DNASU Plasmid | PIK3R6 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: PIK3R6

Gene Name: phosphoinositide-3-kinase, regulatory subunit 6

Original Clone ID:

FLH481165.01X

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pENTR223.1

Format:

FUSION

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

HsCD00294910

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2262nts

Open reading frame: 1 to 2262

ATGGAGAGCTCAGATGTGGAGCTGGACCTCCAGAGGAGCGTGCAGGCTGTGCTCCGGGAG
CTCAGCACCCAGGCCCCTGCCCTGCAGAGCAACCAAGGCATGTGGAGGTGGTCCCTGCAC
AAGAAGGTCGAGCGAGATCCCGGTAAGAGCCCAGTGCTGGTCCGCATTCTTCTCAGAGAA
CTGGAGAAGGCGGAAAGCCAGGACCTCCGGCATGTCATCATTCCCTTGCTGCACACTGTA
ATGTACGTGCTCACCAAGGCCACAGGAATCACAGAAGAGCTCTACCAGAGAATCTATGCC
TTTTGCACAAGGTTACTGACCCTGCCCACCCCCTACTGCACAGTCGCCTTGGACTGCGCG
ATAAGGCTGAAAACGGAGATGGCTGTCCCAGGGACACTGTACCAAAGGATGGTCATTGCC
GAACAGAACTTGACGAATGAGCTGTATCCCTACCAGGAGAGAGTGTTCCTCTTCGTGGAT
CCTGAGCTGGTGTCTGCGTCTGTGTGCAGTGCTCTGCTACTGGAGATCGAGGCGGCCCAG
GCGCAGCAGACACCAGAGACCTGCATGCGCCACGTGGTCTCCCACGCCCTGCAGGCGGCT
CTGGGGGAGGCCTGTCACGCAGGCGCTCTGCACAGGAAGCTGCAGGCCAGCCCTCGCCGC
ACCCTGGAGCACTATTTCCACGCCGTGGTGGCCGCCTTGGAGCAGATGGCCAGCGAGGCC
AGCCCGAGCCGGGAGGGACACGTAGAGAGGCTGGAGGAGATTTACTGCTCGCTGCTGGGT
CCCGCGGCGGGGCGCTGCGGTGGTGACCTTGTCCAAGAGCGGCCACCAAGCATTCCCCTG
CCCAGCCCCTACATCACCTTCCACTTGTGGACCGGTGAGGAGCAGCTCTGGAAGGAACTG
GTGCTCTTCCTCCGCCCAAGATCCCAGCTGCGCCTCAGTGCTGACTTGGAGGTCTTGGAT
CTGCAGGGCCTCCGGCCGGACCGGGAGTTGGCCCGGGTTTCTGTGCTGTCCACTGACAGC
GGCATTGAGCGGGACCTTCCCACGGGGGCTGATGAGCTGCCTGCACCCGGCAGCCCTGAG
ATGGAGCGAGCCGGGCTGCAGCGCAAAGGGGGCATCAAGAAGCGTGCATGGCCCCTGGAC
TTCTTGATGCCTGGCAGCTGGGACGGGCCCCCAGGGCTGCACCGGAGGACAGGCCGGCCC
AGTGGGGATGGGGAAATGCTGCCCGGCGTGTCCCGGCTGCACACAGCCCGGGTACTTGTG
CTCGGAGATGACAGGATGCTGGGGCGCCTGGCCCAGGCCTACCACAGACTCAGGAAACGG
GAGACCCAGAAGTTCTGCCTCACTCCCAGACTCAGCCTGCAGCTCTACTACATCCCCGTG
CTGGCGCCTGAGAAGCCTGCAGCATCCAGGCAGCCGGAGCTGGGAGAGCTGGCTACGTTC
CTGGGCCGCGTAGACCCGTGGTACCAGAGCAACGTCAACACGCTGTGCCCCGCCATCCAC
AAGCTGGCTGAGATGCCTCCTTCCCTGGACACATCCCGGACTGTGGACCCCTTCATCCTA
GACGTCATCACCTACTACATCCGCATGGGCACCCAACCCATCTATTTCCAGATCTACACA
GTCAAGATCTTTTTCAGTGACCTGAGCCAAGACCCTACTGAGGACATTTTCCTCATTGAA
CTGAAGGTGAAGATCCAAGATTCTAAATTCCCCAAAGATGGCTTTTCACCCAGGAGGAGA
GGCGTGGCTGAGGGCCCAGGGGCAGAGCTCTCCCTATGCTACCAGAAGGCCTTGCTTAGC
CACCGGCCCCGAGAGGTCACCGTTTCCCTGCGGGCCACTGGGCTGATCCTGAAGGCCATT
CCAGCCAGCGACACAGAAGTTTCAGGGTCTAGCCATTGCCCCCTGCCTGCTGCTCCTGTC
ACAGACCACACATGTCTGAATGTCAACGTGACAGAGGTTGTCAAGTCCTCCAACTTGGCG
GGAAAGTCCTTCTCTACAGTGACCAACACCTTCAGGACGAACAATATCCAGATCCAGAGC
CGGGACCAGAGGCTGCTGACACTGTCGCTGGACAAGGACGATCAACGCACTTTCAGGGAT
GTGGTCAGATTCGAGGTTGCTCCCTGCCCAGAACCATGTTCTGGGGCCCAGAAGTCCAAG
GCACCGTGGCTCAATTTGCATGGGCAACAGGAGGTGGAAGCAATCAAAGCCAAGCCCAAG
CCCCTTCTGATGCCCATCAACACATTCTCTGGTATTGTCCAG

Protein sequence predicted based on nucleotide sequence

MESSDVELDLQRSVQAVLRELSTQAPALQSNQGMWRWSLHKKVERDPGKSPVLVRILLRE
LEKAESQDLRHVIIPLLHTVMYVLTKATGITEELYQRIYAFCTRLLTLPTPYCTVALDCA
IRLKTEMAVPGTLYQRMVIAEQNLTNELYPYQERVFLFVDPELVSASVCSALLLEIEAAQ
AQQTPETCMRHVVSHALQAALGEACHAGALHRKLQASPRRTLEHYFHAVVAALEQMASEA
SPSREGHVERLEEIYCSLLGPAAGRCGGDLVQERPPSIPLPSPYITFHLWTGEEQLWKEL
VLFLRPRSQLRLSADLEVLDLQGLRPDRELARVSVLSTDSGIERDLPTGADELPAPGSPE
MERAGLQRKGGIKKRAWPLDFLMPGSWDGPPGLHRRTGRPSGDGEMLPGVSRLHTARVLV
LGDDRMLGRLAQAYHRLRKRETQKFCLTPRLSLQLYYIPVLAPEKPAASRQPELGELATF
LGRVDPWYQSNVNTLCPAIHKLAEMPPSLDTSRTVDPFILDVITYYIRMGTQPIYFQIYT
VKIFFSDLSQDPTEDIFLIELKVKIQDSKFPKDGFSPRRRGVAEGPGAELSLCYQKALLS
HRPREVTVSLRATGLILKAIPASDTEVSGSSHCPLPAAPVTDHTCLNVNVTEVVKSSNLA
GKSFSTVTNTFRTNNIQIQSRDQRLLTLSLDKDDQRTFRDVVRFEVAPCPEPCSGAQKSK
APWLNLHGQQEVEAIKAKPKPLLMPINTFSGIVQ

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

Start on reference sequence 241

End on reference sequence 2505

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

PIK3R6

Symbol Nomenclature :

PIK3R6

SYNONYM :

C17orf38; HsT41028; p84; p84 PIKAP; p87(PIKAP); p87PIKAP

Designation :

PI3Kgamma adapter protein of 87 kDa|p84 PI3K adapter protein|p87 phosphoinositide 3-kinase gamma (PI3Kg) adapter protein|phosphoinositide 3-kinase regulatory subunit 6

Full Nomenclature :

phosphoinositide-3-kinase, regulatory subunit 6

GENEID :

146850

GI :

187252554

GenBank Accession :

BC166645

HGNC :

27101

MIM :

611462

Target GenBank:

NM_001010855.2

Pathways

NCI :

Class I PI3K signaling events

NCI :

Class IB PI3K non-lipid kinase events

NCI :

CXCR4-mediated signaling events

NCI :

EPHA forward signaling

NCI :

IL8- and CXCR1-mediated signaling events

NCI :

IL8- and CXCR2-mediated signaling events

NCI :

PDGFR-beta signaling pathway

NCI :

Thromboxane A2 receptor signaling

Reactome :

G beta:gamma signalling through PI3Kgamma

Reactome :

G-protein beta:gamma signalling

Reactome :

GPCR downstream signaling

Reactome :

GPVI-mediated activation cascade

Reactome :

Hemostasis

Reactome :

Metabolism

Reactome :

Metabolism of lipids and lipoproteins

Reactome :

Phospholipid metabolism

Reactome :

PI Metabolism

Reactome :

Platelet activation, signaling and aggregation

Reactome :

Signal Transduction

Reactome :

Signaling by GPCR

Reactome :

Synthesis of PIPs at the plasma membrane

Protein

UniProt :

Q5UE93

HPRD :

12682

Clone

Cloning Information :

481165

HIP Master Clone ID :

398893

IMAGE ID :

100066371

Original Clone ID :

FLH481165.01X

Organism

Species Specific ID:

146850

Genome

Chromosome :

Map Location :

17p13.1

Ensembl :

ENSG00000174083

Reagents

Labome :

PIK3R6-antibody