCAGCTTCTCCACTGTTCTATGCAAGGTCTCTGTGGCCGCCTC
NM_000823.3       CGACGACACTGACCACTGCAGCTTCTCCACTGTTCTATGCAAGGTCTCTGTGGCCGCCTC
                  ************************************************************

HsCD00294901      CCATTTCGCCACCATGACCAACTTCAGCTGGCTGTTGGCAGAAGCCGTCTACCTGAACTG
NM_000823.3       CCATTTCGCCACCATGACCAACTTCAGCTGGCTGTTGGCAGAAGCCGTCTACCTGAACTG
                  ************************************************************

HsCD00294901      CCTCCTGGCCTCCACCTCCCCCAGCTCAAGGAGAGCCTTCTGGTGGCTGGTTCTCGCTGG
NM_000823.3       CCTCCTGGCCTCCACCTCCCCCAGCTCAAGGAGAGCCTTCTGGTGGCTGGTTCTCGCTGG
                  ************************************************************

HsCD00294901      CTGGGGGCTGCCCGTGCTCTTCACTGGCACGTGGGTGAGCTGCAAACTGGCCTTCGAGGA
NM_000823.3       CTGGGGGCTGCCCGTGCTCTTCACTGGCACGTGGGTGAGCTGCAAACTGGCCTTCGAGGA
                  ************************************************************

HsCD00294901      CATCGCGTGCTGGGACCTGGACGACACCTCCCCCTACTGGTGGATCATCAAAGGGCCCAT
NM_000823.3       CATCGCGTGCTGGGACCTGGACGACACCTCCCCCTACTGGTGGATCATCAAAGGGCCCAT
                  ************************************************************

HsCD00294901      TGTCCTCTCGGTCGGGGTGAACTTTGGGCTTTTTCTCAATATTATCCGCATCCTGGTGAG
NM_000823.3       TGTCCTCTCGGTCGGGGTGAACTTTGGGCTTTTTCTCAATATTATCCGCATCCTGGTGAG
                  ************************************************************

HsCD00294901      GAAACTGGAGCCAGCTCAGGGCAGCCTCCATACCCAGTCTCAGTATTGGCGTCTCTCCAA
NM_000823.3       GAAACTGGAGCCAGCTCAGGGCAGCCTCCATACCCAGTCTCAGTATTGGCGTCTCTCCAA
                  ************************************************************

HsCD00294901      GTCGACACTTTTCCTGATCCCACTCTTTGGAATTCACTACATCATCTTCAACTTCCTGCC
NM_000823.3       GTCGACACTTTTCCTGATCCCACTCTTTGGAATTCACTACATCATCTTCAACTTCCTGCC
                  ************************************************************

HsCD00294901      AGACAATGCTGGCCTGGGCATCCGCCTCCCCCTGGAGCTGGGACTGGGTTCCTTCCAGGG
NM_000823.3       AGACAATGCTGGCCTGGGCATCCGCCTCCCCCTGGAGCTGGGACTGGGTTCCTTCCAGGG
                  ************************************************************

HsCD00294901      CTTCATTGTTGCCATCCTCTACTGCTTCCTCAACCAAGAGGTGAGGACTGAGATCTCACG
NM_000823.3       CTTCATTGTTGCCATCCTCTACTGCTTCCTCAACCAAGAGGTGAGGACTGAGATCTCACG
                  ************************************************************

HsCD00294901      GAAGTGGCATGGCCATGACCCTGAGCTTCTGCCAGCCTGGAGGACCCGTGCTAAGTGGAC
NM_000823.3       GAAGTGGCATGGCCATGACCCTGAGCTTCTGCCAGCCTGGAGGACCCGTGCTAAGTGGAC
                  ************************************************************

HsCD00294901      CACGCCTTCCCGCTCGGCGGCAAAGGTGCTGACATCTATGTGCTAGGGCCTCATGGGCCC
NM_000823.3       CACGCCTTCCCGCTCGGCGGCAAAGGTGCTGACATCTATGTGCTAG--------------
                  **********************************************              

HsCD00294901      AGCTTTCTTGTAC
NM_000823.3       -------------

Pathways

Reactome :

Class B/2 (Secretin family receptors)

Reactome :

G alpha (s) signalling events

Reactome :

Glucagon-type ligand receptors

Reactome :

GPCR downstream signaling

Reactome :

GPCR ligand binding

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

WikiPathway :

GPCRs, Class B Secretin-like

WikiPathway :

GPCRs, Other

Protein

SMART domain :

HormR : Domain present in hormone receptors

UniProt :

Q02643

HPRD :

00748

Clone

Cloning Information :

481156

HIP Master Clone ID :

398884

IMAGE ID :

100066362

Original Clone ID :

FLH481156.01X

Organism

Species Specific ID:

2692

Genome

Chromosome :

Map Location :

7p14

Ensembl :

ENSG00000106128

Reagents

Labome :

GHRHR-antibody